LOCUS       sequence-246722-        9609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of CD along with reporter RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Basnet H, Tian L, Ganesh K, Huang YH, Macalinao DG, Brogi E, Finley 
            LW, Massague J
  TITLE     Flura-seq identifies organ-specific metabolic adaptations during 
            early metastatic colonization.
  JOURNAL   Elife. 2019 Mar 26;8. pii: 43627. doi: 10.7554/eLife.43627.
  PUBMED    30912515
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-03-26"; volume: "8. pii: 43627. doi"; pages: " 
            10.7554/eLife.43627"
COMMENT     Sequence Label: pCMVtight-RFP-IRES-CD
FEATURES             Location/Qualifiers
     source          1..9609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1714..2028
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1722..1740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1758..1776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1793..1811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1829..1847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1865..1883
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1900..1918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1936..1954
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2001..2025
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2106..2130
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2185..2880
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     misc_feature    2903..3475
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3070..3087)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3297..3316
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      3499..3508
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3987..4011)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4042..4630
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4095..4115)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4513..4524)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4644..5143
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4679..4702)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5076..5095
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5164..5763
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5164..5183)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5660..5680
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5901..6134
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6206..6327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6243..6262)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6297..6316
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6367..6502
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6522..6541)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6523..6541)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6551..6568)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6551..6567)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6560..6582)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6709..7164
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6796..6815)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7006..7027
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7190..7294
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7295..7363,7364..8155)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7513..7532)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8326..8914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8815..8834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9068..9085
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9202..9223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9238..9243,9244..9261,9262..9268)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9276..9292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9281..9303
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9300..9316
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9300..9316
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9335..9355
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9337..9355
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9383..9609
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 attcaaaatt ttatcgatca cgagactagc ctcgagttta ctccctatca gtgatagaga
     1741 acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc
     1801 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     1861 ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac
     1921 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg
     1981 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattcga
     2041 gctcggtacc cggggatcca ccggttcgtc gactagtcca gtgtggtgga attctgcaga
     2101 tatcaacaag tttgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatct
     2161 cgagctcaag cttcgaacgc caccatgagc gagctgatca aggagaacat gcacatgaag
     2221 ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc
     2281 aagccctacg agggcaccca gaccatgaag atcaaggtgg tcgagggcgg ccctctcccc
     2341 ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaagcctt catcaaccac
     2401 acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga
     2461 atcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cttccagaac
     2521 ggctgcatca tctacaacgt caagatcaac ggggtgaact tcccatccaa cggccctgtg
     2581 atgcagaaga aaacacgcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc
     2641 ggcctgagag gccacagcca gatggccctg aagctcgtgg gcgggggcta cctgcactgc
     2701 tccttcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcttc
     2761 cacttcgtgg accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag
     2821 cagcacgaga tggctgtggc caagtactgc gacctcccta gcaaactggg gcacagataa
     2881 ggatccgcgg ccgcaaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa
     2941 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     3001 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     3061 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     3121 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     3181 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     3241 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     3301 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     3361 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     3421 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg
     3481 gccaccgagt acttcgaagc caccatggtg acagggggaa tggcaagcaa gtgggatcag
     3541 aagggtatgg acattgccta tgaggaggcg gccttaggtt acaaagaggg tggtgttcct
     3601 attggcggat gtcttatcaa taacaaagac ggaagtgttc tcggtcgtgg tcacaacatg
     3661 agatttcaaa agggatccgc cacactacat ggtgagatct ccactttgga aaactgtggg
     3721 agattagagg gcaaagtgta caaagatacc actttgtata cgacgctgtc tccatgcgac
     3781 atgtgtacag gtgccatcat catgtatggt attccacgct gtgttgtcgg tgagaacgtt
     3841 aatttcaaaa gtaagggcga gaaatattta caaactagag gtcacgaggt tgttgttgtt
     3901 gacgatgaga ggtgtaaaaa gatcatgaaa caatttatcg atgaaagacc tcaggattgg
     3961 tttgaagata ttggtgagta gtctagaccc agctttcttg tacaaagtgg ttgatatcca
     4021 gcacagtggc ggccgctcga caatcaacct ctggattaca aaatttgtga aagattgact
     4081 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4141 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4201 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4261 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4321 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4381 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     4441 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4501 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4561 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4621 gcctccccgc ctggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     4681 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     4741 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     4801 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     4861 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     4921 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     4981 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     5041 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     5101 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
     5161 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     5221 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     5281 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5341 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     5401 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     5461 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     5521 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     5581 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     5641 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     5701 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     5761 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatct
     5821 cgagggcccg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5881 tttaaaagaa aaggggggac tggaagggct agctcactcc caacgaagac aagatctgct
     5941 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6001 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6061 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6121 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6181 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     6241 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6301 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     6361 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6421 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6481 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     6541 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6601 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6661 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     6721 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6781 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6841 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6901 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6961 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7021 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7081 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7141 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7201 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7261 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7321 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7381 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7441 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7501 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7561 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7621 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7681 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7741 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7801 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7861 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7921 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7981 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8041 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8101 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8161 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8221 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8281 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8341 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8401 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8461 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8521 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8581 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8641 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8701 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8761 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8821 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8881 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8941 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9001 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9061 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9121 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     9181 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     9241 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9301 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     9361 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     9421 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     9481 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     9541 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     9601 tacataaac
//
