LOCUS       sequence-246534-        6492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6492)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-TBRII-C-18
FEATURES             Location/Qualifiers
     source          1..6492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      611..620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             617..1324
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(762..780)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1030..1049
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2706..2717
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    3284..3405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3321..3340)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3375..3394
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3412..3867)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3549..3570)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3761..3780
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3894..3998
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4000..4357
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4000..4191
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4023..4043)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4208..4343
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4392..5186
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4392..5186
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4446..4465)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5056..5075
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5374..5393)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5418..5465
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5794..6382
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6283..6302
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
      601 gctaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
      661 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      721 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      781 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
      841 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
      901 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
      961 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     1021 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     1081 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     1141 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     1201 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     1261 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     1321 caagtccgga ctcagatctc gagagagtgg ctctggtggg tcgagtggaa gtggcagcgg
     1381 tcgggggctg ctcaggggcc tgtggccgct gcacatcgtc ctgtggacgc gtatcgccag
     1441 cacgatccca ccgcacgttc agaagtcgga tgtggaaatg gaggcccaga aagatgaaat
     1501 catctgcccc agctgtaata ggactgccca tccactgaga catattaata acgacatgat
     1561 agtcactgac aacaacggtg cagtcaagtt tccacaactg tgtaaatttt gtgatgtgag
     1621 attttccacc tgtgacaacc agaaatcctg catgagcaac tgcagcatca cctccatctg
     1681 tgagaagcca caggaagtct gtgtggctgt atggagaaag aatgacgaga acataacact
     1741 agagacagtt tgccatgacc ccaagctccc ctaccatgac tttattctgg aagatgctgc
     1801 ttctccaaag tgcattatga aggaaaaaaa aaagcctggt gagactttct tcatgtgttc
     1861 ctgtagctct gatgagtgca atgacaacat catcttctca gaagaatata acaccagcaa
     1921 tcctgacttg ttgctagtca tatttcaagt gacaggcatc agcctcctgc caccactggg
     1981 agttgccata tctgtcatca tcatcttcta ctgctaccgc gttaaccggc agcagaagct
     2041 gagttcaacc tgggaaaccg gcaagacgcg gaagctcatg gagttcagcg agcactgtgc
     2101 catcatcctg gaagatgacc gctctgacat cagctccacg tgtgccaaca acatcaacca
     2161 caacacagag ctgctgccca ttgagctgga caccctggtg gggaaaggtc gctttgctga
     2221 ggtctataag gccaagctga agcagaacac ttcagagcag tttgagacag tggcagtcaa
     2281 gatctttccc tatgaggagt atgcctcttg gaagacagag aaggacatct tctcagacat
     2341 caatctgaag catgagaaca tactccagtt cctgacggct gaggagcgga agacggagtt
     2401 ggggaaacaa tactggctga tcaccgcctt ccacgccaag ggcaacctac aggagtacct
     2461 gacgcggcat gtcatcagct gggaggacct gcgcaagctg ggcagctccc tcgcccgggg
     2521 gattgctaac ctccacagtg atcacactcc atgtgggagg cccaagatgc ccatcgtgca
     2581 cagggacctc aagagctcca atatcctcgt gaagaacgac ctaacctgct gcctgtgtga
     2641 ctttgggctt tccctgcgtc tggaccctac tctgtctgtg gatgacctgg ctaacagtgg
     2701 gcaggtggga actgcaagat acatggctcc agaagtccta gaatccagga tgaatttgga
     2761 gaatgttgag tccttcaagc agaccgatgt ctactccatg gctctggtgc tctgggaaat
     2821 gacatctcgc tgtaatgcag tgggagaagt aaaagattat gagcctccat ttggttccaa
     2881 ggtgcgggag cacccctgtg tcgaaagcat gaaggacaac gtgttgagag atcgagggcg
     2941 accagaaatt cccagcttct ggctcaacca ccagggcatc cagatggtgt gtgagacgtt
     3001 gactgagtgc tgggaccacg acccagaggc ccgtctcaca gcccagtgtg tggcagaacg
     3061 cttcagtgag ctggagcatc tggacaggct ctcggggagg agctgctcgg aggagaagat
     3121 tcctgaagac ggctccctaa acactaccaa atagggatcc accggatcta gataactgat
     3181 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     3241 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     3301 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3361 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa
     3421 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     3481 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     3541 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     3601 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     3661 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     3721 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     3781 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     3841 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3901 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3961 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     4021 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4081 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4141 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4201 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4261 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4321 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     4381 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     4441 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     4501 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     4561 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     4621 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     4681 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     4741 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     4801 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     4861 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     4921 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     4981 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     5041 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     5101 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     5161 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     5221 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     5281 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     5341 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     5401 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     5461 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     5521 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     5581 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     5641 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5701 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5761 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5821 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5881 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5941 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6001 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6061 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6121 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6181 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6241 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6301 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6361 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6421 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6481 tattaccgcc at
//
