LOCUS       sequence-246847-       10098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of V5 Dispatched WT HA in mammalian cell lines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Stewart DP, Marada S, Bodeen WJ, Truong A, Sakurada SM, Pandit T, 
            Pruett-Miller SM, Ogden SK
  TITLE     Cleavage activates dispatched for Sonic Hedgehog ligand release.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 31678. doi: 10.7554/eLife.31678.
  PUBMED    29359685
REFERENCE   2  (bases 1 to 10098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 31678. doi"; pages: " 
            10.7554/eLife.31678"
COMMENT     Sequence Label: pCDNA3 V5 mDispatched WT 2x HA
FEATURES             Location/Qualifiers
     source          1..10098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      747..756
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1071..1112
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5364..5390
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5391..5417
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5455..5472)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5461..5685
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5731..6159
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5818..5837)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6028..6049
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6168..6188)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6173..6502
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6353..6488
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6415..6434
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6569..7363
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARFKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6623..6642)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7233..7252
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7537..7658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7574..7593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7628..7647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7707..7723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7707..7723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7720..7742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7731..7747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7755..7761,7762..7779,7780..7785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7800..7821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7938..7955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8109..8697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8189..8208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8868..9659,9660..9728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9491..9510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9729..9833)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9908..9927)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttctgcagat atccagcaca gtggcggccg ccatggctgt gatcagcgga agtgactctg
      781 tgctcctgag caatggcagc atctcgacca gcacctccaa ccctagtccc ctcagcccca
      841 gtgatggaga cctcccagcc cagcacctcg gacccagaga aaccccaaga acaaaagcaa
      901 gcccaaatgg atgcctgcaa cttaatggca cagttaagtc atcctttctg cctttagaca
      961 accaaagaac acctcagacg ccaactcaat gctgccaccc ttgtccatac catcaccctg
     1021 tgagcagcca tagcaatcac caggagtgcc atcccgaggc tggccttgca ggtaagccta
     1081 tccctaaccc tctcctcggt ctcgattcta cggcctcccc cgctttggcc tcgtgtcgca
     1141 tgcagccaca ttccgaatac tctgcatctc tgtgtccaaa ccattcaccc gtgtatcagg
     1201 ctgcgcactg ccttcagccc tcgccatctt tctgccttca tcacccgtgg cctgaccatt
     1261 tccagcatca acctgtacgg cagcacctga ccatcatcag gccgtccaga cctttcaagt
     1321 tcccgagaag ttacgcagcc ctgctggctg actggcctgt ggtggtcctg ggcatgtgca
     1381 cgctgctcat cgtggtctgt gccctggtgg gggtcctagt gccagagctc cccgacttct
     1441 cggacccgct gctgggtttt gaaccaagag ggaccacgat aggacagagg ctggtcacgt
     1501 ggaataacat gatgcgaaac acagggtaca aagcaacatt ggccaactat ccttataagt
     1561 atgcagagga gcaagcccga agccaccggg atgaccgatg gtcagacgat caccatgaga
     1621 gagagagaag agaagtggac tggaacttcc agaaagacag cttcttctgc gatgttccaa
     1681 gtgatggata ctccagagta gtgtttgctt cagcaggagg ggagactctg tggaatttac
     1741 ctgcaattaa gtcgatgtgt gatgtggata attccaggat cagatcccac ccccagttca
     1801 gcgatctctg ccagaggacc actgccgtct cctgctgccc gagctggacc ctggggaatt
     1861 acatcgccat tctgaataac agatcgtcct gtcagaagat tgttgagcga gatgtctctc
     1921 acacgctgaa gctgctccgg acctgcgcca agcactacca aaacggcacc ctggggccag
     1981 actgctggga caaggcggcc agaaggaagg accagctcaa gtgtaccaac gtgccgcgca
     2041 agtgtaccaa atacaacgcc gtgtaccaga ttctccacta tctggtagac aaggacttca
     2101 tgaccccaaa gacggctgac tacgccgtgc cagctttaaa gtacagcatg ctcttctccc
     2161 ccacggagaa aggggagagc atgatgaaca tttacctgga caacttcgaa aactggaact
     2221 cctcggacgg catcaccacc gtcaccggca tcgagtttgg catcaaacac agcttgttcc
     2281 aggattacct cctcatggac accgtgtacc ccgccatcgc catcgccatt gtcctgctca
     2341 tcatgtgtgt ctacaccaag tccatgttca tcacgctcat gaccatgttt gccatcatca
     2401 gctcgctcat tgtctcctac ttcctctacc gagtcgtctt taacttcgag tttttcccct
     2461 ttatgaacct cacggcgctc atcatcctgg tcgggattgg cgcggacgac gcttttgtcc
     2521 tgtgcgacgt ctggaactac accaagttcg acaagcctcg cgcggaaact tcggaggcgg
     2581 tgagtgtcac cttgcagcac gcggccctct ccatgtttgt caccagcttc accactgccg
     2641 ctgccttcta cgctaactac gtcagcaaca tcacggccat caggtgcttc ggggtgtacg
     2701 cggggaccgc catattggtg aattacgtct tgatggtcac ctggctcccg gctgtgattg
     2761 ttctgcacga gcggtacctc ctcaatatat tcacctgctt caggaagccc cagccacaag
     2821 cctacgacaa gagctgctgg gcagtgctct gtcagaagtg ccgcagggtg ctctttgccg
     2881 tctcagaggc atcgcggatt ttctttgaaa aggtgctgcc gtgcatagtc atcaagtttc
     2941 gctacctttg gctcatctgg ttcctcgccc tgactgtggg cggggcctac atcgtgtgcg
     3001 taaacccaaa gatgaaactg ccgtcgctgg agctgtctga gttccaggtg ttccggtcct
     3061 ctcatccttt cgagcgctat gatgccgagt tcaagaagct cttcatgttt gagcgggttc
     3121 accacggaga ggagctgcat atgcccatca cggtgatctg gggcgtgtcc ccagaagaca
     3181 gcggcgaccc tctgaacccc aagagcaaag gggagctgac actagatagc acgttcaaca
     3241 ttgctagccc agcctcccag gcctggattt tacacttctg tcagaagctg aggaatcaga
     3301 ccttcttcca ccagacggag cagcaggact tcaccagctg tttcatcgag accttcaaac
     3361 agtggatgga gaaccaggac tgtgacgagc ctgccctgta tccctgctgc agtcactgca
     3421 gcttccccta taagcaagag gttttcgagc tgtgcatcaa gaaggccatc atggagctgg
     3481 acagaagcac gggatatcat ctgaataaca agacccccgg gccaaggttc gacatcaacg
     3541 acaccatcag ggcggtggtg ctggagttcc agagcacctt cctcttcacc ctggcctacg
     3601 agaagatgca gcagttctac aaggaggtgg actcgtggat ttcccacgag ctgagttcgg
     3661 cccccgaggg cctcagccgc ggctggtttg tcagcaacct ggagttctac gacctgcagg
     3721 acagcctttc cgacggcacc ctcatcgcca tggggctctc ggtggccgtt gccttcagcg
     3781 tgatgctgct gaccacgtgg aatatcatca taagcctgta cgccatcgtc tccatagctg
     3841 gaaccatatt tgtcacagtc gggtccctgg tcctgctggg ctgggagctg aacgtgctgg
     3901 agtccgtcac catctcggtc gcagtcggcc tgtctgtaga ctttgccgtc cactacgggg
     3961 tggcgtaccg cttggctcca gatcccgacc gagaaggcaa ggtcatcttc tctctgagcc
     4021 gtatgggctc tgcgatcgcc atggctgcac tgaccacctt cgtggcgggg gccatgatga
     4081 tgccctccac ggtcctggct tacacgcagc tgggcacgtt catgatgctc gtcatgtgcg
     4141 tgagctgggc cttcgccacc ttcttcttcc agtgcctgtg tcggtgcctg gggccacaag
     4201 gcacctgcgg ccagatccct ttccctacaa aactccagtg cagtcccttt tcccacacct
     4261 tgtctgcaag gcctggggac aggggaccaa gcaaaacaca tgccgccagt gcgtacagcg
     4321 tagatgccag gggccagaaa tcccaactgg agcatgagtt ttacgagtta cagcccctgg
     4381 catctcacag ctgcacttcc tcggagaaga ctacgtacga agagccacac acctgctccg
     4441 aatttttcaa cggccaagca aagaatttaa ggatgcctgt gcctgcagcc tacagcagcg
     4501 aactcaccaa aagccccagc agtgagccag gctcagcctt gctgcagtcc tgtctggagc
     4561 aggacaccgt gtgccatttc tctctcaatc cgagatgtaa ctgccgagac gcctacacac
     4621 acttacagta cggattacca gaaatccact gccagcagat gggtgactcc ttatgccaca
     4681 agtgtgcctc cactgcaggc ggctttgtcc agattcagag ttcggtggca cctctgaagg
     4741 cctcacacca agccgccgag ggccttctgc accctgccca gcacatgctg cccccaggga
     4801 tgcagaattc tcggcctagg aatttctttc tccactcagt gcagcacttt caggcccaag
     4861 aaaacctggg tcgcaccagc acacacagca cggatgagcg tcttcccagg acagcagagc
     4921 tgtcaccgcc accgtctgac agcaggagca ctgagtcttt ccaaagagct tgttgccatc
     4981 ctgagaataa ccaaaggaga ctctgcaaaa gtagagaccc aggggacaca gagggcagtg
     5041 gagggacaaa atccaaggtc tctggtttac caaaccagac tgacaaggag gagaagcaag
     5101 tggagccaag cctgctgcag accgatgaaa ctgtgaactc agaacattta aatcataacg
     5161 aatcaaactt tacattcagc catttaccag gggaggctgg ctgcaggtcc tgcccaaaca
     5221 gtccacagag ttgtagaagc atcatgagat cgaagtgcgg gactgaggac tgccagacgc
     5281 caaaccttga agccaatgtg cctgctgtac caacacactc agacctgtct ggcgagagtc
     5341 tgttaataaa aacactactc gagtacccct acgatgtgcc cgattatgca tacccatacg
     5401 atgttccaga ttacgctgtt taatctagag ggcccgttta aacccgctga tcagcctcga
     5461 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5521 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5581 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5641 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5701 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5761 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5821 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5881 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5941 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6001 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6061 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6121 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     6181 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6241 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6301 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6361 cctaactcca cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6421 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6481 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     6541 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     6601 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     6661 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     6721 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     6781 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6841 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6901 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6961 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     7021 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     7081 cgccaggttc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     7141 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     7201 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     7261 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     7321 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     7381 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     7441 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     7501 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     7561 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7621 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     7681 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7741 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7801 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7861 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7921 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7981 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8041 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8101 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8161 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8221 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8281 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8341 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8401 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8461 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8521 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8581 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8641 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8701 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8761 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8821 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8881 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8941 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9001 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9061 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9121 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9181 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9241 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9301 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9361 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9421 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9481 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9541 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9601 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9661 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9721 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9781 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9841 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     9901 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     9961 tgttggaggt cgctgagtag tgcgcaagca aaatttaagc tacaacaagg caaggcttga
    10021 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
    10081 gggccagata tacgcgtt
//
