LOCUS       sequence-246516-       16462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16462)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX042
FEATURES             Location/Qualifiers
     source          1..16462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6576..6600)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6629..6718
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6752..7004
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7433..7685)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8153..9178)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8153..9178)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9188..9532)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9674..9691)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9674..9690)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9683..9705)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9830..9849)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10040..10061
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10308..10332
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11928..13076)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13375..14169)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14361..15128
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15821..16452
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg acggattgta gaagaccaac
     4621 gatgactaat atatggagca atactacatc cgatgataat atgatggaag cttttttatc
     4681 ttctgatccg tcgtcgtttt gggctggaac tactactaca ccaactcctc ggagttcagt
     4741 ttctccggcg ccggcgccgg tgacggggat tgccgtagac ccattaacat ctatgccata
     4801 tttcaaccaa gagtcactgc aacagcgact tcagacttta atcgacgggg ctcgcgaagc
     4861 gtggacgtat gccatattct ggcaatcgtc tgttgtggat ttcacgaccc actcggtttt
     4921 ggggtgggga gatgggtatt ataaaggtga agaagataaa aataagcgca aaacggcgtc
     4981 gttttcgcct gattttatca cggagcaagc acaccggaaa aaggttctcc gggagctgaa
     5041 ttgtttaatt tccggcacac aaactggtgg tgaaaatgat gctgtagatg aagaagtaac
     5101 ggatactgaa tggttttttc tgatttccat gactcaatcg ttcgttaacg gaagcgggct
     5161 tccgggcctg gcgatgtaca gctcaagccc gatttgggtt actggagcag agagattagc
     5221 tgcttcgcac tgtgaacggg cccgacaggc ccaaggtttc gggcttcaga ctattgtttg
     5281 tattccttca ggtaatggtg ttgttgagct cgggtcaact gagttgatat tccagactgc
     5341 tgatttaatg aacaaggtta aagttttgtt taattttaat attgatatgg gtgcgactac
     5401 gggctcagga tcgggctcat gtgctattca ggccgagccc gatacttcag ccctttggct
     5461 gacggatcca gcttcctcag ctgtggaagt caaggattcg tctaatacag ttccttcaag
     5521 taatagcagt aagcaacttg tgtttggaaa tgagaattct gaaaatggta atcaaaattc
     5581 tcagcaaaca caaggatttt tcaccaggga gttgaatttt tccgaatatg gatttgatgg
     5641 aagtaatact cggaatggga atgtgaattc ttcgcgttct tgccagcctg agtctggtga
     5701 aatcttgaat tttggtgata gtactaagag aagtgcttca agtgcaaatg ggagcttgtt
     5761 ttcgggccaa tcacagtttg ggcccgggcc cgcggaggag aacaagaaca agaacaagaa
     5821 aaggtcacct gcatcaagag gaagcaacga tgaaggaatg ctttcatttg tttcgggtgt
     5881 gattttgcca agttcaaaca cggggaagtc tggtggaggt ggcgattcgg atcaatcaga
     5941 tctcgaggct tcggtggtga aggaagcgga tagtagtaga gttgtagacc cggagaagaa
     6001 gccgaggaaa cgagggagga aaccggctaa cgggagagag gagccattga atcatgtgga
     6061 ggcagagagg caaaggaggg agaaattaaa tcaaagattc tatgcactta gagcagttgt
     6121 accaaatgtg tcaaaaatgg ataaagcatc acttcttggt gatgcaattg catttatcaa
     6181 tgagttgaaa tcaaaggttc agaattctga ctcagataaa gaggagttga ggaaccaaat
     6241 tgaatcttta aggaatgaat tagccaacaa gggatcaaac tataccggtc ctccaccgtt
     6301 aaatcaagaa ctcaagattg tagatatgga tatcgacgtt aaggtgatcg gatgggatgc
     6361 tatgattcgt atacaatcta ataaaaagaa ccatccagcc gcgaagttaa tggccgctct
     6421 catggaattg gacttagatg tgcaccatgc tagtgtttca gtggtcaacg agttgatgat
     6481 ccaacaagca actgtgaaaa tggggagtcg gctttacacg caagaacaac ttcggatatc
     6541 attgacatct agaattgctg aatcgcgacc cggggaccca gctttcttgt acaaagtggt
     6601 tgataacagc gggttaatta acatctttta cccatacgat gttcctgact atgcgggcta
     6661 tccctatgac gtcccggact atgcaggatc ctatccatat gacgttccag attacgctgc
     6721 tcagtgcagc gcttagagct cgaatttccc cgatcgttca aacatttggc aataaagttt
     6781 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     6841 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     6901 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     6961 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tagcttcatc
     7021 aacgcaagac atgcgcacga ccgtctgaca ggagaggaat ttccgacgag cacagaaagg
     7081 acttgctctt ggacgtaggc ctatttctca ggcacatgta tcaagtgttc ggacgtgggt
     7141 tttcgatggt gtatcagccg ccgccaactg ggagatgagg aggctttctt ggggggcagt
     7201 cagcagttca tttcacaaga cagaggaact tgtaaggaga tgcactgatt tatcttggcg
     7261 caaaccagca ggacgaatta gtgggaatag cccgcgaata tctaagttat gcctgtcggc
     7321 atgagcagaa acttccaatt cgaaacagtt tggagaggtt gtttttgggc ataccttttg
     7381 ttagtcagcc tctcgattgc tcatcgtcat tacacagtac cgaagtttga tcgatctagt
     7441 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
     7501 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
     7561 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
     7621 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
     7681 cgatctgctt cgacgcactc cttctttact ccaccatctc gtccttattg aaaacgtggg
     7741 tagcaccaaa acgaatcaag tcgctggaac tgaagttacc aatcacgctg gatgatttgc
     7801 cagttggatt aatcttgcct ttccccgcat gaataatatt gatgaatgca tgcgtgaggg
     7861 gtatttcgat tttggcaata gctgcaattg ccgcgacatc ctccaacgag cataattctt
     7921 cagaaaaata gcgatgttcc atgttgtcag ggcatgcatg atgcacgtta tgaggtgacg
     7981 gtgctaggca gtattccctc aaagtttcat agtcagtatc atattcatca ttgcattcct
     8041 gcaagagaga attgagacgc aatccacacg ctgcggcaac cttccggcgt tcgtggtcta
     8101 tttgctcttg gacgttgcaa acgtaagtgt tggatcccgg tcggcatcta ctctattcct
     8161 ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc
     8221 catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc
     8281 cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt
     8341 caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccgcg
     8401 gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag
     8461 ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg
     8521 cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc
     8581 cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct
     8641 catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat
     8701 acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc
     8761 cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat
     8821 ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca
     8881 acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aattccccaa
     8941 tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat
     9001 ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat
     9061 cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt
     9121 cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat
     9181 cggggtcgtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg
     9241 atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt
     9301 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
     9361 tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg
     9421 gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg
     9481 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
     9541 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
     9601 catgttgacg gatctctagg acgcgtcgac gcgtcgacgc gtcgacgcgt cgacgcgtcc
     9661 tagaagctaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     9721 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     9781 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgcccgc tcctttcgct
     9841 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9901 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     9961 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    10021 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    10081 tcgggctatt cttttgattt ataagggatt ttgccgattt cggaaccacc atcaaacagg
    10141 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
    10201 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accccagtac
    10261 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
    10321 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
    10381 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
    10441 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
    10501 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
    10561 cctgtgatca aatatcatct ccctcgcaga gatccgaatt atcagccttc ttattcattt
    10621 ctcgcttaac cgtgacaggc tgtcgatctt gagaactatg ccgacataat aggaaatcgc
    10681 tggataaagc cgctgaggaa gctgagtggc gctatttctt tagaagtgaa cgttgacgat
    10741 atcaactccc ctatccattg ctcaccgaat ggtacaggtc ggggacccga agttccgact
    10801 gtcggcctga tgcatccccg gctgatcgac cccagatctg gggctgagaa agcccagtaa
    10861 ggaaacaact gtaggttcga gtcgcgagat cccccggaac caaaggaagt aggttaaacc
    10921 cgctccgatc aggccgagcc acgccaggcc gagaacattg gttcctgtag gcatcgggat
    10981 tggcggatca aacactaaag ctactggaac gagcagaagt cctccggccg ccagttgcca
    11041 ggcggtaaag gtgagcagag gcacgggagg ttgccacttg cgggtcagca cggttccgaa
    11101 cgccatggaa accgcccccg ccaggcccgc tgcgacgccg acaggatcta gcgctgcgtt
    11161 tggtgtcaac accaacagcg ccacgcccgc agttccgcaa atagccccca ggaccgccat
    11221 caatcgtatc gggctaccta gcagagcggc agagatgaac acgaccatca gcggctgcac
    11281 agcgcctacc gtcgccgcga ccccgcccgg caggcggtag accgaaataa acaacaagct
    11341 ccagaatagc gaaatattaa gtgcgccgag gatgaagatg cgcatccacc agattcccgt
    11401 tggaatctgt cggacgatca tcacgagcaa taaacccgcc ggcaacgccc gcagcagcat
    11461 accggcgacc cctcggcctc gctgttcggg ctccacgaaa acgccggaca gatgcgcctt
    11521 gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac agcccaatga tctcgccgtc
    11581 gatgtaggcg ccgaatgcca cggcatctcg caaccgttca gcgaacgcct ccatgggctt
    11641 tttctcctcg tgctcgtaaa cggacccgaa catctctgga gctttcttca gggccgacaa
    11701 tcggatctcg cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct gagccttaat
    11761 cacaattgtc aattttaatc ctctgtttat cggcagttcg tagagcgcgc cgtgcgtccc
    11821 gagcgatact gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg aaatgccagt
    11881 aaagcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa
    11941 tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg
    12001 caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg
    12061 aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac
    12121 atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg
    12181 tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc
    12241 ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg
    12301 cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc
    12361 gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag
    12421 gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt
    12481 tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg
    12541 tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac
    12601 agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata
    12661 tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc
    12721 tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc
    12781 gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg
    12841 agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc
    12901 acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag
    12961 gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa
    13021 aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac
    13081 cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac
    13141 ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag
    13201 accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc
    13261 gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg
    13321 aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta ggtactaaaa
    13381 caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa
    13441 gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca
    13501 gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact
    13561 tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag
    13621 ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg
    13681 agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc
    13741 caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga
    13801 gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc
    13861 ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc
    13921 atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat
    13981 catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt
    14041 taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc
    14101 cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat
    14161 tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
    14221 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
    14281 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
    14341 attttgaaac cacaattatg ggtgatgctg ccaacttact gatttagtgt atgatggtgt
    14401 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
    14461 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
    14521 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
    14581 atcattgata tggccatgaa tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt
    14641 ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta acctcgcgca
    14701 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggctatgtcg
    14761 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga cagtctccgg aagacggttg
    14821 ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg gcgtcttatg agcctgctgt
    14881 caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa tcccgcctga
    14941 agggaaagct gcacgtaatc agcaagcgat atacgcagcg aattgagcgg cataacctga
    15001 atctgaggca gcacctggca cggctgggac ggaagtcgct gtcgttctca aaatcggtgg
    15061 agctgcatga caaagtcatc gggcattatc tgaacataaa acactatcaa taagttggag
    15121 tcattaccca attatgatag aatttacaag ctataaggtt attgtcctgg gtttcaagca
    15181 ttagtccatg caagttttta tgctttgccc attctataga tatattgata agcgcgctgc
    15241 ctatgccttg ccccctgaaa tccttacata cggcgatatc ttctatataa aagatatatt
    15301 atcttatcag tattgtcaat atattcaagg caatctgcct cctcatcctc ttcatcctct
    15361 tcgtcttggt agctttttaa atatggcgct tcatagagta attctgtaaa ggtccaattc
    15421 tcgttttcat acctcggtat aatcttacct atcacctcaa atggttcgct gggtttatcg
    15481 cacccccgaa cacgagcacg gcacccgcga ccactatgcc aagaatgccc aaggtaaaaa
    15541 ttgccggccc cgccatgaag tccgtgaatg ccccgacggc cgaagtgaag ggcaggccgc
    15601 cacccaggcc gccgccctca ctgcccggca cctggtcgct gaatgtcgat gccagcacct
    15661 gcggcacgtc aatgcttccg ggcgtcgcgc tcgggctgat cgcccatccc gttactgccc
    15721 cgatcccggc aatggcaagg actgccagcg ctgccatttt tggggtgagg ccgttcgcgg
    15781 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga
    15841 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta
    15901 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg
    15961 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat
    16021 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc
    16081 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg
    16141 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag
    16201 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg
    16261 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag
    16321 ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc
    16381 gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc
    16441 ccggtgagcg tcggaaaggc gc
//
