LOCUS       sequence-246614-       10628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of human GFI1B (HA tagged) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Gomes AM, Kurochkin I, Chang B, Daniel M, Law K, Satija N, Lachmann 
            A, Wang Z, Ferreira L, Ma'ayan A, Chen BK, Papatsenko D, Lemischka 
            IR, Moore KA, Pereira CF
  TITLE     Cooperative Transcription Factor Induction Mediates Hemogenic 
            Reprogramming.
  JOURNAL   Cell Rep. 2018 Dec 4;25(10):2821-2835.e7. doi: 
            10.1016/j.celrep.2018.11.032.
  PUBMED    30517869
REFERENCE   2  (bases 1 to 10628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.11"; journalName: "Cell Rep"; date: 
            "2018-12-4- 4"; volume: "25"; issue: "10"; pages: "2821-2835.e7"
COMMENT     Sequence Label: pLV-tetO-HA-GFI1B
FEATURES             Location/Qualifiers
     source          1..10628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             337..363
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(1384..1958,1959..1961,1962..1970)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1551..1568)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1778..1797
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1971..1973,1974..1976,1977..2690)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1971..1973,1974..1976,1977..2687)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2016..2037)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2277..2296)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2624..2645
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2700..3288
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2753..2773)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3171..3182)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3812..3992
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4018..4035)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4024..4248
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4294..4722
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4381..4400)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4591..4612
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4731..4751)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4736..5065
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4916..5051
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4978..4997
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5113..5160
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5179..5553
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5683..5804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5720..5739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5774..5793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5853..5869)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5853..5869)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5866..5888)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5877..5893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5901..5907,5908..5925,5926..5931))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5946..5967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6084..6101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6255..6843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6335..6354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7014..7805,7806..7874))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7637..7656
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7875..7979)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8054..8073)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8245..8624
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8626..8824
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8842..9022
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9069..9194
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9687..9920
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10105..10149
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10447..10564
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctgact agaattcgcc accatgtacc catacgatgt tccagattac
      361 gcttctagag ctagcatgcc acgctccttc ctggtgaaga gcaagaaggc tcacacctac
      421 caccagcccc gtgtgcagga agatgaaccg ctctggcctc ctgcccttac cccggtgccc
      481 agagaccagg ctccaagcaa cagccctgtc cttagcactc tattcccaaa ccagtgcctg
      541 gactggacca acctcaaacg agagccggag ctggagcagg accagaactt ggccaggatg
      601 gccccggcac cagagggccc cattgtgctg tcccgacccc aggatgggga ctctccactg
      661 tccgactcac ccccattcta caagcctagc ttctcctggg acaccttggc cacaacctat
      721 ggccacagct accggcaggc cccctccacc atgcagtcag ccttcctgga gcactccgtc
      781 agcctgtacg gcagtcctct tgtgcccagc actgagcccg ccttggactt cagcctccgc
      841 tactccccag gcatggatgc gtaccactgt gtgaagtgca acaaggtctt ctccacccct
      901 cacgggctcg aagtgcatgt gcgacgctcc catagtggga cccggccctt cgcctgtgac
      961 atctgcggca aaaccttcgg ccacgctgtg agcctggagc agcacacgca cgtccactcc
     1021 caggagcgca gcttcgagtg ccgcatgtgc ggcaaggcct tcaagcgctc gtccacgctg
     1081 tccacccacc tgctcatcca ctcagacacg cggccctacc cctgccagtt ctgcggcaag
     1141 cgtttccacc agaagtccga catgaagaag cacacctaca tccacacagg tgagaagccg
     1201 cacaagtgcc aggtgtgcgg aaaggccttc agccagagct ccaacctcat cacccacagc
     1261 cgcaagcaca caggcttcaa gcccttcagc tgtgagctgt gcaccaaagg cttccagcgc
     1321 aaggtggacc tgcggcggca ccgcgagagc cagcacaatc tcaagtgaat cgataaggat
     1381 ccgccccwct ccctccccys scsmtaacgt tactggccga agccgcttgg aataaggccg
     1441 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     1501 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     1561 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     1621 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     1681 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     1741 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     1801 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     1861 gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     1921 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca
     1981 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2041 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     2101 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2161 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     2221 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2281 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2341 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2401 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2461 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2521 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2581 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2641 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgca
     2701 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2761 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2821 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2881 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2941 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3001 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3061 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3121 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3181 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3241 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gcctgcaggt
     3301 ttgtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     3361 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     3421 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     3481 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     3541 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     3601 cagatatcca ctgacctttg aatggtgcta caagctagta ccagttgagc aagagaaggt
     3661 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     3721 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     3781 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     3841 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     3901 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     3961 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     4021 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     4081 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     4141 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     4201 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     4261 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     4321 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4381 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4441 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4501 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4561 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4621 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4681 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     4741 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4801 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4861 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4921 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4981 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     5041 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     5101 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     5161 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     5221 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     5281 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     5341 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     5401 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     5461 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     5521 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     5581 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     5641 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     5701 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5761 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     5821 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5881 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5941 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6001 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6061 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6121 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6181 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6661 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6901 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7021 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7081 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7141 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7201 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7261 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7321 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7381 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7441 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7501 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7561 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7621 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7681 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7741 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7801 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7861 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7921 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7981 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     8041 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     8101 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     8161 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     8221 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     8281 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     8341 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     8401 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     8461 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     8521 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     8581 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     8641 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     8701 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     8761 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     8821 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8881 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8941 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9001 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     9061 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     9121 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     9181 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     9241 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     9301 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     9361 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     9421 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     9481 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     9541 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     9601 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     9661 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     9721 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     9781 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     9841 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     9901 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     9961 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    10021 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    10081 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    10141 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    10201 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
    10261 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
    10321 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
    10381 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
    10441 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
    10501 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
    10561 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaacc aaggcagccc
    10621 tggttaac
//
