LOCUS       sequence-246834-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PhoRadA intein split at residue 126 from the N-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Liu J, Ekanayake O, Santoleri D, Walker K, Rozovsky S
  TITLE     Efficient generation of hydrazides in proteins by RadA split intein.
  JOURNAL   Chembiochem. 2019 Jul 2. doi: 10.1002/cbic.201900160.
  PUBMED    31265209
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2019 Jul 2. doi: 10.1002/cbic.201900160."
COMMENT     Sequence Label: MBP-TEV-Gly-RadA∆C46
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     566..589
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             605..625
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gttcnagtnn ngaaaacggc aagtacgaca ttaaagacgt gggcgtggat aacgctggcg
       61 cgaaagcggg tctgnnnnnn ggttgnnngn taaaaacaaa cacatgaatg cagacaccga
      121 ttactccatc gcagaagctg cctttaataa aggcgaaaca gcgatgacca tcaacggccc
      181 gtgggcatgg tccaacatcg acaccagcaa agtgaattat ggtgtaacgg tactgccgac
      241 cttcaagggt caaccatcca aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc
      301 cagtccgaac aaagagctgg caaaagagtt cctcgaaaac tatctgctga ctgatgaagg
      361 tctggaagcg gttaataaag acaaaccgct gggtgccgta gcgctgaagt cttacgagga
      421 agagttggtg aaagatccgc gtattgccgc cactatggaa aacgcccaga aaggtgaaat
      481 catgccgaac atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa
      541 cgccgccagc ggtcgtcaga ctgtcgatga agccctgaaa gacgcgcaga ctaattcgag
      601 ctctgaaaac ctgtattttc agtccatggg cggttgcttt gcccgtgaca ccgaagtcta
      661 ctacgaaaat gacaccgtcc cgcacatgga atcaatcgaa gaaatgtact cgaaatatgc
      721 gtccatgaac ggcgaactgc cgtttgataa tggttatgca gttccgctgg acaacgtgtt
      781 cgtttacacc ctggatattg caagcggcga aattaagaaa acccgcgctt cttacatcta
      841 ccgtgaaaaa gtggaaaaac tgatcgaaat caaactgagc tctggctact cactgaaagt
      901 taccccgtcg catccggtcc tgctgtttcg tgatggtctg caatgggtcc cggcggccga
      961 agtgaaaccg ggtgatgtgg ttgtcggtgt tcgtgaagaa gtcctgcgtc gctaaggatc
     1021 cgaattccct gcaggtaatt aaataagctt caaataaaac gaaaggctca gtcgaaagac
//
