LOCUS       sequence-246633-        8061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion 
            of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT
            core (aa1048-1664), and Flag tag epitope driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 8061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pcDNA-LmoCas6-p300
FEATURES             Location/Qualifiers
     source          1..8061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      904..913
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             913..936
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             943..963
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1696..1743
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             1750..3600
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      3364..3373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3607..3630
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3652..3669)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3658..3882
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3928..4356
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4015..4034)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4225..4246
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4365..4385)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4370..4699
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4550..4685
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4612..4631
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4766..5560
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4820..4839)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5430..5449
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5734..5855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5771..5790)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5825..5844
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5904..5920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5904..5920)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5917..5939)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5928..5944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5952..5958,5959..5976,5977..5982))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5997..6018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6135..6152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6306..6894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6386..6405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7065..7856,7857..7925))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7688..7707
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7926..8030)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccgccacca tggactacaa ggacgacgac gataagatgg cgccaaagaa aaagcgcaag
      961 gtccgcggaa tgcggctgaa gattaactgc gacttcgaca gcaagatcat ccccaaggac
     1021 ttccagtcca aggtcgtgtc cctgttcaaa gccggaatta tgaaaagctc cccggagaga
     1081 tacgagaacc tgtttggtgg aaacaagcac aagcagtaca ctttctcggt gtatctccca
     1141 aaacctcaga acaagggcgc cgaaattcag ctgaacgagg cgaactgtat catcaacttc
     1201 tccaccggcg atgctgaaac cggtattatc ttctacaacg ccctgattag cctgagaggc
     1261 tccaaggtcc tgtttggggc cggaaatcac atcaccgtga agaacatcca gatcgtgcct
     1321 gagaagaaga tcatcgggaa gcaaaccatt ctgcggaccc tttcgccggt ggtgtcgagg
     1381 gatcacaacc gggaaacctt caagaattgg ttctactcat tcgaggacga agaattcgag
     1441 cctactttga agcggaacat gctgccgtac ctcatcgacg cctttggaga acaggctcgc
     1501 tacgatctgg aaaagctgaa gatcacgccc atttccatga agaaagtcgt ggtgtactgc
     1561 catgagatcc acatcgagag ctccgtgggc atcttcgagc tggaagccga ggcatacctc
     1621 caaaagtacc tcgtggaaaa cggaatcggc actatgactg gctcagggtt cggaatgatt
     1681 gaacagttcc ggggcaagcg acctgccgcc acaaagaagg ctggacaggc taagaagaag
     1741 aaaggatcca ttttcaaacc agaagaacta cgacaggcac tgatgccaac tttggaggca
     1801 ctttaccgtc aggatccaga atcccttccc tttcgtcaac ctgtggaccc tcagctttta
     1861 ggaatccctg attactttga tattgtgaag agccccatgg atctttctac cattaagagg
     1921 aagttagaca ctggacagta tcaggagccc tggcagtatg tcgatgatat ttggcttatg
     1981 ttcaataatg cctggttata taaccggaaa acatcacggg tatacaaata ctgctccaag
     2041 ctctctgagg tctttgaaca agaaattgac ccagtgatgc aaagccttgg atactgttgt
     2101 ggcagaaagt tggagttctc tccacagaca ctgtgttgct acggcaaaca gttgtgcaca
     2161 atacctcgtg atgccactta ttacagttac cagaacaggt atcatttctg tgagaagtgt
     2221 ttcaatgaga tccaagggga gagcgtttct ttgggggatg acccttccca gcctcaaact
     2281 acaataaata aagaacaatt ttccaagaga aaaaatgaca cactggatcc tgaactgttt
     2341 gttgaatgta cagagtgcgg aagaaagatg catcagatct gtgtccttca ccatgagatc
     2401 atctggcctg ctggattcgt ctgtgatggc tgtttaaaga aaagtgcacg aactaggaaa
     2461 gaaaataagt tttctgctaa aaggttgcca tctaccagac ttggcacctt tctagagaat
     2521 cgtgtgaatg actttctgag gcgacagaat caccctgagt caggagaggt cactgttaga
     2581 gtagttcatg cttctgacaa aaccgtggaa gtaaaaccag gcatgaaagc aaggtttgtg
     2641 gacagtggag agatggcaga atcctttcca taccgaacca aagccctctt tgcctttgaa
     2701 gaaattgatg gtgttgacct gtgcttcttt ggcatgcatg ttcaagagta tggctctgac
     2761 tgccctccac ccaaccagag gagagtatac atatcttacc tcgatagtgt tcatttcttc
     2821 cgtcctaaat gcttgaggac tgcagtctat catgaaatcc taattggata tttagaatat
     2881 gtcaagaaat taggttacac aacagggcat atttgggcat gtccaccaag tgagggagat
     2941 gattatatct tccattgcca tcctcctgac cagaagatac ccaagcccaa gcgactgcag
     3001 gaatggtaca aaaaaatgct tgacaaggct gtatcagagc gtattgtcca tgactacaag
     3061 gatattttta aacaagctac tgaagataga ttaacaagtg caaaggaatt gccttatttc
     3121 gagggtgatt tctggcccaa tgttctggaa gaaagcatta aggaactgga acaggaggaa
     3181 gaagagagaa aacgagagga aaacaccagc aatgaaagca cagatgtgac caagggagac
     3241 agcaaaaatg ctaaaaagaa gaataataag aaaaccagca aaaataagag cagcctgagt
     3301 aggggcaaca agaagaaacc cgggatgccc aatgtatcta acgacctctc acagaaacta
     3361 tatgccacca tggagaagca taaagaggtc ttctttgtga tccgcctcat tgctggccct
     3421 gctgccaact ccctgcctcc cattgttgat cctgatcctc tcatcccctg cgatctgatg
     3481 gatggtcggg atgcgtttct cacgctggca agggacaagc acctggagtt ctcttcactc
     3541 cgaagagccc agtggtccac catgtgcatg ctggtggagc tgcacacgca gagccaggac
     3601 ggatccgatt acaaagacga tgacgataag tgagtttaaa ccgctgatca gcctcgactg
     3661 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3721 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3781 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     3841 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     3901 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     3961 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4021 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4081 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4141 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4201 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4261 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4321 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     4381 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4441 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4501 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4561 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4621 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     4681 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     4741 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4801 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4861 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     4921 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     4981 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5041 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     5101 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     5161 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     5221 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     5281 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     5341 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5401 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5461 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5521 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     5581 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     5641 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     5701 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     5761 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5821 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     5881 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5941 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6001 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     6061 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6121 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6181 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6421 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6481 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6541 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6601 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6661 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6721 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6781 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6841 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6901 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6961 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7021 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7081 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7141 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7201 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7261 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7321 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7381 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7441 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7501 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7561 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7621 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7681 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7741 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7801 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7861 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7921 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7981 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8041 cgaaaagtgc cacctgacgt c
//
