LOCUS       sequence-246912-       11145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP
            linker and 24 repeats of PP7 hairpins in 3' UTR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11145)
  AUTHORS   Boersma S, Khuperkar D, Verhagen BMP, Sonneveld S, Grimm JB, Lavis 
            LD, Tanenbaum ME
  TITLE     Multi-Color Single-Molecule Imaging Uncovers Extensive Heterogeneity
            in mRNA Decoding.
  JOURNAL   Cell. 2019 Jun 5. pii: S0092-8674(19)30499-4. doi: 
            10.1016/j.cell.2019.05.001.
  PUBMED    31178119
REFERENCE   2  (bases 1 to 11145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jun 5. pii: S0092-8674(19)30499-4. doi: 10.1016/j.cell.2019.05.001."
COMMENT     Sequence Label: SStart-36xMashTag-BFP-3xStop-24xPP7
            (Sunstart-...
FEATURES             Location/Qualifiers
     source          1..11145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      984..993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1029..1085
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1087..1131
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1134..1190
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1192..1236
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1239..1295
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1297..1341
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1344..1400
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1402..1446
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1449..1505
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1507..1551
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1554..1610
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1612..1656
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1659..1715
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1764..1820
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1822..1866
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1869..1925
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1927..1971
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1974..2030
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2032..2076
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2079..2135
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2137..2181
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2184..2240
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2242..2286
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2289..2345
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2347..2391
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2394..2450
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2452..2496
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2499..2555
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2557..2601
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2604..2660
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2662..2706
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2709..2765
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2767..2811
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2814..2870
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2872..2916
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2919..2975
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2977..3021
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3024..3080
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3082..3126
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3129..3185
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3187..3231
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3234..3290
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3292..3336
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3339..3395
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3397..3441
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3444..3500
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3502..3546
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3585..3641
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3643..3687
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3690..3746
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3748..3792
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3795..3851
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3853..3897
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3900..3956
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3958..4002
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4005..4061
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4063..4107
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4110..4166
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4168..4212
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4215..4271
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4273..4317
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4320..4376
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4378..4422
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4425..4481
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4483..4527
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4588..4632
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4693..4737
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             4740..4796
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4798..4842
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     complement(7156..7173)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7162..7386
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7432..7860
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7519..7538)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7729..7750
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7869..7889)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7874..8203
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8054..8189
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8116..8135
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8251..8298
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8317..8691
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8821..8942
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8858..8877)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8912..8931
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8991..9007)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8991..9007)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9004..9026)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9015..9031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9039..9045,9046..9063,9064..9069))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9084..9105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9222..9239)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9393..9978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9473..9492)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10149..10940,10941..11009))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10772..10791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11010..11114)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccggccgca
     1021 ccggtcaaga agaacttctc agcaagaatt atcatttgga gaacgaagtg gctcgtctca
     1081 agaaacaaga acgagcaaga attactggaa ttggacaagt gggcaagcct ccagaagaac
     1141 tgctttcaaa gaattaccac ctggaaaacg aggttgccag actcaaaaag caagaacgag
     1201 caggaactcc tcgaactcga caagtgggct tctctccaga ggagttactc agcaaaaatt
     1261 atcatttgga gaacgaagtt gcacgactca agaaagaaaa acgaacagga gttactcgag
     1321 ctcgacaagt gggccagcct ccagaggagt tactctcgaa aaattatcat ctcgaaaacg
     1381 aagtggctcg gctcaaaaag caagaacgaa caagaactcc tcgaactgga caagtgggca
     1441 tctctcgaga agagctatta tccaaaaact accacctcga aaacgaggtg gcacgcctca
     1501 aaaagcaaga acgagcaaga attgttggaa ctcgacaagt gggcaagcct tcagaagagc
     1561 tgctatccaa gaattatcat ctcgagaacg aggtggcgcg tctcaagaaa caagaacgag
     1621 caagaattac tcgaactcga caagtgggct tctctcgaga ggaactgctc tcgaagaact
     1681 atcatctgga aaacgaggtc gctcgactca aaaagcaaaa acgaacagga gctgctggag
     1741 ttggacaagt gggccagcct ccagaggaac tactttcaaa gaattaccac ctcgaaaacg
     1801 aagttgctcg actcaaaaag caagaacgaa caggaattac tcgaactcga caagtgggca
     1861 tctctccaga agaattactc agcaaaaatt atcatctgga aaacgaggtg gcgagactca
     1921 aaaagcaaga acgagcagga actcctcgaa ttggacaagt gggcaagcct gcagaagagt
     1981 tgctatcgaa aaattatcat ctggagaacg aagttgcaag gctcaaaaag gaaaaacgag
     2041 caagaactac tcgaactcga caagtgggct tctctgcaga ggagttgctc tcgaaaaact
     2101 accacttgga aaacgaggtc gcgaggctca aaaagcaaaa acgaacaaga gctgctcgag
     2161 ctggacaagt gggccagcct ggagaggagt tactcagcaa aaactatcat ctcgagaacg
     2221 aagtcgcgcg cctcaagaaa gaagaacgaa caagaactac tcgaactgga caagtgggca
     2281 tctctgcaga agaactctta tcgaagaact accacctcga aaacgaggtc gccaggctca
     2341 aaaaggaaga acgagcagga actgctggag ctggacaagt gggcctctct tcagaggaac
     2401 tcctcagcaa gaactaccac ttggagaacg aggtcgcgag actcaagaaa gaaaaacgaa
     2461 caagagctac tcgagctcga caagtgggcc tcccttcaga ggaattactc agcaagaatt
     2521 atcatttgga gaacgaagtc gccaggctca agaaacaaaa acgaacagga attactcgag
     2581 ctggacaagt gggcaagcct ggagaggaat tactcagcaa aaactaccac ttggaaaacg
     2641 aagtcgccag gctcaaaaaa gaaaaacgaa caggagttac tggagctcga caagtgggct
     2701 tctctgcaga agagttactc agcaaaaatt accacttgga gaacgaagtg gcaaggctca
     2761 agaaagaaga acgagcagga actgctggag ctcgacaagt gggcctcttt gcggaggagc
     2821 tcttatcgaa gaactaccac ctcgagaacg aagtcgcccg cctcaagaaa caaaaacgaa
     2881 caggagctac tcgagttgga caagtgggcc tccctggaga agagctcctc agcaagaact
     2941 accacttgga aaacgaggtg gcacgcctca agaaacaaaa acgaacaaga actcctcgag
     3001 ttggacaagt gggcaagcct cgagaagagt tactatccaa gaattatcat ctcgagaacg
     3061 aggtggctcg actcaaaaag caaaaacgaa caggagctcc tggagctcga caagtgggct
     3121 tctctccaga ggaactcctg tcgaagaact atcatctcga aaacgaggtt gcaagactca
     3181 aaaaggaaga acgagcagga actcctggag ctcgacaagt gggcctctct ggagaggaac
     3241 tactctcaaa gaattaccac ctggaaaacg aagttgcacg tctcaagaaa caaaaacgaa
     3301 caggagttac tggagctcga caagtgggcc tccctcgaga agaactgctc tcaaaaaatt
     3361 atcatttgga aaacgaggtt gcacgtctca aaaaggaaaa acgaacaaga attactcgag
     3421 ctcgacaagt gggcaagcct tcagaggaac ttctgtcgaa aaattatcat ctcgagaacg
     3481 aagttgcccg actcaaaaag aaaaaacgaa caagagttac tggagctgga caagtgggct
     3541 tctctggggt tcgaagcagg ggcacgcggt ccggggttcg aagggaggaa cttctcagca
     3601 agaattatca tttggagaac gaagtggctc gtctcaagaa acaaaaacga acaggaactc
     3661 ctggaattgg acaagtgggc aagccttcag aagaactgct ttcaaagaat taccatctgg
     3721 aaaacgaggt tgccagactc aagaagcaag aacgagcagg aattactcga attggacaag
     3781 tgggcctccc tccagaggag ttgctcagca agaattatca tttggagaac gaagttgcac
     3841 gactcaagaa agaagaacga acaggagtta ctcgagctcg acaagtgggc cagcctccag
     3901 aggaattact ctcgaaaaat tatcatctcg aaaacgaagt ggcccggctc aaaaagcaag
     3961 aacgaacagg aactcctcga gctcgacaag tgggcctccc tcgagaggaa ctattatcca
     4021 agaactacca cctcgaaaac gaggtggcac gcctcaagaa gcaaaaacga acaggaattg
     4081 ttggaactcg acaagtgggc aagccttcag aggaactact atccaaaaat tatcatctcg
     4141 agaacgaggt ggcgcgtctc aaaaagcaaa aacgagcagg aactactgga actcgacaag
     4201 tgggcctccc tcgagaggaa ttgctctcga agaactatca tctggagaac gaggtcgctc
     4261 gactcaagaa gcaagaacga acaggagctg ctggaactgg acaagtgggc cagcctccag
     4321 aagaacttct ttcaaaaaat taccacctcg aaaacgaagt tgcgcgactc aaaaagcaaa
     4381 aacgagcagg agctcctcga actcgacaag tgggcatctc tccagaggag ttactcagca
     4441 agaattatca tctggaaaac gaggtggcga gactcaaaaa gcaagaacga gcaagagctc
     4501 ctcgagctgg acaagtgggc atccctgcag aagagttact caggaaaaat tatcatctgg
     4561 aaaacgaagt tgcaaggctc aaaaaggaag aacgaacagg agctactcga actcgacaag
     4621 tgggcatccc tgcagaggag ctgctcagga aaaactacca cttggaaaac gaggtcgcga
     4681 ggctcaagaa gcaagaacga gcaggaactg ctcgagctgg acaagtgggc cagcctggag
     4741 aagagcttct cagcaagaac tatcatctcg agaacgaagt cgcgcgcctc aagaaggaaa
     4801 aacgagcaag aactactcga gctcgacaag tgggcaagcc tgcagggcgt acggggaccg
     4861 gtggagcgag ctcattcggg agaacatgca catcaagcta tacatggagg gcaccgtgga
     4921 caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     4981 catcagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     5041 gagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt
     5101 caagcagtcc ttccctcagg gcttcacatg ggagagagtc accacatacg aagacggggg
     5161 cgtgctcacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
     5221 gatcagaggg gtcaacttca catccaacgg ccctgtcatg cagaagaaaa cactcggctg
     5281 ggaggccttc accgagacgc tgtaccccgc cgacggcggc ctggaaggca gaaacgacat
     5341 ggccctcaag ctcgtgggcg ggagccatct catcgcaaac atcaagacca catacagatc
     5401 caagaaaccc gcaaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga
     5461 aagaatcaag gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag
     5521 atactgcgac ctcccaagca aactggggca caaactcaat ccgcgggggt gagtgagtga
     5581 ggggctagcc cggttgtaca ggtaccggat cctaaggtac ctaattgcct agaaaggagc
     5641 agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc
     5701 gctggctgca gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag
     5761 cagacgatat ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct
     5821 cgctggctgc agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga
     5881 gcagacgata tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc
     5941 tcgctggctg cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg
     6001 agcagacgat atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg
     6061 ctcgctggct gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaag
     6121 gagcagacga tatggcgtcg ctccctgcag gtcgactcta gaaaccagca gagcatatgg
     6181 gctcgctggc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
     6241 ggagcagacg atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg
     6301 ggctcgctgg ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa
     6361 aggagcagac gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat
     6421 gggctcgctg gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga
     6481 aaggagcaga cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata
     6541 tgggctcgct ggctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag
     6601 aaaggagcag acgatatggc gtcgctccct gcaggtcgac tctagaaacc agcagagcat
     6661 atgggctcgc tggctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta
     6721 gaaaggagca gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac cagcagagca
     6781 tatgggctcg ctggctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct
     6841 agaaaggagc agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc
     6901 atatgggctc gctggctgca gtattcccgg gttcattaga tcctaaggta cctaattgcc
     6961 tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc gactctagaa accagcagag
     7021 catatgggct cgctggctgc agtattcccg ggttcattag atctcgcgaa gggcgaattc
     7081 acgaggcgcg cccggatcgc gatcgcaatc ttaattaact cgagtctaga gggcccgttt
     7141 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     7201 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     7261 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     7321 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     7381 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     7441 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7501 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7561 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7621 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7681 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7741 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7801 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7861 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7921 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7981 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     8041 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8101 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     8161 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     8221 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     8281 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     8341 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     8401 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     8461 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     8521 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     8581 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     8641 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     8701 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     8761 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     8821 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8881 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8941 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     9001 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9061 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9121 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9181 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9241 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9301 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9361 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9421 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9481 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9541 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9601 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9661 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9721 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9781 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9841 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9901 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc
     9961 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10021 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10081 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10141 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10201 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10261 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10321 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10381 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10441 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10501 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10561 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10621 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10681 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10741 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10801 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10861 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10921 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10981 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11041 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11101 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
