LOCUS       sequence-246520-       13304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into 
            a dox-inducible piggybac destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Insco ML, Abraham BJ, Dubbury SJ, Kaltheuner IH, Dust S, Wu C, Chen 
            KY, Liu D, Bellaousov S, Cox AM, Martin BJE, Zhang T, Ludwig CG, 
            Fabo T, Modhurima R, Esgdaille DE, Henriques T, Brown KM, Chanock 
            SJ, Geyer M, Adelman K, Sharp PA, Young RA, Boutz PL, Zon LI
  TITLE     Oncogenic CDK13 mutations impede nuclear RNA surveillance.
  JOURNAL   Science. 2023 Apr 21;380(6642):eabn7625. doi: 
            10.1126/science.abn7625. Epub 2023 Apr 21.
  PUBMED    37079685
REFERENCE   2  (bases 1 to 13304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-04-21"; volume: "380(6642):eabn7625. doi"; pages: " 
            10.1126/science.abn7625. Epub 2023 Apr 21"
COMMENT     Sequence Label: PB-Neo-TetO-Cdk13_Untagged
FEATURES             Location/Qualifiers
     source          1..13304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   746..808
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1021..1245
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(1305..1346)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    1348..1372
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2395..2412)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     protein_bind    complement(5963..5987)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6099..6123)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6120..6158)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(6191..6209,6210..6232,6233..6251,6252..6274,6275..6293,
                     6294..6316,6317..6335,6336..6358,6359..6377,6378..6400,
                     6401..6419,6420..6442,6443..6461)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     polyA_signal    6564..6675
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(6713..7516)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6824..6843)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7434..7453
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(7578..7597)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7971..7994
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        complement(8020..8229)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8025..8160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8030..8327)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(8079..8098)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8368..8384
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8369..8385
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8734..8768
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9261..9277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9261..9277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9274..9296)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9285..9301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9309..9315,9316..9333,9334..9339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9354..9375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9492..9509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9616..10119
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     10160..10178
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10216..10238)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10338..10357
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10381..10596
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10597..11400
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(11557..12145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11637..11656)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12316..13107,13108..13176))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12939..12958
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13177..13281)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gtgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa acaaaaactc aaaatttctt
      961 ctataaagta acaaaacttt tatcgaattc ctgcagccca gcttgatccc gcctcagaag
     1021 ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc
     1081 tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa
     1141 tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca
     1201 cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag gctgatcagc
     1261 gggtttaaac gggccctcta gaggttagcg ctagctcatt actacgtaga atcgagaccg
     1321 aggagagggt tagggatagg cttaccaacc actttgtaca agaaagctgg gtcgaattcg
     1381 cccttctcga gtcagtatgg taaccctctg cctcgacctc tgccagctga tgtgctaatg
     1441 tgtcccctgt atccttgtga atatccaggt ccttgggcag gagaagtcca ggtctgtcca
     1501 tgcatgaggc tagggccacc tggattttcc tgtaagttac caccatagtt atagttgtgc
     1561 atctttgctg gcaagggatg agtactctct ggccctgtgg aagggtcatt gctgtttgtg
     1621 aggactgtga taggaccatg gctatattca aatctatggt ccttgtctcc actaaacaac
     1681 atgggaccag cattgaggta aatgtctcca tatccagcta ctgaactggc aaacgactca
     1741 gtaaaggcag aagtttgagg tggaccacca gtgtgagatg aagcagtact gtagttatcc
     1801 agagactgaa tatctgagtt tccaatgaaa ctacgtgctt ccaatggtgc agcagcggag
     1861 ctacttccta gaccatcact gctaacgtaa ggagcggcag agaaagaaga tccaaagttg
     1921 tccttatagt ctgaactggg cacatatgtg tctcctgtgg gataatcgat accaccttct
     1981 cttttgggat catcctgggg ctgatgctga gcaagcagct gtaagagggc tgccttcact
     2041 ccagcatgtg ggtcagttaa tgaagaacta gtggtaggaa catgctggtt ttctgtccga
     2101 taatctaggt cctcctcagt aactggtggt ggttcaggag gttcaggagg ccgttgatca
     2161 ggaggcagaa ttcgaggccg gtctggttct ggtgtcagct ccaatatcct cctgtcttgg
     2221 tgctggatga ggtcatcttg cccggatcca ggagtgctcg gttctagagg aggcctgagt
     2281 tgcaatggag cttcatgtcc tgatccattt tccctttcct ctagcatggc atctttctgt
     2341 ttggactgtt gggcctttat taactgagtc aagaggactg caaatgcact ctgcacagca
     2401 gcctgggcag catcagtttc cactttaggc tggatggtct gtgaaggctg aactcctccc
     2461 aatgattttg aagactcctg ttgtggtgtt gatggatctg tctgtttttc acctgttgcc
     2521 aaaattccag caggaaggtt tattttattt agctgctgtt gagtctcaga gtttacctta
     2581 atgttcaaca cttggacaaa atcagccata ttaacacttg atttagattg tagtaggttt
     2641 agaagaattg ccaattcact gtggtttaac ggctgtccag ggcctgttat tacaggtgct
     2701 acatttgagt tgctctgaga tttcaactgt gcgggtggca gcacaccctg gggtgtgtta
     2761 gttctgctgt catctaagcc cagagacagg tccttcctag gggctttgat tgtggaaaga
     2821 tcatcagtca tgcccatctg tttctgtctt cttctctttt tactccataa ttcatgacaa
     2881 tcttgccaca aaggaaggtc tggtggaggc attttggagg gttccacgtc tcgcaggaac
     2941 tcacactgaa gagcctgctc agcagtgcag cgcttactgg gatccaaggc aagcatgtaa
     3001 tcgaataagt cgagtgcagc tgcggggatg aaaacaaatt cttctcttaa cttccgccga
     3061 tattgcttct ttggtttcat ggtgttgaaa tatggcagtt tgattacatc aggccacact
     3121 gcaggacatg gactcccaca tatacggctt attagctcta gctgtgcaag ttcctggttt
     3181 gcttgaaata taggtttttt agtgaagagt tcaccaagga tacatccaca gctccataca
     3241 tcaatggctg gtgtatatcg ttcttctccc aagagcaatt caggtggacg ataccacaaa
     3301 gtaatgacct tgttagtata tgggcgactt tcttctgagc tatataaacg agcaagtcca
     3361 aaatctgcaa gttttatctg tcctctatta tttagaagga tatttgaaca tttaatatct
     3421 ctatgcaaaa agttcttctt atgacaataa tccaggcctt ccatgagctg tctcataaaa
     3481 gattttatat ggttttcatt aaaatgaacc aagcctgatt ccagcagtcc catcagatca
     3541 tggtccatat attcaaacac caggtaaaat gcacctttgt ctttcttaaa atccaaagca
     3601 tcttctttat cagtcacgat ttccttcata ttgatgatac tctggtgggt gagttgccga
     3661 agaattttaa tttctctaat tgctgtaatt gggaaaccct ccttttcatt atccagacgt
     3721 actttcttta aggctaccat ttctcccgtg tctttgtccc tggctttgta aacttgtcca
     3781 taagtacctt ctccaataat tccgatgata tcaaatttat ccacgcagcg tttcccccag
     3841 tcaatatctt tttctttgat ttcaccatag cgaggcccac atattttagg ccttcgtttg
     3901 ctatgtaact gtgctgctgt tttcttctcc tctggactct tggaaagatc atctcctcct
     3961 ggtaactcag ggggcaatgg taaatcagca agcagacatc ggagtttctt ttctacttct
     4021 tttttaactg ctttgacaga aatgttgcct cttaagctat cagcatcttt atcttcaggc
     4081 agcatgggag gcaaaggtaa tggcggtaac gtagaggtca ctaaagccac atgctgctcc
     4141 ttctccttgg ctcctgtgct tggtgtgagt ggcttgcttt tctctttagt ggaaactggt
     4201 tcttcctttg tagcagcagg tttactctcc ttcccaacaa ctgttttctt ggtggctttc
     4261 tctactgtca aattattgtc tacctttgtt acttgaagcg gtggctttgt ttttgctttg
     4321 tcgcttttga cggtcccacc acttgaagga gaaggtgcat gctcagtttt aagcttcttg
     4381 acttccttca cgtggttggt ctgggagacg gaggctccgg tctcggtgtt tcccttggtg
     4441 ggtgtggaag cgttggaggc cttagcagcc ttggcggcgg cttcagcagc tttggcggct
     4501 tcggcggctt tagcggcttc ggcagctcta gcagcctcag cggccctagc tttcttgttc
     4561 ttgttgagct cggcggccag ggagctcttc agtgtcaggg tggaggggct gatggaggag
     4621 tgcctgcttc tggacctgct cagcctgtgc ctggacctgc ttctgctgtg cctggaggag
     4681 taagggctcc tggacctgga cttggcggac ctcctcagca caggggaata gggggatctg
     4741 ctcctcctcc aggaggagga gctgtagggg gagggggaga catctcctcc tctctcgtag
     4801 gaggaatgcc tgctgtagct gggggatctc ctcctgctgt aggggctagg ggacctgggc
     4861 agtcttctgg aataagggct gcttccgcct ccagcggggg aagggctttt cctggatctc
     4921 agggactggc tagccctgtg gctgacaggg gactcgtctc tgcctccgag agggctgagg
     4981 gacctctgcc tcctcctgta ggccttgggt tcggacttgt cttccctgta ggctttgggg
     5041 ggctccttgt aggcggaagg gggttccttg gagctcttgg tcctgctcct atgagccttg
     5101 aggtccctgt ccttcctgga gctgctgctg gaggatgtgg cggaggcgga ctttcttctt
     5161 ccgccggagg agctggagga gccggatttg gcagcctcgg ctctttcctc gccgctgtgt
     5221 ccgtgtctgg atctggcctt gctggcctcg gatccgctcc ttgtgccatc tctcctccta
     5281 tgttccctgt gcctctcttt ggacctgccg ctggagctcc ttctgtccct tctaggtctt
     5341 ctctcgctgc cctcggctct tctctgggtt ccgctgctgg aagcggggga tcctccattt
     5401 cctccggttc ctccagcagc ggtggcagct gtagcagcgc tagctcctcc caggagcagg
     5461 ccttgttcgc tttggctgga cacgtcctcg tactccacca ggggtgtcac tcctcctccg
     5521 ctggaagctc ctccgcctcc atcctgctgg ggttgaggca gggagaacac tctcctcttt
     5581 tcggcttcct gtccggccct aggtcctctc cttctctttt gcctgccgcc gggtctcctt
     5641 tttcctctgg ccagcctctt gacctccaga ggagggccag ggctaaaaca ggagctggaa
     5701 gcggcagcag cagcggcggc agcggcagcg gctccaggag cagccagaaa caggagagga
     5761 gggggaggag gagggggttg gaggagctga ggttgcagga gagggagcag cagaggaggc
     5821 tgctgagggc tgagaaacct cctcctcttc ctcctctcct ccagcttttt ctcggcccag
     5881 ctgagtccgc ctcctccgcc gagagctgta tcggagctgg aaggcatggt ggcggcggta
     5941 ccagtagtaa gggcgaattc ggagcctgct tttttgtaca aacttgttga tatctgcact
     6001 agtggatccg agctcggtac gaattcgggg ccgcggaggc tggatcggtc ccggtgtctt
     6061 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
     6121 gctctgctta tataggcctc ccaccgtaca cgcctacctc gacccgggta ccgagctcga
     6181 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
     6241 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     6301 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     6361 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
     6421 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgagttgcc
     6481 tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag
     6541 aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg
     6601 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
     6661 aactagaagg cacagtcgag gctgatcagc gagctctaga gaattgatcc cctcagaaga
     6721 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
     6781 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
     6841 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
     6901 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
     6961 catcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
     7021 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
     7081 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
     7141 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
     7201 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
     7261 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
     7321 cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
     7381 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
     7441 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
     7501 caatggccga tcccatattg gctgcaggtc gaaaggcccg gagatgagga agaggagaac
     7561 agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga ggaccttcgg
     7621 gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc ccttcccagc
     7681 ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca aaggcctacc
     7741 cgcttccatt gctcagcggt gctgtccatc tgcacgagac tagtgagacg tgctacttcc
     7801 atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct gactagggga
     7861 ggagtagaag gtggcgcgaa ggggccacca aagaacggag ccggttggcg cctaccggtg
     7921 gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc cagcggggct
     7981 gctaaagcgc atgctccaga ctgccttggg aaaagtactg cctaggcctc caaaaaagcc
     8041 tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg cataaataaa
     8101 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     8161 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     8221 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     8281 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     8341 acagcagaga tccagtttgg ttagatctcg aggtcgacgg tatcgttggc cggtactcga
     8401 cgtagggata agatcggaag agcggttcag caggaatgcc gagaccgcgc caacgataag
     8461 ctatctataa caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa
     8521 caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat
     8581 tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg
     8641 cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc
     8701 tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct
     8761 ttctagggtt aatctagctg catcaggatc atatcgtcgg gtcttttttc aattgctcag
     8821 tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg ccttttcccg
     8881 cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga
     8941 gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg
     9001 tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc ggacacagtt
     9061 ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg
     9121 ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg tcagcgagga
     9181 cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt tacccggcgg
     9241 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9301 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9361 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9421 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     9481 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9541 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9601 acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa
     9661 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
     9721 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt
     9781 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg
     9841 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
     9901 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
     9961 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
    10021 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
    10081 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtctaagaaa ccattattat
    10141 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    10201 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    10261 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    10321 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc ataccacctt
    10381 ttcaattcat catttttttt ttattctttt ttttgatttc ggtttccttg aaattttttt
    10441 gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta
    10501 tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg
    10561 cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa
    10621 cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag
    10681 caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt
    10741 gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt
    10801 tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc
    10861 ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt
    10921 gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt
    10981 attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg
    11041 atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact
    11101 gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg
    11161 ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac
    11221 aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct
    11281 gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt
    11341 gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa
    11401 aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa
    11461 ttatatcagt tattacccta tgcggtgtga aataccgcac agagcaaaag gccagcaaaa
    11521 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    11581 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11641 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    11701 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    11761 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    11821 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    11881 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    11941 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    12001 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    12061 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    12121 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    12181 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    12241 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    12301 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    12361 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    12421 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    12481 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    12541 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    12601 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    12661 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    12721 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    12781 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    12841 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    12901 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    12961 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    13021 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    13081 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    13141 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    13201 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    13261 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
