LOCUS       sequence-246579-       14941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14941)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 14941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX050
FEATURES             Location/Qualifiers
     source          1..14941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5055..5079)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5108..5197
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5231..5483
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(5912..6164)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6632..7657)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6632..7657)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7667..8011)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8153..8170)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8153..8169)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8162..8184)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8309..8328)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8519..8540
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8787..8811
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10407..11555)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11854..12648)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12840..13607
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14300..14931
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggcttc
     4621 cacccagcaa gcggtgagtt ctgcttctga tgcagaccag cagtatgcaa agcttgacga
     4681 aaggaaaagg aagagaatgg aatccaatcg tgagtctgct cgtaggtcac ggatgaggaa
     4741 gcaacaacta ttgggagagt tgatgagcga aacaacacaa ctacataaac agaacagtat
     4801 ctgccgtgag aggattgatt ctgtcgagag aaattatcgt gcgatggatg cagagaacaa
     4861 tgtgttgaga gctcagattg ctgaattaac tgaacgtttg aattcattga actcgctcac
     4921 tcaattttgg gctgatgcta atggatttcc tgtggaactc cctgaaattc ccgacacatt
     4981 gcttgagcca tggaagttgc cttgccctat tcaacctatc gatgcctctt ctggtatgtt
     5041 gctgtttccc ggggacccag ctttcttgta caaagtggtt gataacagcg ggttaattaa
     5101 catcttttac ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta
     5161 tgcaggatcc tatccatatg acgttccaga ttacgctgct cagtgcagcg cttagagctc
     5221 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     5281 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     5341 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     5401 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     5461 ggtgtcatct atgttactag atcgggaatt agcttcatca acgcaagaca tgcgcacgac
     5521 cgtctgacag gagaggaatt tccgacgagc acagaaagga cttgctcttg gacgtaggcc
     5581 tatttctcag gcacatgtat caagtgttcg gacgtgggtt ttcgatggtg tatcagccgc
     5641 cgccaactgg gagatgagga ggctttcttg gggggcagtc agcagttcat ttcacaagac
     5701 agaggaactt gtaaggagat gcactgattt atcttggcgc aaaccagcag gacgaattag
     5761 tgggaatagc ccgcgaatat ctaagttatg cctgtcggca tgagcagaaa cttccaattc
     5821 gaaacagttt ggagaggttg tttttgggca taccttttgt tagtcagcct ctcgattgct
     5881 catcgtcatt acacagtacc gaagtttgat cgatctagta acatagatga caccgcgcgc
     5941 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat
     6001 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt
     6061 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca
     6121 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatctgcttc gacgcactcc
     6181 ttctttactc caccatctcg tccttattga aaacgtgggt agcaccaaaa cgaatcaagt
     6241 cgctggaact gaagttacca atcacgctgg atgatttgcc agttggatta atcttgcctt
     6301 tccccgcatg aataatattg atgaatgcat gcgtgagggg tatttcgatt ttggcaatag
     6361 ctgcaattgc cgcgacatcc tccaacgagc ataattcttc agaaaaatag cgatgttcca
     6421 tgttgtcagg gcatgcatga tgcacgttat gaggtgacgg tgctaggcag tattccctca
     6481 aagtttcata gtcagtatca tattcatcat tgcattcctg caagagagaa ttgagacgca
     6541 atccacacgc tgcggcaacc ttccggcgtt cgtggtctat ttgctcttgg acgttgcaaa
     6601 cgtaagtgtt ggatcccggt cggcatctac tctattcctt tgccctcgga cgagtgctgg
     6661 ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc
     6721 ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc
     6781 atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt
     6841 ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat
     6901 gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga
     6961 agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg
     7021 ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt
     7081 gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg
     7141 acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg
     7201 cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga
     7261 acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct
     7321 gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc
     7381 gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg
     7441 ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc
     7501 agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt
     7561 cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact
     7621 tctcgacaga cgtcgcggtg agttcaggct ttttcatatc ggggtcgtcc tctccaaatg
     7681 aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc
     7741 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     7801 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     7861 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     7921 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     7981 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     8041 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttgacgg atctctagga
     8101 cgcgtcgacg cgtcgacgcg tcgacgcgtc gacgcgtcct agaagctaat tcactggccg
     8161 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     8221 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     8281 aacagttgcg cagcctgaat ggcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8341 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8401 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     8461 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8521 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
     8581 taagggattt tgccgatttc ggaaccacca tcaaacagga ttttcgcctg ctggggcaaa
     8641 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     8701 tgcccgtctc actggtgaaa agaaaaacca ccccagtaca ttaaaaacgt ccgcaatgtg
     8761 ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc
     8821 caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca
     8881 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
     8941 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
     9001 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa atatcatctc
     9061 cctcgcagag atccgaatta tcagccttct tattcatttc tcgcttaacc gtgacaggct
     9121 gtcgatcttg agaactatgc cgacataata ggaaatcgct ggataaagcc gctgaggaag
     9181 ctgagtggcg ctatttcttt agaagtgaac gttgacgata tcaactcccc tatccattgc
     9241 tcaccgaatg gtacaggtcg gggacccgaa gttccgactg tcggcctgat gcatccccgg
     9301 ctgatcgacc ccagatctgg ggctgagaaa gcccagtaag gaaacaactg taggttcgag
     9361 tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc gctccgatca ggccgagcca
     9421 cgccaggccg agaacattgg ttcctgtagg catcgggatt ggcggatcaa acactaaagc
     9481 tactggaacg agcagaagtc ctccggccgc cagttgccag gcggtaaagg tgagcagagg
     9541 cacgggaggt tgccacttgc gggtcagcac ggttccgaac gccatggaaa ccgcccccgc
     9601 caggcccgct gcgacgccga caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc
     9661 cacgcccgca gttccgcaaa tagcccccag gaccgccatc aatcgtatcg ggctacctag
     9721 cagagcggca gagatgaaca cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac
     9781 cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg aaatattaag
     9841 tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc ggacgatcat
     9901 cacgagcaat aaacccgccg gcaacgcccg cagcagcata ccggcgaccc ctcggcctcg
     9961 ctgttcgggc tccacgaaaa cgccggacag atgcgccttg tgagcgtcct tggggccgtc
    10021 ctcctgtttg aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc cgaatgccac
    10081 ggcatctcgc aaccgttcag cgaacgcctc catgggcttt ttctcctcgt gctcgtaaac
    10141 ggacccgaac atctctggag ctttcttcag ggccgacaat cggatctcgc ggaaatcctg
    10201 cacgtcggcc gctccaagcc gtcgaatctg agccttaatc acaattgtca attttaatcc
    10261 tctgtttatc ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa
    10321 gtgcgtcgag cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc
    10381 ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc
    10441 caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg
    10501 cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg
    10561 agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac
    10621 agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg
    10681 atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg
    10741 aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc
    10801 gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc
    10861 gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg
    10921 gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc
    10981 tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc
    11041 agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg
    11101 tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt
    11161 tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt
    11221 tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg
    11281 atggtcatcg acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg
    11341 gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg
    11401 gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg
    11461 acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct
    11521 tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg
    11581 actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc
    11641 agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg
    11701 tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc caaatacttg
    11761 ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc
    11821 ttgtcgccgg catcgttgcg ccacatctag gtactaaaac aattcatcca gtaaaatata
    11881 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
    11941 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
    12001 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
    12061 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
    12121 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
    12181 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
    12241 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
    12301 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
    12361 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
    12421 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
    12481 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
    12541 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
    12601 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
    12661 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
    12721 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
    12781 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatgg
    12841 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
    12901 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
    12961 agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
    13021 ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
    13081 ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt
    13141 cacttaaaaa actcaggccg cagtcggtaa cctcgcgcat acagccgggc agtgacgtca
    13201 tcgtctgcgc ggaaatggac gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct
    13261 ggctgtttta cgcgtatgac agtctccgga agacggttgt tgcgcacgta ttcggtgaac
    13321 gcactatggc gacgctgggg cgtcttatga gcctgctgtc accctttgac gtggtgatat
    13381 ggatgacgga tggctggccg ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca
    13441 gcaagcgata tacgcagcga attgagcggc ataacctgaa tctgaggcag cacctggcac
    13501 ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg
    13561 ggcattatct gaacataaaa cactatcaat aagttggagt cattacccaa ttatgataga
    13621 atttacaagc tataaggtta ttgtcctggg tttcaagcat tagtccatgc aagtttttat
    13681 gctttgccca ttctatagat atattgataa gcgcgctgcc tatgccttgc cccctgaaat
    13741 ccttacatac ggcgatatct tctatataaa agatatatta tcttatcagt attgtcaata
    13801 tattcaaggc aatctgcctc ctcatcctct tcatcctctt cgtcttggta gctttttaaa
    13861 tatggcgctt catagagtaa ttctgtaaag gtccaattct cgttttcata cctcggtata
    13921 atcttaccta tcacctcaaa tggttcgctg ggtttatcgc acccccgaac acgagcacgg
    13981 cacccgcgac cactatgcca agaatgccca aggtaaaaat tgccggcccc gccatgaagt
    14041 ccgtgaatgc cccgacggcc gaagtgaagg gcaggccgcc acccaggccg ccgccctcac
    14101 tgcccggcac ctggtcgctg aatgtcgatg ccagcacctg cggcacgtca atgcttccgg
    14161 gcgtcgcgct cgggctgatc gcccatcccg ttactgcccc gatcccggca atggcaagga
    14221 ctgccagcgc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg
    14281 gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc
    14341 ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt
    14401 ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg
    14461 caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat
    14521 ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg
    14581 cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg
    14641 aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc
    14701 ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa
    14761 gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa
    14821 cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg
    14881 ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt cggaaaggcg
    14941 c
//
