LOCUS       sequence-246829-       22456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To make transgenic mice for HH5-1 TCRa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22456)
  AUTHORS   Xu M, Pokrovskii M, Ding Y, Yi R, Au C, Harrison OJ, Galan C, 
            Belkaid Y, Bonneau R, Littman DR
  TITLE     c-MAF-dependent regulatory T cells mediate immunological tolerance 
            to a gut pathobiont.
  JOURNAL   Nature. 2018 Feb 15;554(7692):373-377. doi: 10.1038/nature25500. 
            Epub 2018 Feb 7.
  PUBMED    29414937
REFERENCE   2  (bases 1 to 22456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25500"; journalName: "Nature"; date: "2018-02-15- 
            15"; volume: "554"; issue: "7692"; pages: "373-377"
COMMENT     Sequence Label: pTa-HH5-1
FEATURES             Location/Qualifiers
     source          1..22456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16993..17010)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(16993..17009)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17002..17024)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      17450..17963
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(17537..17556)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     17747..17768
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(18445..18464)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     18564..18586
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(18624..18642)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        18710..18814
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(18815..18883,18884..19675)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(19033..19052)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      19846..20434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     20335..20354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     20588..20605
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    20722..20743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(20758..20763,20764..20781,20782..20788)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    20796..20812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     20801..20823
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     20820..20836
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     20820..20836
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccccagcttc cacgcagagg tagagaactg actattgaag ttctctgacc gtcgcagggc
       61 accgcgccac accatccccg ccatgagact gagcctacag tttatgcatc tattatgcta
      121 tgtattccct cagcagggga gccttgtcca cccatgctcc ctggagtgga cagggctcgg
      181 gtttgccatt ctaattcagt cctaaagagg aaccaagtaa gtgcacacaa tcacagctga
      241 gtcactgaag cagaatgtca gaaactggtc tggttagggg ccctgcctag ctgatggctg
      301 cacctagcac taggacttcg tgttcgctct gtttggtggg aaaccaaggc atttggacta
      361 gtccaccgtg tttttttgtt tgtttgtttg ttttttgttt ttgttttttt gtttttcgag
      421 acagggtttc tctgtgtagc cctggctgtc ctggaactca ctcaggagac caggctggcc
      481 tcgaactcag aaatccacct gcctctgtct cctaagtgct gggattaaag gcatgcgcca
      541 ccactgcccg gcccaccgtg gtcttatata agccagttcg tatgtttcct aagttgtcaa
      601 aggggcagag aaatgcgctg ccttggttca ggtttgtagg atcgaaggag gtaatgtttg
      661 ctttgatgtt gagcaagcac acactatcgt gactcgggat tctagacgag gatgttgccc
      721 gctcatgtga tatcattgga ataactcagc cagggtcagc aaggagaagg aaggatgccc
      781 tagagtcgat aaaaatcatg tcttgcgcca ggcatggtgg cgcatgcctt taatcccagc
      841 acttgggagg cagaggcagg tggatttctg agttcaaggc cagtctggtc tacagagtga
      901 gttccaggac agccagggct acacagagaa accctgtctc gaaaagccaa aaaaaaaaaa
      961 aaaaaaaaat cgcgtcttgc agttttgttg accacagtct tttaggttta ccttgggtat
     1021 ttcctgccca gactgaccat gaaacagacc agttgctccc acaacctaaa ccatgttatt
     1081 tgatccactt cactctcaag ttctgccagt cccatcttac gtctgtctgt gcttcttcca
     1141 tctggtgttt tctttttttt tccttggttg ataaatttaa tgtggatgtt tcagagaatg
     1201 gaaggaactg gatggtttca aagtggattt caggagggct tcaaaactgg aatcctgtgg
     1261 cctctctttt tgtttttgtt tatttctgtt tatttgttta ttttggagac agggtttttc
     1321 tgtgtagccc tggctgttct ggaactcact ctgtagacta ggctggcctt gaactcagaa
     1381 atcagactgc ctctgcctcc caagtactgg gattaaaggc gtgcgccacc actgcccagc
     1441 cctgtggact ctggaagggt ggtatgctca catttccagg acaaacacac cctaggatat
     1501 tgtaggacac actggctgga ggaggaatgc ttctcagaaa acctgccttg aggggaggtg
     1561 cctggagaga ttgtatcctg ctactactgg ctcaaggaat cctacaatca gggaagcccc
     1621 tggggtgcca gagaggaggg aaaaaaatgt gtctcctcac aggtgataca gtgagaagca
     1681 tcaggggggc catgggatac cgtggggtcc tgtggtcctg aaaaaactgt gaccttgcaa
     1741 aagctggaag tcatggaagg agtaacagat gcgcgtgata gaagctactg gggccacagt
     1801 cacgggcgag gtgggggacc gctgctgagc gggggacctg gcgggcccca agcctatgtg
     1861 agcgccggca tcctcacgca gcaggctgct tttatcgcag catgggggtg catgggtgca
     1921 tccgagcaag cgcgcaggat tatggtccat cctttcgctc gtgctgccat cccactgctt
     1981 tgtgtctgcc tggggatttc ggttcgcgaa gcattagcat accattatct cctcagacaa
     2041 cagggagaga aggtgggcat gcattccggt ctccgtgtga ataatggaaa atggagagag
     2101 acaggggaaa gttaaatgtg ccacgaagga cactgaagct tacagaccct gctttgcctg
     2161 acccctgcct gcctgaggac tgccaggaag atattaacag aggccataaa attacagcca
     2221 ttacgtctgt ctcccatgct cacacagtgc ccggactgtc tgtgccttga ctttaggcaa
     2281 tcttgagttt aatttacaca cactgaggtg cagcaaaccc aggctttcca gctggatgca
     2341 ttttgacagg catctacccc gatgtgccca tcatccaggt tcagatcaac cgccccgaat
     2401 tccaaaccct tgttctcctt tcccatcagt cctttctccc tagagacacc tagcatgtga
     2461 gtacctgtta tgggcgaccc ctttggccat ttcttaaact ttagttgtat ggagtcagag
     2521 tccctgaaac ctgacacagt gtctgccctt taacaggtat cccgctggaa gaaggccttc
     2581 gtttctaaac tacctgagag aatgttggta caaagaggag aactgaggct gcgggtttac
     2641 aggctacaga gtgaagtcat ggatcctggg cctgccgggc cccatattcc atctcctggc
     2701 ttccaacagg gtagtttctt tctgggtttc tcttgtagct gggtctccta aacctgggca
     2761 ggcagtgccc cctgctgcct gaaggcctga ccctagactc tttgcaggca gagaggatca
     2821 cacaagtctg gagtccagtg actggcacag ggcaggaccc tgtgaaacag aagccacttg
     2881 tcattttatt ttcctttgga aagagttttt ccgtaggatg cagggatttt ctttatcccg
     2941 aataaacacg ccgagggaga aagccttttg gtggagtact gctgcccccg tgtggctacc
     3001 ttaagacact acaacagagt gggactccca agacgaaagt ttttcaggtt tcaggttctg
     3061 cgacttccta ggaccctgag agacagatgt ccactagggt tatctcttga cacttaaatt
     3121 tcctcctgga gtggaaatga gagcgaatgg ggcttcttgt ggaaggtatc tgttggggaa
     3181 cgagctttag ctgtgtgtgt aggcagctgc ctctttcaca acctgtccag cacaagctgg
     3241 actggacata gactttctcc cttgaactaa gtatatagac ttgtctaatt tgagggcaat
     3301 agaggtcagg ggagggggac ttcttgggag tggagccgca gtgcctcccc agccggctgc
     3361 tgtgaatggg tgttaccacc aagacctgca agccccaccc aatgtgctct ccgtggcctt
     3421 cttttctgca cctgtggttg ggctgcggtg taacggccca gcctacctct tctgggcacg
     3481 tggcccggag agatcttatc tctaactctg tatttttaac tcctctttcc agaggatgtg
     3541 gcttctgcgg gagagcttca aagggggacc tgtttgccca tgtcagcaac ctgacgtcat
     3601 ggaagtggga ggctgttcag acccaaacac ctggagggaa gtgggaaact ttttccattt
     3661 cctgttctac ttctggctat tgattcacta tctgaccagc ttacccattt cagggtgcag
     3721 ctgtctgtgt gattcttgga ggaaatccta tgccttgtgt gtctgtatga aaggagacag
     3781 ctgtgttacc catgtggctt ttacccaaag cagggctggc gatccttcag actgcccccc
     3841 accccacccc acccccaccc gtagccagta catgcaagag ttacagtatt tgacaggtct
     3901 ctcacactca cctctgggaa atatccctcg gggacccaga cttcaatctt gtccagggcg
     3961 gggtgggctg gatatagact tcctgctttg taaaccggaa gcccttcttc ccacatgttg
     4021 ctcgaggaac taggtttcag tgggtttgtg tgggtcactg gggaaactga aatggacatt
     4081 aagtgacctt tggccctgga tgctggttct catgtaggac cagagtcttt agacttttag
     4141 aagccagtgt ttcctggtaa ctgtcctgca ggattcagtc aacagtcatg aggctaatgt
     4201 gagtctgaac tcttcaaggg cgcaccactc aagctctgat tattctaata gtttttgcca
     4261 agctactcta ttccttgcaa aaccgagaca gtggctcacc tggcctctgt taacgccacc
     4321 acattccctc tccagctcag gtccctccca gtgactgtgt tgaccgtcat ggggtcatct
     4381 gttggtatct gttgggtgca aggtactgca ctaggtatgg aggagctact ggtgagcaag
     4441 gtattcctcc tatccgaagg gtcatctata gtcccttgcc aacatgtcac ttggtaataa
     4501 aacaatatta gatttgatat agacatgagc tttgggagtt ggattttatg gtatacccca
     4561 aacaaaaatg agacaaaact aaacccttga agcctcaggg ggcggcctga gtggtcagag
     4621 cacacacctg tccagcctgc ctgtttctgg actgaactgt gactccagct gcactggaca
     4681 acgttgcaga acactttcca cagttctagc cttggaacgt gaagggtcca tttttttttc
     4741 tgccttcaga gctggcctgc agaaagccct tgagggagta agggcattta tctagactct
     4801 agagataccc attttacagc tggccctggc aaggagtttg ctggggtatg ggtgccttct
     4861 ctttgggagt tggtccagac tggtctggga gcagtggcag ccttgtgacc attgtcatgt
     4921 cttcaggttt cctcagtagc ctcttccaga ctaagcagtg agagatccag agtccgacgt
     4981 ggaagagtct ctgcctgttc cccacacttg ttcagctcct gtggagattt agcctcagct
     5041 gtgggagaaa gttcttgttc tgattggatg gcgatgaagt tgactttgat ctatcagtgg
     5101 cctcctgtct gccttgtcca ggcctcgctt gagttggggc tggaagctat ctatattcac
     5161 ttcaccgctg tgggaggccc ttcaaatccc tcccacgtca cctcactctg gtcctcaggt
     5221 gacgttaggg ttcttgtgac ctctggatgt tgctctctgg ggacactggg ctagcatggt
     5281 tcaaccgagg ctgggcctct ttcacgttta gtgaggaggt ctgtctttga ttcctatact
     5341 accttctagg cttcttactc tgtcccattc ccacaaggtc ccccttcagc ctctgcatta
     5401 tggcagccag tttttaggag cccggcatct cctcagccac ccacgctttc tttgcctgga
     5461 tttttttttt ttttttaata gaacaaagtg aattaccact ttaggagcaa ggaaaaaaaa
     5521 aagacaaact aatttgccct aagttcttaa tggttgataa aggcttgctg cactaacctt
     5581 cccatgggca ctttcatcta atacagggaa gagaatggtt taattaaaag aaatccccag
     5641 tggtagtctt tggcatcgtg tcagacactc gaggaggggc gtgggattga ggctcttcgc
     5701 ccctcctccc ctgtgtgtga gggtggccct gtcatggtgg tgtttcctga ggccgtcccc
     5761 gtcactggtc tcagaaggct tcattgtggg ctagagatga ggcagtttag aaatgacggg
     5821 gttctgcggt cactctaatc aggggtggcc gggagaggga acatgggttt ttgctgtagt
     5881 ctccagtcga aactgacatt tcatggctgg tgaccctgaa gctcacagga gggagggaca
     5941 tgccctcgat cacactcgaa agctggtcag ccccatctct atgagaagaa gcaggcctgg
     6001 gagtcacagc tgggaggatt agcatatttc tggctagtgt ggtcatagac acacagttga
     6061 accagggaaa agctgggtgg tggcagtggg tgggggtggg ggagtcctgg gaactagtaa
     6121 gtactcccgt ttggtgtgag cagttggtct ctgaagtgtg tttccatcag aatccctgcc
     6181 tgcccttggg gaaaggagac tgataggaga ggctccctct ttgctgtggg ctgtgaaggc
     6241 agagactctc atggctagga agagctgaga atgcctgctg tccttggcca agtcccagac
     6301 agtagatttg taaggcagaa cacaggttca gtagcaaggc agtctgatgg gagagcaagg
     6361 gtgtccctgg agggcaggag ccagtgggtg agaacctgga ggacattcct catccttgac
     6421 tggtaaactt cagctacagg tcagagcctg cccatcagcc ccctgcggtc agcctcttaa
     6481 tgggtgcggc atgagcgggg actcacccct gtaaaaggag cttcctggcc cttggtacag
     6541 cttgcttagt ggaatccgcc cccacacccc ccaagacagg gtttctctgt gtagccctgg
     6601 ctgacctgga actcactctg tagaccaggc tggcctggaa ctcagaaatc cgcctgcctc
     6661 tgccttccaa gtgctgggat taaaggagtg cgccatcacg cctggccgct tagtggaatt
     6721 tttaagaggt cattccaaga ggttgaatgg agagggtttg ttgaggtaac tgggcaagtg
     6781 tgataggcac atacttcggt ttctctctgc atgtaaatgc ccccaccaag acccagtcaa
     6841 ataggatcac agttttggtt tttctgaatc acctttaatg atgtcatggg cagcagaata
     6901 atatacaatg tggctcaata cacacagtag caggagggtc tagacaatga gataatttct
     6961 acaccttgat gaaagagtaa tatgcatttc tttatggatg cttaaaaaag taaacccgcc
     7021 aaaaaaaaaa aaacccatat tttttacaac attctccaag agattttata ggggagtctg
     7081 ccatggggga ggggtcttca gatccaggag aagccccgcc cctgccgttt tttttttttg
     7141 tgaatggtca gcagcagtga gggagctgag tgggtgcggc agcagcgctt agctctctag
     7201 caaccttcct cacaaatctg ggagatccag gtgggattgt gaatcagggc caaccagacc
     7261 ccagacagcc ggctcctttt ggcttgaaga gggagcggag gggtgaggag gacggaactt
     7321 gggagtcagg ctctgtcagt cttgcagacc tggagtgagg aagtgcagag gtcaggcttg
     7381 gcatggagga ctaggacccc caggggtgtg atgaggatga atttcttagg ggctgtgcaa
     7441 ggtgaccatg gaagcatgat gactctagcc aggtagaggg ctaatgagta tagcatcatt
     7501 gaatatcaag cagagagagc ttgctaggga gaaggcacac aggaagggag tgtacactcc
     7561 agaaggcagg gccagagaca cgtggtacgt gtgcatcagg gtccggagtt ttctcctcct
     7621 ctgttctttg gctgtgtgag ccttgatgtg acttggcctc acttccatca tttctaaatg
     7681 agcatgacct gagtatctta ggagagtcac tgctggacca aggtcgctga aaggattgtg
     7741 ttctgcagcc caggtgtttc agggcactgt tgcaacagtg ttacaacttg taatgggtag
     7801 ggggtagaaa agttctgtgg gtccagtctt aggatttaaa agcaaatttt aaatcccagg
     7861 gcgatttgcg ctcagggttg ggtcttgccc tgaatccaca cctctaagcc ttctccaggc
     7921 tttcatgcct tcttacctca actggaccac agcctcagcg tcatgagcag gttaaatccc
     7981 gctactttca gcaggaggat tcggagtccc ataactgaca ggttttgaaa gtttaggttc
     8041 atatctgtaa agccaagagg tggtagccat taacggtatg tgcatgagag ggcagtacag
     8101 caactcacga cactgtggac tttcccaaga gctggttagg catgcttggg tgtgggtaac
     8161 ttcaactccc aggttgcttc tttgagcttc tgcacacacc ccaaggcaaa ggtatttgta
     8221 tatccctcca gggtaagagc attgtgtttt aagacaaaaa ccaccaaaag gacttagtcc
     8281 atataatatt ctgaggccct gtacggatgg gggtgggggt atgggtgtgg gtcggggggg
     8341 ggggtagatg ttcagaactt gtcataatgg tctagacctg ggcctctcag tgaaaccaca
     8401 gatgtgatga ctcagtaact agtcacgtgg aatcgaggtg gcattgagac aagacctttg
     8461 cctgaggcta tgtgagttct atttcctttc tagaaaggaa ttcccctgag atacagagag
     8521 ggccagtgtc catagagtga caaggttagt tcaaggctgc tctgaaggga ctcacaagtt
     8581 ccacagtagc atatgaagtg gttttctttg gctattctca cagaggagcc aatacaagag
     8641 acaccaaggc ccgagagtga ctcactggcc ttgtgaaact ggaggcaccg cttgttaagt
     8701 acttagaatg gaaaatgacg ttaaccgtgc tggggattca agacagaccc tgcgaagctt
     8761 ggggcaggac tggaaatgct gttcccaccc ccagatccac cccctcccct cccctgtgca
     8821 ctaaaggtca ttactggccc ctccccaccc cccagcatca ctcagtgttg gtcagaccct
     8881 tctcttacct gtttcaaagc ttttctcggt caacgtggca tcacagggaa cgtctaagag
     8941 ggaaaacaaa gacatttcct tagattctga agcgtgttaa ctgtcccacc acaggttgtt
     9001 ggtcaggaaa atatatcaca gcttttctgt gatcctttgg gagagagctg ggggccatat
     9061 tgcctaaggg tatctgctgg agcaaaggcc caacaccctg gcttatgggc actcttttat
     9121 tctttcaaca actatgggct gtgcaaagta cgtgctgtat gacatgggcc aaaaagtcaa
     9181 gatagtccct gaacctggag gtttcagtct agagtcccgt aaagacagca agacatattc
     9241 acagtgacca gggccatgta ttttcatgta cacctccccc caatagtgtt gatacacaaa
     9301 tttgctcctt tattatctct tacttagtat aaggtcccat cgaccgagat gctctgccac
     9361 ttcccttgat gcccattgac cgatctctgt gtggttctat ctgtgaaacc taagtgctgt
     9421 tggctgctgg agtcctgcca tcacctggtg gggcttcctt caactttcta tgttctctgt
     9481 cttctgctgg atctacagtc tgccttctac acaccactca atcttatgtt tatttagttg
     9541 ttttgggaga cagggtctcg ggtagtccag gctggccttg aactttctgt gtaggtgatg
     9601 atgactttga acgtctgctt ctcttgcctc tacctgtgat ctgacccccc tgtctatggc
     9661 ttacaatcca tttcctgcag cttctcctta ttctccggat tctcctttct tggcttctga
     9721 caggctcctc tctggcccca ggcagggctg gtcccttctt atctattaac ttgtagtgtg
     9781 atagcatttt ctctgagcct ttctctaagc tgttcttgcc ttaactctga gttattgcat
     9841 atattaattg cagcctggtg tcatttacat gtccctctgc tgacggatgg tcctcctgga
     9901 ttagagtggc gtcccctcct cctcatctga cttctgtact cacactactt ggcagacata
     9961 gaaatcaggg caggcggctg ggggtgggtg ggtgtgggat gggcaggaag ggaatggaaa
    10021 ctgtgcctgg gagaacagag aaggcacttg gctcaagaga agacaggaag gtgagcctca
    10081 gaattgtgag accaacgctg ggccaatgtc ctctgtcatg ttctcttgtc tgctttggtg
    10141 acccgttagg actccttagt gacacaggat gtggattttg ctgcctaatg atgagggtga
    10201 gatgacccaa agcagctttt ggaaaccata gatttgagcc aggaggagac ttctggggca
    10261 ccactcacct gaactggggt aggtggcgtt ggtctctttg aagatatctt ggcaggtgaa
    10321 gcttgtctgg ttgctccagg caatggcccc attgctcttg gaatccatag ctttcatgtc
    10381 cagcacagtt ttgtcagtga tgaacgttcc agattccatg gttttcggca cattgatttg
    10441 ggagtcaaag tcggtgaaca ggcagagggt gctgtcctga gaccgaggat cttttaactg
    10501 gtacacagca ggttctgggt tctggatgtc tgtgggccaa gaggactgat gttagaagat
    10561 tttttggggg tcctaactta ggtcagaact cagatgccag tgacaggcaa gagcggaact
    10621 ctctggacta gccagtcttg ttcacagact tcacattgga aatgacagcc taggatctag
    10681 ctgcccatga tggacacagg cttagggaca ggcacaagtc atgagtgcca gccaagatct
    10741 ctagctttca gtagtccctc tccacggggt cctttctggt atctgggaaa accagaaagg
    10801 aaggtggaac cactaaacca tcttgtcatt tttctactta acaggatctt cagaacccca
    10861 gtgggtccag tcaaacaatt ttgttttggc aggacaaaaa caagctcaga aaacagagac
    10921 ttagatgtct tctctagagt actctccccc aggtaggctg gacgttggtg agaaacgaag
    10981 gcgtgtgata ttttaacatt aagttggaaa atctctcaca ctaccgggcc atttttcttc
    11041 tctcttgata aatgttttga atagcactca caccagagcc ctgtctctac aatttactcc
    11101 ggtcagcctg gattaaatca acaggcagct gaagtcgcag aattttcacc tgtaagaggg
    11161 acaccaggct gttctgagaa atgactattt cttcaaataa aaataaccga gtaaaccaca
    11221 caccgggcac tgaacaaacg tgcccggctt tcaccatgaa aacataccac ttagcaaacc
    11281 aatcacactg tctctttatt ccaggaaagc gggtgctacc tgaagacaat tctttccttt
    11341 ctccaaggga ttttgttttt aatgtgactc tctcagacta tttccccatg ccctccccgg
    11401 tctgaggagg ctggctggag agattaacac agcggaccac ctagagagtt tccagcaggg
    11461 ctgtgatcca aagctccttc ccctgtgggt ctcccacagt tcttaactga tcacaggagc
    11521 caaccagggt ggccttggca atgctgactc ccagctggat cctaaaccaa aggaatcagg
    11581 ccctggtgcc attgctcaag gatgctgagt taagacaaga gctgtagcaa gcaaggtact
    11641 gcctcggagc agcagagctg gggggggggg gtgccgtggt gggacatgga gttctggccc
    11701 ctttcaggca aatatttaag gtctcaccac agagacgtcc tggtgtttag tccccttcat
    11761 gtccagcccc atagatatct actgcaaccc cacctttacc tcctgaaaat gagaaacaaa
    11821 tcccctttaa ctagaggtct tcagggttcc tgtctctcag agccctgcag cgagagacat
    11881 tctatttcta cctggtcaga aaaacacatt tttaccactg atggcatccg aggtcacttt
    11941 agcaagactg gatcaatttc tcagctacag gttcagccgc tctgattcct aacttgccat
    12001 cataattcaa aacatacaga ggcggcgcgt gtctgagtgt ataagcgagg taggtcccca
    12061 ggggcattgc agtgatggag agagtccctc accactaaat ctgtgctctg ttgttaagta
    12121 ccggaaacct cggaatgaga gttagtgaca cagatgtggg tcctactcag gcttgggtag
    12181 cgtcaggaaa acagcaggca ggcttgtcag gaaagatgga gcagttatct gcacaccagt
    12241 cctaatgctt ccttacagta acaccccctg attgtcttcc aaggaggctg ggatgaactc
    12301 acctaggagt gtgatgaaga cgaagggggc gggggggggg gaattcctga cacccctcac
    12361 ctcacatcta gtcttgctta ctaaactgga ttctcagttt tcttgtggct ggccctggtc
    12421 ccaatctctg tattccctgg gacgttctag tattttagta tttcttattc ccactgtcta
    12481 agaaggttcc tttttttttt ttttccttgt tctcttagtc tctttttact atcagatctg
    12541 cctacacaag ccaaaaggcc tgtgtcttgt ttatcccaag atcaaatctt ccttcttgtt
    12601 gccccttggt tggaatcctg cctccctcct ctctctgact tacacagatc ttatcaacct
    12661 gtcaaggctc aacaaatctt ttctagtaca gaaagcagcc tcagtctagg gcaccttctc
    12721 caggaccctg accttctgag gctgtttatc tacaccagca gttgccaccc cttaaatagt
    12781 ttgtaggttg ttaaatgtac agtagttctg aacatttgtc tagtttctat gctgtgtgcg
    12841 gacaggtcag acatctatct gtcttgggct tttttattct cagcctaaca ttacctagag
    12901 tgatagctac ttagaggcat ttaaaagtgt ccattgaatg attaaagtca tcttttaatg
    12961 agtttgacct agagaaaaca cagatattta agattgatag tgatgcctcc ccccttcaca
    13021 gacctccgta aaacaattag caggataccc cagttagcct caccctgcca tgggaacagc
    13081 ggacatctct ctccagagag agggttaatg accgtgccct accgggttgc aaatggtgcc
    13141 acttggggta actcaccaga tattaccgtc acttgggtcc cttccccgaa tgttaattta
    13201 ccacttagtc ctccagtatt cacactgcag gtgacacctt tacagaagcc taggcgacct
    13261 acctactcct tagatagaaa gaaagtctct cagggtggag aatcccaggc ctgaaactga
    13321 gcccctttgc tgtcttgcat tggaattctg tctctgctgt agacagtcct ttcccctgcc
    13381 ttgttctact gggaggctcc acattgaagt aagcgaagtg atttccacaa attcaggaga
    13441 atttatgaga tcaaaacttc ctgctcagag cagcagtttc tcggatactc taatcgaagg
    13501 atctagatta tcaaccgcgt tatgattgcc aagggtccag agcaaggaca atgtttcttc
    13561 aggacttagt gctgggtggg atgggttctt tctagaaaca tttgcaatcc tacagaagaa
    13621 gataccagaa ctccagccag tgtatcttgg acagagaggc agaggaagtt cttgtaaatc
    13681 ctatccttga ctcattctac gtatatggtg gatttctgag tttggtttcg gctccaaaga
    13741 ttagtttaga gctgtagctg aattcccagt ccagtccagt ccagtccagt ccagtccagg
    13801 tctttctgac accagtcaga gcccactggg gtctcaggat atttccctaa actgtcctgg
    13861 agttcttggt cccttacaac ttctatttac caaggaagct gttgtttcgc tctcttgaca
    13921 acagtataaa cctcgtgtgg caaggccatg atcagatcat agtaccgagg atgcattaca
    13981 ctctcccaat ggaggagttt tgtttgttgt tgttgtgtgc aggcaacgtc tcacatgata
    14041 ccagatgatg tggggagatc actttgttgt tgaaagtgga aacactgtta aatatagccg
    14101 gtgaattaaa cgctattaaa tagagccaga attcagcaca ggcctatcct ccttcntcct
    14161 cctcctcctc ctcctcctcc tccatctccc tccctcccta tctatcttca caaaacagag
    14221 tacactggcc agtcacggag ctccaccgcg gtggcggccg catttgattg atttcccccc
    14281 aaaggcccag gctaagaaga gggtaactta cttggcttca ctgtgagcac ggtcccatcc
    14341 ccaaaggtta atttgcctgt attggtatca tcagtagcac agaagtacac agcggtgtct
    14401 tcacaccgag tagcagtgat ggacaaggag ctgctctggc tggaggtgtc aagggtggct
    14461 ctgagtcttc cattgcgctt ctctctctca tttgaacgta ttaagattag ctgggcaggg
    14521 cctttgcctg acttctgtct gtaccactgt agggcagtta tggatgtctt gtaactacaa
    14581 ttcaccgtga cactttcacc ctcgtggacg ctcagggccc acgaattttc ttctgctagt
    14641 tgactattca cccttgtcag ttgcagccac aacaacaacc ccaggaggct gtgcatggtg
    14701 gcagtgagaa gcccgggaga tgtattcagg aaaatctccc tgtggacaag gcctggagac
    14761 tattaactga gagatgctcc tcccttggca atgactgctc tgtcgtcctt tctacttaca
    14821 actgcacttc tgagagctgc aaggtataaa ggctagctga agtgacagga tttttttttt
    14881 tctttcttgc ccttatagac attcagtcta tgcaacagtg cccccttgtg gtcaaaggtg
    14941 gaaaaattag gccttctttg gtgttaggtt cattgaagca ataaaatctg gtgaaagtga
    15001 atcttaagca gatgatctaa ttttatgagt taagtagtac tatttaatct tatgagctag
    15061 aaaatgctac cgtcctcgtt ttataaacta gaaagcttaa gaatggaaga aagtgtgtgt
    15121 cacagcaagt gacaagcctg gagtttctct cattactatg gccaggaagg cccatcagta
    15181 atgactaatg actatgcaca tttatcacaa cagcctttta cagtgttagc tgtcatatat
    15241 aatgaacagg aaatcaaaac tttatagagc taactaggtt tcataaggtc acagaccacc
    15301 agcttgtctg ctcctctgaa gacccaacag tctataggcc tcccaggaaa tctgcggcac
    15361 ttttattttt tattgcacat cttattagtg aaggaaaaag aaagaagtgc aaacagtgac
    15421 gctagccttg tggagtgagt gggagaaccg ctctgcacag gaggaggaac gactgatagg
    15481 gccaacaaag gaggtacaat cagtgatggc catggaagtc ctaagtaaat tctacttaag
    15541 cagcggttgg gtggggaggt cctgacacat gtccccaaac aaagatctct gggaggacag
    15601 aaccactagc accagggtta tggaggagca cagaaccaca aacccgagaa tcaaggcaaa
    15661 actgtcagct aaagccttgc ttctgtagtc agagtctcct tagggaacag tgacagtcag
    15721 aggatgcgag ccaccgctgc ctttagttaa aagatgccct gatggttgct tagaaagtaa
    15781 taaagaaaga acaaaataat tattgaagga ccataaaagc atcaggcaag cctgtttaga
    15841 ttagaggaca gtccagctag actctaaaga tactgatttt ttttttctca aaagtgattt
    15901 ttttttttgg caggtgattt ggggaaatgg aatgatttct ttccctgaat gtagaagcaa
    15961 ctctttgacc taccagtacc atttgcaaat ctgtttagaa ctcagcctgg catagcagta
    16021 ccccataatc ctgctaccag cttgtcttag ttaggatttc tattgcttca gtgaaacatg
    16081 gaggccagaa agcaaggagg ggagggaaga gttagtgtgg tccaatcaag gaaggaagac
    16141 agaactggaa tgcaaaccgg acaggaacct ggaggcagga gctgatgcag agtccaagga
    16201 ggggctctac ttattgcctt gctctccatg gcttgatcag tctctttctt ataaaaccca
    16261 gatctgccat cccagggaat ggaacgaccc acaataggct gggcccttcc ccatcaatca
    16321 ctaatgaaga aaatgcccta tagtctaact gcagcccatt ctagcacgga ggagcttccc
    16381 cgacccccgc cgtgttgttt gacctctatg gtgaatctat tcttgtgttc taattttaac
    16441 agcaacaatc ataaattccg aagtatcaca taattgaaca agtcaggaaa tctttgggca
    16501 tctgtttttc tccatcagtt actcttgctc ccgtatctcg ggttgttttc taaacacaaa
    16561 aagtgaagag cgatggacat aagttagcaa caaagaaggg ggagggaggg agggaaggag
    16621 ggagtaatgg aaggagagag aagggtggga gagagagaga aggggcagag agggagaaaa
    16681 caagctcaga catcctctct gccgtgtgta gtcatgccga gacttatcta caagacatgc
    16741 cattacttcc agcctccact aacccatata acagggcctt atgtcatcag cctgccagtc
    16801 cttctgagcc ctcctacatt attacagcag gggaaggctg catttccccc agacagttcc
    16861 tactacacca cgttcaaacc agtttaagct atgaacacta gagggcagca aagccagcgt
    16921 taagtcacaa aagttagttg gccatcaggt cggggatcct ctagagtcga cctgcaggca
    16981 tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
    17041 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    17101 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gattccgttg
    17161 caatggctgg cggtaatatt gttctggata ttaccagcaa ggccgatagt ttgagttctt
    17221 ctactcaggc aagtgatgtt attactaatc aaagaagtat tgcgacaacg gttaatttgc
    17281 gtgatggaca gactctttta ctcggtggcc tcactgatta taaaaacact tctcaggatt
    17341 ctggcgtacc gttcctgtct aaaatccctt taatcggcct cctgtttagc tcccgctctg
    17401 attctaacga ggaaagcacg ttatacgtgc tcgtcaaagc aaccatagta cgcgccctgt
    17461 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    17521 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    17581 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    17641 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    17701 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    17761 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    17821 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    17881 aacaaaatat taacgcttac aatttaaata tttgcttata caatcttcct gtttttgggg
    17941 cttttctgat tatcaaccgg ggtacatatg attgacatgc tagttttacg attaccgttc
    18001 atcgattctc ttgtttgctc cagactctca ggcaatgacc tgatagcctt tgtagagacc
    18061 tctcaaaaat agctaccctc tccggcatga atttatcagc tagaacggtt gaatatcata
    18121 ttgatggtga tttgactgtc tccggccttt ctcacccgtt tgaatcttta cctacacatt
    18181 actcaggcat tgcatttaaa atatatgagg gttctaaaaa tttttatcct tgcgttgaaa
    18241 taaaggcttc tcccgcaaaa gtattacagg gtcataatgt ttttggtaca accgatttag
    18301 ctttatgctc tgaggcttta ttgcttaatt ttgctaattc tttgccttgc ctgtatgatt
    18361 tattggatgt tggaatcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
    18421 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
    18481 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
    18541 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
    18601 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
    18661 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    18721 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    18781 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    18841 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    18901 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    18961 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    19021 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    19081 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    19141 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    19201 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    19261 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    19321 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    19381 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    19441 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    19501 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    19561 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    19621 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    19681 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    19741 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    19801 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    19861 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    19921 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    19981 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    20041 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    20101 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    20161 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    20221 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    20281 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    20341 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    20401 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    20461 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    20521 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    20581 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
    20641 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
    20701 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
    20761 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
    20821 aggaaacagc tatgaccatg attacgaatt ctgccttcaa agtcaggtga ccccccaacg
    20881 accccatgcc tcacagttgt aaaaagcaaa caaacccaga tctctgcttt ggctctgtgg
    20941 atctgctgtt tgacgtctgg tctcatgggc aacgtcttag ctttctttca cattggaaaa
    21001 gaaaaaaggt ggaaggagac tctttaaatg ttaagggatg gatgaaaaac cctaaaaact
    21061 cctttgcttg catctcctga aaatggccaa gaagggggag gtctattgca tcccattctg
    21121 ggctccaaat gctatattag agaagacaac atgagaacaa ttcagacatg gacttacttg
    21181 gaacgacttg gagttttgtc ccagttccaa acctctggta agtcccagaa tttgcactgt
    21241 aagtgaggtc tttacaaaat ggcttctttc ccttcctgtt tccatcagag taactctcaa
    21301 ctcatacact tccatgagag agagagggtg tttttaacca gtgcctgtag ccacagatcc
    21361 acatgaacac acaccatctc ttcatcttaa aagagtctat gactgaccca gctttaatat
    21421 ttttttgttg ttttatagac ccatatttta tatgtggata gcacttcaga atctgtaaaa
    21481 tatttttaca tacattaact tatccttata ttaacttatg aagtattatt aaacactttc
    21541 tttttacaga tagagctggg ccagtgcacg agtcaaagca gatttagggg acctggatgt
    21601 gtttcagctg agctgccctt tcttcaggat cagctctgcg ggagctatta tttctagggt
    21661 tcctacatga cctgtcttgt tcacagccag ttctgcaccc ttattttaac agtttcccca
    21721 tacaaggaat gcgcttgtct gcatgaacca ttaattcttt gaccactgtg cctcacaaat
    21781 actgtggcaa gttcagtggg ccttagcatc cctattaagc atctctttag tgatgtcttg
    21841 tcccccaaat gaatacattg taaatcgtgg tcccgtgctg tctgacaaaa atgctcaacc
    21901 tgctgtgatc ttagattgat gtgatcagca ttcagctctc ttctctcttg ggagttctgc
    21961 tagggattcc ccctttttga ttactggaat aaaggctaag ccatggaatt aagcccgtga
    22021 aagcctcttt acaaggctca acaacagtgg tgccatagct gtagctaaag cctgagagga
    22081 tagcagctga tattctccag tcaattctga aacatactgg tttttaaaaa gatttgtaca
    22141 atttcaccaa gtcgacggga tccacggtcc tctgcctctg acagggtgtg gttttctttc
    22201 tgtatgcgtg cgtgcgcgtt tgtgagtgtg tcagaggaca tcttggcagg agtcggttct
    22261 ttccttgtac catgtaggta ctaaggttca agcttgggtc accagggcgt cactaggtgc
    22321 ctttgtatac agagtcatct tgtcttctga ttttgcctta tcactgaact tctgacccac
    22381 ggggctggca aggtggctca gagagtaaaa accccttctg cgcagccccg gtggtctgag
    22441 attgaccccc cccgcc
//
