LOCUS       sequence-246623-        2841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Storage plasmid for selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pKmK.M1
FEATURES             Location/Qualifiers
     source          1..2841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..313
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             314..988
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     terminator      complement(1206..1240)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1311..1970)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1885..1904
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2152..2740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2641..2660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat acacaatcag taaaaatcaa cggttaacga cattactata tatataatat
       61 aggaagcatt taatagaaca gcatcgtaat atatgtgtac tttgcagtta tgacgccaga
      121 tggcagtagt ggaagatatt ctttattgaa aaatagcttg tcaccttacg tacaatcttg
      181 atccggagct tttctttttt tgccgattaa gaattcggtc gaaaaaagaa aaggagaggg
      241 ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta agcacacaaa
      301 ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat tggtgaaagt
      361 ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg ctgacttgct
      421 gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg caaggaaaat
      481 ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact tggttggcgt
      541 gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg gcattgatat
      601 cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg gtttgccagt
      661 tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag cttcacagaa
      721 acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg aacttttgga
      781 ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct tacattttat
      841 gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa atggcgttat
      901 tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta acaaaatagc
      961 aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat ttaagtattg
     1021 tttgtgcact tgcctgcagg ccttttgaaa agcaagcata aaagatctaa acataaaatc
     1081 tgtaaaataa caagatgtaa agataatgct aaatcatttg gctttttgat tgattgtaca
     1141 ggaaaatata catcgcaggg ggttgacttt taccatttca ccgcaatgga gttgagacca
     1201 gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     1261 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
     1321 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     1381 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     1441 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     1501 tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac
     1561 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     1621 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     1681 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     1741 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     1801 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     1861 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     1921 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     1981 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     2041 tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga
     2101 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2161 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2221 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2281 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2341 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2401 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2461 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2521 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2581 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2641 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2701 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2761 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2821 tgattctgtg gataaccgta g
//
