LOCUS       sequence-246525-       13223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into 
            a dox-inducible piggybac destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Dubbury SJ, Boutz PL, Sharp PA
  TITLE     CDK12 regulates DNA repair genes by suppressing intronic 
            polyadenylation.
  JOURNAL   Nature. 2018 Dec;564(7734):141-145. doi: 10.1038/s41586-018-0758-y. 
            Epub 2018 Nov 28.
  PUBMED    30487607
REFERENCE   2  (bases 1 to 13223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0758-y"; journalName: "Nature"; date: "2018-12";
            volume: "564"; issue: "7734"; pages: "141-145"
COMMENT     Sequence Label: PB-Neo-TetO-Cdk12_Untagged
FEATURES             Location/Qualifiers
     source          1..13223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   746..808
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1021..1245
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(1305..1346)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    1348..1372
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5882..5906)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6018..6042)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6039..6077)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(6110..6128,6129..6151,6152..6170,6171..6193,6194..6212,
                     6213..6235,6236..6254,6255..6277,6278..6296,6297..6319,
                     6320..6338,6339..6361,6362..6380)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     polyA_signal    6483..6594
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(6632..7435)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6743..6762)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7353..7372
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(7497..7516)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7890..7913
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        complement(7939..8148)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7944..8079
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7949..8246)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(7998..8017)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8287..8303
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8288..8304
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8653..8687
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9180..9196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9180..9196)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9193..9215)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9204..9220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9228..9234,9235..9252,9253..9258))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9273..9294
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9411..9428)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9535..10038
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     10079..10097
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10135..10157)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10257..10276
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10300..10515
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10516..11319
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(11476..12064)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11556..11575)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12235..13026,13027..13095))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12858..12877
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13096..13200)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gtgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa acaaaaactc aaaatttctt
      961 ctataaagta acaaaacttt tatcgaattc ctgcagccca gcttgatccc gcctcagaag
     1021 ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc
     1081 tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa
     1141 tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca
     1201 cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag gctgatcagc
     1261 gggtttaaac gggccctcta gaggttagcg ctagctcatt actacgtaga atcgagaccg
     1321 aggagagggt tagggatagg cttaccaacc actttgtaca agaaagctgg gtcgaattcg
     1381 cccttctcga gttaataagg aactcctctc cctcttcccc ctcgtggtag gactcttgca
     1441 gcccccctgt agggttttcc ataagactga gctgggcccc cccactgaag cgttgttcct
     1501 gagctgttgg ccccagtggt tcccggtccc agctccccat agttttgcaa tttggtctct
     1561 gcatgtacca cagagttgct atttccctca gacttgagga atggttgccc aaccactgag
     1621 tgtaatgcta agggaaccct ggtaaaacgg aggtcctggt cccctgggaa ctgcactgac
     1681 cctgtgccct ggtagctgtt ggactcccca aggtggctca cagagcctga gaaggtcctg
     1741 ttcttcaccg gggtctgaac caaagattct gtcaaggctg gaccagaact gcgttcatca
     1801 gtgtcagcgg aactgaaccc tgtctcaggc ccatcagtat ttccgtaagt cctgtttgga
     1861 tgggatcggg tggagtattc cattggtctc aaggcctggt gctcatgtgg caggtggcca
     1921 ggaggctctg cttcaggctg ggataaaagt tgcagcaagg cggctgtcac ggcgggattc
     1981 aactcctggg gggcgctgga aagatcgcct tcaaccagag ggggtggagg tggcggcggt
     2041 ggaggtccag ggggctcagg gggcctcttc tctggtggaa gaatgtgagg aggacatgct
     2101 gctgcctcct cctgtggcat tgtaggagtt cttcggggcc cctgtggccc actattcttg
     2161 tcattggtgt tttcctccag gttacctgct ggctcttggg ttttcatcag ctgactcaag
     2221 agaactgcca acacattctg catgtcagct ggtgtgtttg aagcttcagg agttgtctgt
     2281 tcagcaggag gcaggaccac aggtacagaa ggtacctcct tcaatgattc ttcaggggct
     2341 atggattctg agtcctgctg ctgggaactg gcttcagtca gtgcactaat agactgattc
     2401 aaggcttcaa gctgctgttg catctctgga ttggagtgga tattaagcag ctgtgccatc
     2461 tgagggatgc tcaggtcagt ttggctctga agcaggttta ataacactgc caattcactt
     2521 tgattcaact gctgtgtgat gtcaccaagg cctactgcat cccctggacc aggctctgcc
     2581 ttgacaggag caggctgagg tggtgctggg ctattgtttt tcacaggctc agctgttgtc
     2641 cctgaggtag tttcttttct agaagctttg gacggaggtg gatcttctat cacaatacca
     2701 ctctgtcgct gccgtcgacg tttcttactc cacaattcat ggcaatcctg ccagtgaggt
     2761 aggtctggag gtgccatttt gctgagttcc acatctttaa gaaagtcact ctgtagggtc
     2821 tgttcagctg tgcacctctt gctaggatcc agtgtcagca tgtggtccaa tagatcaagt
     2881 gccgctgaag gaatgaaaga gaattcttct cttaggcgtc tcctgtattg cttcttcggt
     2941 ttcatggtgt tgaagtaggg cagcttgata acatcaggcc acactgctgg acaaggacta
     3001 ccacagagac gactgataag ttctagctga gccagttcta aattggcttg aaaaataggt
     3061 ttctttgtga acagttctcc aaggatgcac ccacagctcc aaacatcaat ggctggtgtg
     3121 tatctttcct ctccaagaag aagctctgga ggtcgatacc aaagagtgat gactttgttt
     3181 gtgtaagggc gactctcttc agagttatag agccgagcaa gtccaaaatc tgccagtttg
     3241 atttgcccgc tgttattcag caaaatgtta gaacatttaa tatcccgatg gaggaaattc
     3301 tttttgtgac agtaatccag tccttccatt agctgtttca taaatgactt gatatggtcc
     3361 tcagaaaagt gcaccaaacc tgattcaagc agtcccatta agtcatggtc catatattca
     3421 aatacaaggt aaaaggcacc tttgtccttc ttgaaatcca gtgcatcttg tttgtctgtg
     3481 acaatttcct tcatgtttac aacactctgg tgaactaatt gacgaagaat cttgatctcc
     3541 cggatggctg tgattgggaa gccctctttc tcattgtcca gccgaacctt cttcagagct
     3601 actagttctc ctgtgtcttt gtccttggct ttatatactt ggccataggt cccctctcca
     3661 ataatcccaa taatgtcaaa cttgtccaca cagcgcttcc cccaatcact ttctgtttgt
     3721 cttctttctc cataacgtgg acaacaaatt tttggtctct ttttatatgg ttgttgaggt
     3781 ggtgtaattg cctttggctc tggagaatct ggaggcgatg gatctcctcc tggtagctca
     3841 ggaggaagag gcaagtctgt aagcaagtgg cgagtcctct gttccttctc tttctttgca
     3901 ggctttgaag gaagtgtttc ttttggacta tccatgtcat catctccagg taatagggga
     3961 gggaggggca gaggaggcaa tgttgaagtc ttcagatgtg gaatagcagc tgttatagat
     4021 acttgagtct tcatagaaac ctgtacaggg ggctgagaat ttgtctgaga ggatagagta
     4081 gatgtccttg ggtgaggaga agagggtaaa attgaagtac ttgaaacagg aacttgacta
     4141 aatggtggtt ggggaggagg cagaggtggc tgcagcggaa tagctggtag tggaggcaaa
     4201 ggtggcaaag ggggtgtctg aggtggaggg gtagtagctg gtaaaggggg tggagaagta
     4261 attgtgggaa gaggtggaag ggtctccttt tctgatgtct ctgtctcctt tgaagtaaca
     4321 atcacctctt tcggtgctac aggtttaacg tcctttgttc cctgtgcttt caaatcctta
     4381 gcaggatgct tctgcaagtt ctcatccaac ctaaccttcc ctgtgtctaa acaatgtttt
     4441 acctctgggt ttgaatgtgc tacagtaacc agttcagtat ctggggtcga tttttctgat
     4501 tttatacttc tgggtaactt tttagactct aaccctgact ccttcacctc aagtttctct
     4561 tttttaggca aaattatagg tgaactcttg gactcttttc catccatttt tgctgctgca
     4621 gcagctgctg ctctttcctt cttttttcta ctgagttcag ctcccaggct ggaattcaat
     4681 ggaagcctga caggtgagat actggaatga cgactgcgtg acccggatcg cttcttttta
     4741 ctatgagaag atgagtgtct tgaatatgca ggacttctac ttctggactt catggatttc
     4801 ctactgggga gtggactccg actcagagac ctcttgctca ggaaagggct gcttgatcgc
     4861 cttcggccat aggggctggg tgatctcccg ctgtaagagc cgctcctttc atagctggag
     4921 gaccgtctcc ggctataggg actcacagac ctctgtcgtc ggctgtaagg actgggtgac
     4981 cgagtgctgg actggtaagc agaaggctct ttgtaaggtg ggctgattga ctgccttctt
     5041 gaggtacttc caggactctt cttgtaggag tcatagttgc tagaagtgtg agaccttggg
     5101 gggctaagat cgtagtcttg gccataagaa gctccggaag ggctgtcatc ttgcttggaa
     5161 ctgtcagacc atttcctatg gggactcctg gatctccgtt tagggctagc cacggttttg
     5221 taacttttgg gtgtttccct tttccgatga cttttactcc ggtccttgta tccagacttt
     5281 aactctcgct ctttgcgggt cttctccttg tgcattttgg acgacctgga ttccttgctg
     5341 ccgcttttgg atagctgggc cttcccataa tcatcactcc cctccacgga ccgtttggaa
     5401 ctgcccgata cccggtcctt catagatcca gatttggaga cttcctgatt tttttccttt
     5461 tccgtctgtt tagtttttag caagtctcgg gaccgccggt gctggtggtg acgatgtcgg
     5521 tgcaggcgat cgctccgatc cgttccccga cgttcctcgt tctccctccg gtctgatttg
     5581 aaggccgtgt catcggagaa ggtgtctgaa tcagagctga tgtcatcgta ctccaccagt
     5641 ggtttgatta tggtacccaa agatgccgct tcgggggtca ccagccccac gtctttggag
     5701 tgcttggact tatgccgctt gtgcttcgac accaagcggt gacgctccct gctgttggag
     5761 ctgccacctc ccgatgacgg ctgcgaagtt ccagaagctc ctccgctccc gtccttcttg
     5821 cccccatgtc tctcggaatt gggcatggtg gcggcgagct cagtagtaag ggcgaattcg
     5881 gagcctgctt ttttgtacaa acttgttgat atctgcacta gtggatccga gctcggtacg
     5941 aattcggggc cgcggaggct ggatcggtcc cggtgtcttc tatggaggtc aaaacagcgt
     6001 ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctctgcttat ataggcctcc
     6061 caccgtacac gcctacctcg acccgggtac cgagctcgac tttcactttt ctctatcact
     6121 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
     6181 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
     6241 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
     6301 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
     6361 ttctctatca ctgataggga gtggtaaact cgagttgcct gctattgtct tcccaatcct
     6421 cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat
     6481 gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca
     6541 aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg
     6601 ctgatcagcg agctctagag aattgatccc ctcagaagaa ctcgtcaaga aggcgataga
     6661 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     6721 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     6781 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     6841 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc atcgccgtcg ggcatgcgcg
     6901 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     6961 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     7021 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     7081 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     7141 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     7201 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg
     7261 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     7321 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     7381 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatggccgat cccatattgg
     7441 ctgcaggtcg aaaggcccgg agatgaggaa gaggagaaca gcgcggcaga cgtgcgcttt
     7501 tgaagcgtgc agaatgccgg gcctccggag gaccttcggg cgcccgcccc gcccctgagc
     7561 ccgcccctga gcccgccccc ggacccaccc cttcccagcc tctgagccca gaaagcgaag
     7621 gagcaaagct gctattggcc gctgccccaa aggcctaccc gcttccattg ctcagcggtg
     7681 ctgtccatct gcacgagact agtgagacgt gctacttcca tttgtcacgt cctgcacgac
     7741 gcgagctgcg gggcgggggg gaacttcctg actaggggag gagtagaagg tggcgcgaag
     7801 gggccaccaa agaacggagc cggttggcgc ctaccggtgg atgtggaatg tgtgcgaggc
     7861 cagaggccac ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca tgctccagac
     7921 tgccttggga aaagtactgc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     7981 ctcagaggca gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     8041 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     8101 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     8161 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     8221 tggggagcct ggggactttc cacaccctaa ctgacacaca cagcagagat ccagtttggt
     8281 tagatctcga ggtcgacggt atcgttggcc ggtactcgac gtagggataa gatcggaaga
     8341 gcggttcagc aggaatgccg agaccgcgcc aacgataagc tatctataac aagaaaatat
     8401 atatataata agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt
     8461 taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat
     8521 agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg
     8581 cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat
     8641 atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta atctagctgc
     8701 atcaggatca tatcgtcggg tcttttttca attgctcagt catcgcccaa gctggcgcta
     8761 tctgggcatc ggggaggaag aagcccgtgc cttttcccgc gaggttgaag cggcatggaa
     8821 agagtttgcc gaggatgact gctgctgcat tgacgttgag cgaaaacgca cgtttaccat
     8881 gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg ttcaggccac
     8941 ctgggatacc agttcgtcgc ggcttttccg gacacagttc cggatggtca gcccgaagcg
     9001 catcagcaac ccgaacaata ccggcgacag ccggaactgc cgtgccggtg tgcagattaa
     9061 tgacagcggt gcggcgctgg gatattacgt cagcgaggac gggtatcctg gctggatgcc
     9121 gcagaaatgg acatggatac cccgtgagtt acccggcggg cgcgcttggc gtaatcatgg
     9181 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9241 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9301 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9361 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9421 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9481 atacggttat ccacagaatc aggggataac gcaggaaaga cctgggtcct tttcatcacg
     9541 tgctataaaa ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa
     9601 agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta
     9661 gggggatcgc caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc
     9721 cgaattgttt catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt
     9781 ttacttatcg ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata
     9841 tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttcttcat
     9901 tccgtaactc ttctaccttc tttatttact ttctaaaatc caaatacaaa acataaaaat
     9961 aaataaacac agagtaaatt cccaaattat tccatcatta aaagatacga ggcgcgtgta
    10021 agttacaggc aagcgatccg tctaagaaac cattattatc atgacattaa cctataaaaa
    10081 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    10141 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    10201 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    10261 atcagagcag attgtactga gagtgcacca taccaccttt tcaattcatc attttttttt
    10321 tattcttttt tttgatttcg gtttccttga aatttttttg attcggtaat ctccgaacag
    10381 aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt
    10441 gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg
    10501 aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt
    10561 cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca
    10621 ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt
    10681 tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag
    10741 ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct
    10801 gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg
    10861 gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag
    10921 gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc
    10981 aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac
    11041 aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac
    11101 gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa
    11161 cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga
    11221 ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc
    11281 tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat
    11341 gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt attaccctat
    11401 gcggtgtgaa ataccgcaca gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    11461 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    11521 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    11581 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    11641 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    11701 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    11761 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    11821 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    11881 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    11941 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    12001 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    12061 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    12121 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    12181 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    12241 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    12301 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    12361 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    12421 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    12481 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    12541 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    12601 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    12661 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    12721 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    12781 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    12841 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    12901 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    12961 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    13021 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    13081 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    13141 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    13201 cacatttccc cgaaaagtgc cac
//
