LOCUS       sequence-246924-       17077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17077)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 17077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX071
FEATURES             Location/Qualifiers
     source          1..17077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7191..7215)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7244..7333
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      7367..7619
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(8048..8300)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8768..9793)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8768..9793)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9803..10147)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10289..10306)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10289..10305)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10298..10320)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10445..10464)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10655..10676
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10923..10947
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12543..13691)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13990..14784)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14976..15743
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      16436..17067
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggctccacca ctagtatgaa ggagagaggt ggttcttctt cttcttcaac
     5881 aatagttctt ctttttcatt ttctagctct actttttatg gcactcttaa cttttttacc
     5941 agaaatatca gagaataccc caaaagaacc ttctaaaagg aggaaaaaac aacaaattaa
     6001 acagcagaag gagaaacaaa agcaaccatc ttcatgggat caattcaaga acctattgac
     6061 ttgcaaacaa atcgagaact caacagttca tgatcccgca tccaaaaatt ccaagttagg
     6121 ttcttgcagt tccatttgta gtttcaggga tgttgtacat ggaaacacga gagtggtaca
     6181 cagagctgat aattcaccag aaagtagcag tttaggacaa gaaactcgtc ttctaagcaa
     6241 gaataaaact tctcatcatc atggccaatc atcatcatct tcgaggtctc ttgctagatc
     6301 aaacggtagt gctagttcca cgtatactac tacttcttct tcttcaagag gaatgcaatt
     6361 tcgaaagctt tctggatgtt acgagtgtca catgatagtt gatcctagca ggtacccttt
     6421 accaaggtca accatatgtg catgccctga atgtggagaa gttttcccaa aaattgagag
     6481 cttggaacat catcaagctg ttaggcatgc tgtgtcagag ctgggtccag aagattcaag
     6541 tcgaaacata gtggagataa tcttcaaatc aagctggctc aagaaggata acccaatctg
     6601 caagatcgaa cggatattga aagtccacaa taccaaacgc acgatccaac ggttcgagga
     6661 ctgcagggac gccgtaaaga tacatgcggt cgctacaggc aagaaaaacc caagatgcgc
     6721 cgctgacgga aatgagctgc ttagatttta ctgtacaagc cttacgtgct ctctcggcgc
     6781 acgtggctcg tccagcttgt gcggctccat acctagttgt ggagtttgca ctctcatacg
     6841 tcacgggttt caaggaagca aaactagtgg agttcgcact actgctagca gtgggcgggc
     6901 ccatgactgc ctaggtagca gcatcaccaa acgtagggcg atgttggtgt gtcgggtcat
     6961 tgccggaaga gttaaacagg cagtgaaaga tgcggtggct tcgccaacga cagaggagga
     7021 gacgtgttct ggctctggtt ctggcttgtt gacaaatggc tcagtcatat acgattctgt
     7081 agccgggtat gcggggatct actcgaatct cgaggaatta tacgtattta atccaagggc
     7141 aatacttcca tgctttgtcg tgatatacga agcgttggaa tctcccgggg acccagcttt
     7201 cttgtacaaa gtggttgata acagcgggtt aattaacatc ttttacccat acgatgttcc
     7261 tgactatgcg ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt
     7321 tccagattac gctgctcagt gcagcgctta gagctcgaat ttccccgatc gttcaaacat
     7381 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     7441 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     7501 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     7561 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     7621 ggaattagct tcatcaacgc aagacatgcg cacgaccgtc tgacaggaga ggaatttccg
     7681 acgagcacag aaaggacttg ctcttggacg taggcctatt tctcaggcac atgtatcaag
     7741 tgttcggacg tgggttttcg atggtgtatc agccgccgcc aactgggaga tgaggaggct
     7801 ttcttggggg gcagtcagca gttcatttca caagacagag gaacttgtaa ggagatgcac
     7861 tgatttatct tggcgcaaac cagcaggacg aattagtggg aatagcccgc gaatatctaa
     7921 gttatgcctg tcggcatgag cagaaacttc caattcgaaa cagtttggag aggttgtttt
     7981 tgggcatacc ttttgttagt cagcctctcg attgctcatc gtcattacac agtaccgaag
     8041 tttgatcgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
     8101 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
     8161 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
     8221 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
     8281 tgccaaatgt ttgaacgatc tgcttcgacg cactccttct ttactccacc atctcgtcct
     8341 tattgaaaac gtgggtagca ccaaaacgaa tcaagtcgct ggaactgaag ttaccaatca
     8401 cgctggatga tttgccagtt ggattaatct tgcctttccc cgcatgaata atattgatga
     8461 atgcatgcgt gaggggtatt tcgattttgg caatagctgc aattgccgcg acatcctcca
     8521 acgagcataa ttcttcagaa aaatagcgat gttccatgtt gtcagggcat gcatgatgca
     8581 cgttatgagg tgacggtgct aggcagtatt ccctcaaagt ttcatagtca gtatcatatt
     8641 catcattgca ttcctgcaag agagaattga gacgcaatcc acacgctgcg gcaaccttcc
     8701 ggcgttcgtg gtctatttgc tcttggacgt tgcaaacgta agtgttggat cccggtcggc
     8761 atctactcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     8821 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     8881 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     8941 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     9001 acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     9061 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     9121 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     9181 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     9241 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     9301 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     9361 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     9421 ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt
     9481 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
     9541 cgctgaattc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
     9601 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
     9661 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
     9721 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
     9781 caggcttttt catatcgggg tcgtcctctc caaatgaaat gaacttcctt atatagagga
     9841 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
     9901 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
     9961 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
    10021 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
    10081 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
    10141 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
    10201 agtgtcgtgc tccaccatgt tgacggatct ctaggacgcg tcgacgcgtc gacgcgtcga
    10261 cgcgtcgacg cgtcctagaa gctaattcac tggccgtcgt tttacaacgt cgtgactggg
    10321 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
    10381 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
    10441 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    10501 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    10561 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    10621 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    10681 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa
    10741 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
    10801 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
    10861 aaaccacccc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
    10921 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
    10981 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
    11041 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
    11101 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
    11161 gacagagcgt tgctgcctgt gatcaaatat catctccctc gcagagatcc gaattatcag
    11221 ccttcttatt catttctcgc ttaaccgtga caggctgtcg atcttgagaa ctatgccgac
    11281 ataataggaa atcgctggat aaagccgctg aggaagctga gtggcgctat ttctttagaa
    11341 gtgaacgttg acgatatcaa ctcccctatc cattgctcac cgaatggtac aggtcgggga
    11401 cccgaagttc cgactgtcgg cctgatgcat ccccggctga tcgaccccag atctggggct
    11461 gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc ggaaccaaag
    11521 gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa cattggttcc
    11581 tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca gaagtcctcc
    11641 ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc acttgcgggt
    11701 cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga cgccgacagg
    11761 atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc cgcaaatagc
    11821 ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga tgaacacgac
    11881 catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc ggtagaccga
    11941 aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga agatgcgcat
    12001 ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac ccgccggcaa
    12061 cgcccgcagc agcataccgg cgacccctcg gcctcgctgt tcgggctcca cgaaaacgcc
    12121 ggacagatgc gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga ccgacagccc
    12181 aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc gttcagcgaa
    12241 cgcctccatg ggctttttct cctcgtgctc gtaaacggac ccgaacatct ctggagcttt
    12301 cttcagggcc gacaatcgga tctcgcggaa atcctgcacg tcggccgctc caagccgtcg
    12361 aatctgagcc ttaatcacaa ttgtcaattt taatcctctg tttatcggca gttcgtagag
    12421 cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt
    12481 tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga ccccacaagg
    12541 ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg
    12601 cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa
    12661 tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag
    12721 ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg
    12781 aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg
    12841 caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat
    12901 tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg
    12961 cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc
    13021 tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc
    13081 tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc
    13141 acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg
    13201 ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc
    13261 ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt
    13321 ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt
    13381 tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct
    13441 gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca
    13501 gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag
    13561 ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg
    13621 gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac
    13681 gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct
    13741 tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg
    13801 aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc
    13861 tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc ctgagagcca
    13921 aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac
    13981 atctaggtac taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg
    14041 cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct
    14101 gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag
    14161 atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc
    14221 gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg
    14281 cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt
    14341 attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc
    14401 cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc
    14461 agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag
    14521 cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc
    14581 ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg
    14641 gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat ataccttagc
    14701 aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc
    14761 cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag
    14821 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
    14881 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
    14941 gtatcgacgg agccgatttt gaaaccacaa ttatgggtga tgctgccaac ttactgattt
    15001 agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag ctgtccctcc
    15061 tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctat
    15121 ctctgctctc actgccgtaa aacatggcaa ctgcagttca cttacaccgc ttctcaaccc
    15181 ggtacgcacc agaaaatcat tgatatggcc atgaatggcg ttggatgccg ggcaacagcc
    15241 cgcattatgg gcgttggcct caacacgatt ttacgtcact taaaaaactc aggccgcagt
    15301 cggtaacctc gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa atggacgaac
    15361 agtggggcta tgtcggggct aaatcgcgcc agcgctggct gttttacgcg tatgacagtc
    15421 tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc
    15481 ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc tggccgctgt
    15541 atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg cagcgaattg
    15601 agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag tcgctgtcgt
    15661 tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac ataaaacact
    15721 atcaataagt tggagtcatt acccaattat gatagaattt acaagctata aggttattgt
    15781 cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct atagatatat
    15841 tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg atatcttcta
    15901 tataaaagat atattatctt atcagtattg tcaatatatt caaggcaatc tgcctcctca
    15961 tcctcttcat cctcttcgtc ttggtagctt tttaaatatg gcgcttcata gagtaattct
    16021 gtaaaggtcc aattctcgtt ttcatacctc ggtataatct tacctatcac ctcaaatggt
    16081 tcgctgggtt tatcgcaccc ccgaacacga gcacggcacc cgcgaccact atgccaagaa
    16141 tgcccaaggt aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg acggccgaag
    16201 tgaagggcag gccgccaccc aggccgccgc cctcactgcc cggcacctgg tcgctgaatg
    16261 tcgatgccag cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg ctgatcgccc
    16321 atcccgttac tgccccgatc ccggcaatgg caaggactgc cagcgctgcc atttttgggg
    16381 tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg
    16441 gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg
    16501 gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca
    16561 ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg
    16621 gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt
    16681 gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg
    16741 gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt
    16801 tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca
    16861 tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg
    16921 tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca
    16981 cacggcttcg acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg
    17041 gcgagggcaa ccagcccggt gagcgtcgga aaggcgc
//
