LOCUS       sequence-246832-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GB1 fused to GyrA intein at the C-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Liu J, Ekanayake O, Santoleri D, Walker K, Rozovsky S
  TITLE     Efficient generation of hydrazides in proteins by RadA split intein.
  JOURNAL   Chembiochem. 2019 Jul 2. doi: 10.1002/cbic.201900160.
  PUBMED    31265209
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2019 Jul 2. doi: 10.1002/cbic.201900160."
COMMENT     Sequence Label: GB1-GyrA
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      217..303
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     438..457
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        438..456
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    457..481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             496..518
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             535..696
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     gap             789..802
                     /estimated_length=14
ORIGIN
        1 ccnnnataac tgccannann aattccccag gcatcnaata aaacgaaagg ctcngngaaa
       61 gactgggcct ttcgttttat ctgttgttng tcggtgaacg ctctcctgag taggacaaat
      121 ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc
      181 ccgccataaa ctgccaggaa ttaattcccc aggcatcaaa taaaacgaaa ggctcagtcg
      241 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
      301 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
      361 cgcccgccat aaactgccag gaattgggga tcggaattaa ttcccggttt aaaccgggga
      421 tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc
      481 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggg tcagtacaaa
      541 ctgatcctga atggcaagac cctgaagggt gaaaccacca ccgaagcggt cgatgcagcg
      601 acggctgaaa aagttttcaa gcaatatgcg aacgataatg gtgttgacgg cgagtggact
      661 tacgatgacg ccacgaaaac ctttaccgtg accgagggtt gcatcacggg agatgcacta
      721 gttgccctac ccgagggcga gtcggtacgc atcgccgaca tcgtgccggg tgcgcggccc
      781 aacnnnacnn nnnnnnnnnn nng
//
