LOCUS       sequence-246521-        7049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human codon-optimized D. melanogaster Orco (hOrco) and Or56a 
            (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin 
            (mRho) and HCN1 (mER) for improved trafficking in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, 
            Lavista-Llanos S
  TITLE     Optimization of Insect Odorant Receptor Trafficking and Functional 
            Expression Via Transient Transfection in HEK293 Cells.
  JOURNAL   Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062.
  PUBMED    31504297
REFERENCE   2  (bases 1 to 7049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Senses"; date: "2019-10-26- 26"; volume: "44"; issue: "9"; 
            pages: "673-682"
COMMENT     Sequence Label: pDmelOR-mRho.V5.mER.hOr56a
FEATURES             Location/Qualifiers
     source          1..7049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     112..133
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(337..356)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     456..478
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(516..534)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        602..706
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(707..775,776..1567)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(925..944)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1738..2326
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2227..2246
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2615..2696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4129..4170)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      4188..4197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        4339..4642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4643..4846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4797..4817
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4880..4889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4889..4918
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          6134..6266
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     6249..6268
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    6647..6728
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6739..6756)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      join(6864..7049,1..270)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6951..6970)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
       61 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      121 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
      181 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
      241 attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
      301 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
      361 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
      421 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
      481 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
      541 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      601 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      661 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      721 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      781 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      841 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      901 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      961 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1021 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1081 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1141 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1201 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1261 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1321 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1381 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1441 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1501 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1561 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1621 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1681 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1741 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1801 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1861 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1921 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1981 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2041 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2101 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2161 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2221 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2281 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2341 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatggctc gacagatctt
     2401 caatattggc cattagccat attattcatt ggttatatag cataaatcaa tattggctat
     2461 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattta tcagggttat
     2521 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2581 cgcacatttc cccgaaaagt gccacctgac gtcggcagtg aaaaaaatgc tttatttgtg
     2641 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     2701 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     2761 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagac tgcagcctca
     2821 ggagatctgg gcccccgcgg catatgaccg gtgtcagtac agatgagaac tcctcaaaag
     2881 attgaagtat gagtacgcac ctcgtcctat gtcaatgaac gttcttagat ttaggtcaac
     2941 aagcagtgcc ctcattttca tcggccgctg cgcatgcatc atcatgaata ctaacatctt
     3001 ctggagaggc atctcgaagt tataccatcc acacgagaaa tacgcaaggc ttaactcgtc
     3061 gtgacactca acgatgagtg tagcgtgcca atggtatatc cagagtatcc ctgccattac
     3121 gaaaagatat atgaacatga agaagtagtc cattttagac ggtactccga ctgtgagagc
     3181 gaagaaaagg aaacatatca gcaccgagaa tatgataaag tcggccatta cgggaacgca
     3241 gatgaggtat tgcagttctt gaacgaactt acgtatcctc aactgctcct taacgaactc
     3301 cttaaacagt tgcatttgcc agaaggttaa ctcacttgcc gtcaatcgtc ctattacaag
     3361 tttactgttc agacgtgtta tgtccatctc ctcaacacgt ttgttaagga tttgcagctt
     3421 tagattaacg tatttcataa gacaagttat aaatgtatgc cagagtggaa tagcagtaat
     3481 ccccaggccg agtgcgtacc acgggcctaa ataccctacg acaaagttat cacacaactc
     3541 cccaaatgga aacaactgga acaacaagat gggatgttcc tcgccgcgac gcacagctgg
     3601 cacgttgaaa acagactttg ggagaaataa tgaacgataa atacagtccg aatatgccat
     3661 taggccagcg attactgagg gtatccatat gagtaaagtt atcgtccgag tgtatgcttg
     3721 tgttgccact aaaagctcca tctctctatt atccgcttca tgcattaggt tctgtatgtc
     3781 ggaatgcgcg acacgaagaa gtgagatgac cttgtcacgt tgcacgtaag cgttgaacat
     3841 ggctattgac accgcgaaat attgcacagc tgtcgcgtaa gacgtcgcac cgtcattcaa
     3901 attttcatag cctctgaaaa cgcgggcgag cattagtgcg cagcttagcc aaatagatgc
     3961 cgttaaaatc gtacagcgga tgagactgag caacgggcga ttttgatcct tcgaggcgac
     4021 atacccatac cattgaaaat agcggagatg ggtgccgaag atcgggtcct cgaatgtcgt
     4081 aggagagagc aacaagtcct ttaccttgaa cagactaaac ttgttaacgg tgctatcaag
     4141 ccccagaaga ggatttggga tgggttttcc tgctggagcg acttgatcca tggtggcctc
     4201 caggcgatct gacggttcac taaacgagct ctgcttatat aggcctccca ccgtacacgc
     4261 cacctcgaca tactcgagta gttattaata gtaatcaatt acggggtcat tagttcatag
     4321 cccatatatg gagttccgcg ttacataact tacggtaaat ggctcgcctg gctgaccgcc
     4381 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     4441 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4501 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     4561 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     4621 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     4681 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     4741 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     4801 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
     4861 tcagatccgc tagggatccg ccaccatgga acagaaactg atctctgaag aagacctggc
     4921 tagcacaact agcatgcaac ccagtaaata tacaggcctg gtggccgatc tcatgcccaa
     4981 cataagggca atgaaataca gcgggctctt tatgcacaac tttacgggag ggtccgcctt
     5041 catgaagaaa gtctacagca gcgtacatct cgttttcctt ctgatgcagt tcaccttcat
     5101 tctggtcaat atggccctta atgccgaaga ggtgaatgag ctttctggga ataccatcac
     5161 cacactgttc ttcacacact gcatcacaaa gttcatctac ctggcagtga accagaagaa
     5221 cttttaccgt acactgaaca tctggaatca agtgaacacc caccctctgt ttgccgaatc
     5281 agatgccaga taccacagta ttgcactcgc caagatgagg aaactcttct ttcttgtgat
     5341 gctgacaaca gtggctagtg ctactgcctg gaccactatc acgttctttg gcgattctgt
     5401 gaaaatggtg gtagaccatg aaaccaacag cagcatccct gtcgagattc caagacttcc
     5461 gattaagtcc ttttatccct ggaatgcaag tcatggcatg ttctacatga tctccttcgc
     5521 cttccaaatc tactacgtgc tgtttagcat gatacacagc aacctctgtg acgtgatgtt
     5581 ctgctcttgg ctgatatttg cctgtgaaca gctccagcat ctgaaaggca ttatgaagcc
     5641 actgatggaa ctgtcagcct cacttgacac ttatcgccca aattctgctg ctctctttag
     5701 gtcactctca gctaatagca aatccgagtt gattcacaac gaggagaaag accctgggac
     5761 tgacatggac atgtctggca tttatagcag caaagccgac tggggagcac agtttcgggc
     5821 accttctaca ctccagtcct ttggcggtaa tggaggagga ggtaatgggc tggttaacgg
     5881 tgctaacccc aacggtctga ccaagaaaca ggagatgatg gtgcgatctg cgatcaagta
     5941 ctgggtcgaa cgccataagc acgtggttag actggttgct gctataggcg atacctacgg
     6001 tgccgctttg ctgttgcata tgctgactag cacgatcaag ttgactctgt tggcatatca
     6061 agccaccaag atcaatggag tgaacgtata tgcgtttacg gttgtggggt atctgggcta
     6121 tgctctggcc caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact
     6181 gggcttgtcg agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga
     6241 catccacttt gcctttctct ccacaggtgt ttcacttttg cattttcggc aatcggctga
     6301 tagaggagag ttcctctgtt atggaggcag cgtacagttg ccactggtac gatggaagcg
     6361 aagaagccaa gactttcgtt cagattgtgt gtcagcagtg tcagaaggct atgtccatct
     6421 caggggcgaa attcttcacc gtgtccttgg atctgtttgc ctccgtactg ggcgcagtcg
     6481 tcacctattt catggtcctg gtccaactga agtgaaagct tgtcgacgat atctccagag
     6541 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     6601 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     6661 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6721 ttcactgccc cgagcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6781 agcggtatca gctcactcaa aggcggtaat acggttatcc agatcgccct tcccaacagt
     6841 tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6901 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6961 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7021 gctcccttta gggttccgat ttagtgctt
//
