LOCUS       sequence-246861-       12080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of V5 dDispatched Delta CS HA in Drosophila cells lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Stewart DP, Marada S, Bodeen WJ, Truong A, Sakurada SM, Pandit T, 
            Pruett-Miller SM, Ogden SK
  TITLE     Cleavage activates dispatched for Sonic Hedgehog ligand release.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 31678. doi: 10.7554/eLife.31678.
  PUBMED    29359685
REFERENCE   2  (bases 1 to 12080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 31678. doi"; pages: " 
            10.7554/eLife.31678"
COMMENT     Sequence Label: pUAS-attB V5 dDispatched delta CS HA
FEATURES             Location/Qualifiers
     source          1..12080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..34
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    67..161
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        180..418
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     365..386
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             762..803
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4050..4076
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          4163..4228
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4358..4378
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4650..4784
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4687..4706)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4741..4760
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4813..4882
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(5101..5119)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5140..5156)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5140..5156)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5153..5175)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5164..5180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5188..5194,5195..5212,5213..5218))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5233..5254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5371..5388)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5542..6130)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5622..5641)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6301..7092,7093..7161))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6924..6943
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7162..7266)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7292..7747)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7429..7450)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7641..7660
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7874..7896
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7888..7905
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7889..7905
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7912..7931
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7912..7930
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7964..7982)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(8434..8505,8907..9180,9255..9909,9971..10286,
                     10490..10621,10692..11306)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 ataacttcgt ataatgtatg ctatacgaag ttatgctagc ggatccaagc ttgcatgcct
       61 gcaggtcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
      121 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgagcgc
      181 cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt
      241 gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa
      301 tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc
      361 aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa
      421 tagggaattg ggaattcatg ttgtgcttcg actcggagag gatgaactgg tactaccacg
      481 tcctggccag gcgtccctac ctggtggtcg tctccattgc tgtctactgt gtcgcttgca
      541 tcatcgtggc cctcgtcctg aacaagctgc cggacttcag tgatcccacc ttgggcttcg
      601 aaacccgcgg caccaaaatt ggagaacgac tgacggcctg gtacaacctg ctgcaagaga
      661 ctgaccacca tggtgctctt ttctcaaatc catcggatct gtgggagagg agacgggtag
      721 agcagggcta tgtggagacg aagctgcatc cgaatcacgt gggtaagcct atccctaacc
      781 ctctcctcgg tctcgattct acgcacgtga ggcgacgcaa aaacaagcac aagaacagaa
      841 acaagaacaa gcgacgcaag gaacaaaatc aaagcagcca cgagcatcac gatgtggcgc
      901 agaagatgat gcagttcaaa aaacggctga aggctactag ttctccctct ccaaatctcg
      961 gctttgacac ctggatcggg gacagtggag tatttcgcga ctacgagatt acaaacgaca
     1021 gcgcttcctc ctctttggaa cccacgcgac gcactgagca aatcgaatat ggccacaaca
     1081 ccacctcagt ggacgaggaa gagcccatcg agggattctt ttgcgattca tcgccccgga
     1141 aggagtactc tcattttgtg gtccagcgga tcggtcccaa cgccaccgat tctctatttg
     1201 atttgaacgg actgctggcc atgtgtcagt tgcaggatca gattaccgaa gtccccagct
     1261 accgggcatt ttgcgagccg gagatgctta ccaccgagtg ctgccggcca tggtcactgc
     1321 ccaactacgc cgccatgctg gccaacaaaa gttcctgttt cgaccttacc acggaggatg
     1381 ttacttcgct gcacacgctg ctcttgggct gctatgagta cttccatgac ctaaagatgg
     1441 acaaccactg caacgaaata ccgcattgtc gtgctcctga agagtgcaag cggctaaata
     1501 tcgtctttaa cgtcctgaat tttctcaccg actttagttt catcaagtct aatgactcga
     1561 atgtatacct gaaatacgcc atgattttta tacccgtggc gcaatccaac cgcttgctgc
     1621 cactgttcca cgaatgggaa gacgtggagc tgatcaacga gcttgtggaa gtggttgcga
     1681 tggacttggg cctcgaaaac gaactcttta acgagctgct gctgacggac gtgtggctag
     1741 tttcgctggg agggacattc gtcatggcta gcgtgtggtt gtacacggga tcggcattca
     1801 tcaccttgat gtcctgcgta gccatatgct tttcgctagg actagcgtac tttttttacg
     1861 ccatcgtgct ggagtttgag ttcttcccct atatgaacct tctggccgtg gtagtaataa
     1921 ttggcattgg agcagacgac gtatttctgt ttcttaagat ctggcactgt gtgctgaccg
     1981 agaggttcag caatcgatgt accttgacca ctcagtccca gagtgctctt cccaccctgg
     2041 agaacagtga tcacacggag tcactggaaa acataatggc gctgactatg cgacatgccg
     2101 ctgcctccat gttcgttacc tcacttacca ccgccggcgc cttttatgcc tcctacagca
     2161 gctctataac agctataaag tgctttggga tttttgcggg aactgtcgtg gtgaccaact
     2221 acttactaat gataacttgg ttgcctgcat cggtctccat catggaacga ctatttgcca
     2281 caaggatgtc ctgccatcat ccgatgtcaa taaagctgat ccacgcctgc aagaagtcaa
     2341 ttaaccgatt ttgtcagatg tttgaggagt gcatcacgaa aagcatcatg aactatgcct
     2401 atctctggct gctgatcttt ggggctctag gcgcgtccag tgccgtcatt gtgttctggt
     2461 atccaggact tcagttgccg gaaaaatcac acttccagct ctttgtgtca aagcatccat
     2521 ttgaggttta ctccagtctc aagcagcagt tctggttcga gaaaccattg caggcgtacg
     2581 agaacttcaa gatgcacatg catttcgtct ggggcgttca agcggtggac gacggcgact
     2641 atacgaaccc caactcatac ggccacctgc actatgacaa taattttaac gtatccagca
     2701 ggccggcaca actctggatc cttgattttt gccagagtgt gcgccagcaa cccttttaca
     2761 aagagactct cggcatgctg ttgcccaatt gtttcattga aaatcttatc gactatatga
     2821 agcgcagatg catcgatgat atggacagta ccaggaaaga ccgttcaccc tgctgtgacg
     2881 cacagtttcc ctttgagccg cacatatttg agtactgctt gccacaaagc atatccaaca
     2941 tgtatgacac tacatttttc cggcccggcg tggcaggacc caagtttgca gaggctcccc
     3001 gactggagac tgaggattac ctaggaatga gtggaaatga gagtgcagag tacagcacca
     3061 acggatcatt tacaccgcta ctagtcaaag ccctggtcat cgagttcgag tccaacgtgg
     3121 cctacagcac catctacgca aatattaggc agttctacga gtctgtagag cactggtttc
     3181 agatgcagtt aaaaacggca ccgccggaac ttcaaggagg atggttcacg agcgacctga
     3241 aattttacaa tgtgcaggac accctttcgc acgacacctt tgttgccatt tgtctggcca
     3301 tggctgcatc acttgcagtg ctcctatgct tcaccgtaaa catattgatt tccatctacg
     3361 ccgtactaac cgtgtcgcta agtattttta acaccgtggc tgtgctcatc ctgctcggct
     3421 ggcagcttaa catcttggag agcattgcgg tgagcacggc tatcggtctg gctgtggact
     3481 ttagcctaca ttacggtatt cactaccgga tgtccccggt taaggagaga ttggcagcca
     3541 cacagtttgt actatcccgc atcattggac ccacagtgat ggcggccacc acaactggtc
     3601 tagctggcgg aatcatgatg gcatccaaca tattgcccta catacagatt ggcgtcttcc
     3661 tggtcgttgt catgatcgtt agctggttct atgccacttt ctttctaatg agtctgcttc
     3721 gggttgccgg tcctcagcat ggttttctgg aactcaagtg gccgttgtgg agcaagcgaa
     3781 gcagtggcag cagcaagttc tacgagcgga aacccagcca agtgatcgcc agcgagcagc
     3841 tgctgacccc cacaagctcg gccatcgttg agttggcgaa ctcggagacg cacgaactgg
     3901 agtccctaaa ctccaacagc ctgatcaaaa ccatttcggg catcgaaagc gcccacgcat
     3961 tgtcctcgct gccgagggac ttcgagcact cattccagac gatgcacgag tgcaaatatc
     4021 aaacgtatcc gtctacatcc aatggtacct acccatacga cgtcccagac tacgcttgat
     4081 ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     4141 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     4201 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     4261 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     4321 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     4381 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     4441 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     4501 gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata atcagccata
     4561 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     4621 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     4681 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4741 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccactag tgtcgacgat
     4801 gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg
     4861 cgcgtactcc acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt
     4921 gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt
     4981 ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt
     5041 cagcgggttc tcgacggtca cggcgggcat gtcgacacta gttctagcca gcttttgttc
     5101 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     5161 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     5221 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     5281 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     5341 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5401 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5461 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5521 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5581 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5641 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5701 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     5761 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5821 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5881 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5941 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6001 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6061 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6121 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6181 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6241 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6301 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6361 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6421 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6481 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     6541 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     6601 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     6661 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     6721 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     6781 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     6841 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     6901 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     6961 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7021 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7081 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7141 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7201 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7261 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     7321 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     7381 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     7441 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     7501 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     7561 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     7621 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     7681 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     7741 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     7801 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     7861 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
     7921 tcactatagg gcgaattggg tacgtaccgg gcccctagta tgtatgtaag ttaataaaac
     7981 ccatttttgc ggaaagtaga taaaaaaaac attttttttt tttactgcac tggatatcat
     8041 tgaacttatc tgatcagttt taaatttact tcgatccaag ggtatttgat gtaccaggtt
     8101 ctttcgatta cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct
     8161 tctctgtcca cagaaatatc gccgtctctt tcgccgctgc gtccgctatc tctttcgcca
     8221 ccgtttgtag cgttacgtag cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc
     8281 gtgacgaagc tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc
     8341 ctctcgcttc ttatttttgt ttgttttttg agtgattggg gtggtgattg gttttgggtg
     8401 ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca ggagctatta
     8461 attcgcggag gcagcaaaca cccatctgcc gagcatctga acaatgtgag tagtacatgt
     8521 gcatacatct taagttcact tgatctatag gaactgcgat tgcaacatca aattgtctgc
     8581 ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat tgcacaaaca aatagtgaca
     8641 cgaaacagat tattctggta gctgttctcg ctatataaga caatttttga gatcatatca
     8701 tgatcaagac atctaaaggc attcattttc gactatattc ttttttacaa aaaatataac
     8761 aaccagatat tttaagctga tcctagatgc acaaaaaata aataaaagta taaacctact
     8821 tcgtaggata cttcggggta ctttttgttc ggggttagat gagcataacg cttgtagttg
     8881 atatttgaga tcccctatca ttgcagggtg acagcggagc ggcttcgcag agctgcatta
     8941 accagggctt cgggcaggcc aaaaactacg gcacgctccg gccacccagt ccgccggagg
     9001 actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg cacaatatgg
     9061 acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc aaccggacac
     9121 gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg ctcaagaacg
     9181 gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg tccaattacc
     9241 aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg tgatgggcag
     9301 ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat
     9361 ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg ccaaggagat
     9421 gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc taacggccag
     9481 ggaacacctg attttccaag ccatggtgcg gatgccacga catctgacct atcggcagcg
     9541 agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc agcacacgat
     9601 catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt
     9661 cgcctccgag gctctaaccg atccgccgct tctgatctgc gatgagccca cctccggact
     9721 ggactccttt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa
     9781 gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct ttgacaagat
     9841 ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag ccgtcgactt
     9901 cttttcctag tgagttcgat gtgtttatta agggtatcta gtattacata acatctcaac
     9961 tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac ttttacgtac
    10021 aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc gccaagatat
    10081 gcgacaattt tgccattagc aaagtagccc gggatatgga gcagttgttg gccaccaaaa
    10141 atctggagaa gccactggag cagccggaga atgggtacac ctacaaggcc acctggttca
    10201 tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg
    10261 taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa tgatataacg
    10321 cttacaattc ttggaaacaa attcgctaga ttttagatag aattgcctga ttccacaccc
    10381 ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaagata aataaatttc
    10441 atttaactcg cgaatattaa tgagatgcga gtaacatttt aatttgcaga tggttgccat
    10501 cttgattggc ctcatctttt tgggccaaca actcacgcaa gtgggtgtga tgaatatcaa
    10561 cggagccatc ttcctcttcc tgaccaacat gacctttcaa aacgtctttg ccacgataaa
    10621 tgtaagtcat gtttagaata catttgcatt tcaataattt actaactttc taatgaatcg
    10681 attcgattta ggtgttcacc tcagagctgc cagtttttat gagggaggcc cgaagtcgac
    10741 tttatcgctg tgacacatac tttctgggca aaacgattgc cgaattgccg ctttttctca
    10801 cagtgccact ggtcttcacg gcgattgcct atccgatgat cggactgcgg gccggagtgc
    10861 tgcacttctt caactgcctg gcgctggtca ctctggtggc caatgtgtca acgtccttcg
    10921 gatatctaat atcctgcgcc agctcctcga cctcgatggc gctgtctgtg ggtccgccgg
    10981 ttatcatacc attcctgctc tttggcggct tcttcttgaa ctcgggctcg gtgccagtat
    11041 acctcaaatg gttgtcgtac ctctcatggt tccgttacgc caacgagggt ctgctgatta
    11101 accaatgggc ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc
    11161 ccagttcggg caaggtcatc ctggagacgc ttaacttctc cgccgccgat ctgccgctgg
    11221 actacgtggg tctggccatt ctcatcgtga gcttccgggt gctcgcatat ctggctctaa
    11281 gacttcgggc ccgacgcaag gagtagccga catatatccg aaataactgc ttgttttttt
    11341 tttttaccat tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat
    11401 tatatttgtg ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa
    11461 catccccaca agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac
    11521 cttacatcaa aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat
    11581 acttttcaaa tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg
    11641 ctctttcgct gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg
    11701 gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt
    11761 tcgtctcaat agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat
    11821 ttgtatatat ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt
    11881 aattttttgt gacctgttcg gagtgattag cgttacaatt tgaactgaaa gtgacatcca
    11941 gtgtttgttc cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag tgttgtttat
    12001 atatatcaaa aataacaact ataataataa gaatacattt aatttagaaa atgcttggat
    12061 ttcactggaa ctaggctagc
//
