LOCUS       sequence-246923-       15979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15979)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX070
FEATURES             Location/Qualifiers
     source          1..15979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6093..6117)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6146..6235
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6269..6521
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6950..7202)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7670..8695)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7670..8695)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8705..9049)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9191..9208)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9191..9207)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9200..9222)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9347..9366)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9557..9578
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9825..9849
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11445..12593)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12892..13686)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13878..14645
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15338..15969
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgattaa
     4621 gggcatggta ggagatgata gtatgtcaaa tttaacatct gcatctaatg aagcaagcat
     4681 atcttcaagt aatagagctg aaattggtag tactctctac ccacagcatc ttgcaccaac
     4741 aaacattcaa acacaaccag ctgctaataa gaagaagaga aatctaccag gcaatccaga
     4801 tccagaagca gaagtgatag ctttatctcc aaaaagtcta ttggcaacaa atagatttgt
     4861 atgtgagatc tgtaacaaag gatttcaaag agatcaaaat cttcaactac acagaagagg
     4921 tcataatttg ccatggaaac taaagcaaag aacaaacaag gaagtgaaga agaaagtata
     4981 tgtgtgtcca gagccttcat gtgtacacca tcatccttca agggcactag gggatttgac
     5041 tggaattaag aagcattttt gtagaaaaca tggtgagaag aaatggaagt gtgataaatg
     5101 ttcaaagaga tatgcagttc agtctgattg gaaagctcat tccaagattt gtggtactag
     5161 agagtataga tgtgattgtg gaaccctttt ctctaggagg gatagcttca tcacacatag
     5221 agctttttgt gatgcattag cagaagaaag tgtaagaaca acaaatcctc taatttcttc
     5281 ttcttcaact tcccatcacc ttaatctcca taccctccaa aacttccaac tcaaacaaga
     5341 acaacaacaa caacttaata atttcaacct tcttcctccc cacccttcga acgagctttc
     5401 gtctcagtta ttcattcata accctactag tagtagcagt aacaatatat ttggagcagc
     5461 ggcagcagca acatcagctc acatgagtgc gactgcatta ctacaaaaag cagcacaaat
     5521 tggtgtcact tcttctaatt ctaattctgc tcctagtttt ttcagtccta ctactattgc
     5581 tactacaact ggcatggcag gtggtggtga tttagcctca cgtgaatatc atcagtttca
     5641 aaatattagt gccaataata gtactatggc tcccactact ggtaatttta ttactagtca
     5701 tggcttggaa tcttatggca acaacaacaa caataacaga ggtgcttttg atcattttag
     5761 tgtcaactct ggatttatgg aacaaatcca tgacatgcaa aacatgatgg ctgctgctac
     5821 tactactgct ttgtcttgta ctactaatag tgttggtttt gatgaaggat ttggtggagc
     5881 aaataatcct ttgataatga gaggtaaaga aaataatgaa ggattgacaa gagatttctt
     5941 gggacttaaa gcatttccac atcataggga ctttctcaat aatactacta caactggtct
     6001 tcatcatcac atgggttctt cttctgctgc tgctgcttca tttgaccatc agcaacataa
     6061 tcaaaatcaa tcaccctggc aaaatcccgg ggacccagct ttcttgtaca aagtggttga
     6121 taacagcggg ttaattaaca tcttttaccc atacgatgtt cctgactatg cgggctatcc
     6181 ctatgacgtc ccggactatg caggatccta tccatatgac gttccagatt acgctgctca
     6241 gtgcagcgct tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     6301 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     6361 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     6421 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     6481 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattag cttcatcaac
     6541 gcaagacatg cgcacgaccg tctgacagga gaggaatttc cgacgagcac agaaaggact
     6601 tgctcttgga cgtaggccta tttctcaggc acatgtatca agtgttcgga cgtgggtttt
     6661 cgatggtgta tcagccgccg ccaactggga gatgaggagg ctttcttggg gggcagtcag
     6721 cagttcattt cacaagacag aggaacttgt aaggagatgc actgatttat cttggcgcaa
     6781 accagcagga cgaattagtg ggaatagccc gcgaatatct aagttatgcc tgtcggcatg
     6841 agcagaaact tccaattcga aacagtttgg agaggttgtt tttgggcata ccttttgtta
     6901 gtcagcctct cgattgctca tcgtcattac acagtaccga agtttgatcg atctagtaac
     6961 atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc
     7021 gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc
     7081 atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc
     7141 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga
     7201 tctgcttcga cgcactcctt ctttactcca ccatctcgtc cttattgaaa acgtgggtag
     7261 caccaaaacg aatcaagtcg ctggaactga agttaccaat cacgctggat gatttgccag
     7321 ttggattaat cttgcctttc cccgcatgaa taatattgat gaatgcatgc gtgaggggta
     7381 tttcgatttt ggcaatagct gcaattgccg cgacatcctc caacgagcat aattcttcag
     7441 aaaaatagcg atgttccatg ttgtcagggc atgcatgatg cacgttatga ggtgacggtg
     7501 ctaggcagta ttccctcaaa gtttcatagt cagtatcata ttcatcattg cattcctgca
     7561 agagagaatt gagacgcaat ccacacgctg cggcaacctt ccggcgttcg tggtctattt
     7621 gctcttggac gttgcaaacg taagtgttgg atcccggtcg gcatctactc tattcctttg
     7681 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
     7741 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
     7801 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
     7861 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg
     7921 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
     7981 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
     8041 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
     8101 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
     8161 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
     8221 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
     8281 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
     8341 tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
     8401 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt
     8461 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
     8521 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
     8581 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
     8641 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatcgg
     8701 ggtcgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata
     8761 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
     8821 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     8881 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     8941 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
     9001 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
     9061 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
     9121 gttgacggat ctctaggacg cgtcgacgcg tcgacgcgtc gacgcgtcga cgcgtcctag
     9181 aagctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     9241 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     9301 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc
     9361 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9421 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     9481 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9541 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9601 ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc aaacaggatt
     9661 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     9721 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt
     9781 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     9841 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     9901 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     9961 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
    10021 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
    10081 gtgatcaaat atcatctccc tcgcagagat ccgaattatc agccttctta ttcatttctc
    10141 gcttaaccgt gacaggctgt cgatcttgag aactatgccg acataatagg aaatcgctgg
    10201 ataaagccgc tgaggaagct gagtggcgct atttctttag aagtgaacgt tgacgatatc
    10261 aactccccta tccattgctc accgaatggt acaggtcggg gacccgaagt tccgactgtc
    10321 ggcctgatgc atccccggct gatcgacccc agatctgggg ctgagaaagc ccagtaagga
    10381 aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg ttaaacccgc
    10441 tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca tcgggattgg
    10501 cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca gttgccaggc
    10561 ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg ttccgaacgc
    10621 catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg ctgcgtttgg
    10681 tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga ccgccatcaa
    10741 tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg gctgcacagc
    10801 gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca acaagctcca
    10861 gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga ttcccgttgg
    10921 aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca gcagcatacc
    10981 ggcgacccct cggcctcgct gttcgggctc cacgaaaacg ccggacagat gcgccttgtg
    11041 agcgtccttg gggccgtcct cctgtttgaa gaccgacagc ccaatgatct cgccgtcgat
    11101 gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg aacgcctcca tgggcttttt
    11161 ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct ttcttcaggg ccgacaatcg
    11221 gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt cgaatctgag ccttaatcac
    11281 aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt gcgtcccgag
    11341 cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa tgccagtaaa
    11401 gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc
    11461 accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag
    11521 gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg
    11581 cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc
    11641 cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg
    11701 ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg
    11761 ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg
    11821 tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg
    11881 taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg
    11941 atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg
    12001 tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg
    12061 aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg
    12121 gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg
    12181 aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc
    12241 ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc
    12301 atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga
    12361 cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg
    12421 ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt
    12481 tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac
    12541 cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct
    12601 ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg
    12661 cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc
    12721 gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagcgac
    12781 cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag
    12841 aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctaggt actaaaacaa
    12901 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
    12961 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
    13021 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
    13081 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
    13141 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
    13201 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
    13261 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
    13321 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
    13381 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
    13441 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
    13501 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
    13561 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
    13621 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
    13681 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
    13741 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
    13801 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
    13861 ttgaaaccac aattatgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt
    13921 tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg
    13981 gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc tcactgccgt
    14041 aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc
    14101 attgatatgg ccatgaatgg cgttggatgc cgggcaacag cccgcattat gggcgttggc
    14161 ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
    14221 agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg
    14281 ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg
    14341 cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac
    14401 cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg
    14461 gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc
    14521 tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc
    14581 tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca
    14641 ttacccaatt atgatagaat ttacaagcta taaggttatt gtcctgggtt tcaagcatta
    14701 gtccatgcaa gtttttatgc tttgcccatt ctatagatat attgataagc gcgctgccta
    14761 tgccttgccc cctgaaatcc ttacatacgg cgatatcttc tatataaaag atatattatc
    14821 ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct catcctcttc atcctcttcg
    14881 tcttggtagc tttttaaata tggcgcttca tagagtaatt ctgtaaaggt ccaattctcg
    14941 ttttcatacc tcggtataat cttacctatc acctcaaatg gttcgctggg tttatcgcac
    15001 ccccgaacac gagcacggca cccgcgacca ctatgccaag aatgcccaag gtaaaaattg
    15061 ccggccccgc catgaagtcc gtgaatgccc cgacggccga agtgaagggc aggccgccac
    15121 ccaggccgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg
    15181 gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga
    15241 tcccggcaat ggcaaggact gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg
    15301 aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa
    15361 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa
    15421 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa
    15481 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc
    15541 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct
    15601 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt
    15661 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct
    15721 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg
    15781 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt
    15841 tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt cgacggcgtt
    15901 tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg
    15961 gtgagcgtcg gaaaggcgc
//
