LOCUS       sequence-246857-       12179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of wild type dDispatched HA in Drosophila..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12179)
  AUTHORS   Stewart DP, Marada S, Bodeen WJ, Truong A, Sakurada SM, Pandit T, 
            Pruett-Miller SM, Ogden SK
  TITLE     Cleavage activates dispatched for Sonic Hedgehog ligand release.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 31678. doi: 10.7554/eLife.31678.
  PUBMED    29359685
REFERENCE   2  (bases 1 to 12179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 31678. doi"; pages: " 
            10.7554/eLife.31678"
COMMENT     Sequence Label: pUAS attB V5 dDispatched WT HA
FEATURES             Location/Qualifiers
     source          1..12179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..34
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    67..161
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        180..418
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     365..386
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             762..803
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4149..4175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          4262..4327
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4457..4477
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4749..4883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4786..4805)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4840..4859
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4912..4981
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(5200..5218)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5239..5255)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5239..5255)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5252..5274)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5263..5279
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5287..5293,5294..5311,5312..5317))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5332..5353
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5470..5487)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5641..6229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5721..5740)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6400..7191,7192..7260))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7023..7042
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7261..7365)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7391..7846)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7528..7549)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7740..7759
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7973..7995
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7987..8004
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7988..8004
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8011..8030
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8011..8029
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8063..8081)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(8533..8604,9006..9279,9354..10008,10070..10385,
                     10589..10720,10791..11405)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 ataacttcgt ataatgtatg ctatacgaag ttatgctagc ggatccaagc ttgcatgcct
       61 gcaggtcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
      121 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgagcgc
      181 cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt
      241 gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa
      301 tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc
      361 aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa
      421 tagggaattg ggaattcatg ttgtgcttcg actcggagag gatgaactgg tactaccacg
      481 tcctggccag gcgtccctac ctggtggtcg tctccattgc tgtctactgt gtcgcttgca
      541 tcatcgtggc cctcgtcctg aacaagctgc cggacttcag tgatcccacc ttgggcttcg
      601 aaacccgcgg caccaaaatt ggagaacgac tgacggcctg gtacaacctg ctgcaagaga
      661 ctgaccacca tggtgctctt ttctcaaatc catcggatct gtgggagagg agacgggtag
      721 agcagggcta tgtggagacg aagctgcatc cgaatcacgt gggtaagcct atccctaacc
      781 ctctcctcgg tctcgattct acgcacgtga ggcgacgcaa aaacaagcac aagaacagaa
      841 acaagaacaa gcgacgcaag gaacaaaatc aaagcagcca cgagcatcac gatgtggcgc
      901 agaagatgat gcagttcaaa aaacggctga aggctactag ttctccctct ccaaatctcg
      961 gctttgacac ctggatcggg gacagtggag tatttcgcga ctacgagatt acaaacgaca
     1021 gcgcttcctc ctctttggaa cccacgcgac gcactgagca aatcgaatat ggccacaaca
     1081 ccacctcagt ggacgaggaa gagcatcagc agcgggtgca gaccaagaag agcacttggc
     1141 ggctcctaaa gcaagccgcc accttgccca ccgacggttg ggcagacatg catcgtcgcc
     1201 aacccatcga gggattcttt tgcgattcat cgccccggaa ggagtactct cattttgtgg
     1261 tccagcggat cggtcccaac gccaccgatt ctctatttga tttgaacgga ctgctggcca
     1321 tgtgtcagtt gcaggatcag attaccgaag tccccagcta ccgggcattt tgcgagccgg
     1381 agatgcttac caccgagtgc tgccggccat ggtcactgcc caactacgcc gccatgctgg
     1441 ccaacaaaag ttcctgtttc gaccttacca cggaggatgt tacttcgctg cacacgctgc
     1501 tcttgggctg ctatgagtac ttccatgacc taaagatgga caaccactgc aacgaaatac
     1561 cgcattgtcg tgctcctgaa gagtgcaagc ggctaaatat cgtctttaac gtcctgaatt
     1621 ttctcaccga ctttagtttc atcaagtcta atgactcgaa tgtatacctg aaatacgcca
     1681 tgatttttat acccgtggcg caatccaacc gcttgctgcc actgttccac gaatgggaag
     1741 acgtggagct gatcaacgag cttgtggaag tggttgcgat ggacttgggc ctcgaaaacg
     1801 aactctttaa cgagctgctg ctgacggacg tgtggctagt ttcgctggga gggacattcg
     1861 tcatggctag cgtgtggttg tacacgggat cggcattcat caccttgatg tcctgcgtag
     1921 ccatatgctt ttcgctagga ctagcgtact ttttttacgc catcgtgctg gagtttgagt
     1981 tcttccccta tatgaacctt ctggccgtgg tagtaataat tggcattgga gcagacgacg
     2041 tatttctgtt tcttaagatc tggcactgtg tgctgaccga gaggttcagc aatcgatgta
     2101 ccttgaccac tcagtcccag agtgctcttc ccaccctgga gaacagtgat cacacggagt
     2161 cactggaaaa cataatggcg ctgactatgc gacatgccgc tgcctccatg ttcgttacct
     2221 cacttaccac cgccggcgcc ttttatgcct cctacagcag ctctataaca gctataaagt
     2281 gctttgggat ttttgcggga actgtcgtgg tgaccaacta cttactaatg ataacttggt
     2341 tgcctgcatc ggtctccatc atggaacgac tatttgccac aaggatgtcc tgccatcatc
     2401 cgatgtcaat aaagctgatc cacgcctgca agaagtcaat taaccgattt tgtcagatgt
     2461 ttgaggagtg catcacgaaa agcatcatga actatgccta tctctggctg ctgatctttg
     2521 gggctctagg cgcgtccagt gccgtcattg tgttctggta tccaggactt cagttgccgg
     2581 aaaaatcaca cttccagctc tttgtgtcaa agcatccatt tgaggtttac tccagtctca
     2641 agcagcagtt ctggttcgag aaaccattgc aggcgtacga gaacttcaag atgcacatgc
     2701 atttcgtctg gggcgttcaa gcggtggacg acggcgacta tacgaacccc aactcatacg
     2761 gccacctgca ctatgacaat aattttaacg tatccagcag gccggcacaa ctctggatcc
     2821 ttgatttttg ccagagtgtg cgccagcaac ccttttacaa agagactctc ggcatgctgt
     2881 tgcccaattg tttcattgaa aatcttatcg actatatgaa gcgcagatgc atcgatgata
     2941 tggacagtac caggaaagac cgttcaccct gctgtgacgc acagtttccc tttgagccgc
     3001 acatatttga gtactgcttg ccacaaagca tatccaacat gtatgacact acatttttcc
     3061 ggcccggcgt ggcaggaccc aagtttgcag aggctccccg actggagact gaggattacc
     3121 taggaatgag tggaaatgag agtgcagagt acagcaccaa cggatcattt acaccgctac
     3181 tagtcaaagc cctggtcatc gagttcgagt ccaacgtggc ctacagcacc atctacgcaa
     3241 atattaggca gttctacgag tctgtagagc actggtttca gatgcagtta aaaacggcac
     3301 cgccggaact tcaaggagga tggttcacga gcgacctgaa attttacaat gtgcaggaca
     3361 ccctttcgca cgacaccttt gttgccattt gtctggccat ggctgcatca cttgcagtgc
     3421 tcctatgctt caccgtaaac atattgattt ccatctacgc cgtactaacc gtgtcgctaa
     3481 gtatttttaa caccgtggct gtgctcatcc tgctcggctg gcagcttaac atcttggaga
     3541 gcattgcggt gagcacggct atcggtctgg ctgtggactt tagcctacat tacggtattc
     3601 actaccggat gtccccggtt aaggagagat tggcagccac acagtttgta ctatcccgca
     3661 tcattggacc cacagtgatg gcggccacca caactggtct agctggcgga atcatgatgg
     3721 catccaacat attgccctac atacagattg gcgtcttcct ggtcgttgtc atgatcgtta
     3781 gctggttcta tgccactttc tttctaatga gtctgcttcg ggttgccggt cctcagcatg
     3841 gttttctgga actcaagtgg ccgttgtgga gcaagcgaag cagtggcagc agcaagttct
     3901 acgagcggaa acccagccaa gtgatcgcca gcgagcagct gctgaccccc acaagctcgg
     3961 ccatcgttga gttggcgaac tcggagacgc acgaactgga gtccctaaac tccaacagcc
     4021 tgatcaaaac catttcgggc atcgaaagcg cccacgcatt gtcctcgctg ccgagggact
     4081 tcgagcactc attccagacg atgcacgagt gcaaatatca aacgtatccg tctacatcca
     4141 atggtaccta cccatacgac gtcccagact acgcttgatc tagaggatct ttgtgaagga
     4201 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     4261 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     4321 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     4381 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     4441 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     4501 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     4561 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattt
     4621 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     4681 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     4741 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4801 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4861 atgtatctta tcatgtctgg atccactagt gtcgacgatg taggtcacgg tctcgaagcc
     4921 gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat
     4981 ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg
     5041 caccgggaag ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg
     5101 tgcgacggcg tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac
     5161 ggcgggcatg tcgacactag ttctagccag cttttgttcc ctttagtgag ggttaatttc
     5221 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5281 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5341 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5401 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5461 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5521 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5581 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5641 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5701 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5761 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5821 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5881 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5941 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6001 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6061 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6121 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6181 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6241 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6301 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6361 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6421 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6481 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6541 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6601 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6661 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6721 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6781 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6841 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6901 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6961 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7021 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7081 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7141 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7201 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7261 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7321 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7381 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     7441 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     7501 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     7561 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     7621 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     7681 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     7741 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     7801 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     7861 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     7921 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     7981 cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt
     8041 acgtaccggg cccctagtat gtatgtaagt taataaaacc catttttgcg gaaagtagat
     8101 aaaaaaaaca tttttttttt ttactgcact ggatatcatt gaacttatct gatcagtttt
     8161 aaatttactt cgatccaagg gtatttgatg taccaggttc tttcgattac ctctcactca
     8221 aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg
     8281 ccgtctcttt cgccgctgcg tccgctatct ctttcgccac cgtttgtagc gttacgtagc
     8341 gtcaatgtcc gccttcagtt gcattttgtc agcggtttcg tgacgaagct ccaagcggtt
     8401 tacgccatca attaaacaca aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt
     8461 tgttttttga gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga
     8521 aaaatcccgg caatgggcca agaggatcag gagctattaa ttcgcggagg cagcaaacac
     8581 ccatctgccg agcatctgaa caatgtgagt agtacatgtg catacatctt aagttcactt
     8641 gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac
     8701 aaaaatccca aaccgcaatt gcacaaacaa atagtgacac gaaacagatt attctggtag
     8761 ctgttctcgc tatataagac aatttttgag atcatatcat gatcaagaca tctaaaggca
     8821 ttcattttcg actatattct tttttacaaa aaatataaca accagatatt ttaagctgat
     8881 cctagatgca caaaaaataa ataaaagtat aaacctactt cgtaggatac ttcggggtac
     8941 tttttgttcg gggttagatg agcataacgc ttgtagttga tatttgagat cccctatcat
     9001 tgcagggtga cagcggagcg gcttcgcaga gctgcattaa ccagggcttc gggcaggcca
     9061 aaaactacgg cacgctccgg ccacccagtc cgccggagga ctccggttca gggagcggcc
     9121 aactagccga gaacctcacc tatgcctggc acaatatgga catctttggg gcggtcaatc
     9181 agccgggctc cggatggcgg cagctggtca accggacacg cggactattc tgcaacgagc
     9241 gacacatacc ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg
     9301 gctgatctgt gtgaaatctt aataaagggt ccaattacca atttgaaact cagtttgcgg
     9361 cgtggcctat ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac
     9421 cctgctgaat gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat
     9481 gcgactgctc aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt
     9541 ccagcaggat gacctcttta tcggctccct aacggccagg gaacacctga ttttccaagc
     9601 catggtgcgg atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt
     9661 gatccaggag ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt
     9721 gaaaggtctg tccggcggag aaaggaagcg tctggcattc gcctccgagg ctctaaccga
     9781 tccgccgctt ctgatctgcg atgagcccac ctccggactg gactccttta ccgcccacag
     9841 cgtcgtccag gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca
     9901 tcagccgtct tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag
     9961 ggtagctttc ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg
    10021 tgtttattaa gggtatctag tattacataa catctcaact cctatccagc gtgggtgccc
    10081 agtgtcctac caactacaat ccggcggact tttacgtaca ggtgttggcc gttgtgcccg
    10141 gacgggagat cgagtcccgt gatcggatcg ccaagatatg cgacaatttt gccattagca
    10201 aagtagcccg ggatatggag cagttgttgg ccaccaaaaa tctggagaag ccactggagc
    10261 agccggagaa tgggtacacc tacaaggcca cctggttcat gcagttccgg gcggtcctgt
    10321 ggcgatcctg gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga cttattcaga
    10381 caacggtgag tggttccagt ggaaacaaat gatataacgc ttacaattct tggaaacaaa
    10441 ttcgctagat tttagataga attgcctgat tccacaccct tcttagtttt tttcaatgag
    10501 atgtatagtt tatagttttg cagaagataa ataaatttca tttaactcgc gaatattaat
    10561 gagatgcgag taacatttta atttgcagat ggttgccatc ttgattggcc tcatcttttt
    10621 gggccaacaa ctcacgcaag tgggtgtgat gaatatcaac ggagccatct tcctcttcct
    10681 gaccaacatg acctttcaaa acgtctttgc cacgataaat gtaagtcatg tttagaatac
    10741 atttgcattt caataattta ctaactttct aatgaatcga ttcgatttag gtgttcacct
    10801 cagagctgcc agtttttatg agggaggccc gaagtcgact ttatcgctgt gacacatact
    10861 ttctgggcaa aacgattgcc gaattgccgc tttttctcac agtgccactg gtcttcacgg
    10921 cgattgccta tccgatgatc ggactgcggg ccggagtgct gcacttcttc aactgcctgg
    10981 cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca
    11041 gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca ttcctgctct
    11101 ttggcggctt cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc
    11161 tctcatggtt ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc
    11221 cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc
    11281 tggagacgct taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc
    11341 tcatcgtgag cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg
    11401 agtagccgac atatatccga aataactgct tgtttttttt ttttaccatt attaccatcg
    11461 tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac
    11521 aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg
    11581 gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta
    11641 tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct
    11701 cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc
    11761 gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg
    11821 cgcgattcgg ttcgctcaaa tggttccgag tggttcattt cgtctcaata gaaattagta
    11881 ataaatattt gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag
    11941 ctatatttat tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg
    12001 agtgattagc gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat
    12061 gcatctcaaa aaaatggtgg gcataatagt gttgtttata tatatcaaaa ataacaacta
    12121 taataataag aatacattta atttagaaaa tgcttggatt tcactggaac taggctagc
//
