LOCUS       sequence-246920-       15029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15029)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX067
FEATURES             Location/Qualifiers
     source          1..15029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5175..5199)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5228..5317
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5351..5603
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6032..6284)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6752..7777)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6752..7777)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7787..8131)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8241..8258)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8241..8257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8250..8272)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8397..8416)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8607..8628
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8875..8899
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10495..11643)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11942..12736)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12928..13695
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14388..15019
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgtcact
     4621 cgacatgaca tctttgattt cttcggaacg tgtttgttac ttgcagtgca acttttgcaa
     4681 caccgttctt gcggttagcg ttccatgcag caacatattt gccattgtga cagtaagatg
     4741 tggccactgc tctaatttgc tttctgttag tatgcagtct tcacctcagc ctctccccct
     4801 tccagatcca cagaagcagc aaagacaaca gtcaaatgtt gaaaatggca gtaaagcctg
     4861 cgagtcatca tcttcttcaa agttcaacag attttctgcc attgattatt ctcctcaaat
     4921 tgaacctcga atcacaccta ttcgcccgcc ggagaaaaga caacgtcttc cttctgctta
     4981 caaccgattc atcaaggaag agatccaaag gataaaggca agcaatcctg atattagtca
     5041 ccggcaagct tttagcactg ctgccaaaaa ttgggcgcat tttcctcata tccactttgg
     5101 actcaagcaa gccaaattgg accatgcagt tgcaggagag agtcctcata aatctcttgg
     5161 tctttacccc ggggacccag ctttcttgta caaagtggtt gataacagcg ggttaattaa
     5221 catcttttac ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta
     5281 tgcaggatcc tatccatatg acgttccaga ttacgctgct cagtgcagcg cttagagctc
     5341 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     5401 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     5461 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     5521 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     5581 ggtgtcatct atgttactag atcgggaatt agcttcatca acgcaagaca tgcgcacgac
     5641 cgtctgacag gagaggaatt tccgacgagc acagaaagga cttgctcttg gacgtaggcc
     5701 tatttctcag gcacatgtat caagtgttcg gacgtgggtt ttcgatggtg tatcagccgc
     5761 cgccaactgg gagatgagga ggctttcttg gggggcagtc agcagttcat ttcacaagac
     5821 agaggaactt gtaaggagat gcactgattt atcttggcgc aaaccagcag gacgaattag
     5881 tgggaatagc ccgcgaatat ctaagttatg cctgtcggca tgagcagaaa cttccaattc
     5941 gaaacagttt ggagaggttg tttttgggca taccttttgt tagtcagcct ctcgattgct
     6001 catcgtcatt acacagtacc gaagtttgat cgatctagta acatagatga caccgcgcgc
     6061 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat
     6121 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt
     6181 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca
     6241 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatctgcttc gacgcactcc
     6301 ttctttactc caccatctcg tccttattga aaacgtgggt agcaccaaaa cgaatcaagt
     6361 cgctggaact gaagttacca atcacgctgg atgatttgcc agttggatta atcttgcctt
     6421 tccccgcatg aataatattg atgaatgcat gcgtgagggg tatttcgatt ttggcaatag
     6481 ctgcaattgc cgcgacatcc tccaacgagc ataattcttc agaaaaatag cgatgttcca
     6541 tgttgtcagg gcatgcatga tgcacgttat gaggtgacgg tgctaggcag tattccctca
     6601 aagtttcata gtcagtatca tattcatcat tgcattcctg caagagagaa ttgagacgca
     6661 atccacacgc tgcggcaacc ttccggcgtt cgtggtctat ttgctcttgg acgttgcaaa
     6721 cgtaagtgtt ggatcccggt cggcatctac tctattcctt tgccctcgga cgagtgctgg
     6781 ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc
     6841 ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc
     6901 atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt
     6961 ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat
     7021 gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga
     7081 agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg
     7141 ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt
     7201 gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg
     7261 acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg
     7321 cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga
     7381 acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct
     7441 gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc
     7501 gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg
     7561 ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc
     7621 agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt
     7681 cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact
     7741 tctcgacaga cgtcgcggtg agttcaggct ttttcatatc ggggtcgtcc tctccaaatg
     7801 aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc
     7861 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     7921 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     7981 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     8041 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     8101 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     8161 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttgacgg atctctagga
     8221 cgcgtcctag aagctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     8281 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     8341 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc
     8401 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8461 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8521 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8581 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8641 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc
     8701 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
     8761 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
     8821 ccagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     8881 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     8941 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     9001 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     9061 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     9121 gttgctgcct gtgatcaaat atcatctccc tcgcagagat ccgaattatc agccttctta
     9181 ttcatttctc gcttaaccgt gacaggctgt cgatcttgag aactatgccg acataatagg
     9241 aaatcgctgg ataaagccgc tgaggaagct gagtggcgct atttctttag aagtgaacgt
     9301 tgacgatatc aactccccta tccattgctc accgaatggt acaggtcggg gacccgaagt
     9361 tccgactgtc ggcctgatgc atccccggct gatcgacccc agatctgggg ctgagaaagc
     9421 ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg
     9481 ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca
     9541 tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca
     9601 gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg
     9661 ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg
     9721 ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga
     9781 ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg
     9841 gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca
     9901 acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga
     9961 ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca
    10021 gcagcatacc ggcgacccct cggcctcgct gttcgggctc cacgaaaacg ccggacagat
    10081 gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa gaccgacagc ccaatgatct
    10141 cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg aacgcctcca
    10201 tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct ttcttcaggg
    10261 ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt cgaatctgag
    10321 ccttaatcac aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt
    10381 gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa
    10441 tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg
    10501 tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa
    10561 ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc
    10621 gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata
    10681 gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt
    10741 gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga
    10801 cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg
    10861 cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc
    10921 ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa
    10981 cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca
    11041 caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt
    11101 gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt
    11161 ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg
    11221 cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa
    11281 cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt
    11341 cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc
    11401 ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc
    11461 cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga
    11521 ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc
    11581 ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt
    11641 cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg
    11701 atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt
    11761 cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa
    11821 taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt
    11881 gatgcggaag aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctaggt
    11941 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
    12001 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
    12061 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
    12121 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
    12181 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
    12241 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
    12301 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
    12361 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
    12421 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
    12481 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
    12541 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
    12601 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
    12661 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
    12721 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
    12781 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
    12841 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
    12901 ggagccgatt ttgaaaccac aattatgggt gatgctgcca acttactgat ttagtgtatg
    12961 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
    13021 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc
    13081 tcactgccgt aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca
    13141 ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggcaacag cccgcattat
    13201 gggcgttggc ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc
    13261 tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc
    13321 tatgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag
    13381 acggttgttg cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc
    13441 ctgctgtcac cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc
    13501 cgcctgaagg gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat
    13561 aacctgaatc tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa
    13621 tcggtggagc tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa
    13681 gttggagtca ttacccaatt atgatagaat ttacaagcta taaggttatt gtcctgggtt
    13741 tcaagcatta gtccatgcaa gtttttatgc tttgcccatt ctatagatat attgataagc
    13801 gcgctgccta tgccttgccc cctgaaatcc ttacatacgg cgatatcttc tatataaaag
    13861 atatattatc ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct catcctcttc
    13921 atcctcttcg tcttggtagc tttttaaata tggcgcttca tagagtaatt ctgtaaaggt
    13981 ccaattctcg ttttcatacc tcggtataat cttacctatc acctcaaatg gttcgctggg
    14041 tttatcgcac ccccgaacac gagcacggca cccgcgacca ctatgccaag aatgcccaag
    14101 gtaaaaattg ccggccccgc catgaagtcc gtgaatgccc cgacggccga agtgaagggc
    14161 aggccgccac ccaggccgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc
    14221 agcacctgcg gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt
    14281 actgccccga tcccggcaat ggcaaggact gccagcgctg ccatttttgg ggtgaggccg
    14341 ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag
    14401 ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc
    14461 ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg
    14521 gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc
    14581 ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga
    14641 tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat
    14701 ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat
    14761 ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa
    14821 cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag
    14881 ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt
    14941 cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc
    15001 aaccagcccg gtgagcgtcg gaaaggcgc
//
