LOCUS       sequence-246535-       13355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene 
            (NP_001074527.1 isoform) cloned into a dox-inducible piggybac 
            destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13355)
  AUTHORS   Insco ML, Abraham BJ, Dubbury SJ, Kaltheuner IH, Dust S, Wu C, Chen 
            KY, Liu D, Bellaousov S, Cox AM, Martin BJE, Zhang T, Ludwig CG, 
            Fabo T, Modhurima R, Esgdaille DE, Henriques T, Brown KM, Chanock 
            SJ, Geyer M, Adelman K, Sharp PA, Young RA, Boutz PL, Zon LI
  TITLE     Oncogenic CDK13 mutations impede nuclear RNA surveillance.
  JOURNAL   Science. 2023 Apr 21;380(6642):eabn7625. doi: 
            10.1126/science.abn7625. Epub 2023 Apr 21.
  PUBMED    37079685
REFERENCE   2  (bases 1 to 13355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-04-21"; volume: "380(6642):eabn7625. doi"; pages: " 
            10.1126/science.abn7625. Epub 2023 Apr 21"
COMMENT     Sequence Label: PB-Neo-TetO-Cdk13_Nterm_FlagHa
FEATURES             Location/Qualifiers
     source          1..13355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   746..808
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1021..1245
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(1305..1346)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    1348..1372
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2395..2412)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             complement(5925..5951)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(5952..5975)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      5975..5984
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6014..6038)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6150..6174)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6171..6209)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(6242..6260,6261..6283,6284..6302,6303..6325,6326..6344,
                     6345..6367,6368..6386,6387..6409,6410..6428,6429..6451,
                     6452..6470,6471..6493,6494..6512)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     polyA_signal    6615..6726
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(6764..7567)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6875..6894)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7485..7504
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(7629..7648)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8022..8045
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        complement(8071..8280)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8076..8211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8081..8378)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(8130..8149)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8419..8435
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8420..8436
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8785..8819
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9312..9328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9312..9328)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9325..9347)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9336..9352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9360..9366,9367..9384,9385..9390))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9405..9426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9543..9560)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9667..10170
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     10211..10229
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10267..10289)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10389..10408
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10432..10647
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10648..11451
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(11608..12196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11688..11707)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12367..13158,13159..13227))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12990..13009
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13228..13332)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gtgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa acaaaaactc aaaatttctt
      961 ctataaagta acaaaacttt tatcgaattc ctgcagccca gcttgatccc gcctcagaag
     1021 ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc
     1081 tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa
     1141 tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca
     1201 cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag gctgatcagc
     1261 gggtttaaac gggccctcta gaggttagcg ctagctcatt actacgtaga atcgagaccg
     1321 aggagagggt tagggatagg cttaccaacc actttgtaca agaaagctgg gtcgaattcg
     1381 cccttctcga gtcagtatgg taaccctctg cctcgacctc tgccagctga tgtgctaatg
     1441 tgtcccctgt atccttgtga atatccaggt ccttgggcag gagaagtcca ggtctgtcca
     1501 tgcatgaggc tagggccacc tggattttcc tgtaagttac caccatagtt atagttgtgc
     1561 atctttgctg gcaagggatg agtactctct ggccctgtgg aagggtcatt gctgtttgtg
     1621 aggactgtga taggaccatg gctatattca aatctatggt ccttgtctcc actaaacaac
     1681 atgggaccag cattgaggta aatgtctcca tatccagcta ctgaactggc aaacgactca
     1741 gtaaaggcag aagtttgagg tggaccacca gtgtgagatg aagcagtact gtagttatcc
     1801 agagactgaa tatctgagtt tccaatgaaa ctacgtgctt ccaatggtgc agcagcggag
     1861 ctacttccta gaccatcact gctaacgtaa ggagcggcag agaaagaaga tccaaagttg
     1921 tccttatagt ctgaactggg cacatatgtg tctcctgtgg gataatcgat accaccttct
     1981 cttttgggat catcctgggg ctgatgctga gcaagcagct gtaagagggc tgccttcact
     2041 ccagcatgtg ggtcagttaa tgaagaacta gtggtaggaa catgctggtt ttctgtccga
     2101 taatctaggt cctcctcagt aactggtggt ggttcaggag gttcaggagg ccgttgatca
     2161 ggaggcagaa ttcgaggccg gtctggttct ggtgtcagct ccaatatcct cctgtcttgg
     2221 tgctggatga ggtcatcttg cccggatcca ggagtgctcg gttctagagg aggcctgagt
     2281 tgcaatggag cttcatgtcc tgatccattt tccctttcct ctagcatggc atctttctgt
     2341 ttggactgtt gggcctttat taactgagtc aagaggactg caaatgcact ctgcacagca
     2401 gcctgggcag catcagtttc cactttaggc tggatggtct gtgaaggctg aactcctccc
     2461 aatgattttg aagactcctg ttgtggtgtt gatggatctg tctgtttttc acctgttgcc
     2521 aaaattccag caggaaggtt tattttattt agctgctgtt gagtctcaga gtttacctta
     2581 atgttcaaca cttggacaaa atcagccata ttaacacttg atttagattg tagtaggttt
     2641 agaagaattg ccaattcact gtggtttaac ggctgtccag ggcctgttat tacaggtgct
     2701 acatttgagt tgctctgaga tttcaactgt gcgggtggca gcacaccctg gggtgtgtta
     2761 gttctgctgt catctaagcc cagagacagg tccttcctag gggctttgat tgtggaaaga
     2821 tcatcagtca tgcccatctg tttctgtctt cttctctttt tactccataa ttcatgacaa
     2881 tcttgccaca aaggaaggtc tggtggaggc attttggagg gttccacgtc tcgcaggaac
     2941 tcacactgaa gagcctgctc agcagtgcag cgcttactgg gatccaaggc aagcatgtaa
     3001 tcgaataagt cgagtgcagc tgcggggatg aaaacaaatt cttctcttaa cttccgccga
     3061 tattgcttct ttggtttcat ggtgttgaaa tatggcagtt tgattacatc aggccacact
     3121 gcaggacatg gactcccaca tatacggctt attagctcta gctgtgcaag ttcctggttt
     3181 gcttgaaata taggtttttt agtgaagagt tcaccaagga tacatccaca gctccataca
     3241 tcaatggctg gtgtatatcg ttcttctccc aagagcaatt caggtggacg ataccacaaa
     3301 gtaatgacct tgttagtata tgggcgactt tcttctgagc tatataaacg agcaagtcca
     3361 aaatctgcaa gttttatctg tcctctatta tttagaagga tatttgaaca tttaatatct
     3421 ctatgcaaaa agttcttctt atgacaataa tccaggcctt ccatgagctg tctcataaaa
     3481 gattttatat ggttttcatt aaaatgaacc aagcctgatt ccagcagtcc catcagatca
     3541 tggtccatat attcaaacac caggtaaaat gcacctttgt ctttcttaaa atccaaagca
     3601 tcttctttat cagtcacgat ttccttcata ttgatgatac tctggtgggt gagttgccga
     3661 agaattttaa tttctctaat tgctgtaatt gggaaaccct ccttttcatt atccagacgt
     3721 actttcttta aggctaccat ttctcccgtg tctttgtccc tggctttgta aacttgtcca
     3781 taagtacctt ctccaataat tccgatgata tcaaatttat ccacgcagcg tttcccccag
     3841 tcaatatctt tttctttgat ttcaccatag cgaggcccac atattttagg ccttcgtttg
     3901 ctatgtaact gtgctgctgt tttcttctcc tctggactct tggaaagatc atctcctcct
     3961 ggtaactcag ggggcaatgg taaatcagca agcagacatc ggagtttctt ttctacttct
     4021 tttttaactg ctttgacaga aatgttgcct cttaagctat cagcatcttt atcttcaggc
     4081 agcatgggag gcaaaggtaa tggcggtaac gtagaggtca ctaaagccac atgctgctcc
     4141 ttctccttgg ctcctgtgct tggtgtgagt ggcttgcttt tctctttagt ggaaactggt
     4201 tcttcctttg tagcagcagg tttactctcc ttcccaacaa ctgttttctt ggtggctttc
     4261 tctactgtca aattattgtc tacctttgtt acttgaagcg gtggctttgt ttttgctttg
     4321 tcgcttttga cggtcccacc acttgaagga gaaggtgcat gctcagtttt aagcttcttg
     4381 acttccttca cgtggttggt ctgggagacg gaggctccgg tctcggtgtt tcccttggtg
     4441 ggtgtggaag cgttggaggc cttagcagcc ttggcggcgg cttcagcagc tttggcggct
     4501 tcggcggctt tagcggcttc ggcagctcta gcagcctcag cggccctagc tttcttgttc
     4561 ttgttgagct cggcggccag ggagctcttc agtgtcaggg tggaggggct gatggaggag
     4621 tgcctgcttc tggacctgct cagcctgtgc ctggacctgc ttctgctgtg cctggaggag
     4681 taagggctcc tggacctgga cttggcggac ctcctcagca caggggaata gggggatctg
     4741 ctcctcctcc aggaggagga gctgtagggg gagggggaga catctcctcc tctctcgtag
     4801 gaggaatgcc tgctgtagct gggggatctc ctcctgctgt aggggctagg ggacctgggc
     4861 agtcttctgg aataagggct gcttccgcct ccagcggggg aagggctttt cctggatctc
     4921 agggactggc tagccctgtg gctgacaggg gactcgtctc tgcctccgag agggctgagg
     4981 gacctctgcc tcctcctgta ggccttgggt tcggacttgt cttccctgta ggctttgggg
     5041 ggctccttgt aggcggaagg gggttccttg gagctcttgg tcctgctcct atgagccttg
     5101 aggtccctgt ccttcctgga gctgctgctg gaggatgtgg cggaggcgga ctttcttctt
     5161 ccgccggagg agctggagga gccggatttg gcagcctcgg ctctttcctc gccgctgtgt
     5221 ccgtgtctgg atctggcctt gctggcctcg gatccgctcc ttgtgccatc tctcctccta
     5281 tgttccctgt gcctctcttt ggacctgccg ctggagctcc ttctgtccct tctaggtctt
     5341 ctctcgctgc cctcggctct tctctgggtt ccgctgctgg aagcggggga tcctccattt
     5401 cctccggttc ctccagcagc ggtggcagct gtagcagcgc tagctcctcc caggagcagg
     5461 ccttgttcgc tttggctgga cacgtcctcg tactccacca ggggtgtcac tcctcctccg
     5521 ctggaagctc ctccgcctcc atcctgctgg ggttgaggca gggagaacac tctcctcttt
     5581 tcggcttcct gtccggccct aggtcctctc cttctctttt gcctgccgcc gggtctcctt
     5641 tttcctctgg ccagcctctt gacctccaga ggagggccag ggctaaaaca ggagctggaa
     5701 gcggcagcag cagcggcggc agcggcagcg gctccaggag cagccagaaa caggagagga
     5761 gggggaggag gagggggttg gaggagctga ggttgcagga gagggagcag cagaggaggc
     5821 tgctgagggc tgagaaacct cctcctcttc ctcctctcct ccagcttttt ctcggcccag
     5881 ctgagtccgc ctcctccgcc gagagctgta tcggagctgg aaggagcgta atcgggcacg
     5941 tcataagggt acttgtcatc gtcgtccttg tagtccatgg tggcggcggt accagtagta
     6001 agggcgaatt cggagcctgc ttttttgtac aaacttgttg atatctgcac tagtggatcc
     6061 gagctcggta cgaattcggg gccgcggagg ctggatcggt cccggtgtct tctatggagg
     6121 tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg agctctgctt
     6181 atataggcct cccaccgtac acgcctacct cgacccgggt accgagctcg actttcactt
     6241 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
     6301 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
     6361 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
     6421 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
     6481 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgagttgc ctgctattgt
     6541 cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc
     6601 tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac
     6661 cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag
     6721 gcacagtcga ggctgatcag cgagctctag agaattgatc ccctcagaag aactcgtcaa
     6781 gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga
     6841 agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt
     6901 cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat
     6961 tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga tcatcgccgt
     7021 cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt
     7081 cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc
     7141 gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca
     7201 ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct
     7261 gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca
     7321 cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca
     7381 gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg
     7441 acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga
     7501 atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatggccg
     7561 atcccatatt ggctgcaggt cgaaaggccc ggagatgagg aagaggagaa cagcgcggca
     7621 gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc
     7681 ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag cctctgagcc
     7741 cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat
     7801 tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc catttgtcac
     7861 gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg aggagtagaa
     7921 ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt ggatgtggaa
     7981 tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg
     8041 catgctccag actgccttgg gaaaagtact gcctaggcct ccaaaaaagc ctcctcacta
     8101 cttctggaat agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag
     8161 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     8221 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     8281 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     8341 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cacagcagag
     8401 atccagtttg gttagatctc gaggtcgacg gtatcgttgg ccggtactcg acgtagggat
     8461 aagatcggaa gagcggttca gcaggaatgc cgagaccgcg ccaacgataa gctatctata
     8521 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata acaatataat
     8581 tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca
     8641 cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg
     8701 agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa
     8761 agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt
     8821 taatctagct gcatcaggat catatcgtcg ggtctttttt caattgctca gtcatcgccc
     8881 aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga
     8941 agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg
     9001 cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt
     9061 cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt
     9121 cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg
     9181 tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc
     9241 tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcttg
     9301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9481 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gacctgggtc
     9661 cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa
     9721 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg
     9781 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc
     9841 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg
     9901 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt
     9961 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt
    10021 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca
    10081 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac
    10141 gaggcgcgtg taagttacag gcaagcgatc cgtctaagaa accattatta tcatgacatt
    10201 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    10261 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    10321 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    10381 taactatgcg gcatcagagc agattgtact gagagtgcac cataccacct tttcaattca
    10441 tcattttttt tttattcttt tttttgattt cggtttcctt gaaatttttt tgattcggta
    10501 atctccgaac agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc
    10561 atatgtagtg ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca
    10621 aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct
    10681 actcatccta gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac
    10741 ttgtgtgctt cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta
    10801 ggtcccaaaa tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag
    10861 ggcacagtta agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaagac
    10921 agaaaatttg ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga
    10981 atagcagaat gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc
    11041 ggtttgaagc aggcggcaga agaagtaaca aaggaaccta gaggcctttt gatgttagca
    11101 gaattgtcat gcaagggctc cctatctact ggagaatata ctaagggtac tgttgacatt
    11161 gcgaagagcg acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga
    11221 gatgaaggtt acgattggtt gattatgaca cccggtgtgg gtttagatga caagggagac
    11281 gcattgggtc aacagtatag aaccgtggat gatgtggtct ctacaggatc tgacattatt
    11341 attgttggaa gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac
    11401 agaaaagcag gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta
    11461 ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta attatatcag
    11521 ttattaccct atgcggtgtg aaataccgca cagagcaaaa ggccagcaaa aggccaggaa
    11581 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    11641 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    11701 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    11761 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    11821 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    11881 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    11941 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    12001 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    12061 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    12121 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    12181 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    12241 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    12301 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    12361 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    12421 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    12481 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    12541 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    12601 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    12661 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    12721 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    12781 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    12841 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    12901 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    12961 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    13021 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    13081 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    13141 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    13201 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    13261 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    13321 aggggttccg cgcacatttc cccgaaaagt gccac
//
