LOCUS       sequence-246574-       16436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16436)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX048
FEATURES             Location/Qualifiers
     source          1..16436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6582..6606)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6635..6724
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6758..7010
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7439..7691)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8159..9184)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8159..9184)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9194..9538)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9648..9665)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9648..9664)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9657..9679)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9804..9823)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10014..10035
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10282..10306
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11902..13050)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13349..14143)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14335..15102
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15795..16426
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg cctctctctg agttcttgaa
     4621 aatggctaga gggaagcttg aatctggcca ggaaaaaagg tcatccacaa ctgatccgtc
     4681 ctctagtctt gaggatgaat tggtagagct agtatgggaa aatggtcaaa ttgtgatgca
     4741 aagtcagtcc agcagagcta agaagagccc tccttttagt attcatacat caagctcttc
     4801 gagagttaaa gacaagttca ctgggaattc ctcaaccaca aatatcggaa aatttggtgt
     4861 aatggactct atgtttgata atatgccatt atctgcgccc aatgatgaaa tggagttgac
     4921 ggaagaggat gaagtggtgc cttggttgag ttattctgca aatgatagcc cacaacaaga
     4981 ttataactct ccactgtttc cagaaatatc tggtctgatg gtcaacgaac catctggtaa
     5041 gagaggattt gcatcaatgg ataagagagg tatttctaat cagatgaatg gggcttcaca
     5101 tagtgttccc attcgtaaca ttgtgaactc agaacaagga aatgcatcaa agctctctcc
     5161 ttctacattt aacctgttat gttcaggggc accaaagaaa tcttcgccac caattcttcc
     5221 cctaagatca cgagtcttgg atattattag cagtaattct agctgcaata tgccagtttc
     5281 cgcttttgga tattcaaatt caagtcaagc ttcagctggt gttatcaaaa gcatgaagat
     5341 gcaaaagcaa aatatacctg gaaatagctc cagtttgctg aactttgcgc ctttctcaag
     5401 acctattact gggggttcag ctgtttcagg ctcgtctacc ataggggcaa ggggagctat
     5461 agagagagag aatgggggaa gtagcagaaa tcgtgttagt gaggtaccat ttgaatcata
     5521 cttcagttca gaaaaggaaa atgatgtcca ttctccacct tatatggcat cttccaaagt
     5581 cgagtcaaaa ggatctgtcg gccttcacaa gatggcatgt cctattgaac agtctgataa
     5641 tttatgcgga gatagtccaa gtaataatga catgtctcgt gatcaattta cttgttcaaa
     5701 tgcaatcaaa gaatttcctg atggcgagaa aaatgttgaa cctgtagttg tgtgttcatc
     5761 tgtatgttct ggtagtagcg cagagagagc atcaagtgat catcctcata ctttgaagag
     5821 gaaaactcgt gacaatgagg agtctgaatt ccaaagtgaa gatattgaag aagaatctgt
     5881 tggtatcaaa agtgcttatc ctgttcgagg aggtgcaggt tcaaagagaa gcagagctgc
     5941 agaagtgcat aatctgtctg agcggaggcg aagggatagg attaatgaga agatgcgcgc
     6001 attacaggat ctcataccaa actgcaataa ggtggataaa gcctcaatgc tcgatgaagc
     6061 tattgaatac ttgaagacac ttcaagttca agtacagatg atgtctatgg gagctgggtt
     6121 gtgtgcgcca ccaatgatgt ttcctgcagg aattcaatac atgcatgccg ccaatgtgcc
     6181 acaattttct ccgatgggtt tagggatggg aatgggtttt gggatgggta aacttgagat
     6241 gaatggtgga tctcctggct gctctattta tccaataccc actatgcaag gagcacatct
     6301 tcgctcccca ccagtttctg gctccgctac ttttccagga gtagctggat ctaaccttca
     6361 ctcttttagg catcctggtc aaggacttcc tgtgtcggta tcacctgcat ctctgtttcc
     6421 tatggcaggg caaccatcaa caaatgcagc tgtgtgttta aatatcacaa ctgcaggagt
     6481 tcatacagac atacggaata cttccaactt tagaccccga aaccaaggac acactaagaa
     6541 ctcaaatata atgaataatg ctgatccgag cagccccggg gacccagctt tcttgtacaa
     6601 agtggttgat aacagcgggt taattaacat cttttaccca tacgatgttc ctgactatgc
     6661 gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg ttccagatta
     6721 cgctgctcag tgcagcgctt agagctcgaa tttccccgat cgttcaaaca tttggcaata
     6781 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     6841 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     6901 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     6961 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattagc
     7021 ttcatcaacg caagacatgc gcacgaccgt ctgacaggag aggaatttcc gacgagcaca
     7081 gaaaggactt gctcttggac gtaggcctat ttctcaggca catgtatcaa gtgttcggac
     7141 gtgggttttc gatggtgtat cagccgccgc caactgggag atgaggaggc tttcttgggg
     7201 ggcagtcagc agttcatttc acaagacaga ggaacttgta aggagatgca ctgatttatc
     7261 ttggcgcaaa ccagcaggac gaattagtgg gaatagcccg cgaatatcta agttatgcct
     7321 gtcggcatga gcagaaactt ccaattcgaa acagtttgga gaggttgttt ttgggcatac
     7381 cttttgttag tcagcctctc gattgctcat cgtcattaca cagtaccgaa gtttgatcga
     7441 tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg
     7501 ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata
     7561 aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat
     7621 atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg
     7681 tttgaacgat ctgcttcgac gcactccttc tttactccac catctcgtcc ttattgaaaa
     7741 cgtgggtagc accaaaacga atcaagtcgc tggaactgaa gttaccaatc acgctggatg
     7801 atttgccagt tggattaatc ttgcctttcc ccgcatgaat aatattgatg aatgcatgcg
     7861 tgaggggtat ttcgattttg gcaatagctg caattgccgc gacatcctcc aacgagcata
     7921 attcttcaga aaaatagcga tgttccatgt tgtcagggca tgcatgatgc acgttatgag
     7981 gtgacggtgc taggcagtat tccctcaaag tttcatagtc agtatcatat tcatcattgc
     8041 attcctgcaa gagagaattg agacgcaatc cacacgctgc ggcaaccttc cggcgttcgt
     8101 ggtctatttg ctcttggacg ttgcaaacgt aagtgttgga tcccggtcgg catctactct
     8161 attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
     8221 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
     8281 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
     8341 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
     8401 gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
     8461 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
     8521 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
     8581 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
     8641 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
     8701 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
     8761 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
     8821 tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt
     8881 cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt
     8941 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
     9001 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
     9061 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
     9121 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
     9181 tcatatcggg gtcgtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
     9241 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
     9301 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
     9361 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
     9421 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
     9481 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
     9541 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
     9601 ctccaccatg ttgacggatc tctaggacgc gtcctagaag ctaattcact ggccgtcgtt
     9661 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     9721 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     9781 ttgcgcagcc tgaatggcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     9841 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     9901 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     9961 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    10021 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg
    10081 gattttgccg atttcggaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
    10141 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
    10201 gtctcactgg tgaaaagaaa aaccacccca gtacattaaa aacgtccgca atgtgttatt
    10261 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
    10321 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
    10381 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
    10441 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
    10501 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaaatatc atctccctcg
    10561 cagagatccg aattatcagc cttcttattc atttctcgct taaccgtgac aggctgtcga
    10621 tcttgagaac tatgccgaca taataggaaa tcgctggata aagccgctga ggaagctgag
    10681 tggcgctatt tctttagaag tgaacgttga cgatatcaac tcccctatcc attgctcacc
    10741 gaatggtaca ggtcggggac ccgaagttcc gactgtcggc ctgatgcatc cccggctgat
    10801 cgaccccaga tctggggctg agaaagccca gtaaggaaac aactgtaggt tcgagtcgcg
    10861 agatcccccg gaaccaaagg aagtaggtta aacccgctcc gatcaggccg agccacgcca
    10921 ggccgagaac attggttcct gtaggcatcg ggattggcgg atcaaacact aaagctactg
    10981 gaacgagcag aagtcctccg gccgccagtt gccaggcggt aaaggtgagc agaggcacgg
    11041 gaggttgcca cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc cccgccaggc
    11101 ccgctgcgac gccgacagga tctagcgctg cgtttggtgt caacaccaac agcgccacgc
    11161 ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta cctagcagag
    11221 cggcagagat gaacacgacc atcagcggct gcacagcgcc taccgtcgcc gcgaccccgc
    11281 ccggcaggcg gtagaccgaa ataaacaaca agctccagaa tagcgaaata ttaagtgcgc
    11341 cgaggatgaa gatgcgcatc caccagattc ccgttggaat ctgtcggacg atcatcacga
    11401 gcaataaacc cgccggcaac gcccgcagca gcataccggc gacccctcgg cctcgctgtt
    11461 cgggctccac gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg ccgtcctcct
    11521 gtttgaagac cgacagccca atgatctcgc cgtcgatgta ggcgccgaat gccacggcat
    11581 ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg taaacggacc
    11641 cgaacatctc tggagctttc ttcagggccg acaatcggat ctcgcggaaa tcctgcacgt
    11701 cggccgctcc aagccgtcga atctgagcct taatcacaat tgtcaatttt aatcctctgt
    11761 ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg
    11821 tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg aacccccagc
    11881 cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc
    11941 gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca
    12001 cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg
    12061 gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt
    12121 gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc
    12181 ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg
    12241 gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt
    12301 cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca
    12361 gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg
    12421 cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac
    12481 gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc
    12541 ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg
    12601 catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt
    12661 gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc
    12721 caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt
    12781 catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc
    12841 cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt
    12901 agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt
    12961 gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct
    13021 gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca
    13081 cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata
    13141 atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt
    13201 ggtattccga atcttgccct gcacgaatac cagcgacccc ttgcccaaat acttgccgtg
    13261 ggcctcggcc tgagagccaa aacacttgat gcggaagaag tcggtgcgct cctgcttgtc
    13321 gccggcatcg ttgcgccaca tctaggtact aaaacaattc atccagtaaa atataatatt
    13381 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
    13441 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
    13501 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
    13561 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
    13621 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
    13681 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
    13741 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
    13801 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
    13861 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
    13921 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
    13981 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
    14041 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
    14101 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
    14161 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
    14221 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
    14281 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat tatgggtgat
    14341 gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt
    14401 ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac
    14461 cgccggacat cagcgctatc tctgctctca ctgccgtaaa acatggcaac tgcagttcac
    14521 ttacaccgct tctcaacccg gtacgcacca gaaaatcatt gatatggcca tgaatggcgt
    14581 tggatgccgg gcaacagccc gcattatggg cgttggcctc aacacgattt tacgtcactt
    14641 aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc
    14701 tgcgcggaaa tggacgaaca gtggggctat gtcggggcta aatcgcgcca gcgctggctg
    14761 ttttacgcgt atgacagtct ccggaagacg gttgttgcgc acgtattcgg tgaacgcact
    14821 atggcgacgc tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt gatatggatg
    14881 acggatggct ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt aatcagcaag
    14941 cgatatacgc agcgaattga gcggcataac ctgaatctga ggcagcacct ggcacggctg
    15001 ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc atgacaaagt catcgggcat
    15061 tatctgaaca taaaacacta tcaataagtt ggagtcatta cccaattatg atagaattta
    15121 caagctataa ggttattgtc ctgggtttca agcattagtc catgcaagtt tttatgcttt
    15181 gcccattcta tagatatatt gataagcgcg ctgcctatgc cttgccccct gaaatcctta
    15241 catacggcga tatcttctat ataaaagata tattatctta tcagtattgt caatatattc
    15301 aaggcaatct gcctcctcat cctcttcatc ctcttcgtct tggtagcttt ttaaatatgg
    15361 cgcttcatag agtaattctg taaaggtcca attctcgttt tcatacctcg gtataatctt
    15421 acctatcacc tcaaatggtt cgctgggttt atcgcacccc cgaacacgag cacggcaccc
    15481 gcgaccacta tgccaagaat gcccaaggta aaaattgccg gccccgccat gaagtccgtg
    15541 aatgccccga cggccgaagt gaagggcagg ccgccaccca ggccgccgcc ctcactgccc
    15601 ggcacctggt cgctgaatgt cgatgccagc acctgcggca cgtcaatgct tccgggcgtc
    15661 gcgctcgggc tgatcgccca tcccgttact gccccgatcc cggcaatggc aaggactgcc
    15721 agcgctgcca tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat
    15781 gggaggcccg cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt
    15841 gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt
    15901 aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat
    15961 gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc
    16021 agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct
    16081 caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact
    16141 cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg
    16201 aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc
    16261 aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg
    16321 gcggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata
    16381 gacggccgcc agcccagcgg cgagggcaac cagcccggtg agcgtcggaa aggcgc
//
