LOCUS       sequence-246827-         516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tagless 30S ribosomal subunit protein S14 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Duss O, Stepanyuk GA, Puglisi JD, Williamson JR
  TITLE     Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA 
            Folding.
  JOURNAL   Cell. 2019 Nov 27;179(6):1357-1369.e16. doi: 
            10.1016/j.cell.2019.10.035. Epub 2019 Nov 21.
  PUBMED    31761533
REFERENCE   2  (bases 1 to 516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.10.035"; journalName: "Cell"; date: 
            "2019-11-27- 27"; volume: "179"; issue: "6"; pages: "1357-1369.e16"
COMMENT     Sequence Label: pRSF-1b-S14
FEATURES             Location/Qualifiers
     source          1..516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(361..379)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      375..422
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta ataaggagat ataccatggc taagcaatca atgaaagcac
       61 gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct gaactgaaag
      121 cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt ctcaagctgc
      181 agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt caaacaggtc
      241 gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt gaagccgcta
      301 tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attaacctag gctgctgcca
      361 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      421 tgctgaaacc tcaggcattt gagaagcaca cggtcacact gcttccggta gtcaataaac
      481 cggtaaacca gcaatagaca taagcggcta tttaac
//
