LOCUS       sequence-246538-       15211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15211)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX047
FEATURES             Location/Qualifiers
     source          1..15211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5325..5349)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5378..5467
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5501..5753
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6182..6434)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6902..7927)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6902..7927)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7937..8281)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8423..8440)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8423..8439)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8432..8454)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8579..8598)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8789..8810
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9057..9081
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10677..11825)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12124..12918)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13110..13877
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14570..15201
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggtttc
     4621 accggaaact accaactggt tatacgatta caatttcgaa gatataactg tgtctgttga
     4681 tcccaatttc tcaaattcaa catctgggtt ttcttggtct atgcaaccct ttaatggttc
     4741 aacaactaac atcagtgttg atattgatgg ctcaattggt gaatccgact gcctgaagga
     4801 aagcggttct aagaaaaggg caagagctga atcatgcacc tcgtcaagtt ccaaagcttg
     4861 cagggagaaa ttaaggagag agaagctgaa tgacaagttc atggaattgg gtgcacttct
     4921 tgagcctgga agaactccaa aaacagacaa gagcgccatt ctggttgatg ctgttcgcat
     4981 ggtgacccat ttacgcggtg aggctgaaaa gctgaaaggc tcaaatttga atctacaaga
     5041 gaagataaag gagttgaagg ccgagaaaaa tgagcttcga gatgaaaagc aaaggcttaa
     5101 ggctgataaa gagaagctag agcaacaact aaagactatg aatgcacaac ctggtttctt
     5161 tcctcctgtc gtaccaggtg cttttgctgc gccaggtcaa gctgcaggaa gcaagctggt
     5221 gccgattata agttaccccg gggtcgccat gtggcagtac atgcctcctg ctgcagtaga
     5281 cacgtcgcaa gatcatgttc tccgacctcc agttgctccc ggggacccag ctttcttgta
     5341 caaagtggtt gataacagcg ggttaattaa catcttttac ccatacgatg ttcctgacta
     5401 tgcgggctat ccctatgacg tcccggacta tgcaggatcc tatccatatg acgttccaga
     5461 ttacgctgct cagtgcagcg cttagagctc gaatttcccc gatcgttcaa acatttggca
     5521 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     5581 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     5641 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     5701 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     5761 agcttcatca acgcaagaca tgcgcacgac cgtctgacag gagaggaatt tccgacgagc
     5821 acagaaagga cttgctcttg gacgtaggcc tatttctcag gcacatgtat caagtgttcg
     5881 gacgtgggtt ttcgatggtg tatcagccgc cgccaactgg gagatgagga ggctttcttg
     5941 gggggcagtc agcagttcat ttcacaagac agaggaactt gtaaggagat gcactgattt
     6001 atcttggcgc aaaccagcag gacgaattag tgggaatagc ccgcgaatat ctaagttatg
     6061 cctgtcggca tgagcagaaa cttccaattc gaaacagttt ggagaggttg tttttgggca
     6121 taccttttgt tagtcagcct ctcgattgct catcgtcatt acacagtacc gaagtttgat
     6181 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
     6241 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
     6301 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
     6361 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
     6421 atgtttgaac gatctgcttc gacgcactcc ttctttactc caccatctcg tccttattga
     6481 aaacgtgggt agcaccaaaa cgaatcaagt cgctggaact gaagttacca atcacgctgg
     6541 atgatttgcc agttggatta atcttgcctt tccccgcatg aataatattg atgaatgcat
     6601 gcgtgagggg tatttcgatt ttggcaatag ctgcaattgc cgcgacatcc tccaacgagc
     6661 ataattcttc agaaaaatag cgatgttcca tgttgtcagg gcatgcatga tgcacgttat
     6721 gaggtgacgg tgctaggcag tattccctca aagtttcata gtcagtatca tattcatcat
     6781 tgcattcctg caagagagaa ttgagacgca atccacacgc tgcggcaacc ttccggcgtt
     6841 cgtggtctat ttgctcttgg acgttgcaaa cgtaagtgtt ggatcccggt cggcatctac
     6901 tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt
     6961 ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag
     7021 tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga
     7081 aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc
     7141 ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct
     7201 ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc
     7261 cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat
     7321 tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa
     7381 gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt
     7441 gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt
     7501 gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag
     7561 cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca
     7621 ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga
     7681 attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa
     7741 cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc
     7801 ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg
     7861 agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct
     7921 ttttcatatc ggggtcgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc
     7981 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
     8041 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
     8101 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
     8161 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
     8221 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
     8281 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
     8341 gtgctccacc atgttgacgg atctctagga cgcgtcgacg cgtcgacgcg tcgacgcgtc
     8401 gacgcgtcct agaagctaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     8461 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     8521 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgcccgct
     8581 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8641 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8701 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8761 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     8821 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggaaccacca
     8881 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     8941 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
     9001 ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     9061 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     9121 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     9181 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     9241 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     9301 gcgttgctgc ctgtgatcaa atatcatctc cctcgcagag atccgaatta tcagccttct
     9361 tattcatttc tcgcttaacc gtgacaggct gtcgatcttg agaactatgc cgacataata
     9421 ggaaatcgct ggataaagcc gctgaggaag ctgagtggcg ctatttcttt agaagtgaac
     9481 gttgacgata tcaactcccc tatccattgc tcaccgaatg gtacaggtcg gggacccgaa
     9541 gttccgactg tcggcctgat gcatccccgg ctgatcgacc ccagatctgg ggctgagaaa
     9601 gcccagtaag gaaacaactg taggttcgag tcgcgagatc ccccggaacc aaaggaagta
     9661 ggttaaaccc gctccgatca ggccgagcca cgccaggccg agaacattgg ttcctgtagg
     9721 catcgggatt ggcggatcaa acactaaagc tactggaacg agcagaagtc ctccggccgc
     9781 cagttgccag gcggtaaagg tgagcagagg cacgggaggt tgccacttgc gggtcagcac
     9841 ggttccgaac gccatggaaa ccgcccccgc caggcccgct gcgacgccga caggatctag
     9901 cgctgcgttt ggtgtcaaca ccaacagcgc cacgcccgca gttccgcaaa tagcccccag
     9961 gaccgccatc aatcgtatcg ggctacctag cagagcggca gagatgaaca cgaccatcag
    10021 cggctgcaca gcgcctaccg tcgccgcgac cccgcccggc aggcggtaga ccgaaataaa
    10081 caacaagctc cagaatagcg aaatattaag tgcgccgagg atgaagatgc gcatccacca
    10141 gattcccgtt ggaatctgtc ggacgatcat cacgagcaat aaacccgccg gcaacgcccg
    10201 cagcagcata ccggcgaccc ctcggcctcg ctgttcgggc tccacgaaaa cgccggacag
    10261 atgcgccttg tgagcgtcct tggggccgtc ctcctgtttg aagaccgaca gcccaatgat
    10321 ctcgccgtcg atgtaggcgc cgaatgccac ggcatctcgc aaccgttcag cgaacgcctc
    10381 catgggcttt ttctcctcgt gctcgtaaac ggacccgaac atctctggag ctttcttcag
    10441 ggccgacaat cggatctcgc ggaaatcctg cacgtcggcc gctccaagcc gtcgaatctg
    10501 agccttaatc acaattgtca attttaatcc tctgtttatc ggcagttcgt agagcgcgcc
    10561 gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag cagtgcccgc ttgttcctga
    10621 aatgccagta aagcgctggc tgctgaaccc ccagccggaa ctgaccccac aaggccctag
    10681 cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc
    10741 aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat
    10801 ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa
    10861 tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc
    10921 gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg
    10981 gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg
    11041 tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc
    11101 tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag
    11161 aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc
    11221 cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc
    11281 ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc
    11341 gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac
    11401 ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc
    11461 aacgcggcct gcttggcctc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc
    11521 ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc
    11581 tcctgttcga gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga
    11641 gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat cgagccgacg
    11701 gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc
    11761 tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga
    11821 ttcattcacc ctccttgcgg gattgccccg actcacgccg gggcaatgtg cccttattcc
    11881 tgatttgacc cgcctggtgc cttggtgtcc agataatcca ccttatcggc aatgaagtcg
    11941 gtcccgtaga ccgtctggcc gtccttctcg tacttggtat tccgaatctt gccctgcacg
    12001 aataccagcg accccttgcc caaatacttg ccgtgggcct cggcctgaga gccaaaacac
    12061 ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag
    12121 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
    12181 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
    12241 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
    12301 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
    12361 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
    12421 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
    12481 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
    12541 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
    12601 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
    12661 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
    12721 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
    12781 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
    12841 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
    12901 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
    12961 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
    13021 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
    13081 acggagccga ttttgaaacc acaattatgg gtgatgctgc caacttactg atttagtgta
    13141 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
    13201 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctatctctgc
    13261 tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca acccggtacg
    13321 caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac agcccgcatt
    13381 atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg cagtcggtaa
    13441 cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg
    13501 gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac agtctccgga
    13561 agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga
    13621 gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat
    13681 cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc
    13741 ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa
    13801 aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcaat
    13861 aagttggagt cattacccaa ttatgataga atttacaagc tataaggtta ttgtcctggg
    13921 tttcaagcat tagtccatgc aagtttttat gctttgccca ttctatagat atattgataa
    13981 gcgcgctgcc tatgccttgc cccctgaaat ccttacatac ggcgatatct tctatataaa
    14041 agatatatta tcttatcagt attgtcaata tattcaaggc aatctgcctc ctcatcctct
    14101 tcatcctctt cgtcttggta gctttttaaa tatggcgctt catagagtaa ttctgtaaag
    14161 gtccaattct cgttttcata cctcggtata atcttaccta tcacctcaaa tggttcgctg
    14221 ggtttatcgc acccccgaac acgagcacgg cacccgcgac cactatgcca agaatgccca
    14281 aggtaaaaat tgccggcccc gccatgaagt ccgtgaatgc cccgacggcc gaagtgaagg
    14341 gcaggccgcc acccaggccg ccgccctcac tgcccggcac ctggtcgctg aatgtcgatg
    14401 ccagcacctg cggcacgtca atgcttccgg gcgtcgcgct cgggctgatc gcccatcccg
    14461 ttactgcccc gatcccggca atggcaagga ctgccagcgc tgccattttt ggggtgaggc
    14521 cgttcgcggc cgaggggcgc agcccctggg gggatgggag gcccgcgtta gcgggccggg
    14581 agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag
    14641 ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag
    14701 cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc tgtggacagc
    14761 ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag
    14821 gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt
    14881 atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgccg
    14941 atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc
    15001 aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg
    15061 agggccaagt tttccgcgag gtatccacaa cgccggcggc cgcggtgtct cgcacacggc
    15121 ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg
    15181 gcaaccagcc cggtgagcgt cggaaaggcg c
//
