LOCUS       sequence-246917-       14909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14909)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 14909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX064
FEATURES             Location/Qualifiers
     source          1..14909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5055..5079)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5108..5197
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5231..5483
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(5912..6164)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6632..7657)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6632..7657)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7667..8011)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8121..8138)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8121..8137)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8130..8152)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8277..8296)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8487..8508
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8755..8779
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10375..11523)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11822..12616)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12808..13575
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14268..14899
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgccagt
     4621 tcctcttgcc ccatatccta caccaacaac gccatttaca ccgcctgcta acggtacaca
     4681 gagccagctc gtgtgctctg gatgtagaaa tcttttactc tatccaattg gagcaacatc
     4741 tgtctgctgt gcagtttgca atacagtcac agcagtacca cctcctggca ctgagatggc
     4801 tcagttggtt tgcggaggct gccacacatt actgatgtac attcgtggag caacaagtgt
     4861 gcagtgttct tgttgtcaca cagtcaattt agctatggaa gcaaatcagg tagcacatgt
     4921 gaattgtggc aactgccgca tgctgttgat gtaccaatat ggggcacgat cagtgaaatg
     4981 tgcagtgtgc agttttgtga cagcggtagg ggtttcaacg agcacaactg agcaaaagct
     5041 taacagcccc ggggacccag ctttcttgta caaagtggtt gataacagcg ggttaattaa
     5101 catcttttac ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta
     5161 tgcaggatcc tatccatatg acgttccaga ttacgctgct cagtgcagcg cttagagctc
     5221 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     5281 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     5341 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     5401 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     5461 ggtgtcatct atgttactag atcgggaatt agcttcatca acgcaagaca tgcgcacgac
     5521 cgtctgacag gagaggaatt tccgacgagc acagaaagga cttgctcttg gacgtaggcc
     5581 tatttctcag gcacatgtat caagtgttcg gacgtgggtt ttcgatggtg tatcagccgc
     5641 cgccaactgg gagatgagga ggctttcttg gggggcagtc agcagttcat ttcacaagac
     5701 agaggaactt gtaaggagat gcactgattt atcttggcgc aaaccagcag gacgaattag
     5761 tgggaatagc ccgcgaatat ctaagttatg cctgtcggca tgagcagaaa cttccaattc
     5821 gaaacagttt ggagaggttg tttttgggca taccttttgt tagtcagcct ctcgattgct
     5881 catcgtcatt acacagtacc gaagtttgat cgatctagta acatagatga caccgcgcgc
     5941 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat
     6001 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt
     6061 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca
     6121 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatctgcttc gacgcactcc
     6181 ttctttactc caccatctcg tccttattga aaacgtgggt agcaccaaaa cgaatcaagt
     6241 cgctggaact gaagttacca atcacgctgg atgatttgcc agttggatta atcttgcctt
     6301 tccccgcatg aataatattg atgaatgcat gcgtgagggg tatttcgatt ttggcaatag
     6361 ctgcaattgc cgcgacatcc tccaacgagc ataattcttc agaaaaatag cgatgttcca
     6421 tgttgtcagg gcatgcatga tgcacgttat gaggtgacgg tgctaggcag tattccctca
     6481 aagtttcata gtcagtatca tattcatcat tgcattcctg caagagagaa ttgagacgca
     6541 atccacacgc tgcggcaacc ttccggcgtt cgtggtctat ttgctcttgg acgttgcaaa
     6601 cgtaagtgtt ggatcccggt cggcatctac tctattcctt tgccctcgga cgagtgctgg
     6661 ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc
     6721 ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc
     6781 atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt
     6841 ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat
     6901 gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga
     6961 agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg
     7021 ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt
     7081 gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg
     7141 acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg
     7201 cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga
     7261 acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct
     7321 gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc
     7381 gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg
     7441 ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc
     7501 agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt
     7561 cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact
     7621 tctcgacaga cgtcgcggtg agttcaggct ttttcatatc ggggtcgtcc tctccaaatg
     7681 aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc
     7741 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     7801 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     7861 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     7921 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     7981 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     8041 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttgacgg atctctagga
     8101 cgcgtcctag aagctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     8161 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     8221 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc
     8281 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8341 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8401 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8461 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8521 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc
     8581 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
     8641 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
     8701 ccagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     8761 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     8821 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     8881 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     8941 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     9001 gttgctgcct gtgatcaaat atcatctccc tcgcagagat ccgaattatc agccttctta
     9061 ttcatttctc gcttaaccgt gacaggctgt cgatcttgag aactatgccg acataatagg
     9121 aaatcgctgg ataaagccgc tgaggaagct gagtggcgct atttctttag aagtgaacgt
     9181 tgacgatatc aactccccta tccattgctc accgaatggt acaggtcggg gacccgaagt
     9241 tccgactgtc ggcctgatgc atccccggct gatcgacccc agatctgggg ctgagaaagc
     9301 ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg
     9361 ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca
     9421 tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca
     9481 gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg
     9541 ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg
     9601 ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga
     9661 ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg
     9721 gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca
     9781 acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga
     9841 ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca
     9901 gcagcatacc ggcgacccct cggcctcgct gttcgggctc cacgaaaacg ccggacagat
     9961 gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa gaccgacagc ccaatgatct
    10021 cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg aacgcctcca
    10081 tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct ttcttcaggg
    10141 ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt cgaatctgag
    10201 ccttaatcac aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt
    10261 gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa
    10321 tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg
    10381 tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa
    10441 ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc
    10501 gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata
    10561 gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt
    10621 gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga
    10681 cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg
    10741 cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc
    10801 ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa
    10861 cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca
    10921 caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt
    10981 gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt
    11041 ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg
    11101 cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa
    11161 cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt
    11221 cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc
    11281 ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc
    11341 cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga
    11401 ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc
    11461 ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt
    11521 cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg
    11581 atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt
    11641 cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa
    11701 taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt
    11761 gatgcggaag aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctaggt
    11821 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
    11881 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
    11941 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
    12001 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
    12061 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
    12121 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
    12181 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
    12241 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
    12301 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
    12361 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
    12421 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
    12481 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
    12541 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
    12601 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
    12661 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
    12721 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
    12781 ggagccgatt ttgaaaccac aattatgggt gatgctgcca acttactgat ttagtgtatg
    12841 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
    12901 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc
    12961 tcactgccgt aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca
    13021 ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggcaacag cccgcattat
    13081 gggcgttggc ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc
    13141 tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc
    13201 tatgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag
    13261 acggttgttg cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc
    13321 ctgctgtcac cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc
    13381 cgcctgaagg gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat
    13441 aacctgaatc tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa
    13501 tcggtggagc tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa
    13561 gttggagtca ttacccaatt atgatagaat ttacaagcta taaggttatt gtcctgggtt
    13621 tcaagcatta gtccatgcaa gtttttatgc tttgcccatt ctatagatat attgataagc
    13681 gcgctgccta tgccttgccc cctgaaatcc ttacatacgg cgatatcttc tatataaaag
    13741 atatattatc ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct catcctcttc
    13801 atcctcttcg tcttggtagc tttttaaata tggcgcttca tagagtaatt ctgtaaaggt
    13861 ccaattctcg ttttcatacc tcggtataat cttacctatc acctcaaatg gttcgctggg
    13921 tttatcgcac ccccgaacac gagcacggca cccgcgacca ctatgccaag aatgcccaag
    13981 gtaaaaattg ccggccccgc catgaagtcc gtgaatgccc cgacggccga agtgaagggc
    14041 aggccgccac ccaggccgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc
    14101 agcacctgcg gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt
    14161 actgccccga tcccggcaat ggcaaggact gccagcgctg ccatttttgg ggtgaggccg
    14221 ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag
    14281 ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc
    14341 ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg
    14401 gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc
    14461 ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga
    14521 tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat
    14581 ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat
    14641 ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa
    14701 cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag
    14761 ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt
    14821 cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc
    14881 aaccagcccg gtgagcgtcg gaaaggcgc
//
