LOCUS       sequence-246718-        9529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Levy C, Khaled M, Iliopoulos D, Janas MM, Schubert S, Pinner S, Chen
            PH, Li S, Fletcher AL, Yokoyama S, Scott KL, Garraway LA, Song JS, 
            Granter SR, Turley SJ, Fisher DE, Novina CD
  TITLE     Intronic miR-211 assumes the tumor suppressive function of its host 
            gene in melanoma.
  JOURNAL   Mol Cell. 2010 Dec 10. 40(5):841-9.
  PUBMED    21109473
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2010 Dec 10. 40(5):841-9."
COMMENT     Sequence Label: PLKO-mcherry-luc-puro-shSCR
FEATURES             Location/Qualifiers
     source          1..9529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2014..2262
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2014..2034
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2185..2204
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2359..2476
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2525..3035
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2954..2972
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      3044..3053
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4709..4768
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     primer_bind     complement(4920..4938)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             5498..5551
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     LTR             6229..6462
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6534..6655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6571..6590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6625..6644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6695..6830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6850..6869)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6851..6869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6879..6896)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6879..6895)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6888..6910)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7037..7492
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7124..7143)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7334..7355
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7518..7622
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7623..7691,7692..8483)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7841..7860)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8654..9242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9143..9162
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9396..9413
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca
     1981 cgagactagc ctcgagcggc cgcccccttc accgagggcc tatttcccat gattccttca
     2041 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
     2101 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     2161 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     2221 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggaattct ccgaacgtgt
     2281 cacgtctcga gacgtgacac gttcggagaa tttttttgaa ttctcgacct cgagacaaat
     2341 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     2401 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     2461 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct
     2521 cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     2581 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     2641 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     2701 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     2761 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     2821 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     2881 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     2941 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     3001 ccctcgttga ccgaatcacc gacctctctc cccaggggga tccgccacca tggaagatgc
     3061 caagaacatc aagaaaggcc ctgccccctt ctaccccctg gaagatggca cagccggcga
     3121 gcagctgcac aaggccatga agagatacgc cctcgtccct ggaacaatcg ccttcaccga
     3181 cgcccacatc gaggtggaca tcacctacgc cgagtacttc gagatgagcg tgcggctggc
     3241 cgaggccatg aaacgctacg gcctgaacac caaccaccgg atcgtggtgt gcagcgagaa
     3301 cagcctgcag ttcttcatgc ccgtgctggg cgccctgttc atcggcgtgg ccgtggcccc
     3361 tgccaacgac atctacaacg agcgggagct gctgaacagc atgggcatca gccagcccac
     3421 cgtggtgttc gtgagcaaga agggcctgca gaaaatcctg aacgtgcaga agaagctgcc
     3481 catcatccag aaaatcatca tcatggacag caagaccgac taccagggct tccagagcat
     3541 gtacaccttc gtgaccagcc acctgccccc tggcttcaac gagtacgact tcgtgcccga
     3601 gagcttcgac agggacaaga ccatcgccct gatcatgaac agcagcggca gcaccggcct
     3661 gcctaaaggc gtggccctgc ctcaccggac cgcctgcgtg cggttcagcc acgcccggga
     3721 ccccatcttc ggcaaccaga tcatccccga caccgccatc ctgagcgtgg tgcccttcca
     3781 ccacggcttc ggcatgttca ccaccctggg ctacctgatc tgcggcttcc gggtggtgct
     3841 gatgtatcgg ttcgaggaag agctgttcct gcggagcctg caggactaca agatccagag
     3901 cgccctgctg gtgcccaccc tgttcagctt tttcgccaag agcaccctga tcgacaagta
     3961 cgacctgagc aacctgcacg agatcgccag cggcggagcc cccctgagca aagaagtggg
     4021 cgaagccgtc gccaagcggt tccacctgcc cggcatccgg cagggctatg gcctgaccga
     4081 gaccacaagc gccattctga tcacccccga gggcgacgac aagcctggcg ccgtgggcaa
     4141 ggtggtgcct ttcttcgagg ccaaggtggt ggacctggac accggcaaga ccctgggcgt
     4201 gaaccagcgg ggcgagctgt gcgtgagggg ccccatgatc atgagcggct acgtgaacaa
     4261 ccccgaggcc accaacgccc tgattgacaa ggacggctgg ctgcacagcg gcgacatcgc
     4321 ctactgggac gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaagta
     4381 caagggctac caggtggccc cagccgagct ggaaagcatc ctgctgcagc accccaacat
     4441 cttcgacgcc ggggtggccg gcctgcccga cgatgacgcc ggcgagctgc ctgccgccgt
     4501 ggtggtcctc gaacatggca aaaccatgac cgagaaagaa atcgtggact acgtggccag
     4561 ccaggtgacc accgccaaga agctgagagg cggcgtcgtg tttgtggacg aggtgcccaa
     4621 gggcctgacc ggcaagctgg acgcccggaa gatccgggag atcctgatta aggccaagaa
     4681 gggcggcaag atcgccgtgg gctccggcca gtgcaccaac tacgccctgc tgaagctggc
     4741 cggcgacgtg gagagcaacc ctggcccttt cgaaatggtg tccaagggcg aagaggacaa
     4801 catggccatc atcaaagagt ttatgcggtt caaggtgcac atggaaggca gcgtgaacgg
     4861 ccacgagttc gagatcgagg gcgagggcga aggcaggccc tacgagggca cccagaccgc
     4921 caagctgaag gtgaccaagg gcggacctct gcccttcgcc tgggacatcc tgagccccca
     4981 gttcatgtac ggcagcaagg cctacgtgaa gcaccccgcc gacatccccg actacctgaa
     5041 gctgtccttc cccgagggct tcaagtggga gcgggtgatg aacttcgagg acggcggggt
     5101 ggtgaccgtg acccaggata gctccctgca ggacggcgag ttcatctaca aggtgaaact
     5161 gcggggcacc aacttcccca gcgacggccc cgtgatgcag aaaaagacaa tgggctggga
     5221 ggccagcagc gagcggatgt accccgagga tggcgccctg aagggcgaga tcaagcagcg
     5281 gctgaagctg aaggacggcg gccactacga cgccgaggtg aaaaccacct acaaggccaa
     5341 aaagcccgtg cagctgcctg gcgcctacaa cgtgaacatc aagctggata tcaccagcca
     5401 caacgaggac tacaccatcg tggagcagta cgagcgggcc gagggcagac acagcaccgg
     5461 cggcatggac gagctgtaca agctcagcgg cagcggcgag ggcagaggca gcctgctgac
     5521 ctgcggcgat gtggaggaaa acccaggccc aatgaccgag tacaagccta ccgtgaggct
     5581 ggccacccgg gacgacgtgc ccagagccgt gaggacactg gccgccgcct tcgccgacta
     5641 ccccgccacc cggcacaccg tggaccccga ccggcacatc gagcgggtga ccgagctgca
     5701 ggaactgttc ctgaccagag tgggcctgga tatcggcaaa gtgtgggtgg ccgacgacgg
     5761 agccgccgtg gccgtgtgga ccacccccga gtccgtggag gccggagccg tgtttgccga
     5821 gatcggcccc aggatggccg agctgtccgg cagcaggctg gccgcccagc agcagatgga
     5881 aggcctgctg gcccctcacc ggcccaaaga gcccgcctgg ttcctggcca ccgtgggcgt
     5941 gagccccgac caccagggca agggcctggg cagcgccgtg gtgctgccag gcgtggaagc
     6001 cgccgagagg gccggagtgc ccgccttcct ggaaaccagc gcccccagga acctgccctt
     6061 ctacgagcgg ctgggcttta ccgtgaccgc cgacgtggag gtgccagagg gccccaggac
     6121 ctggtgcatg acccggaagc caggcgcctg agaattgggt acctttaaga ccaatgactt
     6181 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6241 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     6301 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6361 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6421 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6481 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     6541 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6601 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6661 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6721 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6781 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     6841 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     6901 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6961 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     7021 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7081 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7141 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7201 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7261 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7321 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7381 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7441 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     7501 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7561 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7621 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7681 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7741 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7801 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7861 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7921 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7981 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8041 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8101 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8161 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8221 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8281 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8341 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8401 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8461 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8521 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8581 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8641 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8701 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8761 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8821 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8881 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8941 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9001 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9061 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9121 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9181 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9241 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9301 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9361 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9421 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9481 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaat
//
