LOCUS       sequence-246473-        6911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SomArchon under CaMKII promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Piatkevich KD, Bensussen S, Tseng HA, Shroff SN, Lopez-Huerta VG, 
            Park D, Jung EE, Shemesh OA, Straub C, Gritton HJ, Romano MF, Costa 
            E, Sabatini BL, Fu Z, Boyden ES, Han X
  TITLE     Population imaging of neural activity in awake behaving mice.
  JOURNAL   Nature. 2019 Oct;574(7778):413-417. doi: 10.1038/s41586-019-1641-1. 
            Epub 2019 Oct 9.
  PUBMED    31597963
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1641-1"; journalName: "Nature"; date: "2019-10";
            volume: "574"; issue: "7778"; pages: "413-417"
COMMENT     Sequence Label: pAAV-CaMKII-SomArchon
FEATURES             Location/Qualifiers
     source          1..6911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   35..164
                     /label=AAV2 ITR
     promoter        237..1525
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      1535..1544
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2408..2410,2411..3121)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2408..2410,2411..3121)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2450..2471)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2711..2730)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3058..3079
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3389..3977
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3442..3462)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3860..3871)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4020..4141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4031..4050)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4085..4104
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4303..4432
                     /label=AAV2 ITR
     rep_origin      complement(4669..5257)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4749..4768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5428..6219,6220..6288))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6051..6070
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6289..6393)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6419..6874)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6556..6577)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6768..6787
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcgatcggtg cgggcctctt cgctattacg ccagctgcgc gctcgctcgc tcactgaggc
       61 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
      121 agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa
      181 cccgccatgc tacttatcta cgtagccatg ctctaggaag atcgtaggtt aattaacatt
      241 atggccttag gtcacttcat ctccatgggg ttcttcttct gattttctag aaaatgagat
      301 gggggtgcag agagcttcct cagtgacctg cccagggtca catcagaaat gtcagagcta
      361 gaacttgaac tcagattact aatcttaaat tccatgcctt gggggcatgc aagtacgata
      421 tacagaagga gtgaactcat tagggcagat gaccaatgag tttaggaaag aagagtccag
      481 ggcagggtac atctacacca cccgcccagc cctgggtgag tccagccacg ttcacctcat
      541 tatagttgcc tctctccagt cctaccttga cgggaagcac aagcagaaac tgggacagga
      601 gccccaggag accaaatctt catggtccct ctgggaggat gggtggggag agctgtggca
      661 gaggcctcag gaggggccct gctgctcagt ggtgacagat aggggtgaga aagcagacag
      721 agtcattccg tcagcattct gggtctgttt ggtacttctt ctcacgctaa ggtggcggtg
      781 tgatatgcac aatggctaaa aagcagggag agctggaaag aaacaaggac agagacagag
      841 gccaagtcaa ccagaccaat tcccagagga agcaaagaaa ccattacaga gactacaagg
      901 gggaagggaa ggagagatga attagcttcc cctgtaaacc ttagaaccca gctgttgcca
      961 gggcaacggg gcaatacctg tctcttcaga ggagatgaag ttgccagggt aactacatcc
     1021 tgtctttctc aaggaccatc ccagaatgtg gcacccacta gccgttacca tagcaactgc
     1081 ctctttgccc cacttaatcc catcccgtct gttaaaaggg ccctatagtt ggaggtgggg
     1141 gaggtaggaa gagcgatgat cacttgtgga ctaagtttgt tcgcatcccc ttctccaacc
     1201 ccctcagtac atcaccctgg gggaacaggg tccacttgct cctgggccca cacagtcctg
     1261 cagtattgtg tatataaggc cagggcaaag aggagcaggt tttaaagtga aaggcaggca
     1321 ggtgttgggg aggcagttac cggggcaacg ggaacagggc gtttcggagg tggttgccat
     1381 ggggacctgg atgctgacga aggctcgcga ggctgtgagc agccacagtg ccctgctcag
     1441 aagccccaag ctcgtcagtc aagccggttc tccgtttgca ctcaggagca cgggcaggcg
     1501 agtggcccct agttctgggg gcagcggggg atccgccacc atggtttcta tcgcactgca
     1561 ggctggttat gacctcctcg gagatggtag acctgaaagt ctgtggctcg gaattggtac
     1621 actgctgatg ctgatcggaa ccttctactt cctggttaga gcgtggggag agactgacaa
     1681 ggatgcacgg gagtactacg cagtgacaat cctggtcagc ggtattgcca gtgcagctta
     1741 tctgtcaatg ttctttggta ttggcctcac agaggtgcca gtaggtggag agatgctgaa
     1801 catctactac gcaagatacg cacagtggct gttcactaca ccactgttgc tgctgcacct
     1861 ggcactcctg gccaaagttg atagagtgac catcggcact ctcgttggcg tcgatgctct
     1921 gatgattgtc acaggcctga taggaacact gtcacatact gccattgcca gatactcttg
     1981 gtggctgttc tcaactatct gtatgatcgt cgttctgtat gtcctggcca cttctctgcg
     2041 gtcagccgct aaggagagag gcccagaggt tgcctcaacc ttcaacatcc tcaccgcact
     2101 ggttctggtc ctgtggactg cttaccctat catctggatt atcggcaccg agggtgccgg
     2161 agttgtgggc ctgggaatcg aaactttgct gttcatggtg ctggacgtca ccgccaaggt
     2221 gggcttcggt tttatcctgc tcaggtcaag agccattctg caagatacag aagcacctga
     2281 accttcagct ggagctgata ccggtgccgc cgaccgcccg gtagtagcag tgagcaaggc
     2341 ggccgccaag agcaggatca ccagcgaggg cgagtacatc cccctggacc agatcgacat
     2401 caacgtggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2461 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2521 ctacggcaag ctgaccctga agttcatttg caccaccggc aagctgcccg tgccctggcc
     2581 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2641 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2701 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2761 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2821 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     2881 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2941 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3001 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3061 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3121 gggtgggagc ggaggtaccc agagccagcc tatcctgaac accaaggaga tggcccctca
     3181 gagcaagcct cctgaggagc tggagatgag cagcatgcct agccctgtgg cccctctgcc
     3241 tgccaggacc gagggcgtga tcgacatgag gagcatgagc agcatcgaca gcttcatcag
     3301 ctgcgccacc gacttccctg aggccaccag gttctacaag ttctgctacg agaacgaggt
     3361 gtaagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     3421 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     3481 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     3541 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     3601 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     3661 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     3721 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3781 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     3841 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3901 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3961 ttgggccgcc tccccgcatc gataccgtcg acccgggcgg ccgcttcgag cagacatgat
     4021 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4081 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4141 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     4201 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatcttc ctagagcatg
     4261 gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg
     4321 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     4381 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcatta
     4441 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     4501 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4561 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4621 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4681 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4741 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4801 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4861 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4921 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4981 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5041 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5101 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5161 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5221 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5281 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5341 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5401 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5461 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5521 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     5581 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5641 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5701 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5761 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5821 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5881 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5941 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6001 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6061 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6121 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6181 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6241 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6301 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6361 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     6421 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     6481 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     6541 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     6601 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     6661 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     6721 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     6781 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     6841 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     6901 tgttgggaag g
//
