LOCUS       sequence-246714-       11124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B 
            crRNA targeting HBE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11124)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 11124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pLenti-LmoCascade-HBE1crRNA
FEATURES             Location/Qualifiers
     source          1..11124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     329..348
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        423..527
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(528..596,597..1388)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(746..765)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1559..2147
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2048..2067
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2301..2318
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2435..2456
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2471..2476,2477..2494,2495..2501)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2509..2525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2514..2536
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2533..2549
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2598..2719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2609..2628)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2663..2682
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2849..3223)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3242..3289)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(3337..3666)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3351..3486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3405..3424)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3651..3671
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3680..4108)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3790..3811)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4002..4021
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    4154..4378
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4367..4384
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             4410..4590
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     5095..5111
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5096..5112
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5115..5703
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5221..5232
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5630..5650
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    5728..5761
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5774..6373)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5857..5877)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6354..6373
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(6374..6430)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(join(6440..7150,7151..7153,7154..7156))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6440..7150,7151..7153,7154..7156))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6482..6503)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6831..6850
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7090..7111
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(7185..8396)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(7329..7348)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     8424..8441
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8425..8441
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8451..8470
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8451..8469
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        8484..8732
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8484..8504
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8655..8674
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(8862..8882)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8862..8880)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8901..8917)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8962..9079
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9377..9421)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9606..9839
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10332..10457
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10504..10684
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(10702..10900)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(10727..10747)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        join(10902..11124,1..157)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
       61 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
      121 attgattact attaataact agtcaataat caatgtcaac gcgtatatct ggcccgtaca
      181 tcgcgaagca gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag
      241 ccttgccttg ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa
      301 gcagggagca gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
      361 cataggggat cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg
      421 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      481 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      541 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      601 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      661 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      721 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      781 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      841 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      901 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      961 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1021 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1081 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1141 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1201 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1261 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1321 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1381 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1441 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1501 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1561 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1621 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1681 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1741 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1801 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1861 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1921 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1981 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2041 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2101 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2161 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2221 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2281 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2341 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2401 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     2461 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     2521 aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg
     2581 acggtataca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     2641 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     2701 aagctgcaat aaacaagttg gggtgggcga agaactccag catgagatcc ccgcgctgga
     2761 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
     2821 cggtggaatc gaaatctcgt agcacgtgtc agtcctgctc ctcggccacg aagtgcacgc
     2881 agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg ccgatctcgg
     2941 tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac cactcggcgt
     3001 acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccggc accacctggt
     3061 cctggaccgc gctgatgaac agggtcacgt cgtcccggac cacaccggcg aagtcgtcct
     3121 ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt
     3181 cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catggtttag ttcctcacct
     3241 tgtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaca cgtgctgatc
     3301 agatccgaaa atggatatac aagctcccgg gagctttttg caaaagccta ggcctccaaa
     3361 aaagcctcct cactacttct ggaatagctc agaggcagag gcggcctcgg cctctgcata
     3421 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     3481 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     3541 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     3601 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     3661 acacacattc cacagaatta attcgcgtta aatttttgtt aaatcagctc attttttaac
     3721 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     3781 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     3841 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     3901 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     3961 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     4021 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     4081 gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc tagagcccca gctggttctt
     4141 tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
     4201 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
     4261 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
     4321 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc
     4381 gaggctgatc agcgggttta aacgggccct gctagagatt ttccacactg actaaaaggg
     4441 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     4501 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     4561 ggctcagatc tggtctaacc agagagaccc agtacagtcc ggatgcagct ctcgggccat
     4621 gtgatgaaat gctaggcggc tgtcaaacct ccactctaat acttctctct ccgggtcatc
     4681 catcccatgc aggctcacag ggtgtaacaa gcgggtgttc tctccttcat tggcttcttc
     4741 taccttctct tgctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct
     4801 gatccctggc cctggtgtgt agttctgcca atcagggaag tagccttgtg tgtggtagat
     4861 ccacagatca aggatatctt gtcttcgttg ggagtgaatt agcccttcca gtcccccctt
     4921 ttcttttaaa aagtggctaa gatctacagc tgccttgtaa gtcattggtc ttaaaggtac
     4981 ctgaggtgtg actggaaaac ccacctcctc ctcctcttgt gcttctagcc aggcacaatc
     5041 agcattggta gctgctgtat tgctacttgt gattgctcca tgtttttcta ggtctcgagg
     5101 tcgacggtat cgatgcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
     5161 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
     5221 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca
     5281 ggcgagcagc caaggaaagg acgatgattt ccccgacaac accacggaat tgtcagtgcc
     5341 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
     5401 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
     5461 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
     5521 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
     5581 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
     5641 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
     5701 attatcgata agcttgatat cgaattcata acttcgtata atgtatgcta tacgaagtta
     5761 tctattatca actttaggca ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg
     5821 gcacctcgac gtcggcggtg acggtgaagc cgagccgctc gtagaagggg aggttgcggg
     5881 gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc actccgggga
     5941 gcacgacggc gctgcccaga cccttgccct ggtggtcggg cgacacgccg acggtggcca
     6001 ggaaccacgc gggctccttg ggccggtgcg gcgccaggag gccttccatc tgttgctgcg
     6061 cggccagccg ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg aacaccgccc
     6121 ccgcttcgac gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc
     6181 acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc
     6241 gctcgatgtg gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg
     6301 cggcgagggt gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc accgtgggct
     6361 tgtactcggt catcggtcca ggattctctt cgacatctcc ggcttgtttc agcagagaga
     6421 agtttgttgc gccggatccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     6481 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
     6541 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     6601 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     6661 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
     6721 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     6781 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     6841 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     6901 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
     6961 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
     7021 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
     7081 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
     7141 cgcccttgct caccattcta gagtcgacct gcagcccaag cttcgtctaa caaaaaagcc
     7201 aaaaacggcc agaatttagc ggacaattta ctagtctaac actgaaaatt acatattgac
     7261 ccaaatgatt acatttcaaa aggtgcctaa aaaacttcac aaaacacact cgccaacccc
     7321 gagcgcatag ttcaaaaccg gagcttcagc tacttaagaa gataggtaca taaaaccgac
     7381 caaagaaact gacgcctcac ttatccctcc cctcaccaga ggtccggcgc ctgtcgattc
     7441 aggagagcct accctaggcc cgaaccctgc gtcctgcgac ggagaaaagc ctaccgcaca
     7501 cctaccggca ggtggcccca ccctgcatta taagccaaca gaacgggtga cgtcacgaca
     7561 cgacgagggc gcgcgctccc aaaggtacgg gtgcactgcc caacggcacc gccataactg
     7621 ccgcccccgc aacagacgac aaaccgagtt ctccagtcag tgacaaactt cacgtcaggg
     7681 tccccagatg gtgccccagc ccatctcacc cgaataagag ctttcccgca ttagcgaagg
     7741 cctcaagacc ttgggttctt gccgcccacc atgcccccca ccttgtttca acgacctcac
     7801 agcccgcctc acaagcgtct tccattcaag actcgggaac agccgccatt ttgctgcgct
     7861 ccccccaacc cccagttcag ggcaaccttg ctcgcggacc cagactacag cccttggcgg
     7921 tctctccaca cgcttccgtc ccaccgagcg gcccggcggc cacgaaagcc ccggccagcc
     7981 cagcagcccg ctactcacca agtgacgatc acagcgatcc acaaacaaga accgcgaccc
     8041 aaatcccggc tgcgacggaa ctagctgtgc cacacccggc gcgtccttat ataatcatcg
     8101 gcgttcaccg ccccacggag atccctccgc agaatcgccg agaagggact acttttcctc
     8161 gcctgttccg ctctctggaa agaaaaccag tgccctagag tcacccaagt cccgtcctaa
     8221 aatgtccttc tgctgatact ggggttctaa ggccgagtct tatgagcagc gggccgctgt
     8281 cctgagcgtc cgggcggaag gatcaggacg ctcgctgcgc ccttcgtctg acgtggcagc
     8341 gctcgccgtg aggagggggg cgcccgcggg aggcgccaaa acccggcgcg gaggccagat
     8401 ctggagccga cacgggttaa tgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     8461 cactataggg cgaattgggt accgagggcc tatttcccat gattccttca tatttgcata
     8521 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     8581 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     8641 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     8701 ctttatatat cttgtggaaa ggacgaaaca ccgcggaccg gttttaacta cttattatga
     8761 aatgtaaatg gcctgagagc ttgctagtga ttgcagctgt gtccggtttt aactacttat
     8821 tatgaaatgt aaattttttt tccgagctcc agcttttgtt ccctttagtg agggttaatt
     8881 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt attaaccaaa ctggatctct
     8941 gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt
     9001 agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat
     9061 cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtctgcaga attggcgcac
     9121 gcagtgccga tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt
     9181 cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata
     9241 atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa actattctta
     9301 aacctaccaa gcctcctact atcattatga ataattttat ataccacagc caatttgtta
     9361 tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct tgttcattct
     9421 tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa
     9481 tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac
     9541 tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa
     9601 tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct
     9661 gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca
     9721 aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca
     9781 ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta
     9841 ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc
     9901 ctaatggttc aatttttact actttatatt tatataattc acttctccaa ttgtccctca
     9961 tatctcctcc tccaggtctg aagatcagcg gccgcttgct gtgcggtggt cttacttttg
    10021 ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga
    10081 tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc
    10141 tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg
    10201 ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta
    10261 tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc
    10321 cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt
    10381 tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga
    10441 gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc
    10501 cactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
    10561 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
    10621 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
    10681 acccagtaca ggcaaaacgc gctgcttata tagacctccc accgtacacg cctaccgccc
    10741 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
    10801 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
    10861 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
    10921 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
    10981 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
    11041 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
    11101 gtccctattg gcgttactat ggga
//
