LOCUS       sequence-246931-       15313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15313)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX074
FEATURES             Location/Qualifiers
     source          1..15313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(137..508)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            541..650
                     /note="incP origin of transfer"
     primer_bind     873..892
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1126..1143
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1331..1981)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2446..2470
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2569..2914
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3541..3717
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4220..4565
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4587..4611
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5437..5461)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5490..5579
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5613..5865
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6294..6546)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7014..8039)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7014..8039)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8049..8393)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8535..8552)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8535..8551)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8544..8566)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8691..8710)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8901..8922
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9169..9193
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10789..11937)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12236..13030)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13222..13989
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14682..15313
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ggaaaggcgc tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc
       61 gctcttcttg atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt
      121 ttagcggcta aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc
      181 caagctcgtc ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga
      241 accgcgccgt gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca
      301 ggtcgccatt gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt
      361 tgtagccctg gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct
      421 tttcctcaat cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct
      481 tcctggttgg cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag
      541 ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag
      601 tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc
      661 gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga
      721 aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg
      781 aaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      841 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      901 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      961 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1021 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1081 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1141 gaggaagcgg aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg
     1201 acgctagggc agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg
     1261 aaagggggag gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca
     1321 gcggtcctga tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc
     1381 ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg
     1441 cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg
     1501 tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag
     1561 tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc
     1621 agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg
     1681 catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc
     1741 cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg
     1801 ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa
     1861 gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc
     1921 cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca
     1981 tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa
     2041 tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat
     2101 acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat
     2161 gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta
     2221 tggcattctg ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc
     2281 gctgtcggat cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc
     2341 tggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
     2401 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
     2461 tatcctgtca aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc
     2521 ctactccaaa aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca
     2581 acaaagggta atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     2641 cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
     2701 ggctatcatt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
     2761 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     2821 catctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
     2881 tatataagga agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc
     2941 ccttaaatcg atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt
     3001 gaacgttggg atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt
     3061 ccgagttgga ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc
     3121 cttggtttca aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac
     3181 gacaggacgg aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac
     3241 tatgcagcat ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt
     3301 cgaggagtta gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg
     3361 gcaattcggt ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta
     3421 tcaagaggat gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc
     3481 gagctcgaat tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat
     3541 tttctccata aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg
     3601 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
     3661 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc
     3721 ctgcaggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
     3781 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
     3841 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
     3901 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
     3961 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
     4021 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
     4081 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
     4141 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
     4201 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
     4261 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
     4321 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
     4381 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     4441 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
     4501 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     4561 gaacacgggg gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta
     4621 gtatggctct tgaagcgttg aagtcaccta cggcggcgac gccgactcta ccaccacgtt
     4681 atgaagatgt tgatgaaatt gataatttgg attcttgggc taaaggaaaa cgatcaaaac
     4741 gaccgcgtat cgatacccca ccaactgaag aagagtattt agccctctgt ctcatcatgc
     4801 tcgctcgcag cggagccgga accggaacca gaccaggttt aactgatgct actacttccc
     4861 aacagcctgc cgataaaaaa accgtcgagt tgccgccgat tcataagaaa gaggtggcaa
     4921 cagagcaaac agagcaatct tacaagtgta gcgtgtgtga caaggctttt tcttcttatc
     4981 aagcacttgg tgggcataaa gcaagtcacc gtaaaactac tactactgcc accgccgcgt
     5041 ctgatgataa caatccttca acttcaactt ccactagcgc cgttaatatc actgctctta
     5101 atccaagtgg tcgttcacac gtctgtacta tttgccacaa ggcttttcct actggccaag
     5161 ctttgggtgg gcacaagcgc cgccactatg aaggcaaact cggtggtaac agccgcgact
     5221 taggcggcgg cggcggtcat agtggaagcg tcttgactac ttcagacgga ggcgcgtcga
     5281 ctcatacgct ccgtgacttt gaccttaaca tgcctgcttc gccggaattg caactgggtc
     5341 tgagtattga ctgtggacgg aaaagtcaac tgttgccgat tgaccaagag gtggaaagtc
     5401 ctatgcctgc aaagaaaccg cgtttattgc ccggggaccc agctttcttg tacaaagtgg
     5461 ttgataacag cgggttaatt aacatctttt acccatacga tgttcctgac tatgcgggct
     5521 atccctatga cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg
     5581 ctcagtgcag cgcttagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     5641 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     5701 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     5761 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     5821 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttagcttcat
     5881 caacgcaaga catgcgcacg accgtctgac aggagaggaa tttccgacga gcacagaaag
     5941 gacttgctct tggacgtagg cctatttctc aggcacatgt atcaagtgtt cggacgtggg
     6001 ttttcgatgg tgtatcagcc gccgccaact gggagatgag gaggctttct tggggggcag
     6061 tcagcagttc atttcacaag acagaggaac ttgtaaggag atgcactgat ttatcttggc
     6121 gcaaaccagc aggacgaatt agtgggaata gcccgcgaat atctaagtta tgcctgtcgg
     6181 catgagcaga aacttccaat tcgaaacagt ttggagaggt tgtttttggg catacctttt
     6241 gttagtcagc ctctcgattg ctcatcgtca ttacacagta ccgaagtttg atcgatctag
     6301 taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc
     6361 tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa
     6421 cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
     6481 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
     6541 acgatctgct tcgacgcact ccttctttac tccaccatct cgtccttatt gaaaacgtgg
     6601 gtagcaccaa aacgaatcaa gtcgctggaa ctgaagttac caatcacgct ggatgatttg
     6661 ccagttggat taatcttgcc tttccccgca tgaataatat tgatgaatgc atgcgtgagg
     6721 ggtatttcga ttttggcaat agctgcaatt gccgcgacat cctccaacga gcataattct
     6781 tcagaaaaat agcgatgttc catgttgtca gggcatgcat gatgcacgtt atgaggtgac
     6841 ggtgctaggc agtattccct caaagtttca tagtcagtat catattcatc attgcattcc
     6901 tgcaagagag aattgagacg caatccacac gctgcggcaa ccttccggcg ttcgtggtct
     6961 atttgctctt ggacgttgca aacgtaagtg ttggatcccg gtcggcatct actctattcc
     7021 tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag
     7081 ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct
     7141 ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg
     7201 tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc
     7261 ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa
     7321 gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc
     7381 gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag
     7441 ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc
     7501 tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga
     7561 tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt
     7621 ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca
     7681 tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc
     7741 aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca
     7801 atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga
     7861 tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca
     7921 tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg
     7981 tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata
     8041 tcggggtcgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
     8101 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
     8161 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     8221 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
     8281 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
     8341 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
     8401 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
     8461 ccatgttgac ggatctctag gacgcgtcga cgcgtcgacg cgtcgacgcg tcgacgcgtc
     8521 ctagaagcta attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     8581 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     8641 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgcccg ctcctttcgc
     8701 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     8761 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     8821 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     8881 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     8941 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag
     9001 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
     9061 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccccagta
     9121 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     9181 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     9241 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     9301 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     9361 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     9421 gcctgtgatc aaatatcatc tccctcgcag agatccgaat tatcagcctt cttattcatt
     9481 tctcgcttaa ccgtgacagg ctgtcgatct tgagaactat gccgacataa taggaaatcg
     9541 ctggataaag ccgctgagga agctgagtgg cgctatttct ttagaagtga acgttgacga
     9601 tatcaactcc cctatccatt gctcaccgaa tggtacaggt cggggacccg aagttccgac
     9661 tgtcggcctg atgcatcccc ggctgatcga ccccagatct ggggctgaga aagcccagta
     9721 aggaaacaac tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag taggttaaac
     9781 ccgctccgat caggccgagc cacgccaggc cgagaacatt ggttcctgta ggcatcggga
     9841 ttggcggatc aaacactaaa gctactggaa cgagcagaag tcctccggcc gccagttgcc
     9901 aggcggtaaa ggtgagcaga ggcacgggag gttgccactt gcgggtcagc acggttccga
     9961 acgccatgga aaccgccccc gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt
    10021 ttggtgtcaa caccaacagc gccacgcccg cagttccgca aatagccccc aggaccgcca
    10081 tcaatcgtat cgggctacct agcagagcgg cagagatgaa cacgaccatc agcggctgca
    10141 cagcgcctac cgtcgccgcg accccgcccg gcaggcggta gaccgaaata aacaacaagc
    10201 tccagaatag cgaaatatta agtgcgccga ggatgaagat gcgcatccac cagattcccg
    10261 ttggaatctg tcggacgatc atcacgagca ataaacccgc cggcaacgcc cgcagcagca
    10321 taccggcgac ccctcggcct cgctgttcgg gctccacgaa aacgccggac agatgcgcct
    10381 tgtgagcgtc cttggggccg tcctcctgtt tgaagaccga cagcccaatg atctcgccgt
    10441 cgatgtaggc gccgaatgcc acggcatctc gcaaccgttc agcgaacgcc tccatgggct
    10501 ttttctcctc gtgctcgtaa acggacccga acatctctgg agctttcttc agggccgaca
    10561 atcggatctc gcggaaatcc tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa
    10621 tcacaattgt caattttaat cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc
    10681 cgagcgatac tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag
    10741 taaagcgctg gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca
    10801 atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc
    10861 gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat
    10921 gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa
    10981 catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg
    11041 gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt
    11101 cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac
    11161 gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt
    11221 cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa
    11281 ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag
    11341 ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac
    11401 gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa
    11461 cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat
    11521 atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc
    11581 ctgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt
    11641 cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc
    11701 gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg
    11761 cacgctgtcg cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa
    11821 ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga
    11881 aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca
    11941 ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga
    12001 cccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta
    12061 gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag
    12121 cgaccccttg cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg
    12181 gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa
    12241 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
    12301 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
    12361 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
    12421 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
    12481 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
    12541 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
    12601 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
    12661 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
    12721 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
    12781 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
    12841 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
    12901 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
    12961 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
    13021 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
    13081 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
    13141 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
    13201 gattttgaaa ccacaattat gggtgatgct gccaacttac tgatttagtg tatgatggtg
    13261 tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga
    13321 cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg
    13381 ccgtaaaaca tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa
    13441 aatcattgat atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt
    13501 tggcctcaac acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc
    13561 atacagccgg gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggctatgtc
    13621 ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg acagtctccg gaagacggtt
    13681 gttgcgcacg tattcggtga acgcactatg gcgacgctgg ggcgtcttat gagcctgctg
    13741 tcaccctttg acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg
    13801 aagggaaagc tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg
    13861 aatctgaggc agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg
    13921 gagctgcatg acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga
    13981 gtcattaccc aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc
    14041 attagtccat gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg
    14101 cctatgcctt gccccctgaa atccttacat acggcgatat cttctatata aaagatatat
    14161 tatcttatca gtattgtcaa tatattcaag gcaatctgcc tcctcatcct cttcatcctc
    14221 ttcgtcttgg tagcttttta aatatggcgc ttcatagagt aattctgtaa aggtccaatt
    14281 ctcgttttca tacctcggta taatcttacc tatcacctca aatggttcgc tgggtttatc
    14341 gcacccccga acacgagcac ggcacccgcg accactatgc caagaatgcc caaggtaaaa
    14401 attgccggcc ccgccatgaa gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg
    14461 ccacccaggc cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc
    14521 tgcggcacgt caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc
    14581 ccgatcccgg caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg
    14641 gccgaggggc gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg
    14701 agaagggggg gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt
    14761 aaaaacaagg tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc
    14821 gaaaaacggg cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa
    14881 tgtcaatagg tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc
    14941 ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag
    15001 gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga
    15061 ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc
    15121 gccgggtgag tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa
    15181 gttttccgcg aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg
    15241 cgtttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag
    15301 cccggtgagc gtc
//
