LOCUS       sequence-246565-        6950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human codon-optimized D. melanogaster Orco (hOrco) and Or47a 
            (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin 
            (mRho) and HCN1 (mER) for improved trafficking in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, 
            Lavista-Llanos S
  TITLE     Optimization of Insect Odorant Receptor Trafficking and Functional 
            Expression Via Transient Transfection in HEK293 Cells.
  JOURNAL   Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062.
  PUBMED    31504297
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Senses"; date: "2019-10-26- 26"; volume: "44"; issue: "9"; 
            pages: "673-682"
COMMENT     Sequence Label: pDmelOR-mRho.V5.mER.hOr47a
FEATURES             Location/Qualifiers
     source          1..6950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..66
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(270..289)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     389..411
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(449..467)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        535..639
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(640..708,709..1500)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(858..877)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1671..2259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2160..2179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2548..2629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3960..4001)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      4019..4028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        4173..4476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4477..4680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4631..4651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4714..4723
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4723..4752
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          5968..6100
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     6083..6102
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    6481..6562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6573..6590)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      join(6698..6950,1..203)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6785..6804)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
       61 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
      121 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
      181 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
      241 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
      301 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
      361 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
      421 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
      481 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      541 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      601 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
      661 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
      721 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
      781 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
      841 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
      901 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
      961 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1021 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1081 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1141 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1201 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     1261 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     1321 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     1381 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     1441 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     1501 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     1561 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     1621 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1681 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1741 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1801 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     1861 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1921 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1981 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2041 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2101 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2161 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2221 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2281 ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgacagat cttcaatatt
     2341 ggccattagc catattattc attggttata tagcataaat caatattggc tattggccat
     2401 tgcatacgtt gtatctatat cataatatgt acatttatat ttatcagggt tattgtctca
     2461 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2521 ttccccgaaa agtgccacct gacgtcggca gtgaaaaaaa tgctttattt gtgaaatttg
     2581 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     2641 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     2701 aaacctctac aaatgtggta tggctgatta tgatcctcta gactgcagcc tcaggagatc
     2761 tgggcccccg cggcatatga ccggtgtcaa gaaaagctgc gcagcatagt gatgtaggac
     2821 atggctgttc tcactatgga actgaaagcc tccatgttag cctcgaagaa gtacccggta
     2881 atccggaagc cccgatgtgc tctcatcatg ctgatgagca ggctcctgca tatggaagtg
     2941 ctggcacctc ctgcacccag agattcgtgc caaggactgt catagatcgc ccactcaaag
     3001 ctggcggatt cagtcttgag attctcccca caatagcagt atatgtaggc ctgaataatg
     3061 attgtggcga tgaaggacgc atagtagaca ccttctgtct gggcaaaggt gatgctaaac
     3121 agatagccaa gcatacacag ctggagtgag cttataaaga actgggcaca gatgatgggc
     3181 tggtagcact ggttcaggtc attgcacatg tccaatgagg tctgatacag agcgaacacc
     3241 ttgttcaaag tggcttggct ctctttcaga cttccgccct taaacctgac gactttgtac
     3301 tgggcgatct tgaagaatgc gcagaggtta ctggtaaagg agcaaaagat ggtgtccaag
     3361 ctaacggcag gcagtactac gcctgtagag gcaaaggctg accagatgaa tgacagcaca
     3421 taattccgaa tgatgtgaat gttccatgga aacagacaag gaaacggcag ttctggttca
     3481 gcatggcctg ccagccaata ggacagtccc atcctcacca ggggcagcac agagttcaaa
     3541 gcccaagcaa gcagaaagca agtacgaaac agcgtacaca tcctctgatc ctgtttgttt
     3601 gctgcattca gaatttcagc gtactcctcc ccttgctccg cctcattaga cataagcagc
     3661 cggaacttat cgatgaggcg tttaaagtct cttctcaagt acagaatcat gctgaatttg
     3721 aacagtccaa gaatcgtgat caggagtgca accatggcat cagcgagaag cagcacattc
     3781 ttccaattgt ggagcactgc agcgagaatg aacgggaata tggccgcaat agagaacacg
     3841 ttcatggctc gatagggtcg cttccacatc tcgcggtttt ctgagaacag atcaaagccc
     3901 agcagggcaa ttgtggattt ctgcacttgg aggaaagagt ccaaactaaa cttgttaacg
     3961 gtgctatcaa gccccagaag aggatttggg atgggttttc ctgctggagc gacttgatcc
     4021 atggtggcgg cctccaggcg atctgacggt tcactaaacg agctctgctt atataggcct
     4081 cccaccgtac acgccacctc gacatactcg agtagttatt aatagtaatc aattacgggg
     4141 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggctcg
     4201 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     4261 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     4321 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     4381 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     4441 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     4501 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     4561 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     4621 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     4681 ggtttagtga accgtcagat ccgctaggga tccgccacca tggaacagaa actgatctct
     4741 gaagaagacc tggctagcac aactagcatg caacccagta aatatacagg cctggtggcc
     4801 gatctcatgc ccaacataag ggcaatgaaa tacagcgggc tctttatgca caactttacg
     4861 ggagggtccg ccttcatgaa gaaagtctac agcagcgtac atctcgtttt ccttctgatg
     4921 cagttcacct tcattctggt caatatggcc cttaatgccg aagaggtgaa tgagctttct
     4981 gggaatacca tcaccacact gttcttcaca cactgcatca caaagttcat ctacctggca
     5041 gtgaaccaga agaactttta ccgtacactg aacatctgga atcaagtgaa cacccaccct
     5101 ctgtttgccg aatcagatgc cagataccac agtattgcac tcgccaagat gaggaaactc
     5161 ttctttcttg tgatgctgac aacagtggct agtgctactg cctggaccac tatcacgttc
     5221 tttggcgatt ctgtgaaaat ggtggtagac catgaaacca acagcagcat ccctgtcgag
     5281 attccaagac ttccgattaa gtccttttat ccctggaatg caagtcatgg catgttctac
     5341 atgatctcct tcgccttcca aatctactac gtgctgttta gcatgataca cagcaacctc
     5401 tgtgacgtga tgttctgctc ttggctgata tttgcctgtg aacagctcca gcatctgaaa
     5461 ggcattatga agccactgat ggaactgtca gcctcacttg acacttatcg cccaaattct
     5521 gctgctctct ttaggtcact ctcagctaat agcaaatccg agttgattca caacgaggag
     5581 aaagaccctg ggactgacat ggacatgtct ggcatttata gcagcaaagc cgactgggga
     5641 gcacagtttc gggcaccttc tacactccag tcctttggcg gtaatggagg aggaggtaat
     5701 gggctggtta acggtgctaa ccccaacggt ctgaccaaga aacaggagat gatggtgcga
     5761 tctgcgatca agtactgggt cgaacgccat aagcacgtgg ttagactggt tgctgctata
     5821 ggcgatacct acggtgccgc tttgctgttg catatgctga ctagcacgat caagttgact
     5881 ctgttggcat atcaagccac caagatcaat ggagtgaacg tatatgcgtt tacggttgtg
     5941 gggtatctgg gctatgctct ggcccaggta agtatcaagg ttacaagaca ggtttaagga
     6001 gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct
     6061 attggtctta ctgacatcca ctttgccttt ctctccacag gtgtttcact tttgcatttt
     6121 cggcaatcgg ctgatagagg agagttcctc tgttatggag gcagcgtaca gttgccactg
     6181 gtacgatgga agcgaagaag ccaagacttt cgttcagatt gtgtgtcagc agtgtcagaa
     6241 ggctatgtcc atctcagggg cgaaattctt caccgtgtcc ttggatctgt ttgcctccgt
     6301 actgggcgca gtcgtcacct atttcatggt cctggtccaa ctgaagtgaa agcttgtcga
     6361 cgatatctcc agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     6421 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     6481 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6541 aataaagcat ttttttcact gccccgagct tcctcgctca ctgactcgct gcgctcggtc
     6601 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccagatcg
     6661 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     6721 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6781 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6841 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6901 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
//
