LOCUS       sequence-246624-        9620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminal Flag-tagged yeast Trl1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Unlu I, Lu Y, Wang X
  TITLE     The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune 
            noncanonical XBP1 splicing during ER stress.
  JOURNAL   J Biol Chem. 2018 Dec 14;293(50):19365-19376. doi: 
            10.1074/jbc.RA118.004872. Epub 2018 Oct 24.
  PUBMED    30355738
REFERENCE   2  (bases 1 to 9620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.004872"; journalName: "J Biol Chem"; date: 
            "2018-12-14- 14"; volume: "293"; issue: "50"; pages: "19365-19376"
COMMENT     Sequence Label: pB-bsd-Flag-Trl1
FEATURES             Location/Qualifiers
     source          1..9620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          112..1128
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1052..1074
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1136..1155
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     polyA_signal    3795..4019
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4392..4454
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4520..4539)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4521..4539)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4546..4563)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4546..4562)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4555..4577)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4704..5159
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4791..4810)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5001..5022
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5185..5289
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5290..5358,5359..6150)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5508..5527)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6321..6909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6810..6829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7063..7080
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7197..7218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7233..7238,7239..7256,7257..7263)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7271..7287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7276..7298
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7295..7311
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7295..7311
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7332..7350
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   7499..7533
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    7934..8158
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(8186..8584)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MGMPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      8581..8590
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8604..9103)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8652..8671)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9045..9068
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     enhancer        9123..9502
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggsggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
       61 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct
      121 gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
      181 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
      241 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
      301 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
      361 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
      421 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
      481 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
      541 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
      601 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
      661 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
      721 cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga
      781 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt
      841 cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc
      901 gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
      961 gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac
     1021 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     1081 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     1141 gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tggatcagct tggcaaagca
     1201 ttcatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
     1261 gacgatgacc ggatccgaat tctgatgcct agcccatatg acggtaaaag aacagtaact
     1321 cagcttgtta acgagttaga aaaggcagaa aaactatccg gtagaggcag agcttataga
     1381 agggtttgtg acttatcaca cagcaataag aaagtaattt catggaaatt taacgaatgg
     1441 gattatggca aaaatactat cactttgcca tgtaatgcaa gaggcttatt tattagcgat
     1501 gatacaacga atccagttat tgttgctaga ggatacgata agtttttcaa tgtaggcgag
     1561 gtcaacttta ccaagtggaa ttggattgaa gagaactgta cagggcctta cgatgtcact
     1621 ataaaagcca atggttgtat catttttata tctggtttag aagatggtac cttggtagtt
     1681 tgttcgaagc attctactgg acccagagca gacgtagaca ggaaccatgc agaagcaggt
     1741 gagaagcaac ttttaagaca actggcggca atgaacatca atcgaagtga ttttgctaga
     1801 atgctatata cccataatgt caccgctgtg gcagaatatt gcgatgattc atttgaagaa
     1861 cacatcttag agtatcccct tgaaaaagct ggcctatact tacatggtgt aaatgttaat
     1921 aaagcggaat ttgaaacttg ggatatgaaa gatgtttcgc aaatggcgag taaatacgga
     1981 ttcaggtgcg tccaatgcat tacatcgaac accttggagg atttgaaaaa gttcctagat
     2041 aactgctctg caaccggatc ttttgaaggg caagagattg aaggttttgt tatcaggtgc
     2101 cacttgaaga gtactgaaaa gccatttttt ttcaagtata aatttgaaga accgtatttg
     2161 atgtaccgtc agtggagaga agttactaaa gactatattt ccaacaagtc aagagtgttt
     2221 aaatttagaa agcataaatt tataaccaac aagtatcttg atttcgcaat cccaatattg
     2281 gagtcatcac ccaagatctg tgagaattac ttgaaaggtt ttggtgtgat tgaattgaga
     2341 aataagtttc tacaatcata tggcatgagt gggctggaaa tcttaaacca cgaaaaagtg
     2401 gccgagttgg aattaaaaaa cgccattgat tatgataaag tagacgaacg cactaaattt
     2461 ttaatctttc caatatcagt tattggatgt ggcaaaacaa caacttccca gacattagtg
     2521 aatttgttcc ctgacagctg gggtcatatt caaaatgatg atattacagg taaagataaa
     2581 tctcaattaa tgaaaaaatc attggaacta ttatccaaaa aagaaatcaa atgtgtcata
     2641 gttgacagaa acaatcacca attccgcgaa agaaagcaat tatttgaatg gttgaatgaa
     2701 ctaaaagaag attatttggt gtatgatacg aacatcaagg taatcggcgt gtcattcgcg
     2761 ccatatgaca aactatcgga aataagagac ataacactgc aaagagtaat caaaagaggt
     2821 aacaatcacc aaagtattaa atgggacgaa ttaggagaga agaaagtggt aggcatcatg
     2881 aatgggtttt tgaaaagata tcaacctgtt aatttagaca aatcaccaga taatatgttt
     2941 gatttaatga tagagttaga ttttggacaa gcagactctt cattaaccaa tgcgaaacaa
     3001 atcctcaatg aaattcataa agcttatcca attttggtac ctgagatccc gaaagatgat
     3061 gaaattgaga cagcgttcag aaggagctta gattacaaac ctactgtgag aaaaatagtt
     3121 ggtaaaggca acaataatca acaaaaaacg cctaagttaa ttaaacctac atatatctca
     3181 gctaaaatag aaaactacga tgaaattatt gaattggtca agaggtgtat tgctagcgat
     3241 gcagagctga ctgagaaatt caagcattta ctagctagtg gaaaagttca gaaggaactt
     3301 catataacat taggtcatgt catgtcgtcc cgtgaaaaag aggcaaaaaa gttgtggaaa
     3361 tcatattgta acagatacac tgaccagata acggagtaca ataacaaccg catagaaaat
     3421 gcacaaggct ctggtaataa ccaaaatacg caggtaaaaa cgaccgacaa actgaatttt
     3481 aggctcgaaa aactatgttg ggatgaaaaa atcattgcta ttgtagtgga actgtctaaa
     3541 gataaagatg gatgcataat tgatgaaaac aatgagaaaa ttaaagggtt gtgttgtcaa
     3601 aacaaaattc ctcatattac gctgtgcaaa cttgaaagtg gtgttaaggc tgtttattcc
     3661 aatgttctat gtgagaaggt tgaatctgct gaagttgatg aaaatataaa agtggtgaaa
     3721 ttggacaact cgaaggaatt tgttggcagt gtatatttaa atttttagac tcgagggccg
     3781 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     3841 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     3901 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     3961 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     4021 ttctgaggcg gaaagaacca gctggggctt aattaacgag agcataatat tgatatgtgc
     4081 caaagttgtt tctgactgac taataagtat aatttgtttc tattatgtat aggttaagct
     4141 aattacttat tttataatac aacatgactg tttttaaagt acaaaataag tttatttttg
     4201 taaaagagag aatgtttaaa agttttgtta ctttatagaa gaaattttga gtttttgttt
     4261 ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat tagtatgtaa
     4321 gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc gatatacaga
     4381 ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt cacaatatga
     4441 ttatctttct agggttaaat aatagtttct aattttttta ttattcagcc tgctgtcgtg
     4501 aataccgagc tccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
     4561 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     4621 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     4681 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4741 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4801 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4861 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4921 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4981 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5041 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5101 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     5161 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5221 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5281 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5341 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5401 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5461 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5521 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5581 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5641 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5701 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5761 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5821 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5881 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5941 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6001 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6061 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6121 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6181 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6241 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6301 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6361 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6421 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6481 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6541 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6601 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6661 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6721 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6781 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6841 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6901 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6961 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7021 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7081 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7141 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7201 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7261 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7321 gccaagctcg aaattaaccc tcactaaagg gaacaaaagc tggtacctcg cgcgacttgg
     7381 tttgccattc tttagcgcgc gtcgcgtcac acagcttggc cacaatgtgg tttttgtcaa
     7441 acgaagattc tatgacgtgt ttaaagttta ggtcgagtaa agcgcaaatc ttttttaacc
     7501 ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc tctctctttc
     7561 taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct tggagctccc
     7621 gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac gaccgcgtga
     7681 gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt
     7741 atattgttat ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat
     7801 agatatcgtg actaatatat aataaaatgg gtagttcttt agacgatgag catatcctct
     7861 ctgctcttct gcaaagcgat gacgagcttg ttggctagcg tcgagcccca gctggttctt
     7921 tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
     7981 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
     8041 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
     8101 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc
     8161 gaggctgatc agcgagctct agagcttagc cctcccacac ataaccagag ggcagcaatt
     8221 cacgaatccc aactgccgtc ggctgtccat cactgtcctt cactatggct ttgatcccag
     8281 gatgcagatc gagaagcacc tgtcgacacc gtccgcaggg gctcaagatg cccctgttct
     8341 catttccgat cgcgacgata caagtcaggt tgccagctgc cgcagcagca gcagtgccca
     8401 gcaccacgag ttctgcacaa ggtcccccag taaaatgata tacattgaca ccagtgaaga
     8461 tgcggccgtc gctagagaga gctgcgctgg cgacgctgta gtcttcagag atggggatgc
     8521 tgttgattgt agccgttgct ctttcaatga gggtggattc ttcttgagac aaaggcatcc
     8581 ccatggtggc gttggctgca ggtcgaaagg cccggagatg aggaagagga gaacagcgcg
     8641 gcagacgtgc gcttttgaag cgtgcagaat gccgggcctc cggaggacct tcgggcgccc
     8701 gccccgcccc tgagcccgcc cctgagcccg cccccggacc caccccttcc cagcctctga
     8761 gcccagaaag cgaaggagca aagctgctat tggccgctgc cccaaaggcc tacccgcttc
     8821 cattgctcag cggtgctgtc catctgcacg agactagtga gacgtgctac ttccatttgt
     8881 cacgtcctgc acgacgcgag ctgcggggcg ggggggaact tcctgactag gggaggagta
     8941 gaaggtggcg cgaaggggcc accaaagaac ggagccggtt ggcgcctacc ggtggatgtg
     9001 gaatgtgtgc gaggccagag gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa
     9061 gcgcatgctc cagactgcct tgggaaaagc gcctccccta cccggtagaa ttaattcgat
     9121 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     9181 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     9241 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     9301 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     9361 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     9421 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     9481 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     9541 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     9601 gatgggggcg gggggggggg
//
