LOCUS       sequence-246915-       15111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15111)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX063
FEATURES             Location/Qualifiers
     source          1..15111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5257..5281)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5310..5399
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5433..5685
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6114..6366)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6834..7859)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6834..7859)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7869..8213)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8323..8340)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8323..8339)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8332..8354)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8479..8498)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8689..8710
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8957..8981
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10577..11725)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12024..12818)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13010..13777
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14470..15101
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg cagagtcagt tggtgtgcag
     4621 tgggtgtagg acaattcttc tttatccgag aggagctact aatgtctgct gtgcagtgtg
     4681 caatgccctc acccctgtcc cacctcctgg tatctgtctt ttctgcctct actatcagtg
     4741 ctgtgtgata agaacatgca tcactagttt atttggcact tctgatatga tttctgtcta
     4801 gccaatggtg aatgaaaggg aaaagaggaa gaaatagaat ggaaatggct cagttgatat
     4861 gtggaggctg tcgcacctta ctgatgcatc cacgtggggc aactagtgtg agatgcgcct
     4921 gctgtcatac ggtgaacctt gtgccaggtc ctaaccagtt tgctcatgtc aactgtggaa
     4981 attgtcgtac tatgctgatg tatccatgcg gagctccatc agttaaatgt gccatatgcc
     5041 actatatcac acacgttaat gccggagaag gaagagtcca tgttcctgtg cacaccccaa
     5101 atggcggtgc tacatcctct gcaggaccca cttcctcaac agctgcagcc cgttcacaga
     5161 atcaaaccgt ggtcgttcag aaccctatgt ctttagatga aagtggcaag ttggtgagca
     5221 atgttgttgt tggtgtgacg acaacaactc ccggggaccc agctttcttg tacaaagtgg
     5281 ttgataacag cgggttaatt aacatctttt acccatacga tgttcctgac tatgcgggct
     5341 atccctatga cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg
     5401 ctcagtgcag cgcttagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     5461 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     5521 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     5581 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     5641 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttagcttcat
     5701 caacgcaaga catgcgcacg accgtctgac aggagaggaa tttccgacga gcacagaaag
     5761 gacttgctct tggacgtagg cctatttctc aggcacatgt atcaagtgtt cggacgtggg
     5821 ttttcgatgg tgtatcagcc gccgccaact gggagatgag gaggctttct tggggggcag
     5881 tcagcagttc atttcacaag acagaggaac ttgtaaggag atgcactgat ttatcttggc
     5941 gcaaaccagc aggacgaatt agtgggaata gcccgcgaat atctaagtta tgcctgtcgg
     6001 catgagcaga aacttccaat tcgaaacagt ttggagaggt tgtttttggg catacctttt
     6061 gttagtcagc ctctcgattg ctcatcgtca ttacacagta ccgaagtttg atcgatctag
     6121 taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc
     6181 tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa
     6241 cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
     6301 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
     6361 acgatctgct tcgacgcact ccttctttac tccaccatct cgtccttatt gaaaacgtgg
     6421 gtagcaccaa aacgaatcaa gtcgctggaa ctgaagttac caatcacgct ggatgatttg
     6481 ccagttggat taatcttgcc tttccccgca tgaataatat tgatgaatgc atgcgtgagg
     6541 ggtatttcga ttttggcaat agctgcaatt gccgcgacat cctccaacga gcataattct
     6601 tcagaaaaat agcgatgttc catgttgtca gggcatgcat gatgcacgtt atgaggtgac
     6661 ggtgctaggc agtattccct caaagtttca tagtcagtat catattcatc attgcattcc
     6721 tgcaagagag aattgagacg caatccacac gctgcggcaa ccttccggcg ttcgtggtct
     6781 atttgctctt ggacgttgca aacgtaagtg ttggatcccg gtcggcatct actctattcc
     6841 tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag
     6901 ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct
     6961 ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg
     7021 tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc
     7081 ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa
     7141 gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc
     7201 gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag
     7261 ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc
     7321 tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga
     7381 tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt
     7441 ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca
     7501 tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc
     7561 aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca
     7621 atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga
     7681 tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca
     7741 tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg
     7801 tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata
     7861 tcggggtcgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
     7921 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
     7981 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     8041 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
     8101 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
     8161 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
     8221 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
     8281 ccatgttgac ggatctctag gacgcgtcct agaagctaat tcactggccg tcgttttaca
     8341 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     8401 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     8461 cagcctgaat ggcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     8521 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     8581 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     8641 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     8701 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     8761 tgccgatttc ggaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
     8821 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
     8881 actggtgaaa agaaaaacca ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
     8941 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
     9001 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
     9061 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
     9121 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
     9181 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa atatcatctc cctcgcagag
     9241 atccgaatta tcagccttct tattcatttc tcgcttaacc gtgacaggct gtcgatcttg
     9301 agaactatgc cgacataata ggaaatcgct ggataaagcc gctgaggaag ctgagtggcg
     9361 ctatttcttt agaagtgaac gttgacgata tcaactcccc tatccattgc tcaccgaatg
     9421 gtacaggtcg gggacccgaa gttccgactg tcggcctgat gcatccccgg ctgatcgacc
     9481 ccagatctgg ggctgagaaa gcccagtaag gaaacaactg taggttcgag tcgcgagatc
     9541 ccccggaacc aaaggaagta ggttaaaccc gctccgatca ggccgagcca cgccaggccg
     9601 agaacattgg ttcctgtagg catcgggatt ggcggatcaa acactaaagc tactggaacg
     9661 agcagaagtc ctccggccgc cagttgccag gcggtaaagg tgagcagagg cacgggaggt
     9721 tgccacttgc gggtcagcac ggttccgaac gccatggaaa ccgcccccgc caggcccgct
     9781 gcgacgccga caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc cacgcccgca
     9841 gttccgcaaa tagcccccag gaccgccatc aatcgtatcg ggctacctag cagagcggca
     9901 gagatgaaca cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac cccgcccggc
     9961 aggcggtaga ccgaaataaa caacaagctc cagaatagcg aaatattaag tgcgccgagg
    10021 atgaagatgc gcatccacca gattcccgtt ggaatctgtc ggacgatcat cacgagcaat
    10081 aaacccgccg gcaacgcccg cagcagcata ccggcgaccc ctcggcctcg ctgttcgggc
    10141 tccacgaaaa cgccggacag atgcgccttg tgagcgtcct tggggccgtc ctcctgtttg
    10201 aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc cgaatgccac ggcatctcgc
    10261 aaccgttcag cgaacgcctc catgggcttt ttctcctcgt gctcgtaaac ggacccgaac
    10321 atctctggag ctttcttcag ggccgacaat cggatctcgc ggaaatcctg cacgtcggcc
    10381 gctccaagcc gtcgaatctg agccttaatc acaattgtca attttaatcc tctgtttatc
    10441 ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag
    10501 cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc ccagccggaa
    10561 ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt
    10621 ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg cgccacttct
    10681 tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg agcgggtacg
    10741 gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt
    10801 acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt
    10861 cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg aagcggtgca
    10921 gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct
    10981 gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc gaccagccca
    11041 ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg
    11101 cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg
    11161 tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc
    11221 gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg
    11281 ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt tccttgatct
    11341 gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt tttgccaggt
    11401 cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg
    11461 acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg gcggccgatg
    11521 gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt
    11581 gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg acggtgcggc
    11641 ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct tgcctgtatg
    11701 ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg actcacgccg
    11761 gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc agataatcca
    11821 ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg tacttggtat
    11881 tccgaatctt gccctgcacg aataccagcg accccttgcc caaatacttg ccgtgggcct
    11941 cggcctgaga gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccgg
    12001 catcgttgcg ccacatctag gtactaaaac aattcatcca gtaaaatata atattttatt
    12061 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
    12121 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
    12181 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
    12241 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
    12301 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
    12361 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
    12421 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
    12481 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
    12541 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
    12601 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
    12661 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc
    12721 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
    12781 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc
    12841 tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt
    12901 tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt
    12961 tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatgg gtgatgctgc
    13021 caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta
    13081 tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg
    13141 gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca
    13201 ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat
    13261 gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa
    13321 actcaggccg cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc
    13381 ggaaatggac gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta
    13441 cgcgtatgac agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc
    13501 gacgctgggg cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga
    13561 tggctggccg ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata
    13621 tacgcagcga attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg
    13681 gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct
    13741 gaacataaaa cactatcaat aagttggagt cattacccaa ttatgataga atttacaagc
    13801 tataaggtta ttgtcctggg tttcaagcat tagtccatgc aagtttttat gctttgccca
    13861 ttctatagat atattgataa gcgcgctgcc tatgccttgc cccctgaaat ccttacatac
    13921 ggcgatatct tctatataaa agatatatta tcttatcagt attgtcaata tattcaaggc
    13981 aatctgcctc ctcatcctct tcatcctctt cgtcttggta gctttttaaa tatggcgctt
    14041 catagagtaa ttctgtaaag gtccaattct cgttttcata cctcggtata atcttaccta
    14101 tcacctcaaa tggttcgctg ggtttatcgc acccccgaac acgagcacgg cacccgcgac
    14161 cactatgcca agaatgccca aggtaaaaat tgccggcccc gccatgaagt ccgtgaatgc
    14221 cccgacggcc gaagtgaagg gcaggccgcc acccaggccg ccgccctcac tgcccggcac
    14281 ctggtcgctg aatgtcgatg ccagcacctg cggcacgtca atgcttccgg gcgtcgcgct
    14341 cgggctgatc gcccatcccg ttactgcccc gatcccggca atggcaagga ctgccagcgc
    14401 tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg gggatgggag
    14461 gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg
    14521 gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag
    14581 caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg
    14641 attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac
    14701 tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt
    14761 gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt
    14821 aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc
    14881 gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt
    14941 ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc
    15001 cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg
    15061 ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt cggaaaggcg c
//
