LOCUS       sequence-246481-       16421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16421)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX034
FEATURES             Location/Qualifiers
     source          1..16421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6567..6591)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6620..6709
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6743..6995
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7424..7676)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8144..9169)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8144..9169)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9179..9523)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9633..9650)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9633..9649)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9642..9664)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9789..9808)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9999..10020
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10267..10291
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11887..13035)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13334..14128)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14320..15087
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15780..16411
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgactaa
     4621 tatatggagc aatactacat ccgatgataa tatgatggaa gcttttttat cttctgatcc
     4681 gtcgtcgttt tgggctggaa ctactactac accaactcct cggagttcag tttctccggc
     4741 gccggcgccg gtgacgggga ttgccgtaga cccattaaca tctatgccat atttcaacca
     4801 agagtcactg caacagcgac ttcagacttt aatcgacggg gctcgcgaag cgtggacgta
     4861 tgccatattc tggcaatcgt ctgttgtgga tttcacgacc cactcggttt tggggtgggg
     4921 agatgggtat tataaaggtg aagaagataa aaataagcgc aaaacggcgt cgttttcgcc
     4981 tgattttatc acggagcaag cacaccggaa aaaggttctc cgggagctga attgtttaat
     5041 ttccggcaca caaactggtg gtgaaaatga tgctgtagat gaagaagtaa cggatactga
     5101 atggtttttt ctgatttcca tgactcaatc gttcgttaac ggaagcgggc ttccgggcct
     5161 ggcgatgtac agctcaagcc cgatttgggt tactggagca gagagattag ctgcttcgca
     5221 ctgtgaacgg gcccgacagg cccaaggttt cgggcttcag actattgttt gtattccttc
     5281 aggtaatggt gttgttgagc tcgggtcaac tgagttgata ttccagactg ctgatttaat
     5341 gaacaaggtt aaagttttgt ttaattttaa tattgatatg ggtgcgacta cgggctcagg
     5401 atcgggctca tgtgctattc aggccgagcc cgatacttca gccctttggc tgacggatcc
     5461 agcttcctca gctgtggaag tcaaggattc gtctaataca gttccttcaa gtaatagcag
     5521 taagcaactt gtgtttggaa atgagaattc tgaaaatggt aatcaaaatt ctcagcaaac
     5581 acaaggattt ttcaccaggg agttgaattt ttccgaatat ggatttgatg gaagtaatac
     5641 tcggaatggg aatgtgaatt cttcgcgttc ttgccagcct gagtctggtg aaatcttgaa
     5701 ttttggtgat agtactaaga gaagtgcttc aagtgcaaat gggagcttgt tttcgggcca
     5761 atcacagttt gggcccgggc ccgcggagga gaacaagaac aagaacaaga aaaggtcacc
     5821 tgcatcaaga ggaagcaacg atgaaggaat gctttcattt gtttcgggtg tgattttgcc
     5881 aagttcaaac acggggaagt ctggtggagg tggcgattcg gatcaatcag atctcgaggc
     5941 ttcggtggtg aaggaagcgg atagtagtag agttgtagac ccggagaaga agccgaggaa
     6001 acgagggagg aaaccggcta acgggagaga ggagccattg aatcatgtgg aggcagagag
     6061 gcaaaggagg gagaaattaa atcaaagatt ctatgcactt agagcagttg taccaaatgt
     6121 gtcaaaaatg gataaagcat cacttcttgg tgatgcaatt gcatttatca atgagttgaa
     6181 atcaaaggtt cagaattctg actcagataa agaggagttg aggaaccaaa ttgaatcttt
     6241 aaggaatgaa ttagccaaca agggatcaaa ctataccggt cctccaccgt taaatcaaga
     6301 actcaagatt gtagatatgg atatcgacgt taaggtgatc ggatgggatg ctatgattcg
     6361 tatacaatct aataaaaaaa accatccagc cgcgaagtta atggccgctc tcatggaatt
     6421 ggacttagat gtgcaccatg ctagtgtttc agtggtcaac gagttgatga tccaacaagc
     6481 aactgtgaaa atggggagtc ggctttacac gcaagaacaa cttcggatat cattgacatc
     6541 tagaattgct gaatcgcgac ccggggaccc agctttcttg tacaaagtgg ttgataacag
     6601 cgggttaatt aacatctttt acccatacga tgttcctgac tatgcgggct atccctatga
     6661 cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg ctcagtgcag
     6721 cgcttagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     6781 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     6841 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     6901 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     6961 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttagcttcat caacgcaaga
     7021 catgcgcacg accgtctgac aggagaggaa tttccgacga gcacagaaag gacttgctct
     7081 tggacgtagg cctatttctc aggcacatgt atcaagtgtt cggacgtggg ttttcgatgg
     7141 tgtatcagcc gccgccaact gggagatgag gaggctttct tggggggcag tcagcagttc
     7201 atttcacaag acagaggaac ttgtaaggag atgcactgat ttatcttggc gcaaaccagc
     7261 aggacgaatt agtgggaata gcccgcgaat atctaagtta tgcctgtcgg catgagcaga
     7321 aacttccaat tcgaaacagt ttggagaggt tgtttttggg catacctttt gttagtcagc
     7381 ctctcgattg ctcatcgtca ttacacagta ccgaagtttg atcgatctag taacatagat
     7441 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
     7501 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
     7561 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
     7621 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatctgct
     7681 tcgacgcact ccttctttac tccaccatct cgtccttatt gaaaacgtgg gtagcaccaa
     7741 aacgaatcaa gtcgctggaa ctgaagttac caatcacgct ggatgatttg ccagttggat
     7801 taatcttgcc tttccccgca tgaataatat tgatgaatgc atgcgtgagg ggtatttcga
     7861 ttttggcaat agctgcaatt gccgcgacat cctccaacga gcataattct tcagaaaaat
     7921 agcgatgttc catgttgtca gggcatgcat gatgcacgtt atgaggtgac ggtgctaggc
     7981 agtattccct caaagtttca tagtcagtat catattcatc attgcattcc tgcaagagag
     8041 aattgagacg caatccacac gctgcggcaa ccttccggcg ttcgtggtct atttgctctt
     8101 ggacgttgca aacgtaagtg ttggatcccg gtcggcatct actctattcc tttgccctcg
     8161 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     8221 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     8281 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     8341 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
     8401 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     8461 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     8521 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     8581 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     8641 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     8701 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     8761 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
     8821 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     8881 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca
     8941 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     9001 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     9061 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     9121 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tcggggtcgt
     9181 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     9241 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
     9301 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     9361 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
     9421 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
     9481 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
     9541 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttgac
     9601 ggatctctag gacgcgtcct agaagctaat tcactggccg tcgttttaca acgtcgtgac
     9661 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     9721 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     9781 ggcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     9841 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     9901 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     9961 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    10021 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
    10081 ggaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
    10141 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa
    10201 agaaaaacca ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
    10261 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
    10321 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
    10381 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc
    10441 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa
    10501 cgatgacaga gcgttgctgc ctgtgatcaa atatcatctc cctcgcagag atccgaatta
    10561 tcagccttct tattcatttc tcgcttaacc gtgacaggct gtcgatcttg agaactatgc
    10621 cgacataata ggaaatcgct ggataaagcc gctgaggaag ctgagtggcg ctatttcttt
    10681 agaagtgaac gttgacgata tcaactcccc tatccattgc tcaccgaatg gtacaggtcg
    10741 gggacccgaa gttccgactg tcggcctgat gcatccccgg ctgatcgacc ccagatctgg
    10801 ggctgagaaa gcccagtaag gaaacaactg taggttcgag tcgcgagatc ccccggaacc
    10861 aaaggaagta ggttaaaccc gctccgatca ggccgagcca cgccaggccg agaacattgg
    10921 ttcctgtagg catcgggatt ggcggatcaa acactaaagc tactggaacg agcagaagtc
    10981 ctccggccgc cagttgccag gcggtaaagg tgagcagagg cacgggaggt tgccacttgc
    11041 gggtcagcac ggttccgaac gccatggaaa ccgcccccgc caggcccgct gcgacgccga
    11101 caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc cacgcccgca gttccgcaaa
    11161 tagcccccag gaccgccatc aatcgtatcg ggctacctag cagagcggca gagatgaaca
    11221 cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac cccgcccggc aggcggtaga
    11281 ccgaaataaa caacaagctc cagaatagcg aaatattaag tgcgccgagg atgaagatgc
    11341 gcatccacca gattcccgtt ggaatctgtc ggacgatcat cacgagcaat aaacccgccg
    11401 gcaacgcccg cagcagcata ccggcgaccc ctcggcctcg ctgttcgggc tccacgaaaa
    11461 cgccggacag atgcgccttg tgagcgtcct tggggccgtc ctcctgtttg aagaccgaca
    11521 gcccaatgat ctcgccgtcg atgtaggcgc cgaatgccac ggcatctcgc aaccgttcag
    11581 cgaacgcctc catgggcttt ttctcctcgt gctcgtaaac ggacccgaac atctctggag
    11641 ctttcttcag ggccgacaat cggatctcgc ggaaatcctg cacgtcggcc gctccaagcc
    11701 gtcgaatctg agccttaatc acaattgtca attttaatcc tctgtttatc ggcagttcgt
    11761 agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag cagtgcccgc
    11821 ttgttcctga aatgccagta aagcgctggc tgctgaaccc ccagccggaa ctgaccccac
    11881 aaggccctag cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc
    11941 gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt
    12001 ggaatccgat ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg
    12061 cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca
    12121 tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac
    12181 ctggcaacgg gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac
    12241 cgattccagg tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga
    12301 caggcattcc tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca
    12361 aagctcgtag aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa
    12421 cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat
    12481 cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt
    12541 cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt
    12601 gtccggccac ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt
    12661 gtgtttcagc aacgcggcct gcttggcctc gctgacctgt tttgccaggt cctcgccggc
    12721 ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa
    12781 acctgccgcc tcctgttcga gacgacgcga acgctccacg gcggccgatg gcgcgggcag
    12841 ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat
    12901 cgagccgacg gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt
    12961 ttcggcatcc tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc
    13021 aaacgtccga ttcattcacc ctccttgcgg gattgccccg actcacgccg gggcaatgtg
    13081 cccttattcc tgatttgacc cgcctggtgc cttggtgtcc agataatcca ccttatcggc
    13141 aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg tacttggtat tccgaatctt
    13201 gccctgcacg aataccagcg accccttgcc caaatacttg ccgtgggcct cggcctgaga
    13261 gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccgg catcgttgcg
    13321 ccacatctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
    13381 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
    13441 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
    13501 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
    13561 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
    13621 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
    13681 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
    13741 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
    13801 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
    13861 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
    13921 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
    13981 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
    14041 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
    14101 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
    14161 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
    14221 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
    14281 catagtatcg acggagccga ttttgaaacc acaattatgg gtgatgctgc caacttactg
    14341 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
    14401 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg
    14461 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
    14521 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
    14581 agcccgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
    14641 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
    14701 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
    14761 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
    14821 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
    14881 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
    14941 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
    15001 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
    15061 cactatcaat aagttggagt cattacccaa ttatgataga atttacaagc tataaggtta
    15121 ttgtcctggg tttcaagcat tagtccatgc aagtttttat gctttgccca ttctatagat
    15181 atattgataa gcgcgctgcc tatgccttgc cccctgaaat ccttacatac ggcgatatct
    15241 tctatataaa agatatatta tcttatcagt attgtcaata tattcaaggc aatctgcctc
    15301 ctcatcctct tcatcctctt cgtcttggta gctttttaaa tatggcgctt catagagtaa
    15361 ttctgtaaag gtccaattct cgttttcata cctcggtata atcttaccta tcacctcaaa
    15421 tggttcgctg ggtttatcgc acccccgaac acgagcacgg cacccgcgac cactatgcca
    15481 agaatgccca aggtaaaaat tgccggcccc gccatgaagt ccgtgaatgc cccgacggcc
    15541 gaagtgaagg gcaggccgcc acccaggccg ccgccctcac tgcccggcac ctggtcgctg
    15601 aatgtcgatg ccagcacctg cggcacgtca atgcttccgg gcgtcgcgct cgggctgatc
    15661 gcccatcccg ttactgcccc gatcccggca atggcaagga ctgccagcgc tgccattttt
    15721 ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg gggatgggag gcccgcgtta
    15781 gcgggccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca
    15841 cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa
    15901 gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc
    15961 tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc
    16021 aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg
    16081 cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc
    16141 gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc
    16201 ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca
    16261 tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc cgcggtgtct
    16321 cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc
    16381 agcggcgagg gcaaccagcc cggtgagcgt cggaaaggcg c
//
