LOCUS       sequence-246848-       16501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a MCS 
            for cloning gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16501)
  AUTHORS   Worthen JM, Yamburenko MV, Lim J, Nimchuk ZL, Kieber JJ, Schaller GE
  TITLE     Type-B response regulators of rice play key roles in growth, 
            development, and cytokinin signaling.
  JOURNAL   Development. 2019 Jun 3. pii: dev.174870. doi: 10.1242/dev.174870.
  PUBMED    31160418
REFERENCE   2  (bases 1 to 16501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2019 Jun 3. pii: dev.174870. doi: 10.1242/dev.174870."
COMMENT     Sequence Label: pARS3_MUbCAS9_MC
FEATURES             Location/Qualifiers
     source          1..16501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    99..123
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     143..165
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3699..3723
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3742..3797
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             3805..7908
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3805..7908
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             5591..5599
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7909..7935
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(8202..8226)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      8245..8952
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(8985..9003)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8986..9003)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        9098..10806
                     /gene="RUBQ2"
                     /label=Ubi promoter
                     /note="promoter for the rice (Oryza sativa) polyubiquitin 
                     gene RUBQ2"
     intron          9845..10806
                     /note="intron upstream of the coding sequence for the rice 
                     polyubiquitin gene RUBQ2"
     polyA_signal    13213..13387
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13500..13524
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      13602..14233
                     /label=oriV
                     /note="incP origin of replication"
     primer_bind     complement(15627..15644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15798..16386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15878..15897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta
       61 aatatccgat tattctaata aacgctcttt tctcttaggt ttacccgcca atatatcctg
      121 tcaaacactg atagtttcgc gtagcggata acaatttcac acagggatat cactagtaaa
      181 aggtacattt aaatcagttc gatgacccta ggctgaactg gtacggccgg cctcgatgct
      241 tacgcacgtg aattccggat cgcctgcagg gacttgacaa ttgtaccagt ttaaacaagt
      301 tgtaatgagt tgctggcctc tcttgggatt gttcatcaaa gtagctgtcc ctacacacca
      361 caacagaatg gtgttgtgga gagaaagcac agacacatcc ttgagatggc aagggcactt
      421 aagtttcaga gtggtgtacc taccaggttt tggggagact gtgtcaagac tgttgtatac
      481 ctgatcaata gactccctac tccaatctta caaggcaaat gtccatatga actactctat
      541 cagaaacatg ccaagcttga tcacttgaga gtgtttgggt gtcttgcttt tgcagcaaca
      601 ctgcccaaag gtgacaaact tgcacctaga gccaagcgaa ccatcttcat tggttattct
      661 gagactcaaa agggttacag gttgtatgac ttggataata aggtgatcat agtgagcagg
      721 gatgtagttt tcagagagtt tcagttccct ttcaaagagt ccatctccca tgaacctgat
      781 atgttcactc gagcaggttc acttagctct gaagatacag ctttgcagct agtgtataat
      841 gatatttttc cttccagctt tcatactgat gatgatgata ctccagttct tattcctgac
      901 attgtagagg ccaatgagga tatcactttg gggactcatt ctacccaaaa tcatatcact
      961 tcagctgatg ctgcaccttc aggccacctg catgctcctg cagagtcagt tgagtcagct
     1021 gagcctgacc tagcaaatgc tgaatttgag caccatactg ctgtagctga tccctctcta
     1081 gttccagcaa acctgcacac tagacctaaa cgcaatgcag gtcctcccat ctggctcaag
     1141 gactttgtga cactgaacaa aggctctagg gatgttccat accctatagc caactatgtt
     1201 tcctatgatc acttgcctgt tcactatcaa gcttatttga gtgctttctc cacttatact
     1261 gaacctaaat ccttcaaaga agcagctcaa gatgagaaat ggatggaggc catgtccctt
     1321 gagatacagg ctcttgagga taataacacc tgggagattg tccctttacc ccctggtaaa
     1381 cagcctatag ggtccaaatg ggtgtacaaa attaaataca aagctaatgg tgaagttgac
     1441 aggtttaagg caaggctagt ggccaaggga tacactcagc aagaaggcct tgactaccat
     1501 gaaacttttt ctccagtggc caaaatggtc actgtaaaag ctgtcatatc tgttgctgct
     1561 tccaagggct ggttcctttt ccagatggat gtcaacaatg cttttctaca aggtgacctc
     1621 atggaagaag tttatatgtc tctgcctcag ggttttcaca gccaagggga gaccaaagtg
     1681 tgcaggctgt tgaatccctc tatggtttaa agcaagcatc aaggcagtgg aacatcaagc
     1741 ttaccactgt ccttatgcag gctggtttta tgcaaagtgc ttatgatcac tccttgttta
     1801 ccaaaagaga ggggactgac cttgtcataa tcctgattta tgtggatgat ctgttaataa
     1861 caggcagcag caatgttttg atttcagaag caaaggcaac cctgcatcag catttcaaga
     1921 tgaaagattt gggagaacta aaatacttct taggcattga ggtgctgaga tcagaaaagg
     1981 gaatcctact gaaccagagg aagtatgcac tggaactgat atcaggtgtt ggtctgggag
     2041 gctgcagacc agtgtcaacc ccaatggaac agaaccaaag gctgactact gtagaatatg
     2101 acaagcactt aggaaagact gatgatgcag agttagagga tgtgggatcc tatcagagac
     2161 tggtgggaaa gcttctctac ttgacaatca caaggccaga tatcagcttt gcagtgcagg
     2221 tgctgagtca attcatgcag caacccaaac agtcacacct ggaagcagca ttgagggttg
     2281 tgaagtacat aaaaggttct ccaggtgtta ggaagcggcc gctgcagcgt cgcgtcgggc
     2341 caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc
     2401 gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga
     2461 cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga
     2521 ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc
     2581 ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc
     2641 tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc
     2701 ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta
     2761 cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt
     2821 acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt
     2881 tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt
     2941 tgtttcgttg catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact
     3001 tgtttgtcgg gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt
     3061 tgggcggtcg ttctagatcg gagtagaaat ctgtttcaaa ctacctggtg gatttattaa
     3121 ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag atgatggatg
     3181 gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg catatacaga
     3241 gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt
     3301 ctagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt ttggaactgt
     3361 atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat atcgatctag
     3421 gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca
     3481 tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata
     3541 attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt
     3601 ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac
     3661 cctgttgttt ggtgttactt ctgcagggcg cgccatcaac aagtttgtac aaaaaagcag
     3721 gctctttaaa gtatttttac aacaattacc aacaacaaca aacaacaaac aacattacaa
     3781 ttactattta caattacaaa aaaaatggac aaaaaataca gcattgggtt ggacattggg
     3841 acaaatagcg tgggttgggc agtcattaca gacgaataca aggttcctag caagaagttc
     3901 aaggtgttgg gcaacactga caggcactcg atcaagaaaa atctcattgg tgcactcctt
     3961 ttcgatagtg gggagactgc cgaagcgacc agactgaaaa ggactgctag aaggcgctat
     4021 acccggcgta agaatcgcat ttgctacctc caggagatat tctctaacga aatggccaag
     4081 gttgatgaca gcttctttca tcgcttggag gaatcattcc tcgtcgagga agacaagaaa
     4141 cacgagcggc atccgatctt tggaaacatt gttgatgagg tcgcatatca cgaaaagtac
     4201 cccacgatct accatcttcg caagaaactg gtggattcaa ctgacaaggc tgatctgcgg
     4261 ttgatatacc tcgcacttgc tcacatgatc aagttccgtg gccattttct tattgaggga
     4321 gacctgaacc ctgataattc tgacgtcgat aagttgttca tccagctcgt gcaaacatac
     4381 aatcagctct ttgaggaaaa ccctatcaac gcctctggag ttgatgccaa agcgatcctt
     4441 tccgcgaggc tgtcgaagag tagaaggctg gagaacttga tcgcccaact tcctggcgaa
     4501 aagaaaaacg gtttgttcgg gaatctcatt gcgctgagct tgggactcac gccaaacttc
     4561 aagtctaact tcgacctcgc agaggatgct aaacttcagc tgtccaagga tacttatgat
     4621 gacgatcttg acaacctgtt ggcacaaatc ggtgaccagt acgctgattt gttcctcgct
     4681 gccaagaacc tcagcgatgc catactcctt tcagacatcc tccgcgtgaa cactgagatt
     4741 accaaagcac ctctgtctgc ttccatgata aagcgttatg atgagcacca tcaagacctc
     4801 actctgttga aggcgttggt tagacagcaa ctcccagaga agtacaaaga aatcttcttt
     4861 gaccaatcta agaatggcta cgccggatat attgatggcg gagcgtccca ggaggagttc
     4921 tacaagttta tcaaacctat tcttgagaag atggatggta cggaggaact ccttgttaaa
     4981 ctgaatagag aggatctctt gcgcaagcaa cggactttcg acaacggcag cataccgcac
     5041 cagatccatc tcggagagct tcacgccatc cttcgccggc aggaagattt ctaccccttt
     5101 ctgaaggaca accgtgagaa gatcgaaaaa atccttactt tcagaatccc ttactatgtc
     5161 ggtccactgg ctagaggcaa ctctagattt gcttggatga cacgcaagag tgaggaaact
     5221 atcacccctt ggaacttcga ggaagtggtt gacaagggcg catcggctca aagtttcatt
     5281 gagagaatga ctaattttga taagaacctt ccgaacgaga aagtcctgcc caagcattca
     5341 ctcctttacg agtatttcac agtgtataac gaacttacga aggtgaaata cgttactgag
     5401 ggtatgcgga agccggcatt cctgtcgggg gaacaaaaga aagctatagt ggatctgttg
     5461 ttcaaaacta acaggaaggt taccgtcaag cagcttaagg aggattactt caagaaaata
     5521 gaatgttttg actcggtgga gatcagtggc gttgaagata gattcaacgc ttcactggga
     5581 acctaccacg acctccttaa gatcattaag gacaaagatt tcttggataa cgaggaaaat
     5641 gaggacattc tcgaagatat agtccttaca ctgacgttgt ttgaggaccg tgaaatgatc
     5701 gaggaaagat tgaagaccta tgctcatctc ttcgacgata aggttatgaa gcagctcaag
     5761 cgtagaaggt acacaggttg ggggcgtctt tctagaaagc tgattaatgg cataagggat
     5821 aaacaatctg gaaagacgat tctggatttc ctcaagtccg acggcttcgc caaccgcaat
     5881 tttatgcagc ttatacacga cgattccctg actttcaaag aggacatcca gaaggcccaa
     5941 gttagcggcc aaggagattc acttcacgag catatcgcca acctggcggg tagcccggcg
     6001 atcaagaaag ggattcttca gaccgtcaag gtcgtggatg agctggtgaa agttatgggc
     6061 cggcataagc ccgaaaacat agtgatcgag atggcccgtg aaaatcagac tacccaaaaa
     6121 ggacagaaga actcccgcga gcggatgaaa aggatagagg aaggcatcaa ggaacttgga
     6181 agccagatcc tgaaggagca cccagttgaa aacacacagt tgcaaaatga gaagctctat
     6241 ctttactatc tccaaaatgg tagagacatg tatgtggatc aggagttgga cattaacagg
     6301 ctctcagact acgatgtcga ccatatcgtg ccgcaatcgt tccttaagga cgatagtata
     6361 gataataagg tgctgactcg gtcggataaa aaccgtggca agtcggacaa tgttcccagt
     6421 gaggaagttg tgaagaagat gaagaactac tggcgccaac tgttgaatgc aaaattgatc
     6481 acccagagga agtttgacaa cctcacaaaa gcagagcgcg gtgggctcag tgaacttgat
     6541 aaagctggat tcattaagag gcaacttgtt gagacacgcc agattacgaa gcacgtcgcg
     6601 cagatactgg attctaggat gaacaccaag tacgacgaga atgataaatt gatcagagaa
     6661 gtgaaggtta tcacattgaa gtctaaactc gtgtccgatt ttcgcaaaga cttccaattt
     6721 tataaggttc gggagatcaa caattatcac catgcacatg atgcttacct caacgccgtg
     6781 gttggtacgg cgttgattaa gaaatacccg aagctggagt ctgagttcgt gtacggggat
     6841 tataaggtct acgacgtgag gaaaatgatc gctaagagtg agcaggaaat tggtaaagcc
     6901 accgcgaagt atttctttta ctctaacatc atgaacttct tcaagacaga gataacgttg
     6961 gcaaatggtg aaatacgtaa gagacctctc atcgagacta acggtgaaac cggggaaatc
     7021 gtttgggata aagggcggga ctttgctact gttcgtaagg tcctctccat gccgcaagtg
     7081 aatattgtta agaaaacaga ggtccagacg ggcggattct ctaaggaatc catccttccc
     7141 aaaagaaaca gcgacaagct gattgcgagg aagaaagatt gggaccctaa gaaatatggt
     7201 gggttcgatt ctccaaccgt tgcctactcc gtcttggtcg tggcgaaggt tgagaagggt
     7261 aaatctaaga aactcaaatc cgtcaaggaa ctccttggga ttactatcat ggagaggtct
     7321 tccttcgaaa agaatcctat cgatttcctt gaggctaaag gctataagga agtgaagaag
     7381 gatctcatca tcaagctccc aaagtacagc ctgtttgagt tggaaaacgg aaggaagcgc
     7441 atgttggcat cagctggaga gctccagaaa gggaacgagc tggccttgcc ttctaagtat
     7501 gtgaacttcc tctatcttgc gtcgcactac gagaagctga aaggcagtcc agaggacaac
     7561 gaacagaaac aactttttgt tgagcaacac aagcattatc tggatgagat tatagaacag
     7621 atcagcgagt tctcaaaaag agtcattctt gccgatgcta acctcgacaa ggtgctctcc
     7681 gcatacaaca aacacagaga caagccgatc agggagcagg ctgaaaatat cattcatctc
     7741 ttcactctta ccaacctggg tgcccccgcg gcattcaagt attttgatac aacgattgac
     7801 cggaaacgtt acacaagcac gaaggaggtc cttgatgcga ccctgattca tcaaagcata
     7861 acagggctct atgagacgag aatcgatttg tcacagctcg gcggagacta cccgtatgac
     7921 gtgcccgatt acgctgccgc ggcagctgag ttcaagcgcg aggaacaggc ccggaaggcg
     7981 aaagtgaaca atgagaagaa aacagaaatc gttaaacctg agagctgctc aaacgaggga
     8041 gacgttaagg atcttaagag gaaagactct gaggatggca acgaaggaga ggaagaggaa
     8101 gctagctcaa aacctaagaa accaaaggtg gcactctcac atctccaaga cattgacgac
     8161 accgaagcag accaagaaga agaataactc gagatatcta gacccagctt tcttgtacaa
     8221 agtggttgat gatcctctag agtcctgctt taatgagata tgcgagacgc ctatgatcgc
     8281 atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa acctgagcat gtgtagctca
     8341 gatccttacc gccggtttcg gttcattcta atgaatatat cacccgttac tatcgtattt
     8401 ttatgaataa tattctccgt tcaatttact gattgtaccc tactacttat atgtacaata
     8461 ttaaaatgaa aacaatatat tgtgctgaat aggtttatag cgacatctat gatagagcgc
     8521 cacaataaca aacaattgcg ttttattatt acaaatccaa ttttaaaaaa agcggcagaa
     8581 ccggtcaaac ctaaaagact gattacataa atcttattca aatttcaaaa gtgccccagg
     8641 ggctagtatc tacgacacac cgagcggcga actaataacg ctcactgaag ggaactccgg
     8701 ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga gtattggccg tccgctctac
     8761 cgaaagttac gggcaccatt caacccggtc cagcacggcg gccgggtaac cgacttgctg
     8821 ccccgagaat tatgcagcat ttttttggtg tatgtgggcc ccaaatgaag tgcaggtcaa
     8881 accttgacag tgacgacaaa tcgttgggcg ggtccagggc gaattttgcg acaacatgtc
     8941 gaggctcagc aggaccggca tgcaagctag cttactagtg atattctata gtgtcaccta
     9001 aatctgcgcg gccgcaaagt cgacgaattc tcccgggtgg atccaaggcc ttctagaaaa
     9061 actcgagaac gttgggcccc aattggctag caagcttatt cgggtcaagg ggaagccagc
     9121 gcgccacccc acgtcagcaa atacggaggc gcggggttga cggcgtcacc cggtcctaac
     9181 ggcgaccaac aaaccagcca gaagaaatta cagtaaaaaa aaagtaaatt gcactttgat
     9241 ccacctttta ttacctaagt ctcaatttgg atcaccctta aacctatctt ttcaatttgg
     9301 gccgggttgt ggtttggact accatgaaca acttttcgtc atgtctaact tccctttcag
     9361 caaacatatg aaccatatat agaggagatc ggccgtatac tagagctgat gtgtttaagg
     9421 tcgttgattg cacgagaaaa aaaaatccaa atcgcaacaa tagcaaattt atctggttca
     9481 aagtgaaaag atatgtttaa aggtagtcca aagtaaaact tatagataat aaaatgtggt
     9541 ccaaagcgta attcactcaa aaaaaatcaa cgagacgtgt accaaacgga gacaaacggc
     9601 atcttctcga aatttcccaa ccgctcgctc gcccgcctcg tcttcccgga aaccgcggtg
     9661 gtttcagcgt ggcggattct ccaagcagac ggagacgtca cggcacggga ctcctcccac
     9721 cacccaaccg ccataaatac cagccccctc atctcctctc ctcgcatcag ctccaccccc
     9781 gaaaaatttc tccccaatct cgcgaggctc tcgtcgtcga atcgaatcct ctcgcgtcct
     9841 caaggtacgc tgcttctcct ctcctcgctt cgtttcgatt cgatttcgga cgggtgaggt
     9901 tgttttgttg ctagatccga ttggtggtta gggttgtcga tgtgattatc gtgagatgtt
     9961 taggggttgt agatctgatg gttgtgattt gggcacggtt ggttcgatag gtggaatcgt
    10021 ggttaggttt tgggattgga tgttggttct gatgattggg gggaattttt acggttagat
    10081 gaattgttgg atgattcgat tggggaaatc ggtgtagatc tgttggggaa ttgtggaact
    10141 agtcatgcct gagtgattgg tgcgatttgt agcgtgttcc atcttgtagg ccttgttgcg
    10201 agcatgttca gatctactgt tccgctcttg attgagttat tggtgccttg ggttggtgca
    10261 aacacaggct ttaatatgtt atatctgttt tgtgtttgat gtagatctgt agggtagttc
    10321 ttcttagaca tggttcaatt atgtagcttg tgcgtttcga tttgatttca tatgttcaca
    10381 gattagataa tgatgaactc ttttaattaa ttgtcaatgg taaataggaa gtcttgtcgc
    10441 tatatctgtc ataatgatct catgttacta tctgccagta atttatgcta agaactatat
    10501 tagaatatca tgttacaatc tgtagtaata tcatgttaca atctgtagtt catctatata
    10561 atctattgtg gtaatttctt tttactatct gtgtgaagat tattgccact agttcattct
    10621 acttatttct gaagttcagg atacgtgtgc tgttactacc tatctgaata catgtgtgat
    10681 gtgcctgtta ctatcttttt gaatacatgt atgttctgtt ggaatatgtt tgctgtttga
    10741 tccgttgttg tgtccttaat cttgtgctag ttcttaccct atctgtttgg tgattatttc
    10801 ttgcagatgc agatctttgt gaagacattg accggcaaga ctatcaccct ggaggtggag
    10861 tcctctgaca ccatcgacaa tgtcaaggct aagatccaag ataaggaggg catccccccg
    10921 gaccagcagc gtctcatctt cgctggcaag cagctcgaag atggcaggac ccttgctgac
    10981 tacaacatcc agaaggagtc cacccttcac cttgtcctcc gcctccgtgg tggcggatcc
    11041 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
    11101 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
    11161 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
    11221 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
    11281 ggggagttta gcgagagcct gacctattgc atctcccgcc gttcacaggg tgtcacgttg
    11341 caagacctgc ctgaaaccga actgcccgct gttctacaac cggtcgcgga ggctatggat
    11401 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
    11461 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
    11521 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
    11581 ctgagagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
    11641 ctatacgaat agcttagaca gccgcttagc cgaattggaa agacggaaaa gcccgaagag
    11701 gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa
    11761 gtaagtggct tttcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
    11821 taaaatatta tattttactg gatgaattgt tttagtacct agatgtggcg caacgatgcc
    11881 ggcgacaagc aggagcgcac cgacttcttc cgcatcaagt gttttggctc tcaggccgag
    11941 gcccacggca agtatttggg caaggggtcg ctggtattcg tgcagggcaa gattcggaat
    12001 accaagtacg agaaggacgg ccagacggtc tacgggaccg acttcattgc cgataaggtg
    12061 gattatctgg acaccaaggc accaggcggg tcaaatcagg aataagggca cattgccccg
    12121 gcgtgagtcg gggcaatccc gcaaggagca ccgtcatgcg tgcgccccgc gaaaccttcc
    12181 agtccgtcgg ctcgatggtc cagcaagcta cggccaagat cgagcgcgac agcgtgcaac
    12241 tgacacgcga ggaactatga cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa
    12301 acaggtcagc gaggccaagc aagccgcgtt gctgaacgct ctgccctgtt caccacgcgc
    12361 aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt caacaaggac
    12421 gtgaagatca caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg
    12481 gccggtatta cacgaaggcc gaggaatgcc tgtcgcgcct acagcgtccc gttgccaggt
    12541 cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac cactacacga aattcatatg
    12601 ggagaagtac cgcaagctga ttccacccgc gtgaagaagt ggcgcgagca ggtcggcgaa
    12661 gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa tgtgctttgg
    12721 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
    12781 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
    12841 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
    12901 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccacg actccgggcg
    12961 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
    13021 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
    13081 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
    13141 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagaa atagttaatc
    13201 gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg gaattagggt
    13261 tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
    13321 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat
    13381 ccagatcccc cgaattaatt cggcgttaat tcagtacatt aaaaacgcgt acggttaaaa
    13441 ccaccccagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
    13501 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
    13561 acaaaatcac cactcgatac aggcagccca tcagtccact agacgctcac cgggctggtt
    13621 gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg
    13681 aagccgtgtg cgagacaccg cagccgccgg cgttgtggat acctcgcgga aaacttggcc
    13741 ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg
    13801 ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa
    13861 atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg
    13921 ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga
    13981 tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag
    14041 gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac
    14101 cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag
    14161 ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc
    14221 ctcccggccc gctctcgcgt tggcagcatc acccataatt gtggtttcaa aatcggctcc
    14281 gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat
    14341 ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct
    14401 tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa
    14461 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
    14521 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
    14581 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
    14641 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
    14701 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
    14761 aaagccctga aaagattatc attacttact gaataacgat ctggccgatg tggattgcga
    14821 aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttattgat
    14881 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
    14941 agggaggata ttggggaaga acagtatggg tgaatgaatc ggacgtttga ccggaaggca
    15001 tacaggcaag aactgatcga cgcggggttt tccgccgagg atgccgaaac catcgcaagc
    15061 cggctccccc tgccctgccc gcgccatcgg ccgccgtgga gcgttcgcgt cgtctcgaac
    15121 aggaggcggc aggtttggcg aagtcgatga ccatcgacac acgaagcagc agatcaagga
    15181 aatgcagctt tccttgttcg atattgcgcc gtggccggac acgatgcgag cgatgccaaa
    15241 cgacacggcc cctacaccgg cgtcgagctg cgggccgacg atgacgaact ggtgtggcag
    15301 caggtgttgg agtacgcgaa gcgcacccct atcggcgagc cgatgcgacg gcgatgggct
    15361 tcacgtccga ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc
    15421 tggaccgtgg caagaaaagt cgccgacggc ccgacggatg ttcgactatt tcagctcgca
    15481 ccgggagccg tacccgctca agctggaaac cttccgcctc atgtgcggat cgatgacctg
    15541 gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc agcagccagc
    15601 gctttactga gatcctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    15661 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    15721 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    15781 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    15841 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    15901 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    15961 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    16021 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    16081 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    16141 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    16201 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    16261 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    16321 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    16381 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    16441 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttggat
    16501 c
//
