LOCUS       sequence-246615-        9338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of human GFI1B in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Gomes AM, Kurochkin I, Chang B, Daniel M, Law K, Satija N, Lachmann 
            A, Wang Z, Ferreira L, Ma'ayan A, Chen BK, Papatsenko D, Lemischka 
            IR, Moore KA, Pereira CF
  TITLE     Cooperative Transcription Factor Induction Mediates Hemogenic 
            Reprogramming.
  JOURNAL   Cell Rep. 2018 Dec 4;25(10):2821-2835.e7. doi: 
            10.1016/j.celrep.2018.11.032.
  PUBMED    30517869
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.11"; journalName: "Cell Rep"; date: 
            "2018-12-4- 4"; volume: "25"; issue: "10"; pages: "2821-2835.e7"
COMMENT     Sequence Label: pFUW-tetO-GFI1B
FEATURES             Location/Qualifiers
     source          1..9338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2377..2395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2419..2437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2461..2479
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2503..2521
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2545..2563
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2587..2605
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2638..2676
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2673..2697
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3793..4381
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3846..3866)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4264..4275)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4384..4400)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4385..4401)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4906..5086
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5112..5129)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5118..5342
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5388..5816
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5475..5494)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5685..5706
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5825..5845)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5830..6159
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6010..6145
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6072..6091
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6207..6254
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6273..6647
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6777..6898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6814..6833)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6868..6887
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6947..6963)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6947..6963)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6960..6982)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6971..6987
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6995..7001,7002..7019,7020..7025))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7040..7061
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7178..7195)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7349..7937)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7429..7448)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8108..8899,8900..8968))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8731..8750
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8969..9073)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9148..9167)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttag atctcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2581 taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgaggt
     2641 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2701 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     2761 cgcggccccg aattcatgcc acgctccttc ctggtgaaga gcaagaaggc tcacacctac
     2821 caccagcccc gtgtgcagga agatgaaccg ctctggcctc ctgcccttac cccggtgccc
     2881 agagaccagg ctccaagcaa cagccctgtc cttagcactc tattcccaaa ccagtgcctg
     2941 gactggacca acctcaaacg agagccggag ctggagcagg accagaactt ggccaggatg
     3001 gccccggcac cagagggccc cattgtgctg tcccgacccc aggatgggga ctctccactg
     3061 tccgactcac ccccattcta caagcctagc ttctcctggg acaccttggc cacaacctat
     3121 ggccacagct accggcaggc cccctccacc atgcagtcag ccttcctgga gcactccgtc
     3181 agcctgtacg gcagtcctct tgtgcccagc actgagcccg ccttggactt cagcctccgc
     3241 tactccccag gcatggatgc gtaccactgt gtgaagtgca acaaggtctt ctccacccct
     3301 cacgggctcg aagtgcatgt gcgacgctcc catagtggga cccggccctt cgcctgtgac
     3361 atctgcggca aaaccttcgg ccacgctgtg agcctggagc agcacacgca cgtccactcc
     3421 caggagcgca gcttcgagtg ccgcatgtgc ggcaaggcct tcaagcgctc gtccacgctg
     3481 tccacccacc tgctcatcca ctcagacacg cggccctacc cctgccagtt ctgcggcaag
     3541 cgtttccacc agaagtccga catgaagaag cacacctaca tccacacagg tgagaagccg
     3601 cacaagtgcc aggtgtgcgg aaaggccttc agccagagct ccaacctcat cacccacagc
     3661 cgcaagcaca caggcttcaa gcccttcagc tgtgagctgt gcaccaaagg cttccagcgc
     3721 aaggtggacc tgcggcggca ccgcgagagc cagcacaatc tcaagtgaga attcgatatc
     3781 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3841 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3901 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3961 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4021 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4081 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4141 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     4201 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4261 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4321 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4381 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     4441 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     4501 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     4561 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     4621 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     4681 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     4741 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     4801 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     4861 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     4921 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4981 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     5041 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     5101 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5161 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5221 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5281 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5341 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     5401 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5461 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5521 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5581 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5641 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5701 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5761 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     5821 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     5881 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     5941 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     6001 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6061 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     6121 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     6181 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     6241 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     6301 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     6361 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     6421 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     6481 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     6541 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     6601 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     6661 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6721 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6841 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6901 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6961 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7021 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7081 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7141 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7201 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7261 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7321 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7381 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7441 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7501 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7561 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7621 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7681 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7741 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7801 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7861 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7921 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7981 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8041 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8101 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8161 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8221 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8281 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8341 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8401 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8461 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8521 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8581 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8641 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8701 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8761 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8821 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8881 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8941 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9001 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9061 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     9121 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     9181 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     9241 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     9301 tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
