LOCUS       sequence-246627-       16806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast plasmid overexpressing AtX and NpgA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16806)
  AUTHORS   Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang
            Y, Cai M
  TITLE     Engineered ethanol-driven biosynthetic system for improving 
            production of acetyl-CoA derived drugs in Crabtree-negative yeast.
  JOURNAL   Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. 
            Epub 2019 May 9.
  PUBMED    31077813
REFERENCE   2  (bases 1 to 16806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-07"; volume: "54:275-284. doi"; pages: " 
            10.1016/j.ymben.2019.05.001. Epub 2019 May 9"
COMMENT     Sequence Label: pK_-P-GAP-NpgA+AtX
FEATURES             Location/Qualifiers
     source          1..16806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        119..1054
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     promoter        1063..1539
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             6988..7005
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7085..7331
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        7332..7808
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     RBS             8389..8397
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8862..8879
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      8976..9222
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(9635..12169)
                     /codon_start=1
                     /gene="Pichia pastoris HIS4"
                     /product="multifunctional enzyme, required for histidine 
                     biosynthesis"
                     /label=PpHIS4
                     /note="auxotrophic marker for Pichia pastoris"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRHDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     CDS             complement(12582..13397)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(12582..13397)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     13305..13324
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     13389..13408
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(13562..13581)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    13776..14524
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     primer_bind     complement(14560..14582)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14668..14808
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14723..14742
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14823..14840)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14994..15582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15074..15093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15753..16544,16545..16613))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16376..16395
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16614..16718)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     16786..16804
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttcgtcttc aagaattaat tctcatgttt gacagcttat catcgataag ctgactcatg
       61 ttggtattgt gaaatagacg cagatcggga acactgaaaa ataacagtta ttattcgaga
      121 tctaacatcc aaagacgaaa ggttgaatga aacctttttg ccatccgaca tccacaggtc
      181 cattctcaca cataagtgcc aaacgcaaca ggaggggata cactagcagc agaccgttgc
      241 aaacgcagga cctccactcc tcttctcctc aacacccact tttgccatcg aaaaaccagc
      301 ccagttattg ggcttgattg gagctcgctc attccaattc cttctattag gctactaaca
      361 ccatgacttt attagcctgt ctatcctggc ccccctggcg aggttcatgt ttgtttattt
      421 ccgaatgcaa caagctccgc attacacccg aacatcactc cagatgaggg ctttctgagt
      481 gtggggtcaa atagtttcat gttccccaaa tggcccaaaa ctgacagttt aaacgctgtc
      541 ttggaaccta atatgacaaa agcgtgatct catccaagat gaactaagtt tggttcgttg
      601 aaatgctaac ggccagttgg tcaaaaagaa acttccaaaa gtcggcatac cgtttgtctt
      661 gtttggtatt gattgacgaa tgctcaaaaa taatctcatt aatgcttagc gcagtctctc
      721 tatcgcttct gaaccccggt gcacctgtgc cgaaacgcaa atggggaaac acccgctttt
      781 tggatgatta tgcattgtct ccacattgta tgcttccaag attctggtgg gaatactgct
      841 gatagcctaa cgttcatgat caaaatttaa ctgttctaac ccctacttga cagcaatata
      901 taaacagaag gaagctgccc tgtcttaaac cttttttttt atcatcatta ttagcttact
      961 ttcataattg cgactggttc caattgacaa gcttttgatt ttaacgactt ttaacgacaa
     1021 cttgagaaga tcaaaaaaca actaattatt cgaagactag tctttttgta gaaatgtctt
     1081 ggtgtcctcg tccaatcagg tagccatctc tgaaatatct ggctccgttg caactccgaa
     1141 cgacctgctg gcaacgtaaa attctccggg gtaaaactta aatgtggagt aatggaacca
     1201 gaaacgtctc ttcccttctc tctccttcca ccgcccgtta ccgtccctag gaaattttac
     1261 tctgctggag agcttcttct acggccccct tgcagcaatg ctcttcccag cattacgttg
     1321 cgggtaaaac ggaggtcgtg tacccgacct agcagcccag ggatggaaaa gtcccggccg
     1381 tcgctggcaa taatagcggg cggacgcatg tcatgagatt attggaaacc accagaatcg
     1441 aatataaaag gcgaacacct ttcccaattt tggtttctcc tgacccaaag actttaaatt
     1501 taatttattt gtccctattt caatcaattg aacaactatt tcgaaacgag gaccatgggt
     1561 atggaggtac atggagatga agtgttgtca gtcgactctg gcatctcaac tccgccctcg
     1621 acaggaggtg gatttcggag gccactagag actcccggaa cagaaatcgg gaatctcaat
     1681 cttaaccctc agaatgaggt tgccgttgtt ggaatggcct gccggcttgc cgggggcaat
     1741 cattctccag aagaactgtg gcagtccatt ctaaacagga aggatgcctc tggcgagatc
     1801 ccaagcatgc gctgggagcc gtactaccgt cgggacattc gcaaccccaa gatcctagat
     1861 caaacgacaa agcgcggcta cttcttggac cacatcgaga attttgatgc cgcgttcttt
     1921 ggcgtttccc ccaaagaggc cgagcagatg gacccccagc agcggttgtc actcgaggtg
     1981 acttgggagg ccctggaaga cgcaggaatc ccaccgcaga gtttgtccgg ctcagaaaca
     2041 gccgtgttta tgggggtcaa ttcggatgat tattccaagc tcttactgga agatattccg
     2101 aacgtggagg cctggatggg catcggcact gcgtactgcg gagtcccgaa ccgcatctcc
     2161 taccacctga acctcatggg gcccagcact gccgttgatg ccgcctgtgc ctcctctctc
     2221 gttgccatcc atcacggacg acaggccatc ctacaaggcg agagcgaagt cgctattgtc
     2281 ggaggagtca acgctctctg cgggccagga ctgactcgcg tactcgataa ggcaggagcg
     2341 acctccacgg aaggtcgctg tctctctttc gacgaagatg cgaagggcta cggccgtggt
     2401 gaaggagctg cggtggtgat cttgaaacgg ctgtccaccg ccatccggga cggagaccac
     2461 attcgcgcca tcatcaaggg tagtgccgta gcacaggatg gcaaaaccaa cggcatcatg
     2521 gctcccaacg ccaaggcaca agagcttgtg gcatggaatg ctcttcggac agccggagtc
     2581 gaccctctga cggttgggta tgtggaagct cacgcaacgt caacccctct tggcgatcct
     2641 accgaggtca gcgccgtctc agcagtctac ggcaaaggca gaccggaagg gaatccttgc
     2701 ttcattggct ctgtcaaacc caacgtgggc catttggaag cgggcgctgg cgccgtcggt
     2761 ttcatcaaag cagtcatggc agttgaaaag gccactttcc ccccacaaac caacctgaag
     2821 agactcaatt ctcgcattga ctgggaccaa gccggagtga aggtcgtcca ggagacactg
     2881 gaatggcctg gcaatgagga tgacgtccgc cgagccggtg tttgctctta cggatatggc
     2941 ggtacggtct cccatgcaat catcgaggag tttgcgcaac agctccaacg gccgactacc
     3001 aacacaaccg atgaagagcc tctgcctcgg attcttctgc tgtcggcacc tcaagagaga
     3061 cgccttgctt tgcaggcacg gacacaggcc tcctggattg ccgcggaggg cagaaataga
     3121 accctggagt cgatcgcaac caccttgagc actcgtcgtg ggcaccatga ctaccgggct
     3181 gccatcatcg cagagaacca tgatgacgct gtgcagaaac tgtctgacat tgtcaatggt
     3241 aaagcagccg aatggacgac gtcgagtcgt gttctcgatg ccagttgctc caaggacgtg
     3301 gtgtgggttt tctccggtca tggcgcacaa tggactgcaa tggctacgga tctcctcaaa
     3361 gacattgtgt tctatcaaac aatcagccgt ctggacccga ttgtggagcg cgaaatgggc
     3421 ttctcggcat tgcattccct tgcaagtggc gatttcgaat cgtccatcaa ggtgcaagtg
     3481 ctcacctatc tcgtacaggt gggactggct gccatcttgc gctcgaaggg actggagccc
     3541 caggctgtca tcggtcattc agttggcgaa attgccgcct cagtcgcggc cggctgtctg
     3601 actgcagaag aaggcgccct gattgtcacc cgcagagcaa acctctatcg gcgtgtgatg
     3661 ggcgcgggcg caatggttct cgtcaacatt ccattcgccg acatggagaa agagcttcaa
     3721 ggccggacgg acctggtggc cgccattgac tcctcgccgt cttcatgtgt tgtttccggt
     3781 gccactgaag ctgtcctggc gctcgtggaa gacctcaagt ctcgtggtgt caacgctttc
     3841 cgggtcaaga cggatattcc cttccaccac ccgatgctgg atcagctgtc cgagccattg
     3901 cgagaggcca tggaagggtc cctgtcgcca cgcaagccca gagtccgtct ttactcgacg
     3961 tcggcagaag acccacgcag tatggttgct cgggatatcc attactggac cagcaacatg
     4021 gtcaacccgg tccggttgac ggccgcagtg caggcagcag tggacgatgg cctgcgactg
     4081 ttccttgaag tctcttctca tcccattgtg tctcactccg tccgagagac catgttggac
     4141 ctgggtgtgg aggacttcac cgtgaccaac accatggctc gcaataagcc tgccgacaag
     4201 actattctgt ccagcattgc ccagcttcac tgtcggggcg ccgtcgtcaa ttggaagaag
     4261 cagctgccgg gcccttgggc gctggatgtg cccttgacga cgtgggacca caagcccttc
     4321 tggcggcata ttcacactgg ccctatcagt gcctcgactt tgcatgatgt ggacaaacac
     4381 acgctgttgg gtcagcgcgt tcccgttgcg ggagaaacga ctatggtgtt caccacccaa
     4441 atggatgacc agaccaagcc tttcccagga agccatccac tgcacggctc tgagattgtt
     4501 ccggctgctg cccttgtcaa cactttcttg catgccacca gggctaccac cctttccaac
     4561 attactcttc gcgtcccagt ggccatcagc cagccgcgcg acatccaggt ggtggtgtca
     4621 cagaatcaaa tcaagatctg ctcccgtctc actcagaagg cggattctgg ggcagacgaa
     4681 ggttcctggc tgacacacac tacgggtcag tgggaagctg gtggaagcaa gaaccccccg
     4741 gcgcaactcg atattgctgc tatcaaggct cgtctcgcta ataacaagtt ggcggacaac
     4801 ttctccatcg actatttgga caaggttggc gtttcggcca tgggcttccc ttgggcagtt
     4861 acagagcact acggcaccct gcaggagatg atcgctcgcg ttgatgtcgc gccagacgtc
     4921 cccgcgacca gtccactccc ctgggatgct gcctcttggg ccccgatcct cgatgcggcc
     4981 acctcagtgg gatccacgct ctttttcgat cagcctcgcc tgcgcatgcc ggctcacatt
     5041 cacggggttc aagtctacac cacgcagccg cctcccaagg tgggttacct gtacgtggaa
     5101 aaggctggcg atcgggatct ggcggtgcat gtcagtgtct gcgacgagct cggaaccgtc
     5161 ttagctcgat tcgaatccat gcgcttttcc gagatcgaag gcacgccggg cagtaacggc
     5221 agcgaggaga gtcttgtcca ccagctcgca tggcctcccg ccacctacag cgagaagccg
     5281 ctgacaatca acaatgtcgt cctcatttcc cgggatagga acgtcgcaga tctctactgt
     5341 gggtctttga aagatcgtgt gtcatctatc acggtgctgg atgctgctgc cgacctgctt
     5401 tccctttcgc aggatcccag cagtgtcttg caagcaaagg atacagcggt ggtgtacgtg
     5461 cccggtcccc tccacagcgc ggattctatc ccgactgcgg cccattcctt cctcatggaa
     5521 ttgctcctcc tggtcaaaat cattgtcaat ggctctttgc ccaccaaggt ctttgtcctt
     5581 acggaccgcg tctgcgagag tgagtctgcg acggctctcg ctcagtctcc gatccacggt
     5641 gtctcccgca tcattgcttc ggagcaccca gatcaatggg gcggactgat tgacgtcgaa
     5701 acgccgggcc agttcccact cgagacgatg aagtatgtgc aggaggcgga caacatccgc
     5761 atctcggatg gcatacccag aattgctcgt ctgcgcccgc ttcctcgcga caagctccta
     5821 ccgcctagca agcagacttc cctgcttccc cggcccgaag gtacctactt aattacgggt
     5881 ggactgggcg ctctggggtt ggaggtcgca cagttcctgg tggaaaaggg tgctcgtcga
     5941 ttgatcctcg tttctcggcg tgccttgcct ccgcgccggg agtgggcaga catccttgct
     6001 gatccatcgt cctcgctggc gccggcgctg gagacaatcc aggcccttga gacacaggga
     6061 gccactgtcc acaccctcgc agtggacatt tcctctcctg acgcagcgcc tcaactggca
     6121 gtcgccattg atgctctgtc gctacctcca gtccgcggcg tggtccacgc agcaggcgtt
     6181 ctggacagcc agctggtcct ctccgccacg tcagactctg tcgagcgcgt gctggcgccc
     6241 aagatcaccg gagcgctggt ccttggcacc gtcttccccc ccaaggcact cgatttcttc
     6301 atgctattct cctcatgcgg acagatacta ggcttcccag gtcaagcatc ctacgcgtct
     6361 ggaaacgcgt tccttgatgc attcgcaaca tcgcgccgac accaaggaga caacgctgtc
     6421 gccgtgcagt ggaccagctg gcgctccctc ggcatggcag ccagtaccga cttcatcaac
     6481 gctgagctag ccagcaaggg catcactgac atcactcgcg acgaggggtt ccgcgcgtgg
     6541 atgcatattt ccaaatatga tatcgaccag gccgccgtct tgcgcagtct ggccttcgag
     6601 gccgatgaac ccctccccac ccctatcctt acggatattg ccgtccgcaa ggctggctcc
     6661 gcctcctccg ctgatgctcc ctctgctgca ccgaaagaga cgaacgaaat gccggaatcg
     6721 atcccggagc gtcgtacctg gttggatgaa cgaatccgtg attgtgtggc ccgcgtgctt
     6781 cagctgggga gcagcgatga ggttgattcc aaggccgctc tgagtgatct gggagtcgac
     6841 agcgtcatga ccgttagctt gagaggtcag ctgcagaaga cgttgggggt caaggtgcca
     6901 cccacactga cctggagttg cccgacggtg tcacatctgg tgggatggtt tttggaaaag
     6961 atgggaaagg cggccgccag ctttctacat catcatcatc atcattgagt ttgtagcctt
     7021 agacatgact gttcctcagt tcaagttggg cacttacgag aagaccggtc ttgctagatt
     7081 ctaatcaaga ggatgtcaga atgccatttg cctgagagat gcaggcttca tttttgatac
     7141 ttttttattt gtaacctata tagtatagga ttttttttgt cattttgttt cttctcgtac
     7201 gagcttgctc ctgatcagcc tatctcgcag ctgatgaata tcttgtggta ggtgtttggg
     7261 aaaatcattc gagtttgatg tttttcttgg tatttcccac tcctcttcag agtacagaag
     7321 attaagtgag atttttgtag aaatgtcttg gtgtcctcgt ccaatcaggt agccatctct
     7381 gaaatatctg gctccgttgc aactccgaac gacctgctgg caacgtaaaa ttctccgggg
     7441 taaaacttaa atgtggagta atggaaccag aaacgtctct tcccttctct ctccttccac
     7501 cgcccgttac cgtccctagg aaattttact ctgctggaga gcttcttcta cggccccctt
     7561 gcagcaatgc tcttcccagc attacgttgc gggtaaaacg gaggtcgtgt acccgaccta
     7621 gcagcccagg gatggaaaag tcccggccgt cgctggcaat aatagcgggc ggacgcatgt
     7681 catgagatta ttggaaacca ccagaatcga atataaaagg cgaacacctt tcccaatttt
     7741 ggtttctcct gacccaaaga ctttaaattt aatttatttg tccctatttc aatcaattga
     7801 acaactattt cgaaacgagg accatgggta tggtgcaaga cacatcaagc gcaagcactt
     7861 cgccaatttt aacaagatgg tacatcgaca cccgccctct aaccgcctca acagcagccc
     7921 ttcctctcct tgaaaccctc cagcccgctg atcaaatctc cgtccaaaaa tactaccatc
     7981 tgaaggataa acacatgtct ctcgcctcta atctgctcaa atacctcttc gtccaccgaa
     8041 actgtcgcat cccctggtct tcaatcgtga tctctcgaac cccagatccg cacagacgac
     8101 catgctatat tccaccctca ggctcacagg aagacagctt caaagacgga tataccggca
     8161 tcaacgttga gttcaacgtc agccaccaag cctcaatggt cgcgatcgcg ggaacagctt
     8221 ttactcccaa tagtggtggg gacagcaaac tcaaacccga agtcggaatt gatattacgt
     8281 gcgtaaacga gcggcaggga cggaacgggg aagagcggag cctggaatcg ctacgtcaat
     8341 atattgatat attctcggaa gtgttttcca ctgcagagat ggccaatata aggaggttag
     8401 atggagtctc atcatcctca ctgtctgctg atcgtcttgt ggactacggg tacagactct
     8461 tctacactta ctgggcgctc aaagaggcgt atataaaaat gactggggag gccctcttag
     8521 caccgtggtt acgggaactg gaattcagta atgtcgtcgc cccggccgct gttgcggaga
     8581 gtggggattc ggctggggat ttcggggagc cgtatacggg tgtcaggacg actttatata
     8641 aaaatctcgt tgaggatgtg aggattgaag ttgctgctct gggcggtgat tacctatttg
     8701 caacggctgc gaggggtggt gggattggag ctagttctag accaggaggt ggtccagacg
     8761 gaagtggtat ccgaagccag gatccctgga ggcctttcaa gaagttagat atagagcgag
     8821 atatccagcc ctgtgcgact ggggtgtgta attgcctatc ccatcatcat catcatcatt
     8881 gacctagggc ggccgcgaat taattcgcct tagacatgac tgttcctcag ttcaagttgg
     8941 gcacttacga gaagaccggt cttgctagat tctaatcaag aggatgtcag aatgccattt
     9001 gcctgagaga tgcaggcttc atttttgata cttttttatt tgtaacctat atagtatagg
     9061 attttttttg tcattttgtt tcttctcgta cgagcttgct cctgatcagc ctatctcgca
     9121 gctgatgaat atcttgtggt aggggtttgg gaaaatcatt cgagtttgat gtttttcttg
     9181 gtatttccca ctcctcttca gagtacagaa gattaagtga gacgttcgtt tgtgcaagct
     9241 tatcgataag ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc
     9301 gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt
     9361 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga
     9421 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg
     9481 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc
     9541 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcta
     9601 tcgaatctaa atgtaagtta aaatctctaa ataattaaat aagtcccagt ttctccatac
     9661 gaaccttaac agcattgcgg tgagcatcta gaccttcaac agcagccaga tccatcactg
     9721 cttggccaat atgtttcagt ccctcaggag ttacgtcttg tgaagtgatg aacttctgga
     9781 aggttgcagt gttaactccg ctgtattgac gggcatatcc gtacgttggc aaagtgtggt
     9841 tggtaccgga ggagtaatct ccacaactct ctggagagta ggcaccaaca aacacagatc
     9901 cagcgtgttg tacttgatca acataagaag aagcattctc gatttgcagg atcaagtgtt
     9961 caggagcgta ctgattggac atttccaaag cctgctcgta ggttgcaacc gatagggttg
    10021 tagagtgtgc aatacacttg cgtacaattt caacccttgg caactgcaca gcttggttgt
    10081 gaacagcatc ttcaattctg gcaagctcct tgtctgtcat atcgacagcc aacagaatca
    10141 cctgggaatc aataccatgt tcagcttgag acagaaggtc tgaggcaacg aaatctggat
    10201 cagcgtattt atcagcaata actagaactt cagaaggccc agcaggcatg tcaatactac
    10261 acagggctga tgtgtcattt tgaaccatca tcttggcagc agtaacgaac tggtttcctg
    10321 gaccaaatat tttgtcacac ttaggaacag tttctgttcc gtaagccata gcagctactg
    10381 cctgggcgcc tcctgctagc acgatacact tagcaccaac cttgtgggca acgtagatga
    10441 cttctggggt aagggtacca tccttcttag gtggagatgc aaaaacaatt tctttgcaac
    10501 cagcaacttt ggcaggaaca cccagcatca gggaagtgga aggcagaatt gcggttccac
    10561 caggaatata gaggccaact ttctcaatag gtcttgcaaa acgagagcag actacaccag
    10621 ggcaagtctc aacttgcaac gtctccgtta gttgagcttc atggaatttc ctgacgttat
    10681 ctatagagag atcaatggct ctcttaacgt tatctggcaa ttgcataagt tcctctggga
    10741 aaggagcttc taacacaggt gtcttcaaag cgactccatc aaacttggca gttagttcta
    10801 aaagggcttt gtcaccattt tgacgaacat tgtcgacaat tggtttgact aattccataa
    10861 tctgttccgt tttctggata ggacgacgaa gggcatcttc aatttcttgt gaggaggcct
    10921 tagaaacgtc aattttgcac aattcaatac gaccttcaga agggacttct ttaggtttgg
    10981 attcttcttt aggttgttcc ttggtgtatc ctggcttggc atctcctttc cttctagtga
    11041 cctttaggga cttcatatcc aggtttctct ccacctcgtc caacgtcaca ccgtacttgg
    11101 cacatctaac taatgcaaaa taaaataagt cagcacattc ccaggctata tcttccttgg
    11161 atttagcttc tgcaagttca tcagcttcct ccctaatttt agcgttcaac aaaacttcgt
    11221 cgtcaaataa ccgtttggta taagaacctt ctggagcatt gctcttacga tcccacaagg
    11281 tggcttccat ggctctaaga ccctttgatt ggccaaaaca ggaagtgcgt tccaagtgac
    11341 agaaaccaac acctgtttgt tcaaccacaa atttcaagca gtctccatca caatccaatt
    11401 cgatacccag caacttttga gttgctccag atgtagcacc tttataccac aaaccgtgac
    11461 gacgagattg gtagactcca gtttgtgtcc ttatagcctc cggaatagac tttttggacg
    11521 agtacaccag gcccaacgag taattagaag agtcagccac caaagtagtg aatagaccat
    11581 cgtggcggtc agtagtcaaa gacgccaaca aaatttcact gacagggaac tttttgacat
    11641 cttcagaaag ttcgtattca gtagtcaatt gccgagcatc aataatgggg attataccag
    11701 aagcaacagt ggaagtcaca tctaccaact ttgcggtctc agaaaaagca taaacagttc
    11761 tactaccgcc attagtgaaa cttttcaaat cgcccagtgg agaagaaaaa ggcacagcga
    11821 tactagcatt agcgggcaag gatgcaactt tatcaaccag ggtcctatag ataaccctag
    11881 cgcctgggat catcctttgg acaactcttt ctgccaaatc taggtccaaa atcacttcat
    11941 tgataccatt attgtacaac ttgagcaagt tgtcgatcag ctcctcaaat tggtcctctg
    12001 taacggatga ctcaacttgc acattaactt gaagctcagt cgattgagtg aacttgatca
    12061 ggttgtgcag ctggtcagca gcatagggaa acacggcttt tcctaccaaa ctcaaggaat
    12121 tatcaaactc tgcaacactt gcgtatgcag gtagcaaggg aaatgtcata cttgaagtcg
    12181 gacagtgagt gtagtcttga gaaattctga agccgtattt ttattatcag tgagtcagtc
    12241 atcaggagat cctctacgcc ggacgcatcg tggccgacct gcaggggggg gggggcgctg
    12301 aggtctgcct cgtgaagaag gtgttgctga ctcataccag gcctgaatcg ccccatcatc
    12361 cagccagaaa gtgagggagc cacggttgat gagagctttg ttgtaggtgg accagttggt
    12421 gattttgaac ttttgctttg ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg
    12481 atccttcaac tcagcaaaag ttcgatttat tcaacaaagc cgccgtcccg tcaagtcagc
    12541 gtaatgctct gccagtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc
    12601 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
    12661 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
    12721 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
    12781 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
    12841 aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
    12901 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
    12961 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
    13021 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
    13081 gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
    13141 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
    13201 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
    13261 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
    13321 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc
    13381 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
    13441 gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg
    13501 tggctttccc ccccccccct gcaggtcggc atcaccggcg ccacaggtgc ggttgctggc
    13561 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
    13621 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
    13681 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
    13741 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag tatctatgat tggaagtatg
    13801 ggaatggtga tacccgcatt cttcagtgtc ttgaggtctc ctatcagatt atgcccaact
    13861 aaagcaaccg gaggaggaga tttcatggta aatttctctg acttttggtc atcagtagac
    13921 tcgaactgtg agactatctc ggttatgaca gcagaaatgt ccttcttgga gacagtaaat
    13981 gaagtcccac caataaagaa atccttgtta tcaggaacaa acttcttgtt tcgaactttt
    14041 tcggtgcctt gaactataaa atgtagagtg gatatgtcgg gtaggaatgg agcgggcaaa
    14101 tgcttacctt ctggaccttc aagaggtatg tagggtttgt agatactgat gccaacttca
    14161 gtgacaacgt tgctatttcg ttcaaaccat tccgaatcca gagaaatcaa agttgtttgt
    14221 ctactattga tccaagccag tgcggtcttg aaactgacaa tagtgtgctc gtgttttgag
    14281 gtcatctttg tatgaataaa tctagtcttt gatctaaata atcttgacga gccagacgat
    14341 aataccaatc taaactcttt aaacgttaaa ggacaagtat gtctgcctgt attaaacccc
    14401 aaatcagctc gtagtctgat cctcatcaac ttgaggggca ctatcttgtt ttagagaaat
    14461 ttgcggagat gcgatatcga gaaaaaggta cgctgatttt aaacgtgaaa tttatctcaa
    14521 gatctgctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    14581 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    14641 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
    14701 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    14761 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
    14821 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    14881 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    14941 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    15001 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    15061 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    15121 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    15181 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    15241 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    15301 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    15361 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    15421 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    15481 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    15541 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    15601 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    15661 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    15721 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    15781 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    15841 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    15901 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    15961 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    16021 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
    16081 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    16141 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    16201 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    16261 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    16321 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
    16381 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    16441 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    16501 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    16561 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    16621 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    16681 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    16741 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    16801 aggccc
//
