LOCUS       sequence-246531-       10495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector that expresses wild type Flag-tagged human USP7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Maertens GN, El Messaoudi-Aubert S, Elderkin S, Hiom K, Peters G
  TITLE     Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation 
            of the INK4a tumour suppressor.
  JOURNAL   EMBO J. 2010 Aug 4;29(15):2553-65. doi: 10.1038/emboj.2010.129. Epub
            2010 Jul 2.
  PUBMED    20601937
REFERENCE   2  (bases 1 to 10495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010.129"; journalName: "EMBO J"; date: "2010-08-4- 
            4"; volume: "29"; issue: "15"; pages: "2553-65"
COMMENT     Sequence Label: pQFlag-USP7 WT puroR
FEATURES             Location/Qualifiers
     source          1..10495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2268..2291
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5622..6195
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5789..5806)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6016..6035
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6228..6827
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6228..6247)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6724..6744
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7201..7626
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7729..7751)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7887..7907)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7892..8221
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8072..8207
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8243..8262
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8343..8360)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8514..9102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8594..8613)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9273..10064,10065..10133))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9896..9915
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10134..10238)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10306..10324
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10424..10495,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgcg acaccatggc acaattggat tacaaggatg
     2281 acgatgacaa gaccggtatg aaccaccagc agcagcagca gcagcagaaa gcgggcgagc
     2341 agcagttgag cgagcccgag gacatggaga tggaagcggg agatacagat gacccaccaa
     2401 gaattactca gaaccctgtg atcaatggga atgtggccct gagtgatgga cacaacaccg
     2461 cggaggagga catggaggat gacaccagtt ggcgctccga ggcaaccttt cagttcactg
     2521 tggagcgctt cagcagactg agtgagtcgg tccttagccc tccgtgtttt gtgcgaaatc
     2581 tgccatggaa gattatggtg atgccacgct tttatccaga cagaccacac caaaaaagcg
     2641 taggattctt tctccagtgc aatgctgaat ctgattccac gtcatggtct tgccatgcac
     2701 aagcagtgct gaagataata aattacagag atgatgaaaa gtcgttcagt cgtcgtatta
     2761 gtcatttgtt cttccataaa gaaaatgatt ggggattttc caattttatg gcctggagtg
     2821 aagtgaccga tcctgagaaa ggatttatag atgatgacaa agttaccttt gaagtctttg
     2881 tacaggcgga tgctccccat ggagttgcgt gggattcaaa gaagcacaca ggctacgtcg
     2941 gcttaaagaa tcagggagcg acttgttaca tgaacagcct gctacagacg ttatttttca
     3001 cgaatcagct acgaaaggct gtgtacatga tgccaaccga gggggatgat tcgtctaaaa
     3061 gcgtcccttt agcattacaa agagtgttct atgaattaca gcatagtgat aaacctgtag
     3121 gaacaaaaaa gttaacaaag tcatttgggt gggaaacttt agatagcttc atgcaacatg
     3181 atgttcagga gctttgtcga gtgttgctcg ataatgtgga aaataagatg aaaggcacct
     3241 gtgtagaggg caccataccc aaattattcc gcggcaaaat ggtgtcctat atccagtgta
     3301 aagaagtaga ctatcggtct gatagaagag aagattatta tgatatccag ctaagtatca
     3361 aaggaaagaa aaatatattt gaatcatttg tggattatgt ggcagtagaa cagctcgatg
     3421 gggacaataa atacgacgct ggggaacatg gcttacagga agcagagaaa ggtgtgaaat
     3481 tcctaacatt gccaccagtg ttacatctac aactgatgag atttatgtat gaccctcaga
     3541 cggaccaaaa tatcaagatc aatgataggt ttgaattccc agagcagtta ccacttgatg
     3601 aatttttgca aaaaacagat cctaaggacc ctgcaaatta tattcttcat gcagtcctgg
     3661 ttcatagtgg agataatcat ggtggacatt atgtggttta tctaaacccc aaaggggatg
     3721 gcaaatggtg taaatttgat gacgacgtgg tgtcaaggtg tactaaagag gaagcaattg
     3781 agcacaatta tgggggtcac gatgacgacc tgtctgttcg acactgcact aatgcttaca
     3841 tgttagtcta catcagggaa tcaaaactga gtgaagtttt acaggcggtc accgaccatg
     3901 atattcctca gcagttggtg gagcgattac aagaagagaa aaggatcgag gctcagaagc
     3961 ggaaggagcg gcaggaagcc catctctata tgcaagtgca gatagtcgca gaggaccagt
     4021 tttgtggcca ccaagggaat gacatgtacg atgaagaaaa agtgaaatac actgtgttta
     4081 aagtattgaa gaactcctcg cttgctgagt ttgttcagag cctctctcag accatgggat
     4141 ttccacaaga tcaaattcga ttgtggccca tgcaagcaag gagtaatgga acaaaacgac
     4201 cagcaatgtt agataatgaa gccgacggca ataaaacaat gattgagctc agtgataatg
     4261 aaaacccttg gacaatattc ctggaaacag ttgatcccga gctggctgct agtggagcga
     4321 ccttacccaa gtttgataaa gatcatgatg taatgttatt tttgaagatg tatgatccca
     4381 aaacgcggag cttgaattac tgtgggcata tctacacacc aatatcctgt aaaatacgtg
     4441 acttgctccc agttatgtgt gacagagcag gatttattca agatactagc cttatcctct
     4501 atgaggaagt taaaccgaat ttaacagaga gaattcagga ctatgacgtg tctcttgata
     4561 aagcccttga tgaactaatg gatggtgaca tcatagtatt tcagaaggat gaccctgaaa
     4621 atgataacag tgaattaccc accgcaaagg agtatttccg agatctctac caccgcgttg
     4681 atgtcatttt ctgtgataaa acaatcccta atgatcctgg atttgtggtt acgttatcaa
     4741 atagaatgaa ttattttcag gttgcaaaga cagttgcaca gaggctcaac acagatccaa
     4801 tgttgctgca gtttttcaag tctcaaggtt atagggatgg cccaggtaat cctcttagac
     4861 ataattatga aggtacttta agagatcttc tacagttctt caagcctaga caacctaaga
     4921 aactttacta tcagcagctt aagatgaaaa tcacagactt tgagaacagg cgaagtttta
     4981 aatgtatatg gttaaacagc caatttaggg aagaggaaat aacactatat ccagacaagc
     5041 atgggtgtgt ccgggacctg ttagaagaat gtaaaaaggc cgtggagctt ggggagaaag
     5101 catcagggaa acttaggctg ctagaaattg taagctacaa aatcattggt gttcatcaag
     5161 aagatgaact attagaatgt ttatctcctg caacgagccg gacgtttcga atagaggaaa
     5221 tccctttgga ccaggtggac atagacaaag agaatgagat gcttgtcaca gtggcgcatt
     5281 tccacaaaga ggtcttcgga acgttcggaa tcccgttttt gctgaggata caccagggcg
     5341 agcattttcg agaagtgatg aagcgaatcc agagcctgct ggacatccag gagaaggagt
     5401 ttgagaagtt taaatttgca attgtaatga tgggccgaca ccagtacata aatgaagacg
     5461 agtatgaagt aaatttgaaa gactttgagc cacagcccgg taatatgtct catcctcggc
     5521 cttggctagg gctcgaccac ttcaacaaag ccccaaagag gagtcgctac acttaccttg
     5581 aaaaggccat taaaatccat aactgaggat ccggaattcc gcccccctcc ctcccccccc
     5641 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     5701 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     5761 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     5821 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     5881 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     5941 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     6001 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     6061 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     6121 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     6181 aaaacacgat gataagcttg ccacaaccca caaggagacg accttccatg accgagtaca
     6241 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
     6301 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
     6361 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     6421 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6481 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     6541 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     6601 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     6661 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     6721 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     6781 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     6841 acccgcagcg cccgaccgaa aggagcgcac gaccccatgg ctccgaccga agccacccgg
     6901 ggcggccccg ccgaccccgc acccgccccc gaggcccacc gactctagtc gagggctgca
     6961 gcgctgcaga ggccgagtgc agaactgctc caaagggacc tcaaggcttt ccgagggaca
     7021 ctaggctgac tccatcgagc cagtgtagag ataagcttat cgattagtcc aatttgttaa
     7081 agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc
     7141 tatagagtac gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg
     7201 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     7261 atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac
     7321 agggtcgacc ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     7381 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     7441 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
     7501 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     7561 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     7621 ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac
     7681 cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     7741 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     7801 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     7861 cgatagcgga gtgtagatcc ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7921 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7981 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8041 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8101 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8161 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     8221 aaagcttact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     8281 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     8341 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8401 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8461 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8521 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8581 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8641 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8701 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8761 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8821 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8881 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8941 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9001 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9061 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9121 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9181 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9241 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9301 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9361 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9421 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9481 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9541 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     9601 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9661 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9721 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9781 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9841 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     9901 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9961 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10021 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10081 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10141 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10201 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10261 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    10321 aggccctttc gtcttcaaga attagcttgg ccattgcata cgttgtatcc atatcataat
    10381 atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg attattgact
    10441 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagt
//
