LOCUS       sequence-246559-        7299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into 
            Gateway entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Dubbury SJ, Boutz PL, Sharp PA
  TITLE     CDK12 regulates DNA repair genes by suppressing intronic 
            polyadenylation.
  JOURNAL   Nature. 2018 Dec;564(7734):141-145. doi: 10.1038/s41586-018-0758-y. 
            Epub 2018 Nov 28.
  PUBMED    30487607
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0758-y"; journalName: "Nature"; date: "2018-12";
            volume: "564"; issue: "7734"; pages: "141-145"
COMMENT     Sequence Label: pCR8/GW_TOPO_Cdk12_Untagged
FEATURES             Location/Qualifiers
     source          1..7299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5178..5277)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5294..5313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5295..5313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5318..5334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5318..5334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5765..6556
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6649..7237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7138..7157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcgccc ttactactga gctcgccgcc accatgccca attccgagag
      721 acatgggggc aagaaggacg ggagcggagg agcttctgga acttcgcagc cgtcatcggg
      781 aggtggcagc tccaacagca gggagcgtca ccgcttggtg tcgaagcaca agcggcataa
      841 gtccaagcac tccaaagacg tggggctggt gacccccgaa gcggcatctt tgggtaccat
      901 aatcaaacca ctggtggagt acgatgacat cagctctgat tcagacacct tctccgatga
      961 cacggccttc aaatcagacc ggagggagaa cgaggaacgt cggggaacgg atcggagcga
     1021 tcgcctgcac cgacatcgtc accaccagca ccggcggtcc cgagacttgc taaaaactaa
     1081 acagacggaa aaggaaaaaa atcaggaagt ctccaaatct ggatctatga aggaccgggt
     1141 atcgggcagt tccaaacggt ccgtggaggg gagtgatgat tatgggaagg cccagctatc
     1201 caaaagcggc agcaaggaat ccaggtcgtc caaaatgcac aaggagaaga cccgcaaaga
     1261 gcgagagtta aagtctggat acaaggaccg gagtaaaagt catcggaaaa gggaaacacc
     1321 caaaagttac aaaaccgtgg ctagccctaa acggagatcc aggagtcccc ataggaaatg
     1381 gtctgacagt tccaagcaag atgacagccc ttccggagct tcttatggcc aagactacga
     1441 tcttagcccc ccaaggtctc acacttctag caactatgac tcctacaaga agagtcctgg
     1501 aagtacctca agaaggcagt caatcagccc accttacaaa gagccttctg cttaccagtc
     1561 cagcactcgg tcacccagtc cttacagccg acgacagagg tctgtgagtc cctatagccg
     1621 gagacggtcc tccagctatg aaaggagcgg ctcttacagc gggagatcac ccagccccta
     1681 tggccgaagg cgatcaagca gccctttcct gagcaagagg tctctgagtc ggagtccact
     1741 ccccagtagg aaatccatga agtccagaag tagaagtcct gcatattcaa gacactcatc
     1801 ttctcatagt aaaaagaagc gatccgggtc acgcagtcgt cattccagta tctcacctgt
     1861 caggcttcca ttgaattcca gcctgggagc tgaactcagt agaaaaaaga aggaaagagc
     1921 agcagctgct gcagcagcaa aaatggatgg aaaagagtcc aagagttcac ctataatttt
     1981 gcctaaaaaa gagaaacttg aggtgaagga gtcagggtta gagtctaaaa agttacccag
     2041 aagtataaaa tcagaaaaat cgaccccaga tactgaactg gttactgtag cacattcaaa
     2101 cccagaggta aaacattgtt tagacacagg gaaggttagg ttggatgaga acttgcagaa
     2161 gcatcctgct aaggatttga aagcacaggg aacaaaggac gttaaacctg tagcaccgaa
     2221 agaggtgatt gttacttcaa aggagacaga gacatcagaa aaggagaccc ttccacctct
     2281 tcccacaatt acttctccac cccctttacc agctactacc cctccacctc agacaccccc
     2341 tttgccacct ttgcctccac taccagctat tccgctgcag ccacctctgc ctcctcccca
     2401 accaccattt agtcaagttc ctgtttcaag tacttcaatt ttaccctctt ctcctcaccc
     2461 aaggacatct actctatcct ctcagacaaa ttctcagccc cctgtacagg tttctatgaa
     2521 gactcaagta tctataacag ctgctattcc acatctgaag acttcaacat tgcctcctct
     2581 gcccctccct cccctattac ctggagatga tgacatggat agtccaaaag aaacacttcc
     2641 ttcaaagcct gcaaagaaag agaaggaaca gaggactcgc cacttgctta cagacttgcc
     2701 tcttcctcct gagctaccag gaggagatcc atcgcctcca gattctccag agccaaaggc
     2761 aattacacca cctcaacaac catataaaaa gagaccaaaa atttgttgtc cacgttatgg
     2821 agaaagaaga caaacagaaa gtgattgggg gaagcgctgt gtggacaagt ttgacattat
     2881 tgggattatt ggagagggga cctatggcca agtatataaa gccaaggaca aagacacagg
     2941 agaactagta gctctgaaga aggttcggct ggacaatgag aaagagggct tcccaatcac
     3001 agccatccgg gagatcaaga ttcttcgtca attagttcac cagagtgttg taaacatgaa
     3061 ggaaattgtc acagacaaac aagatgcact ggatttcaag aaggacaaag gtgcctttta
     3121 ccttgtattt gaatatatgg accatgactt aatgggactg cttgaatcag gtttggtgca
     3181 cttttctgag gaccatatca agtcatttat gaaacagcta atggaaggac tggattactg
     3241 tcacaaaaag aatttcctcc atcgggatat taaatgttct aacattttgc tgaataacag
     3301 cgggcaaatc aaactggcag attttggact tgctcggctc tataactctg aagagagtcg
     3361 cccttacaca aacaaagtca tcactctttg gtatcgacct ccagagcttc ttcttggaga
     3421 ggaaagatac acaccagcca ttgatgtttg gagctgtggg tgcatccttg gagaactgtt
     3481 cacaaagaaa cctatttttc aagccaattt agaactggct cagctagaac ttatcagtcg
     3541 tctctgtggt agtccttgtc cagcagtgtg gcctgatgtt atcaagctgc cctacttcaa
     3601 caccatgaaa ccgaagaagc aatacaggag acgcctaaga gaagaattct ctttcattcc
     3661 ttcagcggca cttgatctat tggaccacat gctgacactg gatcctagca agaggtgcac
     3721 agctgaacag accctacaga gtgactttct taaagatgtg gaactcagca aaatggcacc
     3781 tccagaccta cctcactggc aggattgcca tgaattgtgg agtaagaaac gtcgacggca
     3841 gcgacagagt ggtattgtga tagaagatcc acctccgtcc aaagcttcta gaaaagaaac
     3901 tacctcaggg acaacagctg agcctgtgaa aaacaatagc ccagcaccac ctcagcctgc
     3961 tcctgtcaag gcagagcctg gtccagggga tgcagtaggc cttggtgaca tcacacagca
     4021 gttgaatcaa agtgaattgg cagtgttatt aaacctgctt cagagccaaa ctgacctgag
     4081 catccctcag atggcacagc tgcttaatat ccactccaat ccagagatgc aacagcagct
     4141 tgaagccttg aatcagtcta ttagtgcact gactgaagcc agttcccagc agcaggactc
     4201 agaatccata gcccctgaag aatcattgaa ggaggtacct tctgtacctg tggtcctgcc
     4261 tcctgctgaa cagacaactc ctgaagcttc aaacacacca gctgacatgc agaatgtgtt
     4321 ggcagttctc ttgagtcagc tgatgaaaac ccaagagcca gcaggtaacc tggaggaaaa
     4381 caccaatgac aagaatagtg ggccacaggg gccccgaaga actcctacaa tgccacagga
     4441 ggaggcagca gcatgtcctc ctcacattct tccaccagag aagaggcccc ctgagccccc
     4501 tggacctcca ccgccgccac ctccaccccc tctggttgaa ggcgatcttt ccagcgcccc
     4561 ccaggagttg aatcccgccg tgacagccgc cttgctgcaa cttttatccc agcctgaagc
     4621 agagcctcct ggccacctgc cacatgagca ccaggccttg agaccaatgg aatactccac
     4681 ccgatcccat ccaaacagga cttacggaaa tactgatggg cctgagacag ggttcagttc
     4741 cgctgacact gatgaacgca gttctggtcc agccttgaca gaatctttgg ttcagacccc
     4801 ggtgaagaac aggaccttct caggctctgt gagccacctt ggggagtcca acagctacca
     4861 gggcacaggg tcagtgcagt tcccagggga ccaggacctc cgttttacca gggttccctt
     4921 agcattacac tcagtggttg ggcaaccatt cctcaagtct gagggaaata gcaactctgt
     4981 ggtacatgca gagaccaaat tgcaaaacta tggggagctg ggaccgggaa ccactggggc
     5041 caacagctca ggaacaacgc ttcagtgggg gggcccagct cagtcttatg gaaaacccta
     5101 caggggggct gcaagagtcc taccacgagg gggaagaggg agaggagttc cttattaact
     5161 cgagaagggc gaattcgacc cagctttctt gtacaaagtt ggcattataa aaaataattg
     5221 ctcatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     5281 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     5341 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     5401 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
     5461 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     5521 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     5581 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     5641 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     5701 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     5761 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     5821 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     5881 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     5941 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     6001 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     6061 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     6121 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     6181 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     6241 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     6301 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     6361 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     6421 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     6481 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     6541 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
     6601 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6661 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6721 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6781 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6841 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6901 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6961 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7021 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7081 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7141 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7201 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7261 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
