LOCUS       sequence-246582-       16864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16864)
  AUTHORS   Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang
            Y, Cai M
  TITLE     Engineered ethanol-driven biosynthetic system for improving 
            production of acetyl-CoA derived drugs in Crabtree-negative yeast.
  JOURNAL   Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. 
            Epub 2019 May 9.
  PUBMED    31077813
REFERENCE   2  (bases 1 to 16864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-07"; volume: "54:275-284. doi"; pages: " 
            10.1016/j.ymben.2019.05.001. Epub 2019 May 9"
COMMENT     Sequence Label: pK_P-GAP-LM_lacO-cP-AOX1-sAR
FEATURES             Location/Qualifiers
     source          1..16864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     156..174
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    309..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2098..2115
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2195..2441
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     protein_bind    2457..2473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4747..4764
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4844..5090
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        5091..5567
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             9062..9091
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9107..9124
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      9204..9450
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(9869..12403)
                     /codon_start=1
                     /gene="Pichia pastoris HIS4"
                     /product="multifunctional enzyme, required for histidine 
                     biosynthesis"
                     /label=PpHIS4
                     /note="auxotrophic marker for Pichia pastoris"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     CDS             complement(12816..13631)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(12816..13631)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     13539..13558
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     13623..13642
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(13796..13815)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    14010..14758
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     primer_bind     complement(14794..14816)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14902..15042
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14957..14976
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15057..15074)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15228..15816)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15308..15327)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15987..16778,16779..16847))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16610..16629
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(16848..16864,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
      121 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
      181 gtcttcaaga attaattctc atgtttgaca gcttatcatc gataagctga ctcatgttgg
      241 tattgtgaaa tagacgcaga tcgggaacac tgaaaaataa cagttattat tcggagctct
      301 gtgtggaatt gtgagcggat aacaatttca cacactaacc cctacttgac agcaatatat
      361 aaacagaagg aagctgccct gtcttaaacc ttttttttta tcatcattat tagcttactt
      421 tcataattgc gactggttcc aattgacaag cttttgattt taacgacttt taacgacaac
      481 ttgagaagat caaaaaacaa ctaattattc gaaatgactg ttgacgcttt gactcagcca
      541 caccacttgt tgtccttggc ctggaacgat actcaacagc acggttcctg gtttgcccct
      601 cttgtcacta cctccgccgg tttgttgtgt ttgttgttgt acctttgttc ctccggtaga
      661 agatccgact tgcctgtctt caatcctaag acctggtggg agttgaccac tatgagagct
      721 aagcgtgatt tcgatgctaa cgccccatcc tggattgagt cctggttctc ccagaacgac
      781 aagccaatca gatttattgt tgactctggt tactgcacca tcttgccttc ctctatggct
      841 gacgagttcc gtaagatgaa ggagttgtgc atgtacaagt tcttgggtac tgacttccac
      901 tcccaccttc caggttttga tggatttaaa gaggtcacca gagacgccca tttgatcacc
      961 aaggttgtca tgaaccagtt ccagacccag gccccaaagt atgtcaagcc attggccaac
     1021 gaggcctccg gaattatcac cgacatcttc ggtgactcta acgagtggca taccgtccca
     1081 gtctacaacc agtgcttgga cttggtcact cgtaccgtca ccttcattat ggtcggatct
     1141 aagttggctc acaacgaaga gtggttggac atcgccaagc atcacgccgt taccatggct
     1201 atccaggcca gacagttgcg tttgtggcca gtcattttgc gtccattggt tcactggctt
     1261 gaacctcaag gagccaagtt gagagctcaa gttagaagag ctagacaatt gttggatcca
     1321 attattcaag aacgtagagc tgagcgtgat gcttgcagag ctaagggtat tgagccacct
     1381 agatacgtcg attccattca gtggttcgag gacactgcca agggaaaatg gtacgacgct
     1441 gctggagctc aattggctat ggatttcgcc ggaatctacg gtacctctga cttgttgatc
     1501 ggtggattgg ttgatatcgt tcgtcaccct cacttgttgg agccattgcg tgacgagatc
     1561 cgtaccgtta ttggtcaggg tggttggacc ccagcctctt tgtacaagtt gaagttgctt
     1621 gactcctgtc ttaaggagtc tcagcgtgtt aagccagtcg agtgtgccac catgagatct
     1681 tacgctttgc aggacgtcac cttctccaac ggtaccttca tccctaaggg agaattggtc
     1741 gccgttgccg ccgacagaat gtccaatcct gaggtctggc cagaaccagc caagtacgac
     1801 ccttaccgtt acatgagatt gcgtgaggac ccagctaagg ccttctctgc ccagttggag
     1861 aacaccaacg gtgaccatat cggtttcggt tggcatccta gagcttgtcc tggtcgtttc
     1921 ttcgcctcta aggagatcaa aatgatgctt gcctatttgt tgattagata cgactggaag
     1981 gtcgtcccag acgaaccatt gcagtactac agacactcct tttccgtccg tatccatcct
     2041 accaccaagt tgatgatgcg tcgtcgtgac gaggacatta gattgccagg ttctttgcat
     2101 catcatcatc atcattgagt ttgtagcctt agacatgact gttcctcagt tcaagttggg
     2161 cacttacgag aagaccggtc ttgctagatt ctaatcaaga ggatgtcaga atgccatttg
     2221 cctgagagat gcaggcttca tttttgatac ttttttattt gtaacctata tagtatagga
     2281 ttttttttgt cattttgttt cttctcgtac gagcttgctc ctgatcagcc tatctcgcag
     2341 ctgatgaata tcttgtggta ggggtttggg aaaatcattc gagtttgatg tttttcttgg
     2401 tatttcccac tcctcttcag agtacagaag attaagtgag aagatcttgt gtggaattgt
     2461 gagcggataa caatttcaca cactaacccc tacttgacag caatatataa acagaaggaa
     2521 gctgccctgt cttaaacctt tttttttatc atcattatta gcttactttc ataattgcga
     2581 ctggttccaa ttgacaagct tttgatttta acgactttta acgacaactt gagaagatca
     2641 aaaaacaact aattattcga aatggctcaa ctcgacactc tcgacctggt ggtcctggtg
     2701 gtgcttttgg tgggtagcgc cgcctacttc accaagggca cctactgggc cgttcccaag
     2761 gacccgtatg ccgcctccgg tcccgccatg aatggtggcg ccaaggcggg caaatccagg
     2821 gacatcattg agaaaatgga agagactggc aagaactgtg tgattttcta cggctcgcag
     2881 accggtaccg ccgaggatta tgcgtcgcgc ctggccaagg aaggctccca gcgtttcggc
     2941 ctcaagacca tggtcgcaga tctggaagac tacgattatg agaacctgga caagttcccc
     3001 gaggacaagg ttgccttctt cgtcatggcc acctatggtg agggtgaacc caccgacaac
     3061 gccgtcgagt tctaccagtt catctcgggt gaggacgtcg cgttcgagag cggcgcctct
     3121 gccgacgaca agcccctgtc ctccctcaag tatgtcactt tcggtctcgg taacaacacc
     3181 tatgagcact atcaggctat ggttcgcaat ctggatgccg ctctcaccaa gctgggtgcg
     3241 cagcgcattg gagatgctgg tgaaggtgat gacggcgctg gcaccatgga agaagatttc
     3301 ctggcctgga aagagcccat gtggactgcc ctgtccgagg ccatgaacct tcaggagcgc
     3361 gaagccgtct atgagccggt gttctcggtc acggaagatg aatccctgtc ccccgaagac
     3421 gaagccgtct acctcggtga gccgaccaag ggtcaccgtg acggcacccc cagtggcccg
     3481 tattccgctc acaacccctt catcgccccc atcgtcgagt ctcgtgaact gttcaacgtc
     3541 aaggaccgta attgtctgca catggagatc agcatcgctg gtagcaacct ttcttaccag
     3601 actggtgatc acatcgcgat ttggcccacg aacgctggtg ccgaggtgga ccggttcctc
     3661 caggtgtttg gtcttgagaa caagcgtcat tccgtcatca acatcaaggg tatcgatgtg
     3721 accgccaagg ttcccattcc gactcccacc acgtatgatg ctgctgttcg ctactatatg
     3781 gaaattgctg cgcccgtctc ccgtcagttt gtggctaccc tggctgcgtt tgctcccgat
     3841 gaggagacta aggcggaaat cgtgcgtttg ggtagcgaca aggactactt ccacgagaaa
     3901 atcagcaacc agtgcttcac catcgctcag gctcttcaga gtgtcacctc caagcccttc
     3961 tcggctgtcc cgttctctct gcttatcgag ggtctcaata agctccagcc ccgttactac
     4021 tccatctctt cctcctccat ggtccagaag gataagatca gcattactgc cgtcgtggaa
     4081 tccactcgct tgcctggtgc cgcccacctt gtcaagggtg tcacgaccaa ctatctcctt
     4141 gccctgaagc aaaagcagaa tggggatccg tctcccgacc ctcacggctt aacttatact
     4201 atcactgggc cccgtaacaa gtacgacgga atccacgttc ccgttcacgt ccgccactcc
     4261 aatttcaagc tcccctctga tccctctcgg cccattatca tggttggccc tggtaccggt
     4321 gtggctccct tccgtggatt catccaggag cgtgccgcct tggccgccaa gggtgagaac
     4381 gtcggtccca ccgtgttgtt ctttggatgc cgcaggcgcg atgaggactt tatgtacgca
     4441 gatgaattca agacctacca ggaacagctt ggggacaagc ttcagatcat tactgcgttt
     4501 tctcgtgaaa cttcccagaa ggtgtatgtt cagcacagac tgcgtgaaca ctccgatctg
     4561 gtgagcagcc tcctgaagca gaaggctaac ttttacgtct gcggtgacgc cgccaacatg
     4621 gcgcgtgaag tcaaccttgt gcttggccag atcatcgcgc aacagcgggg tctcccggct
     4681 gaacgggccg aggaaatggt gaagcacatg cgcagcagcg gcagctacca ggaggacgtg
     4741 tggtcacatc atcatcatca tcattgagtt tgtagcctta gacatgactg ttcctcagtt
     4801 caagttgggc acttacgaga agaccggtct tgctagattc taatcaagag gatgtcagaa
     4861 tgccatttgc ctgagagatg caggcttcat ttttgatact tttttatttg taacctatat
     4921 agtataggat tttttttgtc attttgtttc ttctcgtacg agcttgctcc tgatcagcct
     4981 atctcgcagc tgatgaatat cttgtggtag gggtttggga aaatcattcg agtttgatgt
     5041 ttttcttggt atttcccact cctcttcaga gtacagaaga ttaagtgaga tttttgtaga
     5101 aatgtcttgg tgtcctcgtc caatcaggta gccatctctg aaatatctgg ctccgttgca
     5161 actccgaacg acctgctggc aacgtaaaat tctccggggt aaaacttaaa tgtggagtaa
     5221 tggaaccaga aacgtctctt cccttctctc tccttccacc gcccgttacc gtccctagga
     5281 aattttactc tgctggagag cttcttctac ggcccccttg cagcaatgct cttcccagca
     5341 ttacgttgcg ggtaaaacgg aggtcgtgta cccgacctag cagcccaggg atggaaaagt
     5401 cccggccgtc gctggcaata atagcgggcg gacgcatgtc atgagattat tggaaaccac
     5461 cagaatcgaa tataaaaggc gaacaccttt cccaattttg gtttctcctg acccaaagac
     5521 tttaaattta atttatttgt ccctatttca atcaattgaa caactatgga tccaccatgg
     5581 gtgttaagcc agttactttg tatgacgttg ctgaatacgc tggagtttcc taccaaactg
     5641 tctctagagt tgttaatcaa gcttctcatg tctccgctaa gactagagag aaggttgagg
     5701 ctgctatggc tgaattgaac tatattccaa atagagttgc tcagcagttg gctggaaagc
     5761 aatctttgtt gattggagtc gctacttctt ctttggcttt gcatgctcca tctcagattg
     5821 ttgctgctat taagtccaga gctgaccagt tgggagcttc tgttgttgtt tctatggttg
     5881 agagatctgg agttgaggct tgcaaggctg ctgttcataa cttgttggct cagagagttt
     5941 ctggattgat tattaattac ccattggacg atcaagacgc tattgccgtt gaggccgctt
     6001 gtaccaacgt cccagctttg ttcttggacg tttccgatca aactccaatt aattctatta
     6061 ttttttctca cgaggatgga actagattgg gagttgaaca cttggttgct ttgggacatc
     6121 aacagattgc tttgttggct ggaccattgt cttccgtttc tgctagattg agattggccg
     6181 gatggcacaa gtacttgacc agaaaccaga ttcaaccaat tgctgagaga gagggagatt
     6241 ggtctgctat gtctggattc cagcagacta tgcagatgtt gaacgaagga attgtcccaa
     6301 ccgctatgtt ggtcgctaat gaccaaatgg ctttgggagc tatgagagct attactgaat
     6361 ctggattgag agtcggagct gacatttctg ttgttggata tgatgacact gaggattctt
     6421 cttgctacat tccaccattg actactatta agcaagactt cagattgttg ggacagactt
     6481 ctgttgatag attgttgcag ttgtcccaag gacaagctgt taaaggaaac caattgttgc
     6541 cagtttcttt ggttaagaga aagactactt tggctccaaa cactcagact gcttccccaa
     6601 gagctttggc tgactctttg atgcaattgg ctagacaagt ctctagattg gagtctggac
     6661 aaggtggcgg cggctctgtt aacaactcca tgaaggattt cttaggcaag aaaacggtgg
     6721 atggagctga tagtctcaat ttggccgtga atctgcaaca acagcagagt tcaaacacaa
     6781 ttgccaatca atcgctttcc tcaattggat tggaaagttt tggttacggc tctggtatca
     6841 aaaacgagtt taacttccaa gacttgatag gttcaaactc tggcagttca gatccgacat
     6901 tttcagtaga cgctgacgag gcccaaaaac tcgacatttc caacaagaac agtcgtaaga
     6961 gacagaaact aggtttgctg ccggtcagca atgcaacttc ccatttgaac ggtttcaatg
     7021 gaatgtccaa tggaaagtca cactctttct cttcaccgtc tgggactaat gacgatgaac
     7081 taagtggctt gatgttcaac tcaccaagct tcaaccccct cacagttaac gattctacca
     7141 acaacagcaa ccacaatata ggtttgtctc cgatgtcatg cttattttct acagttcaag
     7201 aagcatctca aaaaaagcat ggaaattcca gtagacactt ttcataccca tctgggccgg
     7261 aggacctttg gttcaatgag ttccaaaaac aggccctcac agccaatgga gaaaatgctg
     7321 tccaacaggg agatgatgct tctaagaaca acacagccat tcctaaggac cagtcttcga
     7381 actcatcgat tttcagttca cgttctagtg cagcttctag caactcagga gacgatattg
     7441 gaaggatggg cccattctcc aaaggaccag agattgagtt caactacgat tcttttttgg
     7501 aatcgttgaa ggcagagtca ccctcttctt caaagtacaa tctgccggaa actttgaaag
     7561 agtacatgac ccttagttcg tctcatctga atagtcaaca ctccgacact ttggcaaatg
     7621 gcactaacgg taactattct agcaccgttt ccaacaactt gagcttaagt ttgaactcct
     7681 tctctttctc tgacaagttc tcattgagtc caccaacaat cactgacgcc gaaaagtttt
     7741 cattgatgag aaacttcatt gacaacatct cgccatggtt tgacactttt gacaatacca
     7801 aacagtttgg aacaaaaatt ccagttctgg ccaaaaaatg ttcttcattg tactatgcca
     7861 ttctggctat atcttctcgt caaagagaaa ggataaagaa agagcacaat gaaaaaacat
     7921 tgcaatgcta ccaatactca ctacaacagc tcatccctac tgttcaaagc tcaaataata
     7981 ttgagtacat tatcacatgt attctcctga gtgtgttcca catcatgtct agtgaacctt
     8041 caacccagag ggacatcatt gtgtcattgg caaaatacat tcaagcatgc aacataaacg
     8101 gatttacatc taatgacaaa ctggaaaaga gtattttctg gaactatgtc aatttggatt
     8161 tggctacttg tgcaatcggt gaagagtcaa tggtcattcc ttttagctac tgggttaaag
     8221 agacaactga ctacaagacc attcaagatg tgaagccatt tttcaccaag aagactagca
     8281 cgacaactga cgatgacttg gacgatatgt atgccatcta catgctgtac attagtggta
     8341 gaatcattaa cctgttgaac tgcagagatg cgaagctcaa ttttgagccc aagtgggagt
     8401 ttttgtggaa tgaactcaat gaatgggaat tgaacaaacc cttgaccttt caaagtattg
     8461 ttcagttcaa ggccaatgac gaatcgcagg gcggatcaac ttttccaact gttctattct
     8521 ccaactctcg aagctgttac agtaaccagc tgtatcatat gagctacatc atcttagtgc
     8581 agaataaacc acgattatac aaaatcccct ttactacagt ttctgcttca atgtcatctc
     8641 catcggacaa caaagctggg atgtctgctt ccagcacacc tgcttcagac caccacgctt
     8701 ctggtgatca tttgtctcca agaagtgtag agccctctct ttcgacaacg ttgagccctc
     8761 cgcctaatgc aaacggtgca ggtaacaagt tccgctctac gctctggcat gccaagcaga
     8821 tctgtgggat ttctatcaac aacaaccaca acagcaatct agcagccaaa gtgaactcat
     8881 tgcaaccatt gtggcacgct ggaaagctaa ttagttccaa gtctgaacat acacagttgc
     8941 tgaaactgtt gaacaacctt gagtgtgcaa caggctggcc tatgaactgg aagggcaagg
     9001 agttaattga ctactggaat gttgaagaat aactcgagcc gcggcggccg ccagctttct
     9061 agaacaaaaa ctcatctcag aagaggatct gaatagcgcc gtcgaccatc atcatcatca
     9121 tcattgagtt tgtagcctta gacatgactg ttcctcagtt caagttgggc acttacgaga
     9181 agaccggtct tgctagattc taatcaagag gatgtcagaa tgccatttgc ctgagagatg
     9241 caggcttcat ttttgatact tttttatttg taacctatat agtataggat tttttttgtc
     9301 attttgtttc ttctcgtacg agcttgctcc tgatcagcct atctcgcagc tgatgaatat
     9361 cttgtggtag gggtttggga aaatcattcg agtttgatgt ttttcttggt atttcccact
     9421 cctcttcaga gtacagaaga ttaagtgaga actagtagtt cgtttgtgca agcttatcga
     9481 taagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg caccgtgtat
     9541 gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat
     9601 aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat
     9661 cgccagtcac tatggcgtgc tgctagcgct atatgcgttg atgcaatttc tatgcgcacc
     9721 cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg cttcgctact
     9781 tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga tctatcgaat
     9841 ctaaatgtaa gttaaaatct ctaaataatt aaataagtcc cagtttctcc atacgaacct
     9901 taacagcatt gcggtgagca tctagacctt caacagcagc cagatccatc actgcttggc
     9961 caatatgttt cagtccctca ggagttacgt cttgtgaagt gatgaacttc tggaaggttg
    10021 cagtgttaac tccgctgtat tgacgggcat atccgtacgt tggcaaagtg tggttggtac
    10081 cggaggagta atctccacaa ctctctggag agtaggcacc aacaaacaca gatccagcgt
    10141 gttgtacttg atcaacataa gaagaagcat tctcgatttg caggatcaag tgttcaggag
    10201 cgtactgatt ggacatttcc aaagcctgct cgtaggttgc aaccgatagg gttgtagagt
    10261 gtgcaataca cttgcgtaca atttcaaccc ttggcaactg cacagcttgg ttgtgaacag
    10321 catcttcaat tctggcaagc tccttgtctg tcatatcgac agccaacaga atcacctggg
    10381 aatcaatacc atgttcagct tgagacagaa ggtctgaggc aacgaaatct ggatcagcgt
    10441 atttatcagc aataactaga acttcagaag gcccagcagg catgtcaata ctacacaggg
    10501 ctgatgtgtc attttgaacc atcatcttgg cagcagtaac gaactggttt cctggaccaa
    10561 atattttgtc acacttagga acagtttctg ttccgtaagc catagcagct actgcctggg
    10621 cgcctcctgc tagcacgata cacttagcac caaccttgtg ggcaacgtag atgacttctg
    10681 gggtaagggt accatccttc ttaggtggag atgcaaaaac aatttctttg caaccagcaa
    10741 ctttggcagg aacacccagc atcagggaag tggaaggcag aattgcggtt ccaccaggaa
    10801 tatagaggcc aactttctca ataggtcttg caaaacgaga gcagactaca ccagggcaag
    10861 tctcaacttg caacgtctcc gttagttgag cttcatggaa tttcctgacg ttatctatag
    10921 agagatcaat ggctctctta acgttatctg gcaattgcat aagttcctct gggaaaggag
    10981 cttctaacac aggtgtcttc aaagcgactc catcaaactt ggcagttagt tctaaaaggg
    11041 ctttgtcacc attttgacga acattgtcga caattggttt gactaattcc ataatctgtt
    11101 ccgttttctg gataggacga cgaagggcat cttcaatttc ttgtgaggag gccttagaaa
    11161 cgtcaatttt gcacaattca atacgacctt cagaagggac ttctttaggt ttggattctt
    11221 ctttaggttg ttccttggtg tatcctggct tggcatctcc tttccttcta gtgaccttta
    11281 gggacttcat atccaggttt ctctccacct cgtccaacgt cacaccgtac ttggcacatc
    11341 taactaatgc aaaataaaat aagtcagcac attcccaggc tatatcttcc ttggatttag
    11401 cttctgcaag ttcatcagct tcctccctaa ttttagcgtt caacaaaact tcgtcgtcaa
    11461 ataaccgttt ggtataagaa ccttctggag cattgctctt acgatcccac aaggtggctt
    11521 ccatggctct aagacccttt gattggccaa aacaggaagt gcgttccaag tgacagaaac
    11581 caacacctgt ttgttcaacc acaaatttca agcagtctcc atcacaatcc aattcgatac
    11641 ccagcaactt ttgagttgct ccagatgtag cacctttata ccacaaaccg tgacgacgag
    11701 attggtagac tccagtttgt gtccttatag cctccggaat agactttttg gacgagtaca
    11761 ccaggcccaa cgagtaatta gaagagtcag ccaccaaagt agtgaataga ccatcggggc
    11821 ggtcagtagt caaagacgcc aacaaaattt cactgacagg gaactttttg acatcttcag
    11881 aaagttcgta ttcagtagtc aattgccgag catcaataat ggggattata ccagaagcaa
    11941 cagtggaagt cacatctacc aactttgcgg tctcagaaaa agcataaaca gttctactac
    12001 cgccattagt gaaacttttc aaatcgccca gtggagaaga aaaaggcaca gcgatactag
    12061 cattagcggg caaggatgca actttatcaa ccagggtcct atagataacc ctagcgcctg
    12121 ggatcatcct ttggacaact ctttctgcca aatctaggtc caaaatcact tcattgatac
    12181 cattattgta caacttgagc aagttgtcga tcagctcctc aaattggtcc tctgtaacgg
    12241 atgactcaac ttgcacatta acttgaagct cagtcgattg agtgaacttg atcaggttgt
    12301 gcagctggtc agcagcatag ggaaacacgg cttttcctac caaactcaag gaattatcaa
    12361 actctgcaac acttgcgtat gcaggtagca agggaaatgt catacttgaa gtcggacagt
    12421 gagtgtagtc ttgagaaatt ctgaagccgt atttttatta tcagtgagtc agtcatcagg
    12481 agatcctcta cgccggacgc atcgtggccg acctgcaggg gggggggggc gctgaggtct
    12541 gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca
    12601 gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt
    12661 gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt
    12721 caactcagca aaagttcgat ttattcaaca aagccgccgt cccgtcaagt cagcgtaatg
    12781 ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa
    12841 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
    12901 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
    12961 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
    13021 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
    13081 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
    13141 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
    13201 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
    13261 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
    13321 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
    13381 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
    13441 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
    13501 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
    13561 tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt ttcccgttga
    13621 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
    13681 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
    13741 tccccccccc ccctgcaggt cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
    13801 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
    13861 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
    13921 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
    13981 ctaatgcagg agtcgcataa gggagagcgt cgagtatcta tgattggaag tatgggaatg
    14041 gtgatacccg cattcttcag tgtcttgagg tctcctatca gattatgccc aactaaagca
    14101 accggaggag gagatttcat ggtaaatttc tctgactttt ggtcatcagt agactcgaac
    14161 tgtgagacta tctcggttat gacagcagaa atgtccttct tggagacagt aaatgaagtc
    14221 ccaccaataa agaaatcctt gttatcagga acaaacttct tgtttcgaac tttttcggtg
    14281 ccttgaacta taaaatgtag agtggatatg tcgggtagga atggagcggg caaatgctta
    14341 ccttctggac cttcaagagg tatgtagggt ttgtagatac tgatgccaac ttcagtgaca
    14401 acgttgctat ttcgttcaaa ccattccgaa tccagagaaa tcaaagttgt ttgtctacta
    14461 ttgatccaag ccagtgcggt cttgaaactg acaatagtgt gctcgtgttt tgaggtcatc
    14521 tttgtatgaa taaatctagt ctttgatcta aataatcttg acgagccaga cgataatacc
    14581 aatctaaact ctttaaacgt taaaggacaa gtatgtctgc ctgtattaaa ccccaaatca
    14641 gctcgtagtc tgatcctcat caacttgagg ggcactatct tgttttagag aaatttgcgg
    14701 agatgcgata tcgagaaaaa ggtacgctga ttttaaacgt gaaatttatc tcaagatctg
    14761 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    14821 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    14881 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
    14941 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    15001 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
    15061 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    15121 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    15181 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    15241 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    15301 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    15361 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    15421 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    15481 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    15541 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    15601 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    15661 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    15721 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    15781 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    15841 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    15901 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    15961 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    16021 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    16081 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    16141 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    16201 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    16261 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
    16321 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    16381 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    16441 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    16501 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    16561 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
    16621 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    16681 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    16741 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    16801 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    16861 caat
//
