LOCUS       sequence-246929-        9671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, 
            Yamanaka S
  TITLE     Induction of pluripotent stem cells from adult human fibroblasts by 
            defined factors.
  JOURNAL   Cell. 2007 Nov 30. 131(5):861-72.
  PUBMED    18035408
REFERENCE   2  (bases 1 to 9671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Nov 30. 131(5):861-72."
COMMENT     Sequence Label: phREX1-Luc
FEATURES             Location/Qualifiers
     source          1..9671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1752..2207
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1839..1858)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2049..2070
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2338..2386
                     /note="synthetic polyadenylation signal"
     misc_feature    2400..2491
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2440..2459
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      7433..7442
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7439..9091
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(7505..7523)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    9132..9253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9143..9162)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9197..9216
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(9501..9518)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     1801 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     1861 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     1921 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     1981 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2041 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2101 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2161 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc attcgccatt
     2221 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc
     2281 caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat
     2341 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     2401 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     2461 agtgcaagtg caggtgccag aacatttctc tatcgatagg taccgagctc ggatccacta
     2521 gtaacggccg ccagtgtgct ggaattcgcc cttattgtcg acggggattt ggcagggtca
     2581 caggacaata gtggagggaa ggtcagcaga taagtgaaca aaggtctctg gttttcctag
     2641 gcagaggacc ctgcggcctt ccgcagtgtt tgtgtccctg ggtacttgag attagggagt
     2701 ggtgatgact cttaaggagc atgctgcctt caagcatctg tttaacaaag cacatcttgc
     2761 accaccctta atccattcaa ccctgagtgg acacagcaca tgtttcagag agcacagggt
     2821 tgggggtaag gtcacagatc aacaggatcc caaggcagaa gaatttttct tagtgcagaa
     2881 caaaatggag tctcctatgt ctacttcttt ctacacagac acagcaacca tccgatttct
     2941 caatcttttc cccacctttc ccccccttct attccacaaa accgccatgg tcatcatggc
     3001 ctgttctcaa tgagctgctg ggcacacctc ccagacgggg tggtggccgg gcagaggggc
     3061 tcctcacttc ccagtagggg cggccgggca gagacactcc tcacttccca gatgggatgg
     3121 cggccgggca gaggctgcaa tctcggctct ttgggaggcc aaggcaggcg gctgggaggt
     3181 ggaggttgta gcgagccgag atcacgccac tgcactccag cctgggcacc attgagcact
     3241 gagtgaagga gactccgtct gcaatcccgg cacctcggga ggccgaggct ggcggatcac
     3301 tcgcggttag gagctggaga ccagcccggc caacacagcg aaaccccgtc tccaccaaaa
     3361 aaatacgaaa accagtcagg cgtggcggcg cacgcctgca atcgcaggca ctcggcaggc
     3421 tgaggcagga gaatcaggca gggaggctgc agtgagccaa gatggcagca gtacagtcca
     3481 gcttcggctc ggcatcagag ggagaccgtg gaaagagagg gagagggaga ccgtggggag
     3541 agggagaggg agaccgtggg gagagggaga ccgtggggag agggagaccg tggggagagg
     3601 gagacggaga gggagaccgt ggggagaggg agaccgtggg gagagggaga gagggagagg
     3661 gatttttgtt tcattttttt cgtttgtctt tggagatgga gtctcgccct gtcgcccagg
     3721 ctgcagtgca gcggcacaat ttcggctcac tgcaaccctt gcctcctggg ttcaagcaat
     3781 tctcctgcct cagccttccg agtagctggg actacagacg cccgccacca cgactgactt
     3841 tttttttttc tttttttttt tttaatttta ttagagacag ggggtttcac catgttgccc
     3901 aggctggtct ccaactcctg agcttaggca atgtgctggc ctcggcctcc aaaagtgcta
     3961 ggattacagg catgagccac cacgcccagc caacacttgg gttctaatct gagtttagaa
     4021 acttattcct gtataaagtt tctttgtgcc tcggggttat tttaaaacgc aaatgaaatt
     4081 ataataacag taataataac tattttagaa atttgatttc agataaaatg agactgtgcg
     4141 tgtattcaat ttatattagc taaatcaaat agaaataagc tgaagctcta ataatgaaat
     4201 aatgttttac aagtttaagg ttaataagca tttatagata aggcatgttt tcttacaata
     4261 ttccgttccc agggctcctg gactgaagat cgaagttctt cccatggcct acggggtccc
     4321 agagtctggg cctgcatagc ctctccacac tcgcctccac tcatctcagg tccttccata
     4381 actattccct ctcctgtcag ggaggctctg ccgtccacgc tgattcctgc agatcaggga
     4441 aacctcccct aacttcccgg ctctcctatg tggctcccac agcaccacgc acctctcctt
     4501 cattgtgcta ataatagctg cattgtgaca cttacaatca tgattatttg gctggtatcc
     4561 tctgactgtc tttatttaca atgtggatat tttgttctct ataaattgtt gcgttgattt
     4621 tgattctttt acatgccagg ttaaaatatt actaatactg attactgagg gtgttttgtt
     4681 tacaagtgag ttactcacct caatctagtt cttaccctcc tgttctgacc tggccattcc
     4741 gcattcaaac cccaggaggc caggaatacg tatccaccag ggcctagaac ctccgcctgt
     4801 gtaggggaga aactcaaatc tgtatagagt gaattaatgc atatctatag tttttgcatt
     4861 ttaagggttt taaggaaaat attgatttgt aacacagata aaatggtcga aatacaagtg
     4921 ttttaagtga ccatgatgtt actggcagaa gaatcactat gactaaaatt ccagcaatgg
     4981 acaacacttt ttctatgcat aacctctttg tggtcaattt caagacagcc cacttctgtt
     5041 tcacggttta attggttctc gagctcattt aggagaaatt caatttcctt tgagctgagt
     5101 tcatatcaca acagatgagt tcattcgttt tggtttcttc ttcaggccca tggaaaggaa
     5161 agctaaacga taatgggagc aaaaataatc tatggaagag gtggagtgtg aaatgtaaaa
     5221 ttagattttc taaacatgct gtctctaatg cccaaacaga gccacaccca agcgatcaaa
     5281 tggaatcagt ggtaggggag gcgattataa atcaatctag tttatgggat gcactgtgtg
     5341 aatggttaag ggagtgataa agggtggttt ccgacaaccc aaagaaagga tcctcggctt
     5401 gagctaagtg tgtgtgtttc catctagtga ggaaaggggc tttcaatgac attgccactt
     5461 aaaggcttac aaagaaggtt ctggtcaaca ttcttcggtt cattgaagta tttcttatcg
     5521 aaacccaaat gttaacattt ttatctccaa gggaaacaaa aaaaaaggct ggtggaattc
     5581 ccctgtagtt tctaaagagg ccacagcaag gcgccataga gacagaagga gggaagttga
     5641 gggaagtcct tctgactggt aaatcggctt tctgaccctc gtctgcaaat aatacattct
     5701 aaggtaagga actaaggaag ctttattgaa atcggatttc aaatggagag gtcctgctat
     5761 gcctactgga ggagatcagg gaataataaa tatttccttc attgaaaatt atatcctatt
     5821 ggccaggcgc agtggctcac gcctgtaatc ccaacacttt gggaggccga gacaggcgga
     5881 tcacctgagg tcggaccagc ctgccaacat ggagaaaccc tgtctctact aaaaatacta
     5941 aattagccgt gcgtggtggt gcatgcctgt aatcccagct actggggagg ctgaggcagg
     6001 agaatcactt gaaccaaata ggcggaggtt gcagtgaggt gagacggtgc cactgcactc
     6061 cagcctgggg gacagagaaa gactccgtct caaaaaagaa aaaaaaagag aaagaaaaga
     6121 aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaaaa
     6181 ttatatttta ttggccaacc tcaaaattga agcttattca gaaacagatt taaaaaaaga
     6241 aaaaagtgga gaactgctcc cagatatata tgcagaaaag cttcaagtca acatttagga
     6301 ggtttttaaa tatcgatctg ggtttcgttc taatggtctg ggtgtactta tacgacctat
     6361 tacatttcac tagactgtag ccaatagtga gcgttgactg accattgctt tcaaagtgag
     6421 ttgtgttgcc tttagcaata cagtcacatt aatggccaga aaaagaaaaa agaaaacaaa
     6481 cactgggggt gtttgaaaca actgggggta gggtggggtg ggaaaggtgt gggggtttgc
     6541 gttcgagtca acctctattt cggggggtaa aaactgcctc ctccgcttga tttttttcct
     6601 cgatctgcaa aagcgtaaat gttttgtttt gttctattga gatggagcct cgctctatca
     6661 cccaggctgg agcttagtgg cgcgatctcg gctcactgca acctccgcat cccgggttca
     6721 agcgattctc ctgcctcagc ctcctgagta gctgggatta caggcgagca ccaccatgcc
     6781 tggctaattt ttgtattttt cggtagagac ggggtttcgc catgttggcc aggctggtct
     6841 cgaacgcttg acctcatgtg atccccccgt ctcggcctcc caaagtgctg ggacgacagg
     6901 cgtgagccac cgcgtccggc ctaaaagggt aaatgtgatt acacccacgc gtatttgttc
     6961 aacagacatt tattgagcgc tcaccacgtg ccaacgccgg gcgtctgggc tctggaggcg
     7021 ctgccacgtg gcggatgcgc agtgcccggc ggccgggctg aggggtgaac gcgggggtca
     7081 gggggccccg ggctgagggt ttgcgtgcgg gccgggtgcc tgggggcccc gggctgaggg
     7141 tgagcgcgcc gcccaggtgc caggcggctc cgggcagaga gtgaacgcgc gggccaggtg
     7201 gctccagggc ggcgccccag ggcggggccg gccacgccct ccctaaccct ggcggagctg
     7261 atgggtggct gtagcctgat tagaccgcgt cagtccggag ggtgggtctt gggagggggc
     7321 gcagggcagt ccacgtttcc actgcagttt ctcctttgtt ttacgtttgg gaggaggtgg
     7381 cattggagat ctgcgatcta agtaagcttg gcattccggt actgttggta aagccaccat
     7441 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac
     7501 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
     7561 ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt
     7621 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
     7681 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
     7741 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc
     7801 gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
     7861 aaagctccca atcatccaaa aaattattat catggattct aaaacggatt accagggatt
     7921 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
     7981 tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc
     8041 tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca
     8101 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
     8161 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
     8221 agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa
     8281 gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat
     8341 tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa
     8401 ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg
     8461 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
     8521 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
     8581 gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta
     8641 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
     8701 agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct
     8761 gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca
     8821 ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc
     8881 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
     8941 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
     9001 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
     9061 ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc ggccgcttcg
     9121 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     9181 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     9241 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     9301 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc
     9361 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     9421 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     9481 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9541 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9601 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9661 ttgctggcgt t
//
