LOCUS       sequence-246826-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tagless S19-T48C mutant 30S ribosomal protein in E.coli
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Duss O, Stepanyuk GA, Puglisi JD, Williamson JR
  TITLE     Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA 
            Folding.
  JOURNAL   Cell. 2019 Nov 27;179(6):1357-1369.e16. doi: 
            10.1016/j.cell.2019.10.035. Epub 2019 Nov 21.
  PUBMED    31761533
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.10.035"; journalName: "Cell"; date: 
            "2019-11-27- 27"; volume: "179"; issue: "6"; pages: "1357-1369.e16"
COMMENT     Sequence Label: pRSF-1b-S19-T48C
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(355..373)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      369..416
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttgacggtac attcccctgt agaataattt tgtttaactt taataaggag atataccatg
       61 ccacgttctc tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa
      121 gcggtggaaa gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt
      181 cctaacatga tcggtttgtg catcgctgtc cataatggtc gtcagcacgt tccggtattt
      241 gtaaccgacg aaatggttgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc
      301 ggccacgctg ctgataaaaa agcgaagaag aaataattac ctaggctgct gccaccgctg
      361 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      421 aacctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa
      481 accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg acaagctgac
      541 gaccgggtct ccgcaagtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      601 tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga aaaactcatc
      661 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
      721 aagccgtttc tgtaatgaag gaaaaaactc accgaggcat ttccatagga tggcaagatc
      781 ctggtatcgg tctgctattc cgactcgtcc aacattcaat acaaccttta attttccctc
      841 gtcaaaaata aggtatcagt gagaaatcac cttaatgacg actgaatccg tgaaatggga
      901 aaagttttga tttctttcca actggttcac aggcagcatt acgttttctc aaacactcgc
      961 ataacaaccg gtatcatctg attgccctga ccagacaaat accggtccgg taat
//
