LOCUS       sequence-246622-       13406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13406)
  AUTHORS   Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang
            Y, Cai M
  TITLE     Engineered ethanol-driven biosynthetic system for improving 
            production of acetyl-CoA derived drugs in Crabtree-negative yeast.
  JOURNAL   Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. 
            Epub 2019 May 9.
  PUBMED    31077813
REFERENCE   2  (bases 1 to 13406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-07"; volume: "54:275-284. doi"; pages: " 
            10.1016/j.ymben.2019.05.001. Epub 2019 May 9"
COMMENT     Sequence Label: pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*
FEATURES             Location/Qualifiers
     source          1..13406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        95..1030
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     promoter        1039..1515
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             2591..2620
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2636..2653
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2733..2979
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        2980..3456
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             5626..5655
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5671..5688
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5768..6014
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        6015..6491
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     terminator      8088..8334
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(9159..9974)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9159..9974)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9882..9901
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9966..9985
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(10139..10158)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    10353..11101
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     primer_bind     complement(11136..11158)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11244..11384
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11299..11318
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11399..11416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11570..12158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11650..11669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12329..13120,13121..13189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12952..12971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13190..13294)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13362..13380
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atgtttgaca gcttatcatc gataagctga ctcatgttgg tattgtgaaa tagacgcaga
       61 tcgggaacac tgaaaaataa cagttattat tcgagatcta acatccaaag acgaaaggtt
      121 gaatgaaacc tttttgccat ccgacatcca caggtccatt ctcacacata agtgccaaac
      181 gcaacaggag gggatacact agcagcagac cgttgcaaac gcaggacctc cactcctctt
      241 ctcctcaaca cccacttttg ccatcgaaaa accagcccag ttattgggct tgattggagc
      301 tcgctcattc caattccttc tattaggcta ctaacaccat gactttatta gcctgtctat
      361 cctggccccc ctggcgaggt tcatgtttgt ttatttccga atgcaacaag ctccgcatta
      421 cacccgaaca tcactccaga tgagggcttt ctgagtgtgg ggtcaaatag tttcatgttc
      481 cccaaatggc ccaaaactga cagtttaaac gctgtcttgg aacctaatat gacaaaagcg
      541 tgatctcatc caagatgaac taagtttggt tcgttgaaat gctaacggcc agttggtcaa
      601 aaagaaactt ccaaaagtcg gcataccgtt tgtcttgttt ggtattgatt gacgaatgct
      661 caaaaataat ctcattaatg cttagcgcag tctctctatc gcttctgaac cccggtgcac
      721 ctgtgccgaa acgcaaatgg ggaaacaccc gctttttgga tgattatgca ttgtctccac
      781 attgtatgct tccaagattc tggtgggaat actgctgata gcctaacgtt catgatcaaa
      841 atttaactgt tctaacccct acttgacagc aatatataaa cagaaggaag ctgccctgtc
      901 ttaaaccttt ttttttatca tcattattag cttactttca taattgcgac tggttccaat
      961 tgacaagctt ttgattttaa cgacttttaa cgacaacttg agaagatcaa aaaacaacta
     1021 attattcgaa gactagtctt tttgtagaaa tgtcttggtg tcctcgtcca atcaggtagc
     1081 catctctgaa atatctggct ccgttgcaac tccgaacgac ctgctggcaa cgtaaaattc
     1141 tccggggtaa aacttaaatg tggagtaatg gaaccagaaa cgtctcttcc cttctctctc
     1201 cttccaccgc ccgttaccgt cgctaggaaa ttttactctg ctggagagct tcttctacgg
     1261 cccccttgca gcaatgctct tcccagcatt acgttgcggg taaaacggag gtcgtgtacc
     1321 cgacctagca gcccagggat ggaaaagtcc cggccgtcgc tggcaataat agcgggcgga
     1381 cgcatgtcat gagattattg gaaaccacca gaatcgaata taaaaggcga acacctttcc
     1441 caattttggt ttctcctgac ccaaagactt taaatttaat ttatttgtcc ctatttcaat
     1501 caattgaaca actatatgtc tattccagaa actcaaaaag ccattatctt ctacgaatcc
     1561 aacggcaagt tggagcataa ggatatccca gttccaaagc caaagcccaa cgaattgtta
     1621 atcaacgtca agtactctgg tgtctgccac accgatttgc acgcttggca tggtgactgg
     1681 ccattgccaa ctaagttacc attagttggt ggtcacgaag gtgccggtgt cgttgtcggc
     1741 atgggtgaaa acgttaaggg ctggaagatc ggtgactacg ccggtatcaa atggttgaac
     1801 ggttcttgta tggcctgtga atactgtgaa ttgggtaacg aatccaactg tcctcacgct
     1861 gacttgtctg gttacaccca cgacggttct ttccaagaat acgctaccgc tgacgctgtt
     1921 caagccgctc acattcctca aggtactgac ttggctgaag tcgcgccaat cttgtgtgct
     1981 ggtatcaccg tatacaaggc tttgaagtct gccaacttga gagcaggcca ctgggcggcc
     2041 atttctggtg ctgctggtgg tctaggttct ttggctgttc aatatgctaa ggcgatgggt
     2101 tacagagtct taggtattga tggtggtcca ggaaaggaag aattgtttac ctcgctcggt
     2161 ggtgaagtat tcatcgactt caccaaagag aaggacattg ttagcgcagt cgttaaggct
     2221 accaacggcg gtgcccacgg tatcatcaat gtttccgttt ccgaagccgc tatcgaagct
     2281 tctaccagat actgtagggc gaacggtact gttgtcttgg ttggtttgcc agccggtgca
     2341 aagtgctcct ctgatgtctt caaccacgtt gtcaagtcta tctccattgt cggctcttac
     2401 gtggggaaca gagctgatac cagagaagcc ttagatttct ttgccagagg tctagtcaag
     2461 tctccaataa aggtagttgg cttatccagt ttaccagaaa tttacgaaaa gatggagaag
     2521 ggccaaattg ctggtagata cgttgttgac acttctaaat aatcgagccg cggcggccgc
     2581 cagctttcta gaacaaaaac tcatctcaga agaggatctg aatagcgccg tcgaccatca
     2641 tcatcatcat cattgagttt gtagccttag acatgactgt tcctcagttc aagttgggca
     2701 cttacgagaa gaccggtctt gctagattct aatcaagagg atgtcagaat gccatttgcc
     2761 tgagagatgc aggcttcatt tttgatactt ttttatttgt aacctatata gtataggatt
     2821 ttttttgtca ttttgtttct tctcgtacga gcttgctcct gatcagccta tctcgcagct
     2881 gatgaatatc ttgtggtagg ggtttgggaa aatcattcga gtttgatgtt tttcttggta
     2941 tttcccactc ctcttcagag tacagaagat taagtgagat ttttgtagaa atgtcttggt
     3001 gtcctcgtcc aatcaggtag ccatctctga aatatctggc tccgttgcaa ctccgaacga
     3061 cctgctggca acgtaaaatt ctccggggta aaacttaaat gtggagtaat ggaaccagaa
     3121 acgtctcttc ccttctctct ccttccaccg cccgttaccg tccctaggaa attttactct
     3181 gctggagagc ttcttctacg gcccccttgc agcaatgctc ttcccagcat tacgttgcgg
     3241 gtaaaacgga ggtcgtgtac ccgacctagc agcccaggga tggaaaagtc ccggccgtcg
     3301 ctggcaataa tagcgggcgg acgcatgtca tgagattatt ggaaaccacc agaatcgaat
     3361 ataaaaggcg aacacctttc ccaattttgg tttctcctga cccaaagact ttaaatttaa
     3421 tttatttgtc cctatttcaa tcaattgaac aactatatgt cgccctctgc cgtacaatca
     3481 tcaaaactag aagaacagtc aagtgaaatt gacaagttga aagcaaaaat gtcccagtct
     3541 gcctccactg cgcagcagaa gaaggaacat gagtatgaac atttgacctc ggtcaagatc
     3601 gtgccacaac ggcccatctc agatagactg cagcccgcaa ttgctaccca ctattctcca
     3661 cacttggacg ggttgcagga ctatcagcgc ttgcacaagg agtctattga agaccctgct
     3721 aagttcttcg gttctaaagc tacccaattt ttaaactggt ctaagccatt cgataaggtg
     3781 ttcatcccag actctaaaac gggtaggccc tccttccaga acaatgcatg gttcctcaac
     3841 ggccaattaa acgcctgtta caactgtgtt gacagacatg ccttgaagac ccctaacaag
     3901 aaagccatta ttttcgaagg tgacgagcct ggccaaggct attccattac ctacaaggaa
     3961 ctacttgaag aagtttgtca agtggcacaa gtgctgactt actctatggg cgttcgcaag
     4021 ggcgatactg ttgccgtgta catgcctatg gtcccagaag caatcataac cttgttggcc
     4081 atttcccgta tcggcgccat tcactccgta gtctttgccg ggttttcttc caactccttg
     4141 agagatcgta tcaacgatgg ggactctaaa gttgtcatca ctacagatga atccaacaga
     4201 ggtggtaaag tcattgagac taaaagaatt gttgatgacg cgctaagaga gaccccaggc
     4261 gtgagacacg tcttggttta tagaaagacc aacaatccat ctgttgcttt ccatgccccc
     4321 agagatttag attgggcaac agaaaagaag aaatacaaga cctactatcc atgcacaccc
     4381 gttgattctg aggatccatt attcttgttg tatacgtctg gttctactgg tgcccccaag
     4441 ggtgttcaac attctaccgc aggttacttg ctgggagctt tgttgaccat gcgctacact
     4501 tttgacactc accaagaaga cgttttcttc acagctggag acattggctg gattacaggc
     4561 cacacttatg tggtttatgg tcccttacta tatggttgtg ccactttggt ctttgaaggg
     4621 actcctgcgt acccaaatta ctcccgttat tgggatatta ttgatgaaca caaagtcacc
     4681 caattttatg ttgccccaac tgctttgcgt ttgttgaaaa gagctggtga ttcctacatc
     4741 gaaaatcatt ccttaaaatc tttgcgttgc ttgggttcgg tcggtgaacc aattgctgct
     4801 gaagtttggg agtggtactc tgaaaaaata ggtaaaaatg aaatccccat tgtagacacc
     4861 tactggcaaa cagaatctgg ttcgcatctg gtcaccccgc tggctggtgg tgtcacacca
     4921 atgaaaccgg gttctgcctc attccccttc ttcggtattg atgcagttgt tcttgaccct
     4981 aacactggtg aagaacttaa taccagccac gcagagggtg tccttgccgt caaagctgca
     5041 tggccatcat ttgcaagaac tatttggaaa aatcatgata ggtatctaga cacttatttg
     5101 aacccttacc ctggctacta tttcactggt gatggtgctg caaaggataa ggatggttat
     5161 atctggattt tgggtcgtgt agacgatgtg gtgaacgtct ctggtcaccg tctgtctacc
     5221 gctgaaattg aggctgctat tatcgaagat ccaattgtgg ccgagtgtgc tgttgtcgga
     5281 ttcaacgatg acttgactgg tcaagcagtt gctgcatttg tggtgttgaa aaacaaatct
     5341 aattggtcca ccgcaacaga tgatgaatta caagatatca agaagcattt ggtctttact
     5401 gttagaaaag acatcgggcc atttgccgca ccaaaattga tcattttagt ggatgacttg
     5461 cccaagacaa gatctggcaa aattatgaga cgtattttaa gaaaaatcct agcaggagaa
     5521 agtgaccaac taggcgacgt ttctacattg tcaaaccctg gcattgttag acatccaatt
     5581 gattcggtca agttgcctcg agccgcggcg gccgccagct ttctagaaca aaaactcatc
     5641 tcagaagagg atctgaatag cgccgtcgac catcatcatc atcatcattg agtttgtagc
     5701 cttagacatg actgttcctc agttcaagtt gggcacttac gagaagaccg gtcttgctag
     5761 attctaatca agaggatgtc agaatgccat ttgcctgaga gatgcaggct tcatttttga
     5821 tactttttta tttgtaacct atatagtata ggattttttt tgtcattttg tttcttctcg
     5881 tacgagcttg ctcctgatca gcctatctcg cagctgatga atatcttgtg gtaggggttt
     5941 gggaaaatca ttcgagtttg atgtttttct tggtatttcc cactcctctt cagagtacag
     6001 aagattaagt gagatttttg tagaaatgtc ttggtgtcct cgtccaatca ggtagccatc
     6061 tctgaaatat ctggctccgt tgcaactccg aacgacctgc tggcaacgta aaattctccg
     6121 gggtaaaact taaatgtgga gtaatggaac cagaaacgtc tcttcccttc tctctccttc
     6181 caccgcccgt taccgtccct aggaaatttt actctgctgg agagcttctt ctacggcccc
     6241 cttgcagcaa tgctcttccc agcattacgt tgcgggtaaa acggaggtcg tgtacccgac
     6301 ctagcagccc agggatggaa aagtcccggc cgtcgctggc aataatagcg ggcggacgca
     6361 tgtcatgaga ttattggaaa ccaccagaat cgaatataaa aggcgaacac ctttcccaat
     6421 tttggtttct cctgacccaa agactttaaa tttaatttat ttgtccctat ttcaatcaat
     6481 tgaacaacta tatgactaag ctacactttg acactgctga accagtcaag atcacacttc
     6541 caaatggttt gacatacgag caaccaaccg gtctattcat taacaacaag tttatgaaag
     6601 ctcaagacgg taagacctat cccgtcgaag atccttccac tgaaaacacc gtttgtgagg
     6661 tctcttctgc caccactgaa gatgttgaat atgctatcga atgtgccgac cgtgctttcc
     6721 acgacactga atgggctacc caagacccaa gagaaagagg ccgtctacta agtaagttgg
     6781 ctgacgaatt ggaaagccaa attgacttgg tttcttccat tgaagctttg gacaatggta
     6841 aaactttggc cttagcccgt ggggatgtta ccattgcaat caactgtcta agagatgctg
     6901 ctgcctatgc cgacaaagtc aacggtagaa caatcaacac cggtgacggc tacatgaact
     6961 tcaccacctt agagccaatc ggtgtctgtg gtcaaattat tccatggaac tttccaataa
     7021 tgatgttggc ttggaagatc gccccagcat tggccatggg taacgtctgt atcttgaaac
     7081 ccgctgctgt cacaccttta aatgccctat actttgcttc tttatgtaag aaggttggta
     7141 ttccagctgg tgtcgtcaac atcgttccag gtcctggtag aactgttggt gctgctttga
     7201 ccaacgaccc aagaatcaga aagctggctt ttaccggttc tacagaagtc ggtaagagtg
     7261 ttgctgtcga ctcttctgaa tctaacttga agaaaatcac tttggaacta ggtggtaagt
     7321 ccgcccattt ggtctttgac gatgctaaca ttaagaagac tttaccaaat ctagtaaacg
     7381 gtattttcaa gaacgctggt caaatttgtt cctctggttc tagaatttac gttcaagaag
     7441 gtatttacga cgaactattg gctgctttca aggcttactt ggaaaccgaa atcaaagttg
     7501 gtaatccatt tgacaaggct aacttccaag gtgctatcac taaccgtcaa caattcgaca
     7561 caattatgaa ctacatcgat atcggtaaga aagaaggcgc caagatctta actggtggcg
     7621 aaaaagttgg tgacaagggt tacttcatca gaccaaccgt tttctacgat gttaatgaag
     7681 acatgagaat tgttaaggaa gaaatttttg gaccagttgt cactgtcgca aagttcaaga
     7741 ctttagaaga aggtgtcgaa atggctaaca gctctgaatt cggtctaggt tctggtatcg
     7801 aaacagaatc tttgagcaca ggtttgaagg tggccaagat gttgaaggcc ggtaccgtct
     7861 ggatcaacac atacaacgat tttgactcca gagttccatt cggtggtgtt aagcaatctg
     7921 gttacggtag agaaatgggt gaagaagtct accatgcata cactgaagta aaagctgtca
     7981 gaattaagtt gtaacctagg gcggccgcga attaattcgc cttagacatg actgttcctc
     8041 agttcaagtt gggcacttac gagaagaccg gtcttgctag attctaatca agaggatgtc
     8101 agaatgccat ttgcctgaga gatgcaggct tcatttttga tactttttta tttgtaacct
     8161 atatagtata ggattttttt tgtcattttg tttcttctcg tacgagcttg ctcctgatca
     8221 gcctatctcg cagctgatga atatcttgtg gtaggggttt gggaaaatca ttcgagtttg
     8281 atgtttttct tggtatttcc cactcctctt cagagtacag aagattaagt gagacgttcg
     8341 tttgtgcaag cttatcgata agctttaatg cggtagttta tcacagttaa attgctaacg
     8401 cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac
     8461 cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat
     8521 cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat
     8581 gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt
     8641 cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt
     8701 cctgtggatc tatcgaatct aaatgtaagt taaaatctct aaataaactt gaagtcggac
     8761 agtgagtgta gtcttgagaa attctgaagc cgtattttta ttatcagtga gtcagtcatc
     8821 aggagatcct ctacgccgga cgcatcgtgg ccgacctgca gggggggggg ggcgctgagg
     8881 tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc catcatccag
     8941 ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat
     9001 tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc
     9061 cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca agtcagcgta
     9121 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
     9181 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     9241 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     9301 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     9361 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     9421 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     9481 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     9541 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     9601 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     9661 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     9721 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     9781 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     9841 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     9901 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt
     9961 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
    10021 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
    10081 ctttcccccc cccccctgca ggtcggcatc accggcgcca caggtgcggt tgctggcgcc
    10141 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
    10201 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
    10261 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
    10321 ttcctaatgc aggagtcgca taagggagag cgtcgagtat ctatgattgg aagtatggga
    10381 atggtgatac ccgcattctt cagtgtcttg aggtctccta tcagattatg cccaactaaa
    10441 gcaaccggag gaggagattt catggtaaat ttctctgact tttggtcatc agtagactcg
    10501 aactgtgaga ctatctcggt tatgacagca gaaatgtcct tcttggagac agtaaatgaa
    10561 gtcccaccaa taaagaaatc cttgttatca ggaacaaact tcttgtttcg aactttttcg
    10621 gtgccttgaa ctataaaatg tagagtggat atgtcgggta ggaatggagc gggcaaatgc
    10681 ttaccttctg gaccttcaag aggtatgtag ggtttgtaga tactgatgcc aacttcagtg
    10741 acaacgttgc tatttcgttc aaaccattcc gaatccagag aaatcaaagt tgtttgtcta
    10801 ctattgatcc aagccagtgc ggtcttgaaa ctgacaatag tgtgctcgtg ttttgaggtc
    10861 atctttgtat gaataaatct agtctttgat ctaaataatc ttgacgagcc agacgataat
    10921 accaatctaa actctttaaa cgttaaagga caagtatgtc tgcctgtatt aaaccccaaa
    10981 tcagctcgta gtctgatcct catcaacttg aggggcacta tcttgtttta gagaaatttg
    11041 cggagatgcg atatcgagaa aaaggtacgc tgattttaaa cgtgaaattt atctcatgat
    11101 ctctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
    11161 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    11221 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
    11281 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    11341 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
    11401 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    11461 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    11521 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    11581 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    11641 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    11701 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    11761 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    11821 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    11881 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    11941 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    12001 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    12061 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    12121 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    12181 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    12241 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    12301 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    12361 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    12421 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    12481 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    12541 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    12601 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
    12661 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    12721 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    12781 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    12841 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    12901 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
    12961 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    13021 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    13081 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    13141 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    13201 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    13261 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    13321 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    13381 cctttcgtct tcaagaatta attctc
//
