LOCUS       sequence-246617-        2401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for K.marxianus promoter; type 2 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.P6
FEATURES             Location/Qualifiers
     source          1..2401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(766..800)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(871..1530)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1445..1464
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1712..2300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2201..2220
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acggaattct caaaccgaaa tggggcgttg ttaccccagg tatccggttg
       61 tagttggcac tggggatgga aaaaaatgat gttgatgttg agttagttgg gttgagtcaa
      121 ttagtgcgtg aaagtatcac cacttttgtc atccggcgtt tctgtgcgaa tcacacacac
      181 acacacacac agtttattgg agcgcttgtt tctggcgtat tcgtaattgt tctgcggtgc
      241 ggttctgtgt gcatttttcc tggggtgtct gccgcaccta ctcatcaccc acgccgtggg
      301 tttgagccat ggcggaggta cgactgactg gctgcctgcc tgcctgactg actgcctgac
      361 tgcaggaaaa gagggtttcg aaggaaaaac ttttcctgtg ttaatccggc cgtgcgccgc
      421 tgctccaaaa tccatcttca tgagaaggag tttgaaaaaa caaaaaaatt cacatataaa
      481 aagcgtatct cgagatctca aagtctccct tgaatcgtgt ttgccagttg taactcatcc
      541 tttattcttc tattctatct ctctctttcc ttcccctaat cagcaattaa atccggggta
      601 aggaagaatt actactgtgt gtaacggtta tatttcgttt tttatttttt tttccattgc
      661 catagagaaa gaaaaaaaaa aaaagagagt ttgtgaagat cttccattcg aatcccataa
      721 gtgacacatt taattttttt tttgttagta tgtgagacca gaccaataaa aaacgcccgg
      781 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
      841 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta
      901 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
      961 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     1021 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     1081 ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag
     1141 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     1201 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     1261 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     1321 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     1381 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     1441 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     1501 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     1561 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     1621 tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1681 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1741 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1801 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1861 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1921 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1981 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2041 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2101 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2161 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2221 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2281 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2341 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2401 g
//
