LOCUS       sequence-246835-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PhoRadA intein split at residue 46 from the C-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Liu J, Ekanayake O, Santoleri D, Walker K, Rozovsky S
  TITLE     Efficient generation of hydrazides in proteins by RadA split intein.
  JOURNAL   Chembiochem. 2019 Jul 2. doi: 10.1002/cbic.201900160.
  PUBMED    31265209
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2019 Jul 2. doi: 10.1002/cbic.201900160."
COMMENT     Sequence Label: MBP-TEV-Gly-RadA C46
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     797..820
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             836..856
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1001..1018
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttaccnggga tnccgtacgt tnnnanggca agctgattgc ttacccgatc gctgttgaag
       61 cgttatcgct gatttataca aagatctgct gccgaacccg ccaaaaacct gggaagagat
      121 cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca
      181 agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga
      241 aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct
      301 gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat
      361 cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg
      421 gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg
      481 tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa
      541 caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc
      601 ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggt
      661 gaaagatccg cgtattgccg ccactatgga aaacgcccag aaaggtgaaa tcatgccgaa
      721 catcccgcag atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag
      781 cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actaattcga gctctgaaaa
      841 cctgtatttt cagtccatgg gccgtattat cagcaaaggt gaactggaat tccacgaagt
      901 cagttccgtg cgcattatcg attataacaa ttgggtgtac gacctggtta tcccggaaac
      961 gcataacttc atcgccccga atggtctggt cctgcacgca catcaccatc accatcacta
     1021 aggatccgaa ttccctgcag gtaattaaat aagcttcaaa taaaacgaaa ggctcagtcg
     1081 aaag
//
