LOCUS       sequence-246552-       15296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15296)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX044
FEATURES             Location/Qualifiers
     source          1..15296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5410..5434)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5463..5552
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5586..5838
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6267..6519)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6987..8012)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6987..8012)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8022..8366)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8508..8525)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8508..8524)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8517..8539)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8664..8683)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8874..8895
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9142..9166
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10762..11910)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12209..13003)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13195..13962
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14655..15286
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgtgtta
     4621 tctcacaatt ttaggtaatt gttacagggc tagagttgaa tcatgtgctc caacaagttc
     4681 caaagcttgc agagagaaac agcggagaga taggctaaat gacaagtgat ctcacaaccc
     4741 attatataag aacttttctc tttatttcat gatgaattgc taaaactgac ctagttatca
     4801 ggttcacgga attgggtgca ctccttgagc ctggaaggcc ccccaaaacg gacaaatctg
     4861 ctattctggt ggatgctgtt cgcatggtga cccagttacg tggtgaagct caaaagctga
     4921 aaaactcaaa tttgaatcta caagaaaaga taaaggagtt aaaggctagt attacttccc
     4981 tcgcaatgca atcctttata gaaacttttt tttcttcttg catctgtcat tctacttggt
     5041 taagggtacc aagaattttc cattttgtag tggtgacagt taaaagctag agcattactt
     5101 cactattctg ttcctgaact tcaagcatgt gttaacttgt gtaggccgag aaaaatgagc
     5161 ttcgagatga gaagcagaag cttaaggctg aaaaggaaaa gctagagcaa caactaaaaa
     5221 ctacgaatgt gcaacctggt ttcttgcctc ctgccatacc tgctgctttt gcccctcatg
     5281 gtcaagttcc aggaagcaag ctggtgccga tcatgagtta ccctggtgtt gcaatgtggc
     5341 aattcatgcc tcctgctgct gttgatacat cacaggacca cgtgctccgc cctccagttg
     5401 ctcccgggga cccagctttc ttgtacaaag tggttgataa cagcgggtta attaacatct
     5461 tttacccata cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcag
     5521 gatcctatcc atatgacgtt ccagattacg ctgctcagtg cagcgcttag agctcgaatt
     5581 tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     5641 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     5701 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     5761 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     5821 catctatgtt actagatcgg gaattagctt catcaacgca agacatgcgc acgaccgtct
     5881 gacaggagag gaatttccga cgagcacaga aaggacttgc tcttggacgt aggcctattt
     5941 ctcaggcaca tgtatcaagt gttcggacgt gggttttcga tggtgtatca gccgccgcca
     6001 actgggagat gaggaggctt tcttgggggg cagtcagcag ttcatttcac aagacagagg
     6061 aacttgtaag gagatgcact gatttatctt ggcgcaaacc agcaggacga attagtggga
     6121 atagcccgcg aatatctaag ttatgcctgt cggcatgagc agaaacttcc aattcgaaac
     6181 agtttggaga ggttgttttt gggcatacct tttgttagtc agcctctcga ttgctcatcg
     6241 tcattacaca gtaccgaagt ttgatcgatc tagtaacata gatgacaccg cgcgcgataa
     6301 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg
     6361 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac
     6421 atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt
     6481 caatcttaag aaactttatt gccaaatgtt tgaacgatct gcttcgacgc actccttctt
     6541 tactccacca tctcgtcctt attgaaaacg tgggtagcac caaaacgaat caagtcgctg
     6601 gaactgaagt taccaatcac gctggatgat ttgccagttg gattaatctt gcctttcccc
     6661 gcatgaataa tattgatgaa tgcatgcgtg aggggtattt cgattttggc aatagctgca
     6721 attgccgcga catcctccaa cgagcataat tcttcagaaa aatagcgatg ttccatgttg
     6781 tcagggcatg catgatgcac gttatgaggt gacggtgcta ggcagtattc cctcaaagtt
     6841 tcatagtcag tatcatattc atcattgcat tcctgcaaga gagaattgag acgcaatcca
     6901 cacgctgcgg caaccttccg gcgttcgtgg tctatttgct cttggacgtt gcaaacgtaa
     6961 gtgttggatc ccggtcggca tctactctat tcctttgccc tcggacgagt gctggggcgt
     7021 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     7081 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     7141 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     7201 agaccaatgc ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc
     7261 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     7321 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     7381 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     7441 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     7501 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     7561 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     7621 ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga
     7681 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag
     7741 atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc
     7801 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     7861 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     7921 ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg
     7981 acagacgtcg cggtgagttc aggctttttc atatcggggt cgtcctctcc aaatgaaatg
     8041 aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta
     8101 cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt
     8161 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat
     8221 cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt
     8281 ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg
     8341 atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga
     8401 tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt gacggatctc taggacgcgt
     8461 cgacgcgtcg acgcgtcgac gcgtcgacgc gtcctagaag ctaattcact ggccgtcgtt
     8521 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     8581 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     8641 ttgcgcagcc tgaatggcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8701 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8761 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     8821 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8881 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg
     8941 gattttgccg atttcggaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
     9001 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
     9061 gtctcactgg tgaaaagaaa aaccacccca gtacattaaa aacgtccgca atgtgttatt
     9121 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
     9181 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
     9241 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
     9301 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     9361 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaaatatc atctccctcg
     9421 cagagatccg aattatcagc cttcttattc atttctcgct taaccgtgac aggctgtcga
     9481 tcttgagaac tatgccgaca taataggaaa tcgctggata aagccgctga ggaagctgag
     9541 tggcgctatt tctttagaag tgaacgttga cgatatcaac tcccctatcc attgctcacc
     9601 gaatggtaca ggtcggggac ccgaagttcc gactgtcggc ctgatgcatc cccggctgat
     9661 cgaccccaga tctggggctg agaaagccca gtaaggaaac aactgtaggt tcgagtcgcg
     9721 agatcccccg gaaccaaagg aagtaggtta aacccgctcc gatcaggccg agccacgcca
     9781 ggccgagaac attggttcct gtaggcatcg ggattggcgg atcaaacact aaagctactg
     9841 gaacgagcag aagtcctccg gccgccagtt gccaggcggt aaaggtgagc agaggcacgg
     9901 gaggttgcca cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc cccgccaggc
     9961 ccgctgcgac gccgacagga tctagcgctg cgtttggtgt caacaccaac agcgccacgc
    10021 ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta cctagcagag
    10081 cggcagagat gaacacgacc atcagcggct gcacagcgcc taccgtcgcc gcgaccccgc
    10141 ccggcaggcg gtagaccgaa ataaacaaca agctccagaa tagcgaaata ttaagtgcgc
    10201 cgaggatgaa gatgcgcatc caccagattc ccgttggaat ctgtcggacg atcatcacga
    10261 gcaataaacc cgccggcaac gcccgcagca gcataccggc gacccctcgg cctcgctgtt
    10321 cgggctccac gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg ccgtcctcct
    10381 gtttgaagac cgacagccca atgatctcgc cgtcgatgta ggcgccgaat gccacggcat
    10441 ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg taaacggacc
    10501 cgaacatctc tggagctttc ttcagggccg acaatcggat ctcgcggaaa tcctgcacgt
    10561 cggccgctcc aagccgtcga atctgagcct taatcacaat tgtcaatttt aatcctctgt
    10621 ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg
    10681 tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg aacccccagc
    10741 cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc
    10801 gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca
    10861 cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg
    10921 gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt
    10981 gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc
    11041 ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg
    11101 gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt
    11161 cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca
    11221 gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg
    11281 cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac
    11341 gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc
    11401 ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg
    11461 catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt
    11521 gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc
    11581 caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt
    11641 catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc
    11701 cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt
    11761 agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt
    11821 gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct
    11881 gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca
    11941 cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata
    12001 atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt
    12061 ggtattccga atcttgccct gcacgaatac cagcgacccc ttgcccaaat acttgccgtg
    12121 ggcctcggcc tgagagccaa aacacttgat gcggaagaag tcggtgcgct cctgcttgtc
    12181 gccggcatcg ttgcgccaca tctaggtact aaaacaattc atccagtaaa atataatatt
    12241 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
    12301 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
    12361 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
    12421 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
    12481 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
    12541 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
    12601 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
    12661 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
    12721 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
    12781 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
    12841 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
    12901 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
    12961 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
    13021 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
    13081 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
    13141 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat tatgggtgat
    13201 gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt
    13261 ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac
    13321 cgccggacat cagcgctatc tctgctctca ctgccgtaaa acatggcaac tgcagttcac
    13381 ttacaccgct tctcaacccg gtacgcacca gaaaatcatt gatatggcca tgaatggcgt
    13441 tggatgccgg gcaacagccc gcattatggg cgttggcctc aacacgattt tacgtcactt
    13501 aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc
    13561 tgcgcggaaa tggacgaaca gtggggctat gtcggggcta aatcgcgcca gcgctggctg
    13621 ttttacgcgt atgacagtct ccggaagacg gttgttgcgc acgtattcgg tgaacgcact
    13681 atggcgacgc tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt gatatggatg
    13741 acggatggct ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt aatcagcaag
    13801 cgatatacgc agcgaattga gcggcataac ctgaatctga ggcagcacct ggcacggctg
    13861 ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc atgacaaagt catcgggcat
    13921 tatctgaaca taaaacacta tcaataagtt ggagtcatta cccaattatg atagaattta
    13981 caagctataa ggttattgtc ctgggtttca agcattagtc catgcaagtt tttatgcttt
    14041 gcccattcta tagatatatt gataagcgcg ctgcctatgc cttgccccct gaaatcctta
    14101 catacggcga tatcttctat ataaaagata tattatctta tcagtattgt caatatattc
    14161 aaggcaatct gcctcctcat cctcttcatc ctcttcgtct tggtagcttt ttaaatatgg
    14221 cgcttcatag agtaattctg taaaggtcca attctcgttt tcatacctcg gtataatctt
    14281 acctatcacc tcaaatggtt cgctgggttt atcgcacccc cgaacacgag cacggcaccc
    14341 gcgaccacta tgccaagaat gcccaaggta aaaattgccg gccccgccat gaagtccgtg
    14401 aatgccccga cggccgaagt gaagggcagg ccgccaccca ggccgccgcc ctcactgccc
    14461 ggcacctggt cgctgaatgt cgatgccagc acctgcggca cgtcaatgct tccgggcgtc
    14521 gcgctcgggc tgatcgccca tcccgttact gccccgatcc cggcaatggc aaggactgcc
    14581 agcgctgcca tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat
    14641 gggaggcccg cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt
    14701 gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt
    14761 aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat
    14821 gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc
    14881 agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct
    14941 caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact
    15001 cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg
    15061 aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc
    15121 aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg
    15181 gcggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata
    15241 gacggccgcc agcccagcgg cgagggcaac cagcccggtg agcgtcggaa aggcgc
//
