LOCUS       sequence-246546-        7380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into 
            Gateway entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Insco ML, Abraham BJ, Dubbury SJ, Kaltheuner IH, Dust S, Wu C, Chen 
            KY, Liu D, Bellaousov S, Cox AM, Martin BJE, Zhang T, Ludwig CG, 
            Fabo T, Modhurima R, Esgdaille DE, Henriques T, Brown KM, Chanock 
            SJ, Geyer M, Adelman K, Sharp PA, Young RA, Boutz PL, Zon LI
  TITLE     Oncogenic CDK13 mutations impede nuclear RNA surveillance.
  JOURNAL   Science. 2023 Apr 21;380(6642):eabn7625. doi: 
            10.1126/science.abn7625. Epub 2023 Apr 21.
  PUBMED    37079685
REFERENCE   2  (bases 1 to 7380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-04-21"; volume: "380(6642):eabn7625. doi"; pages: " 
            10.1126/science.abn7625. Epub 2023 Apr 21"
COMMENT     Sequence Label: pCR8/GW_TOPO_Cdk13_Untagged
FEATURES             Location/Qualifiers
     source          1..7380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4219..4236
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     protein_bind    complement(5259..5358)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5375..5394)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5376..5394)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5399..5415)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5399..5415)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5846..6637
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6730..7318
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7219..7238
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcgccc ttactactgg taccgccgcc accatgcctt ccagctccga
      721 tacagctctc ggcggaggag gcggactcag ctgggccgag aaaaagctgg aggagaggag
      781 gaagaggagg aggtttctca gccctcagca gcctcctctg ctgctccctc tcctgcaacc
      841 tcagctcctc caaccccctc ctcctccccc tcctctcctg tttctggctg ctcctggagc
      901 cgctgccgct gccgccgctg ctgctgccgc ttccagctcc tgttttagcc ctggccctcc
      961 tctggaggtc aagaggctgg ccagaggaaa aaggagaccc ggcggcaggc aaaagagaag
     1021 gagaggacct agggccggac aggaagccga aaagaggaga gtgttctccc tgcctcaacc
     1081 ccagcaggat ggaggcggag gagcttccag cggaggagga gtgacacccc tggtggagta
     1141 cgaggacgtg tccagccaaa gcgaacaagg cctgctcctg ggaggagcta gcgctgctac
     1201 agctgccacc gctgctggag gaaccggagg aaatggagga tcccccgctt ccagcagcgg
     1261 aacccagaga agagccgagg gcagcgagag aagacctaga agggacagaa ggagctccag
     1321 cggcaggtcc aaagagaggc acagggaaca taggaggaga gatggcacaa ggagcggatc
     1381 cgaggccagc aaggccagat ccagacacgg acacagcggc gaggaaagag ccgaggctgc
     1441 caaatccggc tcctccagct cctccggcgg aagaagaaag tccgcctccg ccacatcctc
     1501 cagcagcagc tccaggaagg acagggacct caaggctcat aggagcagga ccaagagctc
     1561 caaggaaccc ccttccgcct acaaggagcc ccccaaagcc tacagggaag acaagtccga
     1621 acccaaggcc tacaggagga ggcagaggtc cctcagccct ctcggaggca gagacgagtc
     1681 ccctgtcagc cacagggcta gccagtccct gagatccagg aaaagccctt cccccgctgg
     1741 aggcggaagc agcccttatt ccagaagact gcccaggtcc cctagcccct acagcaggag
     1801 gagatccccc agctacagca ggcattcctc ctacgagaga ggaggagatg tctccccctc
     1861 cccctacagc tcctcctcct ggaggaggag cagatccccc tattcccctg tgctgaggag
     1921 gtccgccaag tccaggtcca ggagccctta ctcctccagg cacagcagaa gcaggtccag
     1981 gcacaggctg agcaggtcca gaagcaggca ctcctccatc agcccctcca ccctgacact
     2041 gaagagctcc ctggccgccg agctcaacaa gaacaagaaa gctagggccg ctgaggctgc
     2101 tagagctgcc gaagccgcta aagccgccga agccgccaaa gctgctgaag ccgccgccaa
     2161 ggctgctaag gcctccaacg cttccacacc caccaaggga aacaccgaga ccggagcctc
     2221 cgtctcccag accaaccacg tgaaggaagt caagaagctt aaaactgagc atgcaccttc
     2281 tccttcaagt ggtgggaccg tcaaaagcga caaagcaaaa acaaagccac cgcttcaagt
     2341 aacaaaggta gacaataatt tgacagtaga gaaagccacc aagaaaacag ttgttgggaa
     2401 ggagagtaaa cctgctgcta caaaggaaga accagtttcc actaaagaga aaagcaagcc
     2461 actcacacca agcacaggag ccaaggagaa ggagcagcat gtggctttag tgacctctac
     2521 gttaccgcca ttacctttgc ctcccatgct gcctgaagat aaagatgctg atagcttaag
     2581 aggcaacatt tctgtcaaag cagttaaaaa agaagtagaa aagaaactcc gatgtctgct
     2641 tgctgattta ccattgcccc ctgagttacc aggaggagat gatctttcca agagtccaga
     2701 ggagaagaaa acagcagcac agttacatag caaacgaagg cctaaaatat gtgggcctcg
     2761 ctatggtgaa atcaaagaaa aagatattga ctgggggaaa cgctgcgtgg ataaatttga
     2821 tatcatcgga attattggag aaggtactta tggacaagtt tacaaagcca gggacaaaga
     2881 cacgggagaa atggtagcct taaagaaagt acgtctggat aatgaaaagg agggtttccc
     2941 aattacagca attagagaaa ttaaaattct tcggcaactc acccaccaga gtatcatcaa
     3001 tatgaaggaa atcgtgactg ataaagaaga tgctttggat tttaagaaag acaaaggtgc
     3061 attttacctg gtgtttgaat atatggacca tgatctgatg ggactgctgg aatcaggctt
     3121 ggttcatttt aatgaaaacc atataaaatc ttttatgaga cagctcatgg aaggcctgga
     3181 ttattgtcat aagaagaact ttttgcatag agatattaaa tgttcaaata tccttctaaa
     3241 taatagagga cagataaaac ttgcagattt tggacttgct cgtttatata gctcagaaga
     3301 aagtcgccca tatactaaca aggtcattac tttgtggtat cgtccacctg aattgctctt
     3361 gggagaagaa cgatatacac cagccattga tgtatggagc tgtggatgta tccttggtga
     3421 actcttcact aaaaaaccta tatttcaagc aaaccaggaa cttgcacagc tagagctaat
     3481 aagccgtata tgtgggagtc catgtcctgc agtgtggcct gatgtaatca aactgccata
     3541 tttcaacacc atgaaaccaa agaagcaata tcggcggaag ttaagagaag aatttgtttt
     3601 catccccgca gctgcactcg acttattcga ttacatgctt gccttggatc ccagtaagcg
     3661 ctgcactgct gagcaggctc ttcagtgtga gttcctgcga gacgtggaac cctccaaaat
     3721 gcctccacca gaccttcctt tgtggcaaga ttgtcatgaa ttatggagta aaaagagaag
     3781 aagacagaaa cagatgggca tgactgatga tctttccaca atcaaagccc ctaggaagga
     3841 cctgtctctg ggcttagatg acagcagaac taacacaccc cagggtgtgc tgccacccgc
     3901 acagttgaaa tctcagagca actcaaatgt agcacctgta ataacaggcc ctggacagcc
     3961 gttaaaccac agtgaattgg caattcttct aaacctacta caatctaaat caagtgttaa
     4021 tatggctgat tttgtccaag tgttgaacat taaggtaaac tctgagactc aacagcagct
     4081 aaataaaata aaccttcctg ctggaatttt ggcaacaggt gaaaaacaga cagatccatc
     4141 aacaccacaa caggagtctt caaaatcatt gggaggagtt cagccttcac agaccatcca
     4201 gcctaaagtg gaaactgatg ctgcccaggc tgctgtgcag agtgcatttg cagtcctctt
     4261 gactcagtta ataaaggccc aacagtccaa acagaaagat gccatgctag aggaaaggga
     4321 aaatggatca ggacatgaag ctccattgca actcaggcct cctctagaac cgagcactcc
     4381 tggatccggg caagatgacc tcatccagca ccaagacagg aggatattgg agctgacacc
     4441 agaaccagac cggcctcgaa ttctgcctcc tgatcaacgg cctcctgaac ctcctgaacc
     4501 accaccagtt actgaggagg acctagatta tcggacagaa aaccagcatg ttcctaccac
     4561 tagttcttca ttaactgacc cacatgctgg agtgaaggca gccctcttac agctgcttgc
     4621 tcagcatcag ccccaggatg atcccaaaag agaaggtggt atcgattatc ccacaggaga
     4681 cacatatgtg cccagttcag actataagga caactttgga tcttctttct ctgccgctcc
     4741 ttacgttagc agtgatggtc taggaagtag ctccgctgct gcaccattgg aagcacgtag
     4801 tttcattgga aactcagata ttcagtctct ggataactac agtactgctt catctcacac
     4861 tggtggtcca cctcaaactt ctgcctttac tgagtcgttt gccagttcag tagctggata
     4921 tggagacatt tacctcaatg ctggtcccat gttgtttagt ggagacaagg accatagatt
     4981 tgaatatagc catggtccta tcacagtcct cacaaacagc aatgaccctt ccacagggcc
     5041 agagagtact catcccttgc cagcaaagat gcacaactat aactatggtg gtaacttaca
     5101 ggaaaatcca ggtggcccta gcctcatgca tggacagacc tggacttctc ctgcccaagg
     5161 acctggatat tcacaaggat acaggggaca cattagcaca tcagctggca gaggtcgagg
     5221 cagagggtta ccatactgac tcgagaaggg cgaattcgac ccagctttct tgtacaaagt
     5281 tggcattata aaaaataatt gctcatcaat ttgttgcaac gaacaggtca ctatcagtca
     5341 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     5401 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     5461 agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc
     5521 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     5581 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     5641 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     5701 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     5761 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     5821 tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca
     5881 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     5941 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     6001 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     6061 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     6121 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     6181 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     6241 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     6301 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     6361 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     6421 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     6481 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     6541 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     6601 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa
     6661 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6721 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6781 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6841 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6901 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6961 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7021 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7081 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7141 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7201 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7261 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7321 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
