LOCUS       sequence-246904-        3709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module 0 vector of PlaMiNGo toolkit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB
  TITLE     Targeting specificity of nuclear-encoded organelle proteins with a 
            self-assembling split-fluorescent protein toolkit.
  JOURNAL   J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 
            10.1242/jcs.230839.
  PUBMED    31085714
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-06-3"; volume: "132(11). pii: jcs.230839. doi"; 
            pages: " 10.1242/jcs.230839"
COMMENT     Sequence Label: pTEI122
FEATURES             Location/Qualifiers
     source          1..3709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     103..122
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     356..373
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(490..795)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(592..611)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1115..1798)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     1713..1732
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1799..1901)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1949..1967)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2035..2139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2361..3152
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3323..3709,1..202)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
       61 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      121 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      181 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      241 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      301 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      361 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      421 gccgattcat taatcactct gtggtctcaa atgtgagacc gactggctgt gtataaggga
      481 gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg
      541 gtggctgaga tcagccactt cttccccgat aacggaaacc ggcacactgg ccatatcggt
      601 ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggagac
      661 tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc caggcgtgtc
      721 aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta
      781 aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa
      841 taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt tcggcacgca
      901 gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg acatcatata
      961 tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct gcttcatagc
     1021 atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat accgcaaaaa
     1081 tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc acgcggcgtt
     1141 tacgcccccc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct
     1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     1561 cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa
     1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     1861 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aaggtctcat tcgtgagacc
     1921 acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat
     1981 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2041 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2101 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc
     2161 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     2221 gactgttttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2281 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2341 ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat
     2401 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2461 acggctccgc agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg
     2521 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2581 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2641 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2701 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2761 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2821 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2881 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2941 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3001 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     3061 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cattacgtaa
     3121 aaggcgagat caccaaggta gtcggcaaat aactgtcaga ccaagtttac tcatatatac
     3181 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3241 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcg
//
