LOCUS       sequence-246564-        7431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene 
            (NP_001074527.1 isoform) cloned into Gateway entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Insco ML, Abraham BJ, Dubbury SJ, Kaltheuner IH, Dust S, Wu C, Chen 
            KY, Liu D, Bellaousov S, Cox AM, Martin BJE, Zhang T, Ludwig CG, 
            Fabo T, Modhurima R, Esgdaille DE, Henriques T, Brown KM, Chanock 
            SJ, Geyer M, Adelman K, Sharp PA, Young RA, Boutz PL, Zon LI
  TITLE     Oncogenic CDK13 mutations impede nuclear RNA surveillance.
  JOURNAL   Science. 2023 Apr 21;380(6642):eabn7625. doi: 
            10.1126/science.abn7625. Epub 2023 Apr 21.
  PUBMED    37079685
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-04-21"; volume: "380(6642):eabn7625. doi"; pages: " 
            10.1126/science.abn7625. Epub 2023 Apr 21"
COMMENT     Sequence Label: pCR8/GW_TOPO_Cdk13_Nterm_FlagHa
FEATURES             Location/Qualifiers
     source          1..7431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      698..707
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             707..730
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             731..757
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4270..4287
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     protein_bind    complement(5310..5409)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5426..5445)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5427..5445)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5450..5466)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5450..5466)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5897..6688
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6781..7369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7270..7289
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcgccc ttactactgg taccgccgcc accatggact acaaggacga
      721 cgatgacaag tacccttatg acgtgcccga ttacgctcct tccagctccg atacagctct
      781 cggcggagga ggcggactca gctgggccga gaaaaagctg gaggagagga ggaagaggag
      841 gaggtttctc agccctcagc agcctcctct gctgctccct ctcctgcaac ctcagctcct
      901 ccaaccccct cctcctcccc ctcctctcct gtttctggct gctcctggag ccgctgccgc
      961 tgccgccgct gctgctgccg cttccagctc ctgttttagc cctggccctc ctctggaggt
     1021 caagaggctg gccagaggaa aaaggagacc cggcggcagg caaaagagaa ggagaggacc
     1081 tagggccgga caggaagccg aaaagaggag agtgttctcc ctgcctcaac cccagcagga
     1141 tggaggcgga ggagcttcca gcggaggagg agtgacaccc ctggtggagt acgaggacgt
     1201 gtccagccaa agcgaacaag gcctgctcct gggaggagct agcgctgcta cagctgccac
     1261 cgctgctgga ggaaccggag gaaatggagg atcccccgct tccagcagcg gaacccagag
     1321 aagagccgag ggcagcgaga gaagacctag aagggacaga aggagctcca gcggcaggtc
     1381 caaagagagg cacagggaac ataggaggag agatggcaca aggagcggat ccgaggccag
     1441 caaggccaga tccagacacg gacacagcgg cgaggaaaga gccgaggctg ccaaatccgg
     1501 ctcctccagc tcctccggcg gaagaagaaa gtccgcctcc gccacatcct ccagcagcag
     1561 ctccaggaag gacagggacc tcaaggctca taggagcagg accaagagct ccaaggaacc
     1621 cccttccgcc tacaaggagc cccccaaagc ctacagggaa gacaagtccg aacccaaggc
     1681 ctacaggagg aggcagaggt ccctcagccc tctcggaggc agagacgagt cccctgtcag
     1741 ccacagggct agccagtccc tgagatccag gaaaagccct tcccccgctg gaggcggaag
     1801 cagcccttat tccagaagac tgcccaggtc ccctagcccc tacagcagga ggagatcccc
     1861 cagctacagc aggcattcct cctacgagag aggaggagat gtctccccct ccccctacag
     1921 ctcctcctcc tggaggagga gcagatcccc ctattcccct gtgctgagga ggtccgccaa
     1981 gtccaggtcc aggagccctt actcctccag gcacagcaga agcaggtcca ggcacaggct
     2041 gagcaggtcc agaagcaggc actcctccat cagcccctcc accctgacac tgaagagctc
     2101 cctggccgcc gagctcaaca agaacaagaa agctagggcc gctgaggctg ctagagctgc
     2161 cgaagccgct aaagccgccg aagccgccaa agctgctgaa gccgccgcca aggctgctaa
     2221 ggcctccaac gcttccacac ccaccaaggg aaacaccgag accggagcct ccgtctccca
     2281 gaccaaccac gtgaaggaag tcaagaagct taaaactgag catgcacctt ctccttcaag
     2341 tggtgggacc gtcaaaagcg acaaagcaaa aacaaagcca ccgcttcaag taacaaaggt
     2401 agacaataat ttgacagtag agaaagccac caagaaaaca gttgttggga aggagagtaa
     2461 acctgctgct acaaaggaag aaccagtttc cactaaagag aaaagcaagc cactcacacc
     2521 aagcacagga gccaaggaga aggagcagca tgtggcttta gtgacctcta cgttaccgcc
     2581 attacctttg cctcccatgc tgcctgaaga taaagatgct gatagcttaa gaggcaacat
     2641 ttctgtcaaa gcagttaaaa aagaagtaga aaagaaactc cgatgtctgc ttgctgattt
     2701 accattgccc cctgagttac caggaggaga tgatctttcc aagagtccag aggagaagaa
     2761 aacagcagca cagttacata gcaaacgaag gcctaaaata tgtgggcctc gctatggtga
     2821 aatcaaagaa aaagatattg actgggggaa acgctgcgtg gataaatttg atatcatcgg
     2881 aattattgga gaaggtactt atggacaagt ttacaaagcc agggacaaag acacgggaga
     2941 aatggtagcc ttaaagaaag tacgtctgga taatgaaaag gagggtttcc caattacagc
     3001 aattagagaa attaaaattc ttcggcaact cacccaccag agtatcatca atatgaagga
     3061 aatcgtgact gataaagaag atgctttgga ttttaagaaa gacaaaggtg cattttacct
     3121 ggtgtttgaa tatatggacc atgatctgat gggactgctg gaatcaggct tggttcattt
     3181 taatgaaaac catataaaat cttttatgag acagctcatg gaaggcctgg attattgtca
     3241 taagaagaac tttttgcata gagatattaa atgttcaaat atccttctaa ataatagagg
     3301 acagataaaa cttgcagatt ttggacttgc tcgtttatat agctcagaag aaagtcgccc
     3361 atatactaac aaggtcatta ctttgtggta tcgtccacct gaattgctct tgggagaaga
     3421 acgatataca ccagccattg atgtatggag ctgtggatgt atccttggtg aactcttcac
     3481 taaaaaacct atatttcaag caaaccagga acttgcacag ctagagctaa taagccgtat
     3541 atgtgggagt ccatgtcctg cagtgtggcc tgatgtaatc aaactgccat atttcaacac
     3601 catgaaacca aagaagcaat atcggcggaa gttaagagaa gaatttgttt tcatccccgc
     3661 agctgcactc gacttattcg attacatgct tgccttggat cccagtaagc gctgcactgc
     3721 tgagcaggct cttcagtgtg agttcctgcg agacgtggaa ccctccaaaa tgcctccacc
     3781 agaccttcct ttgtggcaag attgtcatga attatggagt aaaaagagaa gaagacagaa
     3841 acagatgggc atgactgatg atctttccac aatcaaagcc cctaggaagg acctgtctct
     3901 gggcttagat gacagcagaa ctaacacacc ccagggtgtg ctgccacccg cacagttgaa
     3961 atctcagagc aactcaaatg tagcacctgt aataacaggc cctggacagc cgttaaacca
     4021 cagtgaattg gcaattcttc taaacctact acaatctaaa tcaagtgtta atatggctga
     4081 ttttgtccaa gtgttgaaca ttaaggtaaa ctctgagact caacagcagc taaataaaat
     4141 aaaccttcct gctggaattt tggcaacagg tgaaaaacag acagatccat caacaccaca
     4201 acaggagtct tcaaaatcat tgggaggagt tcagccttca cagaccatcc agcctaaagt
     4261 ggaaactgat gctgcccagg ctgctgtgca gagtgcattt gcagtcctct tgactcagtt
     4321 aataaaggcc caacagtcca aacagaaaga tgccatgcta gaggaaaggg aaaatggatc
     4381 aggacatgaa gctccattgc aactcaggcc tcctctagaa ccgagcactc ctggatccgg
     4441 gcaagatgac ctcatccagc accaagacag gaggatattg gagctgacac cagaaccaga
     4501 ccggcctcga attctgcctc ctgatcaacg gcctcctgaa cctcctgaac caccaccagt
     4561 tactgaggag gacctagatt atcggacaga aaaccagcat gttcctacca ctagttcttc
     4621 attaactgac ccacatgctg gagtgaaggc agccctctta cagctgcttg ctcagcatca
     4681 gccccaggat gatcccaaaa gagaaggtgg tatcgattat cccacaggag acacatatgt
     4741 gcccagttca gactataagg acaactttgg atcttctttc tctgccgctc cttacgttag
     4801 cagtgatggt ctaggaagta gctccgctgc tgcaccattg gaagcacgta gtttcattgg
     4861 aaactcagat attcagtctc tggataacta cagtactgct tcatctcaca ctggtggtcc
     4921 acctcaaact tctgccttta ctgagtcgtt tgccagttca gtagctggat atggagacat
     4981 ttacctcaat gctggtccca tgttgtttag tggagacaag gaccatagat ttgaatatag
     5041 ccatggtcct atcacagtcc tcacaaacag caatgaccct tccacagggc cagagagtac
     5101 tcatcccttg ccagcaaaga tgcacaacta taactatggt ggtaacttac aggaaaatcc
     5161 aggtggccct agcctcatgc atggacagac ctggacttct cctgcccaag gacctggata
     5221 ttcacaagga tacaggggac acattagcac atcagctggc agaggtcgag gcagagggtt
     5281 accatactga ctcgagaagg gcgaattcga cccagctttc ttgtacaaag ttggcattat
     5341 aaaaaataat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     5401 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     5461 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     5521 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctacc
     5581 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     5641 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     5701 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     5761 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     5821 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     5881 aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     5941 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     6001 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     6061 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     6121 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     6181 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     6241 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     6301 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     6361 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     6421 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     6481 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     6541 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     6601 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     6661 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
     6721 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6781 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6841 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6901 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6961 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7021 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7081 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7141 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7201 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7261 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7321 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7381 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
