LOCUS       sequence-246830-       22470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To make transgenic mice for HH7-2 TCRa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22470)
  AUTHORS   Xu M, Pokrovskii M, Ding Y, Yi R, Au C, Harrison OJ, Galan C, 
            Belkaid Y, Bonneau R, Littman DR
  TITLE     c-MAF-dependent regulatory T cells mediate immunological tolerance 
            to a gut pathobiont.
  JOURNAL   Nature. 2018 Feb 15;554(7692):373-377. doi: 10.1038/nature25500. 
            Epub 2018 Feb 7.
  PUBMED    29414937
REFERENCE   2  (bases 1 to 22470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25500"; journalName: "Nature"; date: "2018-02-15- 
            15"; volume: "554"; issue: "7692"; pages: "373-377"
COMMENT     Sequence Label: pTa-HH7-2
FEATURES             Location/Qualifiers
     source          1..22470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16995..17012)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(16995..17011)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17004..17026)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      17452..17965
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(17539..17558)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     17749..17770
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(18447..18466)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     18566..18588
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(18626..18644)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        18712..18816
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(18817..18885,18886..19677)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(19035..19054)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      19848..20436
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     20337..20356
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     20590..20607
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    20724..20745
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(20760..20765,20766..20783,20784..20790)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    20798..20814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     20803..20825
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     20822..20838
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     20822..20838
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cgcagaggta gagaactgac tattgaagtt ctctgaccgt cgcagggcac cgcgccacac
       61 catccccgcc atgagactga gcctacagtt tatgcatcta ttatgctatg tattccctca
      121 gcaggggagc cttgtccacc catgctccct ggagtggaca gggctcgggt ttgccattct
      181 aattcagtcc taaagaggaa ccaagtaagt gcacacaatc acagctgagt cactgaagca
      241 gaatgtcaga aactggtctg gttaggggcc ctgcctagct gatggctgca cctagcacta
      301 ggacttcgtg ttcgctctgt ttggtgggaa accaaggcat ttggactagt ccaccgtgtt
      361 tttttgtttg tttgtttgtt ttttgttttt gtttttttgt ttttcgagac agggtttctc
      421 tgtgtagccc tggctgtcct ggaactcact caggagacca ggctggcctc gaactcagaa
      481 atccacctgc ctctgtctcc taagtgctgg gattaaaggc atgcgccacc actgcccggc
      541 ccaccgtggt cttatataag ccagttcgta tgtttcctaa gttgtcaaag gggcagagaa
      601 atgcgctgcc ttggttcagg tttgtaggat cgaaggaggt aatgtttgct ttgatgttga
      661 gcaagcacac actatcgtga ctcgggattc tagacgagga tgttgcccgc tcatgtgata
      721 tcattggaat aactcagcca gggtcagcaa ggagaaggaa ggatgcccta gagtcgataa
      781 aaatcatgtc ttgcgccagg catggtggcg catgccttta atcccagcac ttgggaggca
      841 gaggcaggtg gatttctgag ttcaaggcca gtctggtcta cagagtgagt tccaggacag
      901 ccagggctac acagagaaac cctgtctcga aaagccaaaa aaaaaaaaaa aaaaaaatcg
      961 cgtcttgcag ttttgttgac cacagtcttt taggtttacc ttgggtattt cctgcccaga
     1021 ctgaccatga aacagaccag ttgctcccac aacctaaacc atgttatttg atccacttca
     1081 ctctcaagtt ctgccagtcc catcttacgt ctgtctgtgc ttcttccatc tggtgttttc
     1141 tttttttttc cttggttgat aaatttaatg tggatgtttc agagaatgga aggaactgga
     1201 tggtttcaaa gtggatttca ggagggcttc aaaactggaa tcctgtggcc tctctttttg
     1261 tttttgttta tttctgttta tttgtttatt ttggagacag ggtttttctg tgtagccctg
     1321 gctgttctgg aactcactct gtagactagg ctggccttga actcagaaat cagactgcct
     1381 ctgcctccca agtactggga ttaaaggcgt gcgccaccac tgcccagccc tgtggactct
     1441 ggaagggtgg tatgctcaca tttccaggac aaacacaccc taggatattg taggacacac
     1501 tggctggagg aggaatgctt ctcagaaaac ctgccttgag gggaggtgcc tggagagatt
     1561 gtatcctgct actactggct caaggaatcc tacaatcagg gaagcccctg gggtgccaga
     1621 gaggagggaa aaaaatgtgt ctcctcacag gtgatacagt gagaagcatc aggggggcca
     1681 tgggataccg tggggtcctg tggtcctgaa aaaactgtga ccttgcaaaa gctggaagtc
     1741 atggaaggag taacagatgc gcgtgataga agctactggg gccacagtca cgggcgaggt
     1801 gggggaccgc tgctgagcgg gggacctggc gggccccaag cctatgtgag cgccggcatc
     1861 ctcacgcagc aggctgcttt tatcgcagca tgggggtgca tgggtgcatc cgagcaagcg
     1921 cgcaggatta tggtccatcc tttcgctcgt gctgccatcc cactgctttg tgtctgcctg
     1981 gggatttcgg ttcgcgaagc attagcatac cattatctcc tcagacaaca gggagagaag
     2041 gtgggcatgc attccggtct ccgtgtgaat aatggaaaat ggagagagac aggggaaagt
     2101 taaatgtgcc acgaaggaca ctgaagctta cagaccctgc tttgcctgac ccctgcctgc
     2161 ctgaggactg ccaggaagat attaacagag gccataaaat tacagccatt acgtctgtct
     2221 cccatgctca cacagtgccc ggactgtctg tgccttgact ttaggcaatc ttgagtttaa
     2281 tttacacaca ctgaggtgca gcaaacccag gctttccagc tggatgcatt ttgacaggca
     2341 tctaccccga tgtgcccatc atccaggttc agatcaaccg ccccgaattc caaacccttg
     2401 ttctcctttc ccatcagtcc tttctcccta gagacaccta gcatgtgagt acctgttatg
     2461 ggcgacccct ttggccattt cttaaacttt agttgtatgg agtcagagtc cctgaaacct
     2521 gacacagtgt ctgcccttta acaggtatcc cgctggaaga aggccttcgt ttctaaacta
     2581 cctgagagaa tgttggtaca aagaggagaa ctgaggctgc gggtttacag gctacagagt
     2641 gaagtcatgg atcctgggcc tgccgggccc catattccat ctcctggctt ccaacagggt
     2701 agtttctttc tgggtttctc ttgtagctgg gtctcctaaa cctgggcagg cagtgccccc
     2761 tgctgcctga aggcctgacc ctagactctt tgcaggcaga gaggatcaca caagtctgga
     2821 gtccagtgac tggcacaggg caggaccctg tgaaacagaa gccacttgtc attttatttt
     2881 cctttggaaa gagtttttcc gtaggatgca gggattttct ttatcccgaa taaacacgcc
     2941 gagggagaaa gccttttggt ggagtactgc tgcccccgtg tggctacctt aagacactac
     3001 aacagagtgg gactcccaag acgaaagttt ttcaggtttc aggttctgcg acttcctagg
     3061 accctgagag acagatgtcc actagggtta tctcttgaca cttaaatttc ctcctggagt
     3121 ggaaatgaga gcgaatgggg cttcttgtgg aaggtatctg ttggggaacg agctttagct
     3181 gtgtgtgtag gcagctgcct ctttcacaac ctgtccagca caagctggac tggacataga
     3241 ctttctccct tgaactaagt atatagactt gtctaatttg agggcaatag aggtcagggg
     3301 agggggactt cttgggagtg gagccgcagt gcctccccag ccggctgctg tgaatgggtg
     3361 ttaccaccaa gacctgcaag ccccacccaa tgtgctctcc gtggccttct tttctgcacc
     3421 tgtggttggg ctgcggtgta acggcccagc ctacctcttc tgggcacgtg gcccggagag
     3481 atcttatctc taactctgta tttttaactc ctctttccag aggatgtggc ttctgcggga
     3541 gagcttcaaa gggggacctg tttgcccatg tcagcaacct gacgtcatgg aagtgggagg
     3601 ctgttcagac ccaaacacct ggagggaagt gggaaacttt ttccatttcc tgttctactt
     3661 ctggctattg attcactatc tgaccagctt acccatttca gggtgcagct gtctgtgtga
     3721 ttcttggagg aaatcctatg ccttgtgtgt ctgtatgaaa ggagacagct gtgttaccca
     3781 tgtggctttt acccaaagca gggctggcga tccttcagac tgccccccac cccaccccac
     3841 ccccacccgt agccagtaca tgcaagagtt acagtatttg acaggtctct cacactcacc
     3901 tctgggaaat atccctcggg gacccagact tcaatcttgt ccagggcggg gtgggctgga
     3961 tatagacttc ctgctttgta aaccggaagc ccttcttccc acatgttgct cgaggaacta
     4021 ggtttcagtg ggtttgtgtg ggtcactggg gaaactgaaa tggacattaa gtgacctttg
     4081 gccctggatg ctggttctca tgtaggacca gagtctttag acttttagaa gccagtgttt
     4141 cctggtaact gtcctgcagg attcagtcaa cagtcatgag gctaatgtga gtctgaactc
     4201 ttcaagggcg caccactcaa gctctgatta ttctaatagt ttttgccaag ctactctatt
     4261 ccttgcaaaa ccgagacagt ggctcacctg gcctctgtta acgccaccac attccctctc
     4321 cagctcaggt ccctcccagt gactgtgttg accgtcatgg ggtcatctgt tggtatctgt
     4381 tgggtgcaag gtactgcact aggtatggag gagctactgg tgagcaaggt attcctccta
     4441 tccgaagggt catctatagt cccttgccaa catgtcactt ggtaataaaa caatattaga
     4501 tttgatatag acatgagctt tgggagttgg attttatggt ataccccaaa caaaaatgag
     4561 acaaaactaa acccttgaag cctcaggggg cggcctgagt ggtcagagca cacacctgtc
     4621 cagcctgcct gtttctggac tgaactgtga ctccagctgc actggacaac gttgcagaac
     4681 actttccaca gttctagcct tggaacgtga agggtccatt tttttttctg ccttcagagc
     4741 tggcctgcag aaagcccttg agggagtaag ggcatttatc tagactctag agatacccat
     4801 tttacagctg gccctggcaa ggagtttgct ggggtatggg tgccttctct ttgggagttg
     4861 gtccagactg gtctgggagc agtggcagcc ttgtgaccat tgtcatgtct tcaggtttcc
     4921 tcagtagcct cttccagact aagcagtgag agatccagag tccgacgtgg aagagtctct
     4981 gcctgttccc cacacttgtt cagctcctgt ggagatttag cctcagctgt gggagaaagt
     5041 tcttgttctg attggatggc gatgaagttg actttgatct atcagtggcc tcctgtctgc
     5101 cttgtccagg cctcgcttga gttggggctg gaagctatct atattcactt caccgctgtg
     5161 ggaggccctt caaatccctc ccacgtcacc tcactctggt cctcaggtga cgttagggtt
     5221 cttgtgacct ctggatgttg ctctctgggg acactgggct agcatggttc aaccgaggct
     5281 gggcctcttt cacgtttagt gaggaggtct gtctttgatt cctatactac cttctaggct
     5341 tcttactctg tcccattccc acaaggtccc ccttcagcct ctgcattatg gcagccagtt
     5401 tttaggagcc cggcatctcc tcagccaccc acgctttctt tgcctggatt tttttttttt
     5461 ttttaataga acaaagtgaa ttaccacttt aggagcaagg aaaaaaaaaa gacaaactaa
     5521 tttgccctaa gttcttaatg gttgataaag gcttgctgca ctaaccttcc catgggcact
     5581 ttcatctaat acagggaaga gaatggttta attaaaagaa atccccagtg gtagtctttg
     5641 gcatcgtgtc agacactcga ggaggggcgt gggattgagg ctcttcgccc ctcctcccct
     5701 gtgtgtgagg gtggccctgt catggtggtg tttcctgagg ccgtccccgt cactggtctc
     5761 agaaggcttc attgtgggct agagatgagg cagtttagaa atgacggggt tctgcggtca
     5821 ctctaatcag gggtggccgg gagagggaac atgggttttt gctgtagtct ccagtcgaaa
     5881 ctgacatttc atggctggtg accctgaagc tcacaggagg gagggacatg ccctcgatca
     5941 cactcgaaag ctggtcagcc ccatctctat gagaagaagc aggcctggga gtcacagctg
     6001 ggaggattag catatttctg gctagtgtgg tcatagacac acagttgaac cagggaaaag
     6061 ctgggtggtg gcagtgggtg ggggtggggg agtcctggga actagtaagt actcccgttt
     6121 ggtgtgagca gttggtctct gaagtgtgtt tccatcagaa tccctgcctg cccttgggga
     6181 aaggagactg ataggagagg ctccctcttt gctgtgggct gtgaaggcag agactctcat
     6241 ggctaggaag agctgagaat gcctgctgtc cttggccaag tcccagacag tagatttgta
     6301 aggcagaaca caggttcagt agcaaggcag tctgatggga gagcaagggt gtccctggag
     6361 ggcaggagcc agtgggtgag aacctggagg acattcctca tccttgactg gtaaacttca
     6421 gctacaggtc agagcctgcc catcagcccc ctgcggtcag cctcttaatg ggtgcggcat
     6481 gagcggggac tcacccctgt aaaaggagct tcctggccct tggtacagct tgcttagtgg
     6541 aatccgcccc cacacccccc aagacagggt ttctctgtgt agccctggct gacctggaac
     6601 tcactctgta gaccaggctg gcctggaact cagaaatccg cctgcctctg ccttccaagt
     6661 gctgggatta aaggagtgcg ccatcacgcc tggccgctta gtggaatttt taagaggtca
     6721 ttccaagagg ttgaatggag agggtttgtt gaggtaactg ggcaagtgtg ataggcacat
     6781 acttcggttt ctctctgcat gtaaatgccc ccaccaagac ccagtcaaat aggatcacag
     6841 ttttggtttt tctgaatcac ctttaatgat gtcatgggca gcagaataat atacaatgtg
     6901 gctcaataca cacagtagca ggagggtcta gacaatgaga taatttctac accttgatga
     6961 aagagtaata tgcatttctt tatggatgct taaaaaagta aacccgccaa aaaaaaaaaa
     7021 acccatattt tttacaacat tctccaagag attttatagg ggagtctgcc atgggggagg
     7081 ggtcttcaga tccaggagaa gccccgcccc tgccgttttt tttttttgtg aatggtcagc
     7141 agcagtgagg gagctgagtg ggtgcggcag cagcgcttag ctctctagca accttcctca
     7201 caaatctggg agatccaggt gggattgtga atcagggcca accagacccc agacagccgg
     7261 ctccttttgg cttgaagagg gagcggaggg gtgaggagga cggaacttgg gagtcaggct
     7321 ctgtcagtct tgcagacctg gagtgaggaa gtgcagaggt caggcttggc atggaggact
     7381 aggaccccca ggggtgtgat gaggatgaat ttcttagggg ctgtgcaagg tgaccatgga
     7441 agcatgatga ctctagccag gtagagggct aatgagtata gcatcattga atatcaagca
     7501 gagagagctt gctagggaga aggcacacag gaagggagtg tacactccag aaggcagggc
     7561 cagagacacg tggtacgtgt gcatcagggt ccggagtttt ctcctcctct gttctttggc
     7621 tgtgtgagcc ttgatgtgac ttggcctcac ttccatcatt tctaaatgag catgacctga
     7681 gtatcttagg agagtcactg ctggaccaag gtcgctgaaa ggattgtgtt ctgcagccca
     7741 ggtgtttcag ggcactgttg caacagtgtt acaacttgta atgggtaggg ggtagaaaag
     7801 ttctgtgggt ccagtcttag gatttaaaag caaattttaa atcccagggc gatttgcgct
     7861 cagggttggg tcttgccctg aatccacacc tctaagcctt ctccaggctt tcatgccttc
     7921 ttacctcaac tggaccacag cctcagcgtc atgagcaggt taaatcccgc tactttcagc
     7981 aggaggattc ggagtcccat aactgacagg ttttgaaagt ttaggttcat atctgtaaag
     8041 ccaagaggtg gtagccatta acggtatgtg catgagaggg cagtacagca actcacgaca
     8101 ctgtggactt tcccaagagc tggttaggca tgcttgggtg tgggtaactt caactcccag
     8161 gttgcttctt tgagcttctg cacacacccc aaggcaaagg tatttgtata tccctccagg
     8221 gtaagagcat tgtgttttaa gacaaaaacc accaaaagga cttagtccat ataatattct
     8281 gaggccctgt acggatgggg gtgggggtat gggtgtgggt cggggggggg ggtagatgtt
     8341 cagaacttgt cataatggtc tagacctggg cctctcagtg aaaccacaga tgtgatgact
     8401 cagtaactag tcacgtggaa tcgaggtggc attgagacaa gacctttgcc tgaggctatg
     8461 tgagttctat ttcctttcta gaaaggaatt cccctgagat acagagaggg ccagtgtcca
     8521 tagagtgaca aggttagttc aaggctgctc tgaagggact cacaagttcc acagtagcat
     8581 atgaagtggt tttctttggc tattctcaca gaggagccaa tacaagagac accaaggccc
     8641 gagagtgact cactggcctt gtgaaactgg aggcaccgct tgttaagtac ttagaatgga
     8701 aaatgacgtt aaccgtgctg gggattcaag acagaccctg cgaagcttgg ggcaggactg
     8761 gaaatgctgt tcccaccccc agatccaccc cctcccctcc cctgtgcact aaaggtcatt
     8821 actggcccct ccccaccccc cagcatcact cagtgttggt cagacccttc tcttacctgt
     8881 ttcaaagctt ttctcggtca acgtggcatc acagggaacg tctaagaggg aaaacaaaga
     8941 catttcctta gattctgaag cgtgttaact gtcccaccac aggttgttgg tcaggaaaat
     9001 atatcacagc ttttctgtga tcctttggga gagagctggg ggccatattg cctaagggta
     9061 tctgctggag caaaggccca acaccctggc ttatgggcac tcttttattc tttcaacaac
     9121 tatgggctgt gcaaagtacg tgctgtatga catgggccaa aaagtcaaga tagtccctga
     9181 acctggaggt ttcagtctag agtcccgtaa agacagcaag acatattcac agtgaccagg
     9241 gccatgtatt ttcatgtaca cctcccccca atagtgttga tacacaaatt tgctccttta
     9301 ttatctctta cttagtataa ggtcccatcg accgagatgc tctgccactt cccttgatgc
     9361 ccattgaccg atctctgtgt ggttctatct gtgaaaccta agtgctgttg gctgctggag
     9421 tcctgccatc acctggtggg gcttccttca actttctatg ttctctgtct tctgctggat
     9481 ctacagtctg ccttctacac accactcaat cttatgttta tttagttgtt ttgggagaca
     9541 gggtctcggg tagtccaggc tggccttgaa ctttctgtgt aggtgatgat gactttgaac
     9601 gtctgcttct cttgcctcta cctgtgatct gacccccctg tctatggctt acaatccatt
     9661 tcctgcagct tctccttatt ctccggattc tcctttcttg gcttctgaca ggctcctctc
     9721 tggccccagg cagggctggt cccttcttat ctattaactt gtagtgtgat agcattttct
     9781 ctgagccttt ctctaagctg ttcttgcctt aactctgagt tattgcatat attaattgca
     9841 gcctggtgtc atttacatgt ccctctgctg acggatggtc ctcctggatt agagtggcgt
     9901 cccctcctcc tcatctgact tctgtactca cactacttgg cagacataga aatcagggca
     9961 ggcggctggg ggtgggtggg tgtgggatgg gcaggaaggg aatggaaact gtgcctggga
    10021 gaacagagaa ggcacttggc tcaagagaag acaggaaggt gagcctcaga attgtgagac
    10081 caacgctggg ccaatgtcct ctgtcatgtt ctcttgtctg ctttggtgac ccgttaggac
    10141 tccttagtga cacaggatgt ggattttgct gcctaatgat gagggtgaga tgacccaaag
    10201 cagcttttgg aaaccataga tttgagccag gaggagactt ctggggcacc actcacctga
    10261 actggggtag gtggcgttgg tctctttgaa gatatcttgg caggtgaagc ttgtctggtt
    10321 gctccaggca atggccccat tgctcttgga atccatagct ttcatgtcca gcacagtttt
    10381 gtcagtgatg aacgttccag attccatggt tttcggcaca ttgatttggg agtcaaagtc
    10441 ggtgaacagg cagagggtgc tgtcctgaga ccgaggatct tttaactggt acacagcagg
    10501 ttctgggttc tggatgtctg tgggccaaga ggactgatgt tagaagattt tttgggggtc
    10561 ctaacttagg tcagaactca gatgccagtg acaggcaaga gcggaactct ctggactagc
    10621 cagtcttgtt cacagacttc acattggaaa tgacagccta ggatctagct gcccatgatg
    10681 gacacaggct tagggacagg cacaagtcat gagtgccagc caagatctct agctttcagt
    10741 agtccctctc cacggggtcc tttctggtat ctgggaaaac cagaaaggaa ggtggaacca
    10801 ctaaaccatc ttgtcatttt tctacttaac aggatcttca gaaccccagt gggtccagtc
    10861 aaacaatttt gttttggcag gacaaaaaca agctcagaaa acagagactt agatgtcttc
    10921 tctagagtac tctcccccag gtaggctgga cgttggtgag aaacgaaggc gtgtgatatt
    10981 ttaacattaa gttggaaaat ctctcacact accgggccat ttttcttctc tcttgataaa
    11041 tgttttgaat agcactcaca ccagagccct gtctctacaa tttactccgg tcagcctgga
    11101 ttaaatcaac aggcagctga agtcgcagaa ttttcacctg taagagggac accaggctgt
    11161 tctgagaaat gactatttct tcaaataaaa ataaccgagt aaaccacaca ccgggcactg
    11221 aacaaacgtg cccggctttc accatgaaaa cataccactt agcaaaccaa tcacactgtc
    11281 tctttattcc aggaaagcgg gtgctacctg aagacaattc tttcctttct ccaagggatt
    11341 ttgtttttaa tgtgactctc tcagactatt tccccatgcc ctccccggtc tgaggaggct
    11401 ggctggagag attaacacag cggaccacct agagagtttc cagcagggct gtgatccaaa
    11461 gctccttccc ctgtgggtct cccacagttc ttaactgatc acaggagcca accagggtgg
    11521 ccttggcaat gctgactccc agctggatcc taaaccaaag gaatcaggcc ctggtgccat
    11581 tgctcaagga tgctgagtta agacaagagc tgtagcaagc aaggtactgc ctcggagcag
    11641 cagagctggg gggggggggt gccgtggtgg gacatggagt tctggcccct ttcaggcaaa
    11701 tatttaaggt ctcaccacag agacgtcctg gtgtttagtc cccttcatgt ccagccccat
    11761 agatatctac tgcaacccca cctttacctc ctgaaaatga gaaacaaatc ccctttaact
    11821 agaggtcttc agggttcctg tctctcagag ccctgcagcg agagacattc tatttctacc
    11881 tggtcagaaa aacacatttt taccactgat ggcatccgag gtcactttag caagactgga
    11941 tcaatttctc agctacaggt tcagccgctc tgattcctaa cttgccatca taattcaaaa
    12001 catacagagg cggcgcgtgt ctgagtgtat aagcgaggta ggtccccagg ggcattgcag
    12061 tgatggagag agtccctcac cactaaatct gtgctctgtt gttaagtacc ggaaacctcg
    12121 gaatgagagt tagtgacaca gatgtgggtc ctactcaggc ttgggtagcg tcaggaaaac
    12181 agcaggcagg cttgtcagga aagatggagc agttatctgc acaccagtcc taatgcttcc
    12241 ttacagtaac accccctgat tgtcttccaa ggaggctggg atgaactcac ctaggagtgt
    12301 gatgaagacg aagggggcgg ggggggggga attcctgaca cccctcacct cacatctagt
    12361 cttgcttact aaactggatt ctcagttttc ttgtggctgg ccctggtccc aatctctgta
    12421 ttccctggga cgttctagta ttttagtatt tcttattccc actgtctaag aaggttcctt
    12481 tttttttttt ttccttgttc tcttagtctc tttttactat cagatctgcc tacacaagcc
    12541 aaaaggcctg tgtcttgttt atcccaagat caaatcttcc ttcttgttgc cccttggttg
    12601 gaatcctgcc tccctcctct ctctgactta cacagatctt atcaacctgt caaggctcaa
    12661 caaatctttt ctagtacaga aagcagcctc agtctagggc accttctcca ggaccctgac
    12721 cttctgaggc tgtttatcta caccagcagt tgccacccct taaatagttt gtaggttgtt
    12781 aaatgtacag tagttctgaa catttgtcta gtttctatgc tgtgtgcgga caggtcagac
    12841 atctatctgt cttgggcttt tttattctca gcctaacatt acctagagtg atagctactt
    12901 agaggcattt aaaagtgtcc attgaatgat taaagtcatc ttttaatgag tttgacctag
    12961 agaaaacaca gatatttaag attgatagtg atgcctcccc ccttcacaga cctccgtaaa
    13021 acaattagca ggatacccca gttagcctca ccctgccatg ggaacagcgg acatctctct
    13081 ccagagagag ggttaatgac cgtgccctac cgggttgcaa atggtgccac ttggggtaac
    13141 tcaccagata ttaccgtcac ttgggtccct tccccgaatg ttaatttacc acttagtcct
    13201 ccagtattca cactgcaggt gacaccttta cagaagccta ggcgacctac ctactcctta
    13261 gatagaaaga aagtctctca gggtggagaa tcccaggcct gaaactgagc ccctttgctg
    13321 tcttgcattg gaattctgtc tctgctgtag acagtccttt cccctgcctt gttctactgg
    13381 gaggctccac attgaagtaa gcgaagtgat ttccacaaat tcaggagaat ttatgagatc
    13441 aaaacttcct gctcagagca gcagtttctc ggatactcta atcgaaggat ctagattatc
    13501 aaccgcgtta tgattgccaa gggtccagag caaggacaat gtttcttcag gacttagtgc
    13561 tgggtgggat gggttctttc tagaaacatt tgcaatccta cagaagaaga taccagaact
    13621 ccagccagtg tatcttggac agagaggcag aggaagttct tgtaaatcct atccttgact
    13681 cattctacgt atatggtgga tttctgagtt tggtttcggc tccaaagatt agtttagagc
    13741 tgtagctgaa ttcccagtcc agtccagtcc agtccagtcc agtccaggtc tttctgacac
    13801 cagtcagagc ccactggggt ctcaggatat ttccctaaac tgtcctggag ttcttggtcc
    13861 cttacaactt ctatttacca aggaagctgt tgtttcgctc tcttgacaac agtataaacc
    13921 tcgtgtggca aggccatgat cagatcatag taccgaggat gcattacact ctcccaatgg
    13981 aggagttttg tttgttgttg ttgtgtgcag gcaacgtctc acatgatacc agatgatgtg
    14041 gggagatcac tttgttgttg aaagtggaaa cactgttaaa tatagccggt gaattaaacg
    14101 ctattaaata gagccagaat tcagcacagg cctatcctcc ttctcctcct cctcctcctc
    14161 ctcctcctcc tccatctccc tccctcccta tctatcttca caaaacagag tacactggcc
    14221 agtcacggag ctccaccgcg gtggcggccg caactcagtg ggagagaatt tcagaacatc
    14281 agaaaatgga acatcactta ctagggagaa catgaagatg tgtccctttt ccaaagatga
    14341 ctttgtaagc atttgtggaa gagctcacag cacagaagta cacagccgag tcgctccagt
    14401 gcacagaggc tttccgaagg tggaaggaag agtcgctctt gctgaactca gcctcgaagc
    14461 cgttcactcc ttgaaccact gggtttcccg aatagtactt gaggagaaac tggaggccat
    14521 ggccatggtg ctggatgtac caggagaggt aaatggatcc accataggaa tagttgcatc
    14581 tcagctccac cgagtctcct tcgaagacag tgacatgggc atcagactgg gaaactgtct
    14641 gtgtttggac atccaggaag aaatgtatcc cgaggaacga gatgagaacc aggagcatgg
    14701 tggcagtgag aagcccggga gatgtattca ggaaaatctc cctgtggaca aggcctggag
    14761 actattaact gagagatgct cctcccttgg caatgactgc tctgtcgtcc tttctactta
    14821 caactgcact tctgagagct gcaaggtata aaggctagct gaagtgacag gatttttttt
    14881 tttctttctt gcccttatag acattcagtc tatgcaacag tgcccccttg tggtcaaagg
    14941 tggaaaaatt aggccttctt tggtgttagg ttcattgaag caataaaatc tggtgaaagt
    15001 gaatcttaag cagatgatct aattttatga gttaagtagt actatttaat cttatgagct
    15061 agaaaatgct accgtcctcg ttttataaac tagaaagctt aagaatggaa gaaagtgtgt
    15121 gtcacagcaa gtgacaagcc tggagtttct ctcattacta tggccaggaa ggcccatcag
    15181 taatgactaa tgactatgca catttatcac aacagccttt tacagtgtta gctgtcatat
    15241 ataatgaaca ggaaatcaaa actttataga gctaactagg tttcataagg tcacagacca
    15301 ccagcttgtc tgctcctctg aagacccaac agtctatagg cctcccagga aatctgcggc
    15361 acttttattt tttattgcac atcttattag tgaaggaaaa agaaagaagt gcaaacagtg
    15421 acgctagcct tgtggagtga gtgggagaac cgctctgcac aggaggagga acgactgata
    15481 gggccaacaa aggaggtaca atcagtgatg gccatggaag tcctaagtaa attctactta
    15541 agcagcggtt gggtggggag gtcctgacac atgtccccaa acaaagatct ctgggaggac
    15601 agaaccacta gcaccagggt tatggaggag cacagaacca caaacccgag aatcaaggca
    15661 aaactgtcag ctaaagcctt gcttctgtag tcagagtctc cttagggaac agtgacagtc
    15721 agaggatgcg agccaccgct gcctttagtt aaaagatgcc ctgatggttg cttagaaagt
    15781 aataaagaaa gaacaaaata attattgaag gaccataaaa gcatcaggca agcctgttta
    15841 gattagagga cagtccagct agactctaaa gatactgatt ttttttttct caaaagtgat
    15901 tttttttttt ggcaggtgat ttggggaaat ggaatgattt ctttccctga atgtagaagc
    15961 aactctttga cctaccagta ccatttgcaa atctgtttag aactcagcct ggcatagcag
    16021 taccccataa tcctgctacc agcttgtctt agttaggatt tctattgctt cagtgaaaca
    16081 tggaggccag aaagcaagga ggggagggaa gagttagtgt ggtccaatca aggaaggaag
    16141 acagaactgg aatgcaaacc ggacaggaac ctggaggcag gagctgatgc agagtccaag
    16201 gaggggctct acttattgcc ttgctctcca tggcttgatc agtctctttc ttataaaacc
    16261 cagatctgcc atcccaggga atggaacgac ccacaatagg ctgggccctt ccccatcaat
    16321 cactaatgaa gaaaatgccc tatagtctaa ctgcagccca ttctagcacg gaggagcttc
    16381 cccgaccccc gccgtgttgt ttgacctcta tggtgaatct attcttgtgt tctaatttta
    16441 acagcaacaa tcataaattc cgaagtatca cataattgaa caagtcagga aatctttggg
    16501 catctgtttt tctccatcag ttactcttgc tcccgtatct cgggttgttt tctaaacaca
    16561 aaaagtgaag agcgatggac ataagttagc aacaaagaag ggggagggag ggagggaagg
    16621 agggagtaat ggaaggagag agaagggtgg gagagagaga gaaggggcag agagggagaa
    16681 aacaagctca gacatcctct ctgccgtgtg tagtcatgcc gagacttatc tacaagacat
    16741 gccattactt ccagcctcca ctaacccata taacagggcc ttatgtcatc agcctgccag
    16801 tccttctgag ccctcctaca ttattacagc aggggaaggc tgcatttccc ccagacagtt
    16861 cctactacac cacgttcaaa ccagtttaag ctatgaacac tagagggcag caaagccagc
    16921 gttaagtcac aaaagttagt tggccatcag gtcggggatc ctctagagtc gacctgcagg
    16981 catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
    17041 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
    17101 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgattccgt
    17161 tgcaatggct ggcggtaata ttgttctgga tattaccagc aaggccgata gtttgagttc
    17221 ttctactcag gcaagtgatg ttattactaa tcaaagaagt attgcgacaa cggttaattt
    17281 gcgtgatgga cagactcttt tactcggtgg cctcactgat tataaaaaca cttctcagga
    17341 ttctggcgta ccgttcctgt ctaaaatccc tttaatcggc ctcctgttta gctcccgctc
    17401 tgattctaac gaggaaagca cgttatacgt gctcgtcaaa gcaaccatag tacgcgccct
    17461 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
    17521 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    17581 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    17641 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    17701 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    17761 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    17821 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    17881 ttaacaaaat attaacgctt acaatttaaa tatttgctta tacaatcttc ctgtttttgg
    17941 ggcttttctg attatcaacc ggggtacata tgattgacat gctagtttta cgattaccgt
    18001 tcatcgattc tcttgtttgc tccagactct caggcaatga cctgatagcc tttgtagaga
    18061 cctctcaaaa atagctaccc tctccggcat gaatttatca gctagaacgg ttgaatatca
    18121 tattgatggt gatttgactg tctccggcct ttctcacccg tttgaatctt tacctacaca
    18181 ttactcaggc attgcattta aaatatatga gggttctaaa aatttttatc cttgcgttga
    18241 aataaaggct tctcccgcaa aagtattaca gggtcataat gtttttggta caaccgattt
    18301 agctttatgc tctgaggctt tattgcttaa ttttgctaat tctttgcctt gcctgtatga
    18361 tttattggat gttggaatcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
    18421 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
    18481 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
    18541 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
    18601 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
    18661 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
    18721 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    18781 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    18841 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    18901 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    18961 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    19021 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
    19081 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    19141 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    19201 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    19261 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    19321 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    19381 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    19441 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    19501 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    19561 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    19621 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    19681 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    19741 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    19801 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    19861 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    19921 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    19981 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    20041 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    20101 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    20161 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    20221 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    20281 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    20341 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    20401 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    20461 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    20521 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    20581 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    20641 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    20701 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    20761 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    20821 acaggaaaca gctatgacca tgattacgaa ttctgccttc aaagtcaggt gaccccccaa
    20881 cgaccccatg cctcacagtt gtaaaaagca aacaaaccca gatctctgct ttggctctgt
    20941 ggatctgctg tttgacgtct ggtctcatgg gcaacgtctt agctttcttt cacattggaa
    21001 aagaaaaaag gtggaaggag actctttaaa tgttaaggga tggatgaaaa accctaaaaa
    21061 ctcctttgct tgcatctcct gaaaatggcc aagaaggggg aggtctattg catcccattc
    21121 tgggctccaa atgctatatt agagaagaca acatgagaac aattcagaca tggacttact
    21181 tggaacgact tggagttttg tcccagttcc aaacctctgg taagtcccag aatttgcact
    21241 gtaagtgagg tctttacaaa atggcttctt tcccttcctg tttccatcag agtaactctc
    21301 aactcataca cttccatgag agagagaggg tgtttttaac cagtgcctgt agccacagat
    21361 ccacatgaac acacaccatc tcttcatctt aaaagagtct atgactgacc cagctttaat
    21421 atttttttgt tgttttatag acccatattt tatatgtgga tagcacttca gaatctgtaa
    21481 aatattttta catacattaa cttatcctta tattaactta tgaagtatta ttaaacactt
    21541 tctttttaca gatagagctg ggccagtgca cgagtcaaag cagatttagg ggacctggat
    21601 gtgtttcagc tgagctgccc tttcttcagg atcagctctg cgggagctat tatttctagg
    21661 gttcctacat gacctgtctt gttcacagcc agttctgcac ccttatttta acagtttccc
    21721 catacaagga atgcgcttgt ctgcatgaac cattaattct ttgaccactg tgcctcacaa
    21781 atactgtggc aagttcagtg ggccttagca tccctattaa gcatctcttt agtgatgtct
    21841 tgtcccccaa atgaatacat tgtaaatcgt ggtcccgtgc tgtctgacaa aaatgctcaa
    21901 cctgctgtga tcttagattg atgtgatcag cattcagctc tcttctctct tgggagttct
    21961 gctagggatt cccccttttt gattactgga ataaaggcta agccatggaa ttaagcccgt
    22021 gaaagcctct ttacaaggct caacaacagt ggtgccatag ctgtagctaa agcctgagag
    22081 gatagcagct gatattctcc agtcaattct gaaacatact ggtttttaaa aagatttgta
    22141 caatttcacc aagtcgacgg gatccacggt cctctgcctc tgacagggtg tggttttctt
    22201 tctgtatgcg tgcgtgcgcg tttgtgagtg tgtcagagga catcttggca ggagtcggtt
    22261 ctttccttgt accatgtagg tactaaggtt caagcttggg tcaccagggc gtcactaggt
    22321 gcctttgtat acagagtcat cttgtcttct gattttgcct tatcactgaa cttctgaccc
    22381 acggggctgg caaggtggct cagagagtaa aaaccccttc tgcgcagccc cggtggtctg
    22441 agattgaccc cccccgcccc ccagcttcca
//
