LOCUS       sequence-246774-         234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GB1 mutant with no Methionine residues.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Chen Q, Xu S, Lu X, Boeri MV, Pepelyayeva Y, Diaz EL, Soni SD, 
            Allaire M, Forstner MB, Bahnson BJ, Rozovsky S
  TITLE     (77)Se NMR Probes the Protein Environment of Selenomethionine.
  JOURNAL   J Phys Chem B. 2020 Jan 30;124(4):601-616. doi: 
            10.1021/acs.jpcb.9b07466. Epub 2020 Jan 7.
  PUBMED    31846581
REFERENCE   2  (bases 1 to 234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jpcb.9b07466"; journalName: "J Phys Chem B"; date: 
            "2020-01-30- 30"; volume: "124"; issue: "4"; pages: "601-616"
COMMENT     Sequence Label: His-TEV-GB1 Initial Gly
FEATURES             Location/Qualifiers
     source          1..234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             70..231
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
ORIGIN
        1 atgagccatc accaccacca ccacgactac gacattccga cgacggagaa cttgtatttc
       61 cagggtcagt acaaactgat cctgaatggc aagaccctga agggtgaaac caccaccgaa
      121 gcggtcgatg cagcgacggc tgaaaaagtt ttcaagcaat atgcgaacga taatggtgtt
      181 gacggcgagt ggacttacga tgacgccacg aaaaccttta ccgtgaccga gtaa
//
